Nuclease-Free Water
Ambion® Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving. Because it is not DEPC treated, it is recommended for applications for which residual DEPC might prove detrimental (e.g. oocyte microinjection). This nuclease-free water is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
Catalog: AM9930
Size: 1 × 500 ml
Size: 1 × 500 ml
UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-µm membrane-filtered and tested for DNase, RNase, and protease activity.
Catalog: 10977035
Size: 500 ml
Size: 500 ml
Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Catalog: 23600042
Size: 1 x 10L
Size: 1 x 10L
Ambion® RT-PCR grade water is tested for prokaryotic as well as eukaryotic genomic DNA contamination using an ultrasensitive PCR assay. It is provided in 10 x 1.5 mL. Prokaryotic DNA contamination is assessed by performing PCR with primers to 16S rRNA. Eukaryotic DNA contamination is assessed by performing PCR with primers to 18S rRNA. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity and is suitable for any PCR application.
Catalog: AM9935
Size: 10 × 1.5 ml
Size: 10 × 1.5 ml
Gibco® Distilled Water is high quality water for use as a solvent in the preparation of cell culture media and laboratory reagents. Available in many sizes to meet your needs, Gibco® Distilled Water features: • Cell culture quality • Proven performance • Use in cell culture and molecular biology applications Cell culture quality Gibco® Distilled Water is prepared by distillation and filtration (0.1 µm), with no added substances. Life Technologies monitors the water quality on a routine basis. Conductivity, total organic carbon, endotoxin and bioburden are tested daily; metals are tested monthl...
Catalog: 15230-071
Size: 100 ml
Size: 100 ml
Highly purified water (at least ASTM Type III or ISO Grade 3) is required when using the M220 Focused-ultrasonicator™. One AFA-grade water bottle (800 ml) is sufficient for more than 50 uses.
Catalog: 4482280
Size: 1 each
Size: 1 each
Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907
Size: 50 reactions
Size: 50 reactions
S1 Nuclease is a single-strand-specific endonuclease that hydrolyzes single-stranded RNA or DNA into 5´ mononucleotides. The enzyme will hydrolyze single-stranded regions in duplex DNA such as loops and gaps. S1 Nuclease is stable at 65°C. Applications: Nuclease mapping techniques (1,2). Removal of single-stranded regions from double-stranded DNA (3). Exo III-ordered sequencing (4). Source: Isolated from Aspergillus oryza e. Performance and Quality Testing: Double-strand-specific deoxyribonuclease and phosphatase assays. Unit Definition: One unit hydrolyzes 1 µg of denatured DNA to acid-solubl...
Catalog: 18001-016
Size: 20,000 units
Size: 20,000 units
Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K + /H + ionophore nigericin , which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K + .
Catalog: N-1495
Size: 10 mg
Size: 10 mg
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Catalog: 13151-014
Size: 100 ml
Size: 100 ml
High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Catalog: HYL-250
Size: 500 ml
Size: 500 ml
Ambion® Water Saturated Phenol is supplied premixed and at pH 6.6 (pH 7.9 ± 0.2, with included buffer). Water Saturated Phenol is used in RNA and DNA extraction procedures and is provided in one bottle of 400 mL. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, and easier alternative to preparing solutions from crystalline phenol.
Catalog: AM9712
Size: 400 ml
Size: 400 ml
The RNase-free TE buffer concentrate, 20X TE buffer , which is essential to the success of the RiboGreen® RNA quantitation assays (R11491, R37302) is available separately from the kits to extend the number of low-concentration assays.
Catalog: T11493
Size: 100 ml
Size: 100 ml
Ambion® RNase-free Microfuge Tubes are guaranteed RNase- and DNase-free and have a positive seal design that withstands –90° to 120°C. They are marked with convenient graduations. 1.5 mL tubes are provided in a box of 500. Each lot of tubes is certified RNAse- and DNase-free.
Catalog: AM12400
Size: 500 tubes
Size: 500 tubes
Ambion® IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage. IPTG is also used to induce the expression of cloned genes that are under control of the lac operon. Supplied in one bottle containing 5 g.
Catalog: AM9464
Size: 5 g
Size: 5 g
Propidium iodide is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.
Catalog: P3566
Size: 10 ml
Size: 10 ml
Ambion® Non-Stick RNase-free Microfuge Tubes have a low binding surface and are guaranteed RNase- and DNase-free. They have a positive seal design that withstands –90° to 120°C and are marked with convenient graduations. 1.5 mL tubes are provided in a box of 250. Each lot of tubes is certified RNAse- and DNase-free.
Catalog: AM12450
Size: 250 tubes
Size: 250 tubes
Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907M
Size: 50 rxns w⁄ Manual
Size: 50 rxns w⁄ Manual
Human Endothelial-SFM is a basal serum-free growth medium developed to support the isolation and expansion of human umbilical venous and arterial endothelial cells (1–4). Medium requires supplementation with bFGF (20 ng/ml), EGF (10 ng/ml), and human plasma fibronectin (10 µg/ml). Contains L-glutamine.
Catalog: 11111-044
Size: 500 ml
Size: 500 ml
Ambion® RNaseZap® is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® onto the surface to be decontaminated and rinse it off with RNase-free water. Supplied in a single 4 liter cubitainer. • Completely removes RNase contamination from glass and plastic surfaces • Excels at removing high levels of RNase contamination whereas similar products fail • Proven effective at removing high concentrations of dried-on RNase A • Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free Working with RNA requires that special measures be...
Catalog: AM9784
Size: 4 L
Size: 4 L
Nuclease-Free Water
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