Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Size: 100 ml
The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Size: 250 µl
SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. • Impermeant to live cells • Excitation/Emission: 504/523 nm • Use with 488 Argon-ion laser • Works with mammalian cells and both Gram-positive and Gram-negative bacteria Simple Viability Determination SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, ...
Size: 250 µl
The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.
Size: 250 µl
FxCycle™ PI/RNase Staining Solution is used for flow cyotmetric analysis of DNA content in fixed cells. Propidium iodide (PI), a popular red-fluorescent stain, binds to DNA by intercalating between the bases with little or no sequence preference and with a stoichiometry of one dye per 4–5 base pairs of DNA. PI also binds to RNA, necessitating treatment with RNase to distinguish between RNA and DNA staining. This propidium iodide solution includes DNase-free RNase A and a permeablization reagent. It comes ready to use: simply add the staining solution to fixed cells, incubate, and acquire on a ...
Size: 100 ml
The high-affinity nucleic acid stain BOBO™-1 has excitation/emission maxima ~462/481 nm when bound to double-stranded DNA in aqueous solution. This cyanine dimer is cell-impermeant.
Size: 200 µl
THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.4 +/- 0.2 buffer is provided in one bottle containing 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution i...
Size: 50 ml
Propidium iodide is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.
Size: 10 ml
Clone/PAD: PY20 Immunogen: Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine Specificity: Reacts specifically with tyrosine phosphorylated proteins in the native or denatured states, and does not react with phosphoserine or phosphothreonine. Reacts with a wide variety of tyrosine-phosphorylated proteins in all tested species. Reactivity: Human, Mouse, (positive controls: mouse brain, NIH 3T3 cells (+/- TPA), K562 cells, EGF-stimulated A431 cells, and MCF-7 cell lysates) Applications: Western blotting, Immunoprecipitation, ELISA, Affinity chromotograp...
Size: 100 µg
Ambion® GlycoBlue™ consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate and is useful as a nucleic acid coprecipitant. Ideal coprecipitant in nuclease protection assays at 1/100 dilution of stock solution, it is provided in five tubes of 0.3 mL each at a concentration of 15 mg/mL. • Ideal for RT-PCR • Increases pellet mass • Quantitative recovery of low concentrations (ng/mL) of nucleic acid • Prevents pellet loss in nuclease protection assays What is a Coprecipitant? Coprecipitants are inert substances used to aid recovery of nucleic acids during alcohol precipi...
Size: 5 × 300 µl