10x Loading Buffer

10X BlueJuice™ Gel Loading Buffer

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

BlueJuice™ Gel Loading Buffer is designed for easy loading and tracking of DNA samples in agarose or native polyacrylamide gels.
Catalog: 10816-015
Size: 3 × 1 ml

Gel Loading Buffer II (Denaturing PAGE)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue. Supplied in one 1.4 mL tube. All Ambion® Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. These are the same solutions we use in-house and in our kits.
Catalog: AM8546G
Size: 1 ml

TrackIt™ Cyan/Orange Loading Buffer

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

The TrackIt™ Loading Buffers are a 6X solution used for easy loading and tracking of DNA samples in agarose gels. These loading buffers contain two tracking dyes, one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers for following migration during electrophoresis and indicate when maximum resolution of the DNA fragments has been achieved. Additionally, visualization of DNA bands will not be obscured by the tracking dyes because they run outside the limits of most DNA samples. TrackIt™ Cyan/Orange Loading Buffer is recommended for DNA f...
Catalog: 10482-028
Size: 3 × 0.5 ml

10X RT Buffer

Category: Products & Services > Buffers & Chemicals

Catalog: AM8703G
Size: 200 µl

Novex® TBE-Urea Sample Buffer (2X)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

Denaturing polyacrylamide TBE-Urea Gels resolve single-stranded DNA oligos or RNA into sharp, distinct bands. Novex® TBE-Urea Gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and Northern blot analysis. Novex® TBE-Urea Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE-Urea Gels are made with high-purity reagents, including tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEME...
Catalog: LC6876
Size: 10 ml

Novex® Hi-Density TBE Sample Buffer (5X)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Catalog: LC6678
Size: 10 ml

PBS - Phosphate-Buffered Saline 10X, pH 7.4

Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS

Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Catalog: AM9624
Size: 500 ml

Novex® Tris-Glycine SDS Running Buffer (10X)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers

Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2675-5
Size: 5 L

GeneAmp® 10X PCR Gold Buffer & MgCl2, 6 Sets (6 x 1.5 mL)

Category: Products & Services > Buffers & Chemicals > Molecular Biology Enzyme Buffers > PCR Enzyme Buffers

Applied Biosystems® GeneAmp®10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold® DNA Polymerase, resulting in highly specific, robust PCR amplification. When diluted 1:10, provides the optimal pH and ionic strength for PCR activation and amplification with AmpliTaq Gold® DNA Polymerase, ensuring maximum performance Available in several convenient sizes GeneAmp® 10X PCR Buffer II is both magnesium ion free and gelatin free. A separate MgCl 2 solution is provided. When used together, GeneAmp® 10X PCR Buffer II and MgCl 2 solution offer more versatility in...
Catalog: 4306898
Size: 6 × 1.5 ml

PBS - Phosphate-Buffered Saline 10X, pH 7.4

Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS

Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminatin...
Catalog: AM9625
Size: 1 L

10X TBE (1 L bottle)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Running Buffers

Molecular biology grade, Ambion® 10X TBE solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating...
Catalog: AM9865
Size: 4 bottle

NuPAGE® Sample Reducing Agent (10X) (10 ml)

Category: Products & Services > Buffers & Chemicals > Reducing Agents

NuPAGE® Reducing Agent (10X) (10 ml) is used to reduce protein samples for protein gel electrophoresis. NuPAGE® Reducing Agent contains 500 mM dithiothreitol (DTT) at a ready-to-use 10X concentration in a stabilized liquid form. Use NuPAGE® Sample Reducing Agent when running proteins on NuPAGE® Bis-Tris gels to maintain protein samples in a reduced state during electrophoresis. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
Catalog: NP0009
Size: 10 ml

NorthernMax®-Gly 10X Gel Prep/Running Buffer

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Northern Blotting Kits & Reagents

For the preparation and running of agarose gels. For use with RNA denatured by glyoxal treatment; one bottle containing 1 L is provided. Ambion® NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.
Catalog: AM8678
Size: 1 L

NorthernMax® 10X Denaturing Gel Buffer

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Northern Blotting Kits & Reagents

An improved formula for preparing denaturing formaldehyde/MOPS agarose gels. Formaldehyde is included. Gels made with this buffer are considerably less brittle than conventional formaldehyde gels, making handling of the gels much easier. One bottle containing 250 mL is provided. NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.
Catalog: AM8676
Size: 250 ml