Ab 7500 Standard Mode

7300 & 7500 Real Time PCR Systems TaqMan®RNase P 96-Well Instrument Verification Plate

Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Real-Time PCR Instrument Calibration Kits & Reagents

The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. T...
Catalog: 4350584
Size: 1 kit

TaqMan® GTXpress™ Master Mix, Mini-Pack (1 mL)

Category: Products & Services > Kits & Assays > Genotyping Kits > SNP Genotyping Kits

The TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction). • Compatible with both Fast and Standard mode thermal cycling conditions • Pre-mixed components stored at 2°–8°C significantly reduce assay setup time • Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays •...
Catalog: 4403311
Size: 400 reactions

TaqMan® Universal PCR Master Mix, No AmpErase® UNG, 2-Pack (2 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4364341
Size: 400 reactions

TaqMan® Universal PCR Master Mix, 1-Pack (1 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Applied Biosystems® TaqMan® Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® real-time PCR instru...
Catalog: 4304437
Size: 200 reactions

SYBR® Green PCR Master Mix, 1-Pack (1 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial. • Premixed components stored at 2–8°C significantly reduce assay setup time • SYBR® Green I dye detects double-stranded DNA, so specific probes are not required • AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance • dUTP significant...
Catalog: 4309155
Size: 200 reactions

TaqMan® Gold with Buffer A Pack (1000 Reactions)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

This kit contains components needed to perform up to 1000 assays using TaqMan® probes. The kit employs AmpliTaq Gold® DNA polymerase for enhanced performance, 10X TaqMan Buffer A, and magnesium chloride. The components are provided in bulk and enable flexibility in assay setup. Separate Components Provide Flexibility in Assay Set-Up AmpliTaq Gold® DNA polymerase increses yield and gives a more robust 5' nuclease assay than AmpliTaq® DNA polymerase. Passive Reference I in the 10X TaqMan® Buffer A normalizes signal in all TaqMan® Assay reactions and corrects inter-well signal variation caused by...
Catalog: 4304441
Size: 1000 reactions

TaqMan® Universal PCR Master Mix, No AmpErase® UNG, 1 Bulk Pack (1 x 50 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4326614
Size: 2000 reactions

Power SYBR® Green PCR Master Mix, 1-Pack (1 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR • The optimized formulation contains highly purified AmpliTaq Gold® D...
Catalog: 4367659
Size: 200 reactions

Power SYBR® Green RNA-to-C T ™ 1-Step Kit (Mini-Pack)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qRT-PCR Kits

Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-C T ™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes: • AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA • An additive that red...
Catalog: 4391178
Size: 40 reactions

TaqMan® Gene Expression Master Mix, 10-Pack (10 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Get precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems® TaqMan® Gene Expression Master Mix. TaqMan® Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan® Gene Expression Master Mix has been validated with TaqMan® Gene Express...
Catalog: 4369542
Size: 2000 reactions

TaqMan® Universal Master Mix II, no UNG Mini-Pack (1 x 1 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

TaqMan® Universal Master Mix II is a convenient 2X mix for use in all real-time application including microRNA, gene expression and genotyping. TaqMan® Universal Master Mix II is a convenient 2X mix that includes: -AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure), a highly purified thermostable DNA polymerase, that is hot start-enabled for convenient reaction setup at room temperature -Available in two formats: with and without uracil-DNA glycosylase (UDG) and dUTP⁄dTTP mix to minimize carryover PCR contamination -Optimized salt, dNTP, and buffer concentrations for reliable performance -Passive ...
Catalog: 4440043
Size: 40 reactions

MicroAmp® Adhesive Film Applicator

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Adhesive Film & Other Plate-Related Accessories

Use Applied Biosystems® MicroAmp® Adhesive Film Applicator to form a tight seal between a microplate and an adhesive film. Form a Tight Seal The MicroAmp® Adhesive Film Applicator enables you to ergonomically push an adhesive film down upon a microplate to form a tight seal and minimize air bubbles. This tight seal will prevent well-to-well contamination and sample loss. Quick and Easy Use the MicroAmp® Adhesive Film Applicator to speed the process of sealing multiple microplates. This tool is designed according to the microplate dimensions so you can quickly seal a microplate. The rugged prop...
Catalog: 4333183
Size: 5 applicators

