Alexa Fluor® 594
Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis. ChromaTide® Labeled Nucleotides Specifications: • Ex/Em of dye: Alexa Fluor® 594-5-dUTP (590/615 nm) • L...
Catalog: C-11400
Size: 25 µl
Size: 25 µl
This highly cross-adsorbed Alexa Fluor® 594 Goat Anti-Rabbit IgG (H+L) is labeled with our superior red-fluorescent Alexa Fluor 594 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG and human IgG.
Catalog: A-11037
Size: 0.5 ml
Size: 0.5 ml
The Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 594 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 594 azide leads to a chemoselective ligation or “click" reaction between the...
Catalog: C10429
Size: 1 kit
Size: 1 kit
The Alexa Fluor 594 chicken anti—mouse IgG is labeled with our bright, red-fluorescent Alexa Fluor 594 dye (which is spectrally similar to Texas Red dye) and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. Chicken antibodies lack a classic "Fc" domain and will not bind to mammalian IgG Fc receptors. To minimize crossreactivity, the antibody has been adsorbed against human and rabbit IgG prior to conjugation.
Catalog: A-21201
Size: 0.5 ml
Size: 0.5 ml
Alexa Fluor® 594 Donkey Anti-Goat IgG (H+L) is labeled with our superior, red-fluorescent Alexa Fluor 594 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from goat. To minimize cross-reactivity, it has been adsorbed against rabbit, rat, mouse, and human IgG.
Catalog: A-11058
Size: 0.5 ml
Size: 0.5 ml
The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors. FISH Tag™ Kit Specificati...
Catalog: F32951
Size: 1 kit
Size: 1 kit
Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
Catalog: A-30005
Size: 1 mg
Size: 1 mg
Alexa Fluor 488 rabbit anti—rat IgG is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rat. To minimize crossreactivity, it has been adsorbed against human IgG prior to conjugation.
Catalog: A-21210
Size: 0.5 ml
Size: 0.5 ml
The Alexa Fluor® 488 goat anti-mouse IgG 1 is labeled with our exceptionally bright, photostable Alexa Fluor® 488 dye (which is spectrally similar to fluorescein) and is prepared from affinity-purified antibodies that react with the Fc portion of the heavy chain of mouse IgG 1 . To minimize crossreactivity, the antibody has been adsorbed against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG 2a , IgG 2b and IgG 3 prior to labeling.
Catalog: A-21121
Size: 250 µl
Size: 250 µl
Alexa Fluor® 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 647 dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 650/668 nm Superior to antibody staining Optimal for fixed and permeabilized samples Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has...
Catalog: A22287
Size: 300 units
Size: 300 units
The Alexa Fluor 488 Oligonucleotide Amine Labeling Kit provides a simple method for labeling amine-modified oligonucleotides with our bright, photostable, green-fluorescent Alexa Fluor 488 dye (which is spectrally similar to fluorescein). Oligonucleotides labeled with this kit can be used as primers and as probes in hybridization experiments but should not be used for automated DNA sequencing since the Alexa Fluor dye in the kit is supplied as mixed isomers. The kits have been optimized for labeling reactions with 50 µg of a 5'-amine—modified oligonucleotide, 18 to 24 bases in length.
Catalog: A-20191
Size: 1 kit
Size: 1 kit
FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit , which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. FISH Tag™ Kit Specificatio...
Catalog: F32947
Size: 1 kit
Size: 1 kit
The Alexa Fluor 633 donkey anti—goat IgG is labeled with our bright, far-red—fluorescent Alexa Fluor 633 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from goat. To minimize crossreactivity, it has been adsorbed against rabbit, rat, mouse and human IgG. Fluorescence of this long-wavelength Alexa Fluor dye is not visible to the human eye but is readily detected by most imaging systems.
Catalog: A-21082
Size: 0.5 ml
Size: 0.5 ml
Molecular Probes® Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, FISH, and more. Kits are available in 12 Alexa Fluor® dye colors, biotin, the hapten Oregon Green® 488, fluorescein EX, and Texas Red® dye. Each kit provides the components needed to perform three protein conjugations and purifications. Important Features of Protein La...
Catalog: A-10235
Size: 1 kit
Size: 1 kit
Conjugates prepared with the thiol-reactive Alexa Fluor 647® C 2 maleimide are spectrally similar to the Cy5 dye, resulting in an optimal match to optical filters for the Cy5 dye. Total fluorescence of secondary antibody conjugates of Alexa Fluor® 647 dye, however, is significantly higher than that of Cy5 conjugates. Unlike the Cy5 dye, Alexa Fluor® 647 dye has very little change in absorption of fluorescence spectra when conjugated to most porteins, oligonucleotides, or nucleic acids, yielding greater total fluorescence at the same degree of substitution.
Catalog: A-20347
Size: 1 mg
Size: 1 mg
Alexa Fluor 555 goat anti—rabbit IgG is labeled with our exceptionally bright, orange-fluorescent Alexa Fluor 555 dye (which is spectrally similar to Cy3 dye) and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize crossreactivity, it has been adsorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum. We also provide a version of the same antibody that has been cross-adsorbed against additional species (Cat. no. A-21429).
Catalog: A-21428
Size: 0.5 ml
Size: 0.5 ml
Alexa Fluor 647 F(ab') 2 fragment of goat anti—rabbit IgG is labeled with our exceptionally bright, far-red—fluorescent Alexa Fluor 647 dye (which is spectrally similar to Cy5 dye) and is prepared from antibodies that have been adsorbed against pooled human serum, mouse serum, mouse plasmacytoma/hybridoma proteins and purified human paraproteins to minimize crossreactivity. Conjugates of F(ab') 2 fragments are sometimes preferable to whole antibody conjugates for secondary detection, since the absence of the Fc region in F(ab') 2 fragments prevents interactions with Fc receptor-bearing membran...
Catalog: A-21246
Size: 250 µl
Size: 250 µl
Alexa Fluor® 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western ...
Catalog: S-32354
Size: 0.5 ml
Size: 0.5 ml
Mouse monoclonal HP6069; specific for the Fc region of human IgG1 and conjugated to the bright, photostable Alexa Fluor 488 dye. Replaces product number 053311.
Catalog: A-10631
Size: 250 µl
Size: 250 µl
Alexa Fluor® 488 Goat Anti-Rat IgG (H+L) is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rat. To minimize cross-reactivity, it has been adsorbed against mouse IgG, mouse serum and human serum prior to conjugation.
Catalog: A-11006
Size: 0.5 ml
Size: 0.5 ml
Alexa Fluor® 594
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