Alexa Fluor® 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 647 dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 650/668 nm Superior to antibody staining Optimal for fixed and permeabilized samples Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has...
Size: 300 units
Conjugates prepared with the thiol-reactive Alexa Fluor 647® C 2 maleimide are spectrally similar to the Cy5 dye, resulting in an optimal match to optical filters for the Cy5 dye. Total fluorescence of secondary antibody conjugates of Alexa Fluor® 647 dye, however, is significantly higher than that of Cy5 conjugates. Unlike the Cy5 dye, Alexa Fluor® 647 dye has very little change in absorption of fluorescence spectra when conjugated to most porteins, oligonucleotides, or nucleic acids, yielding greater total fluorescence at the same degree of substitution.
Size: 1 mg
Alexa Fluor 647 F(ab') 2 fragment of goat anti—rabbit IgG is labeled with our exceptionally bright, far-red—fluorescent Alexa Fluor 647 dye (which is spectrally similar to Cy5 dye) and is prepared from antibodies that have been adsorbed against pooled human serum, mouse serum, mouse plasmacytoma/hybridoma proteins and purified human paraproteins to minimize crossreactivity. Conjugates of F(ab') 2 fragments are sometimes preferable to whole antibody conjugates for secondary detection, since the absence of the Fc region in F(ab') 2 fragments prevents interactions with Fc receptor-bearing membran...
Size: 250 µl
The CD54 (ICAM-1), clone YN1/1.7.4, Alexa Fluor® 647 dye conjugate is a rat monoclonal antibody that detects mouse CD54 (ICAM-1). The Alexa Fluor® 647 dye conjugate provides an excellent spectral match to the common long-wavelength excitation sources, with a high extinction coefficient. The fluorescence emission of these conjugates virtually matches that of Cy®5 dye, so optical filters designed for Cy®5 dye are ideal. Antibody Specifications Description: CD54 (ICAM-1), rat IgG2bκ monoclonal Label: Alexa Fluor® 647 dye Validated Application: Flow Cytometry Species Reactivity: Mouse Ex/Em and la...
Size: 0.1 mg
Alexa Fluor® 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western ...
Size: 1 mg
The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors. FISH Tag™ Kit Specificati...
Size: 1 kit
Molecular Probes® cholera toxin conjugates are made from a recombinant version of the B subunit only. This allows us to provide a very high-purity product that is completely free of the toxic A subunit. Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1 , making it a powerful tool for retrograde labeling of neurons. This tracer has been used in a variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall. When used in neuronal tracing applications, CT-B is typically introduc...
Size: 100 µg
Lectin phytohemagglutinin-L (PHA-L) is strongly inhibited by N-acetylglucosamine β(1-2) mannopyranosyl residues.[ALTREF PN29369] This lectin has been shown to agglutinate leukocytes and induce mitogenic activity in T-lymphocytes[ALTREF PN29369,PN29368] and has also been used as an anterograde tracer for the study of neuronal pathways.[ALTREF PN20284]
Size: 1 mg