Ab Steponeplus™

Isotype Control For Rabbit Primary Ab

Category: Products & Services > Antibodies & Secondary Detection Reagents > Isotype Antibody Controls

Properly controlled IHC stains require that a non-immune immunoglobulin equivalent of the primary antibody be run on the sample tissue in the same way that the primary antibody is run. Any positive staining would indicate that the primary antibody stain may not be specific. These reagents can be used as negative isotype controls for our predilute primary antibodies.
Catalog: 08-6199
Size: 18 ml

AB-Human Serum

Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards

AB-Human Serum (heat-inactivated)
Catalog: 34005-100
Size: 100 ml

AB-TUBE-ANTI--MYC

Category: Products & Services > Slide & Chip Arrays

AB-TUBE-ANTI--MYC
Catalog: 460603
Size: 1 each

Ion Total RNA-Seq Kit for AB Library Builder™ System

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion Total RNA-Seq Kit for AB Library Builder™ System combines optimized reagents and protocols for automated preparation of representative cDNA libraries for strand-specific RNA sequencing of all types of RNA species on the Ion Personal Genome Machine (PGM™) and Proton™ sequencers. Either small RNA (such as microRNA) or whole transcriptome RNA samples can be prepared for next-generation sequencing. The Ion Total RNA-Seq Kit for AB Library Builder™ System contains the same performance advantages as the manual Ion Total RNA-Seq Kit v2 with the convenience of protocol automation. The gel size...
Catalog: 4482416
Size: 13 reactions

Ion Total RNA Library Protocol Card for AB Library Builder™ System

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories

The Ion Total RNA Library Protocol Card for AB Library Builder™System is required for running the Ion Total RNA-Seq Kit for AB for Library Builder™System . This protocol card contains the firmware needed to easily automate the creation of up to 13 whole transcriptome or small RNA (miRNA) libraries upstream of semiconductor sequencing on either the Ion PGM™ or Ion Proton™ platforms. The Ion Total RNA Library Protocol Card for AB Library Builder™System: • Enables whole transcriptome or small RNA (miRNA) library construction on the AB Library Builder™System • Eliminates the need for instrument pr...
Catalog: 4482563
Size: 1 card

TaqMan® Universal PCR Master Mix, No AmpErase® UNG, 2-Pack (2 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4364341
Size: 400 reactions

TaqMan® Universal PCR Master Mix, No AmpErase® UNG, 1 Bulk Pack (1 x 50 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4326614
Size: 2000 reactions

Protein Thermal Shift™ Starter Kit

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Protein Analysis Kits

Applied Biosystems® Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest. Save Time and Money with Your Sample Screening This solution significantly reduces the time and cost associated with screening samples relative to currently available methods. Protein Thermal Shift™ products that are run on our real-time PCR sys...
Catalog: 4462263
Size: 2000 reactions

Primer Express® Software v3.0.1, 5-User License

Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software

The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination. • Enables individual oligonucleotide design for real-time PCR applications. • Supports assays based o...
Catalog: 4363993
Size: 1 license

TaqMan® Universal PCR Master Mix, 1-Pack (1 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Applied Biosystems® TaqMan® Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® real-time PCR instru...
Catalog: 4304437
Size: 200 reactions

Power SYBR® Green PCR Master Mix, 1-Pack (1 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR • The optimized formulation contains highly purified AmpliTaq Gold® D...
Catalog: 4367659
Size: 200 reactions

TaqMan® Fast Universal PCR Master Mix (2X), No AmpErase® UNG, 2-Pack

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Need Results Fast Applied Biosystems® TaqMan® Fast Universal Master Mix (2x), No AmpErase® UNG delivers results in 40 minutes for 40 cycles of PCR in a 20 µL reaction volume using the Applied Biosystems® 7900HT and Fast 7500 Fast Real-Time PCR Systems. • Everything you need for TaqMan®-based qPCR amplification and detection in a convenient single-tube format. TaqMan® Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR • TaqMan® Fast Universal PCR Master Mix (2x), No AmpErase® UNG is compatible with TaqMan® Gene Expressio...
Catalog: 4366072
Size: 500 reactions

