Alkaline Phosphatase Activity Assay

Alkaline Phosphatase Live Stain

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

Alkaline Phosphatase Live Stain is a stem cell imaging product that allows users to differentially stain pluripotent stem cells (PSCs). The dye is a cell-permeable fluorescent substrate for alkaline phosphatase (AP) that is non-toxic to cells, diffusing out over the course of two hours. The Alkaline Phosphatase Live Stain consists of 50 µL of a 500X fluorescein-based dye in DMSO, sufficient for staining two 24-well plates, four 6 cm dishes or two 10 cm dishes. This Alkaline Phosphatase Live Stain allows you to: • Identify pluripotent stem cells with a strong signal above background • Stain PSC...
Catalog: A14353
Size: 50 µl

RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit - one 96-Well Microplate

Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Phosphatase Assay Kits

The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensur...
Catalog: R33700
Size: 1 kit

NA-Fluor™ Influenza Neuraminidase Assay Kit

Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Other Enzyme Assay Kits

The NA-Fluor™ Influenza Neuraminidase Assay Kit provides validated reagents and a standardized protocol for conducting neuraminidase enzyme assays, including neuraminidase inhibitor (NI) susceptibility screening using a fluorescent MUNANA substrate. Key product features: • Efficient design—neuraminidase assay in one complete kit • Optimized formulations—assay reagents have been optimized for maximum performance based on NISN protocols • Robust application—functional detection of NI-resistant virus in mixed viral populations • Easy of use & flexible screening—stable fluorescent signal enables b...
Catalog: 4457091
Size: 960 assays

EnzChek® Lysozyme Assay Kit - 400 Assays

Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Other Enzyme Assay Kits

The EnzChek® Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme acitivty in solution. The assay measures lysozyme activity on Micrococcus lysodeikticus cell walls, which are labeled to such a degree with fluorescein that the fluorescence is quenched. Lysozyme action can relieve this quenching, yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.
Catalog: E22013
Size: 1 kit

Goat Anti-Mouse IgG (H+L) - AP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Immunogen: Mouse IgG (H+L) Specificity: Mouse IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background. Enhanced sensitivity,...
Catalog: 62-6522
Size: 1 ml

Phosphotyrosine [pY] Mouse Monoclonal Antibody (clone PY20), AP Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Anti-Phospho Amino Acid Antibodies

Clone/PAD: PY20 Immunogen: Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine Specificity: Reacts specifically with tyrosine phosphorylated proteins in the native or denatured states, and does not react with phosphoserine or phosphothreonine. Reacts with a wide variety of tyrosine-phosphorylated proteins in all tested species. Reactivity: Human, Mouse, (positive controls: mouse brain, NIH 3T3 cells (+/- TPA), K562 cells, EGF-stimulated A431 cells, and MCF-7 cell lysates) Applications: Western blotting, Immunoprecipitation, ELISA, Affinity chromotograp...
Catalog: 03-7722
Size: 100 µg

LanthaScreen® TR-FRET AR Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4381: The LanthaScreen™ TR-FRET Androgen Receptor (AR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential AR ligands as agonists of coactivator recruitment or antagonists of agonist-dependent coactivator recruitment. In both the agonist and antagonist modes, the homogeneous mix-and-read assay uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a rat AR ligand-binding domain (AR-LBD) that is tagged with hexahisti...
Catalog: A15119
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET CAR Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4836: The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of liganddependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneo...
Catalog: A15141
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET ER alpha Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4544: The LanthaScreen™ TR-FRET ER alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential estrogen receptor (ER) alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ER alpha ligand-binding domain (ER alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. Agonist m...
Catalog: A15125
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET ER beta Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4541: The LanthaScreen™ TR-FRET Estrogen Receptor beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ER beta ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a ER beta ligand-binding domain (ER beta-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. Agonist mode To run...
Catalog: A15124
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET ERR alpha Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4663: The LanthaScreen™ ERR (estrogen related receptor) alpha TR-FRET Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential ERR alpha ligands as inverse agonists (sometimes referred to as antagonists) of coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ERR alpha ligand-binding domain (ERR alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-rea...
Catalog: A15130
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET ERR beta Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4800: The LanthaScreen™ TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant ERR beta ligand-binding domain (ERR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay...
Catalog: A15139
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET ERR gamma Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4408: The LanthaScreen™ TR-FRET Estrogen Related Receptor (ERR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ERR gamma ligands as antagonists/inverse agonists of coactivator displacement. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1). How it works The LanthaScreen™ TR...
Catalog: A15121
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET FXR Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4833: The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein- labeled coactivator peptide, and an FXR ligand-binding domain (FXR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous, mix-and-read assay format. To assay: When running the LanthaScreen™ TR-FRET FXR co...
Catalog: A15140
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET GR Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4683: The LanthaScreen™ TR-FRET GR (glucocorticoid receptor) Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential GR ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant GR ligand-binding domain (GR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format. Agonist mode: ...
Catalog: A15132
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET LXR alpha Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4655: The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR alpha ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format. In the LanthaScreen™ TR-FRET LXR alpha Coactivator ...
Catalog: A15128
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET LXR beta Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4658: The LanthaScreen™ TR-FRET LXR (liver X receptor) beta Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR beta ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR beta ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format. How it works: In the LanthaScreen™ TR-FRET LXR beta Co...
Catalog: A15129
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET PPAR alpha Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4684: The LanthaScreen™ TR-FRET PPAR (peroxisome proliferator activated receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential PPAR alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR alpha ligand-binding domain (PPAR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a ho...
Catalog: A15133
Size: 800 × 20 µl assays

LanthaScreen® TR-FRET PPAR alpha Competitive Binding Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4892: The LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay provides a sensitive and robust method for high-throughput screening (HTS) of ligands for peroxisome proliferator-activated receptor alpha (PPAR alpha). The kit uses a terbium-labeled anti-GST antibody, a fluorescent small-molecule pan-PPAR ligand (Fluormone™ Pan-PPAR Green), and a human PPAR alpha ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), in a homogeneous mix-and-read assay format. To assay: When r...
Catalog: A15143
Size: 400 × 40 µl assays

LanthaScreen® TR-FRET PPAR delta Coactivator Assay Kit (Rabbit)

Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4685: The LanthaScreen™ TR-FRET PPAR (peroxisome proliferator activated receptor) delta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential PPAR delta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR delta ligand-binding domain (PPAR delta-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous ...
Catalog: A15134
Size: 800 × 20 µl assays