MicroAmp® Optical 96-Well Reaction Plate

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > PCR Plates

The Applied Biosystems® MicroAmp® Optical 96-Well Reaction Plate is optimized to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a ninety-six well format is compatible with Applied Biosystems® 96-Well Real-Time PCR Systems and Thermal Cyclers. • Frosted to minimize interfering fluorescence from wells of cycling block in real-time PCR applications • Screened to eliminate auto-fluorescent plates • Unique design provides a barrier to ambient air to help insure well-to-well temperatur...
Catalog: N8010560
Size: 10 plates

MicroAmp® 96-Well Tray for VeriFlex™ Blocks

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Adhesive Film & Other Plate-Related Accessories

The Applied Biosystems® MicroAmp® 96-Well Tray for VeriFlex™ Blocks is specifically designed to snugly hold MicroAmp® Fast Reaction Tubes with Caps and MicroAmp® Fast 8-Tube Strips (0.1 mL) in a 96-well format. They are also designed to snugly hold MicroAmp® Reaction Tubes with Caps (0.2 mL). Engineered for efficient sample processing Designed specifically for MicroAmp® Fast Reaction Tubes with Caps (0.1 mL) and MicroAmp® Fast 8-Tube Strips (0.1 mL) Designed specifically for MicroAmp® Reaction Tubes with Caps (0.2 mL) Manufactured to high specification, ensuring a quality product Convenience a...
Catalog: 4379983
Size: 10 trays

MicroAmp® Optical Adhesive Film

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Adhesive Film & Other Plate-Related Accessories

The Applied Biosystems® MicroAmp® Optical Adhesive Film reduces the chance of well-to-well contamination and sample evaporation when applied to a microplate. • Pressure-sensitive adhesive provides a tight seal over every well • Will not interfere with sample reading • Press-N-Seal: Align properly to the plate, then press to seal • Easy to apply-will not stick to your glove Prevent Loss of Precious Samples Use this optically-clear adhesive film to seal the samples into the wells of a microplate. This will reduce the possibility of cross-contamination between sample wells and help ensure consist...
Catalog: 4360954
Size: 25 covers

MicroAmp® Optical Film Compression Pad

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Adhesive Film & Other Plate-Related Accessories

Applied Biosystems® MicroAmp® Optical Film Compression Pads are designed to support a proper thermal seal between the thermal cycler and reaction plates when using adhesive films. Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4312639
Size: 5 each

MicroAmp® Optical 96-Well Reaction Plate with Barcode

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > PCR Plates

The Applied Biosystems® MicroAmp® Optical 96-Well Reaction Plate is optimized to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a ninety-six well format is compatible with Applied Biosystems® 96-Well Real-Time PCR Systems and Thermal Cyclers. • Frosted to minimize interfering fluorescence from wells of cycling block • Screened to eliminate auto-fluorescent plates • Unique design provides a barrier to ambient air to help insure well-to-well temperature uniformity • Each reaction p...
Catalog: 4326659
Size: 500 plates

mir Vana™ qRT-PCR miRNA Detection Kit

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > miRNA qRT-PCR Kits

The mir Vana™ qRT-PCR Detection Kit has been optimized to amplify specific small RNA using qRT-PCR primer sets (not included), enabling accurate quantitation of miRNA expression levels. The novel detection system can be used to characterize miRNA expression via traditional endpoint RT-PCR or real-time RT-PCR using SYBR® Green I. • Quantitative results in less than 3 hours • Detection from as little as 25 pg of total RNA • Suitable for either real-time or end-point RT-PCR • Ideal for validating expression profiling data and high-throughput applications. MicroRNAs (miRNAs) are evolutionarily con...
Catalog: AM1558
Size: 200 reactions