MicroAmp® Fast Optical 96-Well Reaction Plate with Barcode, 0.1 mL

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > PCR Plates

The Applied Biosystems® MicroAmp® Fast 96-Well Reaction Plate with Barcode is an integral component of the Applied Biosystems® Fast PCR System, which reduces PCR reaction time from 2 hours to as little as 25 minutes. • Maximum thermal conductivity for precise thermal cycling. • Reproducible, specific and sensitive PCR results in just 25 minutes. • Validated with other system components for consistent results. • Each reaction plate includes a unique serialized, eight character number label (bar code) that is user-readable and machine-readable to prevent tracking errors. Increased Thermal Contac...
Catalog: 4346906
Size: 20 plates

SYBR® Green PCR Master Mix, 1-Pack (1 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial. • Premixed components stored at 2–8°C significantly reduce assay setup time • SYBR® Green I dye detects double-stranded DNA, so specific probes are not required • AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance • dUTP significant...
Catalog: 4309155
Size: 200 reactions

TaqMan® Gold with Buffer A Pack (1000 Reactions)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

This kit contains components needed to perform up to 1000 assays using TaqMan® probes. The kit employs AmpliTaq Gold® DNA polymerase for enhanced performance, 10X TaqMan Buffer A, and magnesium chloride. The components are provided in bulk and enable flexibility in assay setup. Separate Components Provide Flexibility in Assay Set-Up AmpliTaq Gold® DNA polymerase increses yield and gives a more robust 5' nuclease assay than AmpliTaq® DNA polymerase. Passive Reference I in the 10X TaqMan® Buffer A normalizes signal in all TaqMan® Assay reactions and corrects inter-well signal variation caused by...
Catalog: 4304441
Size: 1000 reactions

mir Vana™ qRT-PCR miRNA Detection Kit

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > miRNA qRT-PCR Kits

The mir Vana™ qRT-PCR Detection Kit has been optimized to amplify specific small RNA using qRT-PCR primer sets (not included), enabling accurate quantitation of miRNA expression levels. The novel detection system can be used to characterize miRNA expression via traditional endpoint RT-PCR or real-time RT-PCR using SYBR® Green I. • Quantitative results in less than 3 hours • Detection from as little as 25 pg of total RNA • Suitable for either real-time or end-point RT-PCR • Ideal for validating expression profiling data and high-throughput applications. MicroRNAs (miRNAs) are evolutionarily con...
Catalog: AM1558
Size: 200 reactions

Primer Express® Software v3.0.1, 1-User License

Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software

The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination. • Enables individual oligonucleotide design for real-time PCR applications. • Supports assays based o...
Catalog: 4363991
Size: 1 license

Power SYBR® Green RNA-to-C T ™ 1-Step Kit (Mini-Pack)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qRT-PCR Kits

Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-C T ™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes: • AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA • An additive that red...
Catalog: 4391178
Size: 40 reactions

TaqMan® Gene Expression Master Mix, 10-Pack (10 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Get precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems® TaqMan® Gene Expression Master Mix. TaqMan® Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan® Gene Expression Master Mix has been validated with TaqMan® Gene Express...
Catalog: 4369542
Size: 2000 reactions

TaqMan® Universal Master Mix II, no UNG Mini-Pack (1 x 1 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

TaqMan® Universal Master Mix II is a convenient 2X mix for use in all real-time application including microRNA, gene expression and genotyping. TaqMan® Universal Master Mix II is a convenient 2X mix that includes: -AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure), a highly purified thermostable DNA polymerase, that is hot start-enabled for convenient reaction setup at room temperature -Available in two formats: with and without uracil-DNA glycosylase (UDG) and dUTP⁄dTTP mix to minimize carryover PCR contamination -Optimized salt, dNTP, and buffer concentrations for reliable performance -Passive ...
Catalog: 4440043
Size: 40 reactions