Ab 7300 Calibration Sybr Green
Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR • The optimized formulation contains highly purified AmpliTaq Gold® D...
Catalog: 4367659
Size: 200 reactions
Size: 200 reactions
Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial. • Premixed components stored at 2–8°C significantly reduce assay setup time • SYBR® Green I dye detects double-stranded DNA, so specific probes are not required • AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance • dUTP significant...
Catalog: 4309155
Size: 200 reactions
Size: 200 reactions
Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-C T ™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes: • AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA • An additive that red...
Catalog: 4391178
Size: 40 reactions
Size: 40 reactions
This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and ROX™ dyes on the StepOne™ Real-Time PCR System. Features of the StepOne™ Real-Time PCR System Spectral Calibration Kit include: • 48-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, and ROX™) are provided for quick and easy calibration of the StepOne™ Real-Time PCR System • Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture • Includes the Passive Reference I dye standard for sig...
Catalog: 4371433
Size: 1 kit
Size: 1 kit
The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. T...
Catalog: 4350584
Size: 1 kit
Size: 1 kit
This precision plate holder is for use with Applied Biosystems® 7300 real-time PCR systems (SN 273000878 and above), as well as 7500 real-time PCR systems (SN 275001330 and above). Customers with earlier serial numbers should contact Product Support .
Catalog: 4367032
Size: 1 each
Size: 1 each
This single 5-isomer preparation of our proprietary, amine-reactive dye, Oregon Green 488 carboxylic acid, succinimidyl ester and its conjugates have green fluorescence similar to that of fluorescein but are more photostable. Additionally, Oregon Green 488 dye has a lower pK a than fluorescein (pK a = 4.7 versus 6.4 for fluorescein), making this bright dye less pH sensitive in the physiological range.
Catalog: O-6147
Size: 5 mg
Size: 5 mg
Pacific Green™ Succinimidyl Ester is a violet-excitable (411 nm) dye with an emission maximum at ~500 nm, allowing it to be used with other violet-excitable fluorophores (such as Pacific Blue™ or Pacific Orange™ dye) for multiparameter analysis using a single excitation source. As a class of compounds, succinimidyl esters offer an efficient and easy-to-use means for selectively linking a dye to accessible primary amine groups on proteins, modified nucleic acids, or other biomolecules. The covalent bonds formed are as stable as the peptide bonds used to link the amino acids within proteins. Pac...
Catalog: P11202
Size: 250 µg
Size: 250 µg
Zenon® labeling technology provides a fast, versatile, and reliable method for adding a fluorescent label to an antibody. You need only a small amount of starting material,and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Antibody conjugates formed using Zenon® technology may be used in protocols where a directly labeled primary antibody is suitable, including flow cytometry, imaging, and high-throughput applications. This exclusive Molecular Probes® Zenon® labeling technology greatly simplifies the use of multiple mouse-derived...
Catalog: Z11203
Size: 1 kit
Size: 1 kit
Our Streptavidin, Pacific Green™ conjugate, is a Molecular Probes® fluorescent probe that absorbs at at 411 nm and is optimally excited by the violet laser. Streptavidin is a non-glycosylated biotin-binding protein isolated from bacteria with a near-neutral isoelectric point. It is widely used to detect biotinylated probes because it reportedly exhibits less non-specific binding than the glycosylated, biotin-binding protein avidin.
Catalog: S11200
Size: 500 µl
Size: 500 µl
This highly cross-adsorbed Pacific Green™ Goat anti-Mouse IgG is labeled with our violet-excitable Pacific Green™ dye and prepared from affinity-purified antibodies that react with IgG heavy chains and most classes of immunoglobulin light chains from mouse. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG, and human serum. Pacific Green™ dye is a Molecular Probes® fluorophore that absorbs at 411 nm and is optimally excited by the violet laser.
Catalog: P11204
Size: 500 µl
Size: 500 µl
The CD8A, clone 5H10, Pacific Green™ dye conjugate is a monoclonal mouse antibody that is reactive to the mouse CD8α (Ly-2) antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD8α, Rat IgG2b monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species...
Catalog: C11214
Size: 125 µl
Size: 125 µl
The HLA-DR, clone Tü36, Pacific Green™ conjugate is a monoclonal mouse antibody that recognizes the class II human leukocyte (HLA)-DR antigens. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: HLA-DR, Mouse IgG2b monoclonal Label: Pacific Green™ dye Validated Application: Flow Cy...
Catalog: H11212
Size: 125 µl
Size: 125 µl
The CD4, clone RM4-5, Pacific Green™ dye conjugate is a monoclonal rat antibody that recognizes the mouse CD4 antigen and the expressed product of the CD4 gene. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD4, Rat IgG2a monoclonal Label: Pacific Green™ dye Validated Applicat...
Catalog: C11207
Size: 125 µl
Size: 125 µl
The CD8, clone 3B5, Pacific Green™ dye conjugate is a monoclonal mouse antibody that recognizes the CD8 antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD8, Mouse IgG2a monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity: Human ...
Catalog: C11205
Size: 125 µl
Size: 125 µl
The CD45, clone HI30, Pacific Green™ dye conjugate is a monoclonal mouse antibody that is reactive to the human CD45 antigen and recognizes the expressed product of the PTPRC gene. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD45, Mouse IgG1 monoclonal Label: Pacific Green™ ...
Catalog: C11208
Size: 125 µl
Size: 125 µl
The CD3, clone S4.1, Pacific Green™ dye conjugate is a monoclonal antibody that is reactive to the human CD3 antigen.The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD3, Mouse IgG2a monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity: Hu...
Catalog: C11216
Size: 125 µl
Size: 125 µl
The CD3, clone UCHT1, Pacific Green™ dye conjugate is a monoclonal antibody that is reactive to the human CD3 antigen and recognizes the expressed product of the CD3D gene. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD3, Mouse IgG1 monoclonal Label: Pacific Green™ dye Valid...
Catalog: C11213
Size: 125 µl
Size: 125 µl
The CD45R (B220), clone RA3-B62, Pacific Green™ dye conjugate is a monoclonal rat antibody that recognizes the expressed product of the PTPRC gene. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD45R (B220), Rat IgG2a monoclonal Label: Pacific Green™ dye Validated Application:...
Catalog: C11206
Size: 125 µl
Size: 125 µl
The CD45RA, clone MEM-56, Pacific Green™ conjugate is a monoclonal mouse antibody that recognizes the CD45RA antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD45RA, Mouse IgG2b monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity...
Catalog: C11209
Size: 125 µl
Size: 125 µl
Ab 7300 Calibration Sybr Green
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