2x Samples Buffer for Protein Gels

Novex® Tris-Glycine SDS Sample Buffer (2X)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers

Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2676
Size: 20 ml

Novex® TBE-Urea Sample Buffer (2X)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

Denaturing polyacrylamide TBE-Urea Gels resolve single-stranded DNA oligos or RNA into sharp, distinct bands. Novex® TBE-Urea Gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and Northern blot analysis. Novex® TBE-Urea Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE-Urea Gels are made with high-purity reagents, including tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEME...
Catalog: LC6876
Size: 10 ml

NuPAGE® LDS Sample Buffer (4X)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers

NuPAGE® LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with NuPAGE® Novex® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample Buffer is a hig...
Catalog: NP0007
Size: 10 ml

NuPAGE® LDS Sample Buffer (4X) (250 ml)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers

NuPAGE® LDS Sample Buffer (4X) (250 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sampl...
Catalog: NP0008
Size: 250 ml

NativePAGE™ 5% G-250 Sample Additive

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers

NativePAGE™ 5% G-250 Sample Additive is added to samples prepared with the NativePAGE™ Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE™ gels. This Additive is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.
Catalog: BN2004
Size: 0.5 ml

NuPAGE® Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers

NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. NuPAGE® Novex Tris-Acetate Midi-Gels (8 x 13 cm) can run up to 26 samples. The NuPAGE Midi-Gels run in the XCell4 Surelock™ Midi-Cell and, with a Midi Gel Adapter, can run in a Bio-Rad Criterion Cell. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity,...
Catalog: LA0050
Size: 1 kit