Assays Cell
The Violet Ratiometric Membrane Asymmetry Probe⁄Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry. The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis. The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 ...
Catalog: A-35137
Size: 1 kit
Size: 1 kit
The LIVE/DEAD® Fixable Dead Cell Stain Kit employs an amine-reactive fluorescent dye to evaluate mammalian cell viability by flow cytometry. In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity between the live and dead cell populations is typically greater than 50 fold, and this fluorescence intensity discrimination is completely preserved fol...
Catalog: L-23105
Size: 1 kit
Size: 1 kit
The Tali® Cell Cycle Kit includes a ready-to-use reagent that, when used with the Tali® Image-Based Cytometer, provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. This single-addition reagent is composed of propidium iodide (PI), RNase A, and Triton X-100. The Tali® Cell Cycle Kit offers a simple workflow: • The pre-mixed reagent can be stored at room temperature and used when your cells are ready • Simply fix your cells, add the reagent, incubate, and visualize • Cell cycle data can be analyzed on the Tali® instrument or exported as a ....
Catalog: A10798
Size: 50 assays
Size: 50 assays
For Academic pricing please login or contact us at discoverysciences@invitrogen.com. The Tango™ CCR5- bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 5 (CCR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR- bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase ( bla ) reporter gene under the control of a UAS response element. The Tango™ CCR5- bla U2OS cells are functionally validated for Z' and EC 50 concentrations of MIP-1a. In addition,...
Catalog: K1788
Size: 8 × 10^6 cells
Size: 8 × 10^6 cells
The Vybrant® Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types. In this assay, cells are labeled with calcein AM and allowed to adhere. After removal of nonadherent cells, calcein fluorescence is used to calculate the number of adherent cells.
Catalog: V-13181
Size: 1 kit
Size: 1 kit
The CellROX® Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX® Deep Red Reagent, as well as SYTOX® Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS). The CellROX® Deep Red Flow Cytometry Assay Kit features: • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines,...
Catalog: C10491
Size: 100 assays
Size: 100 assays
The CellROX® Orange Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX® Orange Reagent, as well as SYTOX® Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS). The CellROX® Orange Flow Cytometry Assay Kit features: • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowi...
Catalog: C10493
Size: 100 assays
Size: 100 assays
The CellROX® Green Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX® Green Reagent, as well as SYTOX® Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS). The CellROX® Green Flow Cytometry Assay Kit features: • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing ...
Catalog: C10492
Size: 100 assays
Size: 100 assays
The Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit contains a sensitive, one-step assay that uses the Amplex® Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) to detect hydrogen peroxide (H 2 O 2 ) or peroxidase activity. The Amplex® Red reagent, in combination with horseradish peroxidase (HRP), has been used to detect H 2 O 2 released from biological samples, including cells, or generated in enzyme-coupled reactions. Furthermore, Amplex® Red reagent can be used as an ultrasensitive assay for peroxidase activity when H 2 O 2 is in excess. In the presence of peroxidase, the Amplex® Red reag...
Catalog: A22188
Size: 1 kit
Size: 1 kit
The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent® Caspase-3/7 Green Detection Reagent, as well as SYTOX® AADvanced™ Dead Cell Stain. • Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7 • Easy identification—clearly identify live, dead, and apoptotic cell populations • Quick analysis—washing and fixation is not required • Multicolor compatibility—combine with other dyes excitable by the 488 n...
Catalog: C10427
Size: 100 assays
Size: 100 assays
The Vybrant Apoptosis Assay Kit #4 detects apoptosis on the basis of changes that occur in the permeability of cell membranes. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide nucleic acid stains. YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence. Necrotic cells are stained red-fluorescent with propidium iodide.
Catalog: V13243
Size: 1 kit
Size: 1 kit
Assays Cell
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