aldehyde-, ketone- & carboxylate-reactive labels
The Alexa Fluor 488 hydrazide is a low molecular weight, cell membrane—impermeant, aldehyde-fixable molecule that can be used as a cell tracer. This green-fluorescent polar dye can be loaded into cells by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: A-10436
Size: 1 mg
Size: 1 mg
Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
Catalog: A-30005
Size: 1 mg
Size: 1 mg
The amine-reactive Alexa Fluor 430 carboxylic acid, succinimidyl ester and its conjugates exhibit the rare combination of absorption between 400 nm and 450 nm and fluorescence emission beyond 500 nm (excitation/emission maxima ~434/539 nm).
Catalog: A-10169
Size: 5 mg
Size: 5 mg
This single 5-isomer preparation of our proprietary, amine-reactive dye, Oregon Green 488 carboxylic acid, succinimidyl ester and its conjugates have green fluorescence similar to that of fluorescein but are more photostable. Additionally, Oregon Green 488 dye has a lower pK a than fluorescein (pK a = 4.7 versus 6.4 for fluorescein), making this bright dye less pH sensitive in the physiological range.
Catalog: O-6147
Size: 5 mg
Size: 5 mg
The Alexa Fluor 488 Oligonucleotide Amine Labeling Kit provides a simple method for labeling amine-modified oligonucleotides with our bright, photostable, green-fluorescent Alexa Fluor 488 dye (which is spectrally similar to fluorescein). Oligonucleotides labeled with this kit can be used as primers and as probes in hybridization experiments but should not be used for automated DNA sequencing since the Alexa Fluor dye in the kit is supplied as mixed isomers. The kits have been optimized for labeling reactions with 50 µg of a 5'-amine—modified oligonucleotide, 18 to 24 bases in length.
Catalog: A-20191
Size: 1 kit
Size: 1 kit
The FluoReporter® FITC Protein Labeling Kit provides a convenient and flexible means for attaching fluorescein (FITC, Ex/Em ~494/518 nm) to your monoclonal or polyclonal antibody (or any protein >30 kDa). The kit supplies the reagents needed for 5 to 10 labeling and separation reactions. Important Features of the FluoReporter® FITC Protein Labeling Kit: • Labeled proteins typically ready to use in under 3 hours with very little hands-on time • Optimized for 0.2–2.0 mg of protein with molecular weight >30 kDa • Labeled proteins are purified using convenient spin filters • Requires the antibody ...
Catalog: F-6434
Size: 1 kit
Size: 1 kit
The Vybrant® DiD cell-labeling solution is a dye delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells for use in cell-cell fusion, cellular adhesion and migration applications.
Catalog: V-22887
Size: 1 ml
Size: 1 ml
Molecular Probes® Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, FISH, and more. Kits are available in 12 Alexa Fluor® dye colors, biotin, the hapten Oregon Green® 488, fluorescein EX, and Texas Red® dye. Each kit provides the components needed to perform three protein conjugations and purifications. Important Features of Protein La...
Catalog: A-10235
Size: 1 kit
Size: 1 kit
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solid (D-282), as a paste (N-22880) or as crystals (D-3911).
Catalog: V-22885
Size: 1 ml
Size: 1 ml
The Vybrant® CM-DiI cell-labeling solution is a dye delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells for use in cell-cell fusion, cellular adhesion and migration applications.
Catalog: V-22888
Size: 1 ml
Size: 1 ml
To permit simultaneous long-term tracing of mixed-cell populations using different fluorescent colors, we have developed a probe whose applications should be similar to those of CFDA SE (commonly called CFSE). Cells labeled with our SNARF-1 carboxylic acid, acetate, succinimidyl ester have orange-red fluorescence that can be easily distinguished from that of cells loaded with green-fluorescent tracers such as CFDA SE.
Catalog: S22801
Size: 10 × 50 µg
Size: 10 × 50 µg
Zenon® labeling technology provides a fast, versatile, and reliable method for adding a fluorescent label to an antibody. You need only a small amount of starting material,and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Antibody conjugates formed using Zenon® technology may be used in protocols where a directly labeled primary antibody is suitable, including flow cytometry, imaging, and high-throughput applications. This exclusive Molecular Probes® Zenon® labeling technology greatly simplifies the use of multiple mouse-derived...
Catalog: Z11203
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 525 Antibody Labeling Kit. This kit replaces the conventional Qdot® 525 Antibody Conjugation Kit (Q22041MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10449
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 565 Antibody Labeling Kit. This kit replaces the conventional Qdot® 565 Antibody Conjugation Kit (Q22031MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10450
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 585 Antibody Labeling Kit. This kit replaces the conventional Qdot® 585 Antibody Conjugation Kit (Q22011MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10451
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 605 Antibody Labeling Kit. This kit replaces the conventional Qdot® 605 Antibody Conjugation Kit (Q22001MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10469
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ R-PE Antibody Labeling Kit. Unlike conventional antibody labeling kits, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. Depending upon the label, the resulting SiteClick™ labeled antibody can be used in flow cytometry or any other application. Importan...
Catalog: S10467
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 625 Antibody Labeling Kit. This kit replaces the conventional Qdot® 625 Antibody Conjugation Kit (A10197). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction st...
Catalog: S10452
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 655 Antibody Labeling Kit. This kit replaces the conventional Qdot® 655 Antibody Conjugation Kit (Q22021MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction ...
Catalog: S10453
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 705 Antibody Labeling Kit. This kit replaces the conventional Qdot® 705 Antibody Conjugation Kit (Q22061MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction ...
Catalog: S10454
Size: 1 kit
Size: 1 kit
aldehyde-, ketone- & carboxylate-reactive labels
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