Alexa Fluor Hcs Detection Kits

FISH Tag™ DNA Multicolor Kit, Alexa Fluor® dye combination

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Fluorescent Nucleic Acid Detection (FISH) Kits

The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors. FISH Tag™ Kit Specificati...
Catalog: F32951
Size: 1 kit

FISH Tag™ DNA Green Kit, with Alexa Fluor® 488 dye

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Fluorescent Nucleic Acid Detection (FISH) Kits

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit , which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. FISH Tag™ Kit Specificatio...
Catalog: F32947
Size: 1 kit

Alexa Fluor® 488 Protein Labeling Kit

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits

Molecular Probes® Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, FISH, and more. Kits are available in 12 Alexa Fluor® dye colors, biotin, the hapten Oregon Green® 488, fluorescein EX, and Texas Red® dye. Each kit provides the components needed to perform three protein conjugations and purifications. Important Features of Protein La...
Catalog: A-10235
Size: 1 kit

Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits

The Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 594 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 594 azide leads to a chemoselective ligation or “click" reaction between the...
Catalog: C10429
Size: 1 kit

Alexa Fluor® 488 Carboxylic Acid, 2,3,5,6-Tetrafluorophenyl Ester (Alexa Fluor® 488 5-TFP), 5-isomer

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Amine-Reactive Fluorophores, Biotins, Quantum Dots, & Other Labels

Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
Catalog: A-30005
Size: 1 mg

Click-iT® HPG Alexa Fluor® 488 Protein Synthesis Assay Kit

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits

The Click-iT® HPG Alexa Fluor® 488 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 488 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 488 azide leads to a chemoselective ligation or "click" reaction between the...
Catalog: C10428
Size: 1 kit

Alexa Fluor® 647 C 2 Maleimide

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels

Conjugates prepared with the thiol-reactive Alexa Fluor 647® C 2 maleimide are spectrally similar to the Cy5 dye, resulting in an optimal match to optical filters for the Cy5 dye. Total fluorescence of secondary antibody conjugates of Alexa Fluor® 647 dye, however, is significantly higher than that of Cy5 conjugates. Unlike the Cy5 dye, Alexa Fluor® 647 dye has very little change in absorption of fluorescence spectra when conjugated to most porteins, oligonucleotides, or nucleic acids, yielding greater total fluorescence at the same degree of substitution.
Catalog: A-20347
Size: 1 mg

Alexa Fluor® 546 C 5 Maleimide

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels

The thiol-reactive Alexa Fluor 546 C 5 maleimide and its conjugates are perfect for applications that require orange-fluorescent probes. Spectrally similar to the Cy3 dye, the Alexa Fluor 546 dye is readily excited by the 546 nm line of the mercury-arc lamp and the 543 nm line of the He-Ne laser.
Catalog: A-10258
Size: 1 mg

Alexa Fluor® 488 hydrazide, Sodium Salt, 1 mg

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Aldehyde-, Ketone-, & Carboxylate-Reactive Labels

The Alexa Fluor 488 hydrazide is a low molecular weight, cell membrane—impermeant, aldehyde-fixable molecule that can be used as a cell tracer. This green-fluorescent polar dye can be loaded into cells by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: A-10436
Size: 1 mg

Alexa Fluor® 488 C 5 Maleimide

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels

Conjugates prepared with the thiol-reactive Alexa Fluor 488 C 5 maleimide are significantly brighter and more photostable than fluorescein. The bright, green fluorescence of the Alexa Fluor 488 dye, which is spectrally similar to that of fluorescein, is pH independent from pH 4 to 10.
Catalog: A-10254
Size: 1 mg

Alexa Fluor® 430 Carboxylic Acid, Succinimidyl Ester

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Amine-Reactive Fluorophores, Biotins, Quantum Dots, & Other Labels

The amine-reactive Alexa Fluor 430 carboxylic acid, succinimidyl ester and its conjugates exhibit the rare combination of absorption between 400 nm and 450 nm and fluorescence emission beyond 500 nm (excitation/emission maxima ~434/539 nm).
Catalog: A-10169
Size: 5 mg