Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Modifying Enzymes
DNA End Repair Mix offers an easy, one step process for creating blunt ends in sheared, sonicated, or restriction-cut DNA and PCR fragments for efficient ligation into blunt end cloning vectors. DNA End Repair Mix allows you to: Generate blunt ends in DNA and PCR fragments (A-tail) Repair DNA with 5′ or 3′ overhangs in 30 minutes at 37°C Heat inactivate DNA End Repair Mix in 10 minutes at 75°C A Simple Way to Make Blunt Ends With DNA End Repair Mix, DNA with 5′ or 3′ overhangs is converted to 5′ phosphorylated blunt end DNA in 30 minutes at 37°C. Once DNA has been repaired, the enzymes in DNA ...
Catalog: A14321
Size: 20 reactions
Category: Products & Services > Buffers & Chemicals > Detergents & Solubilizers
This reagent, component the Fetal Hemoglobin (HbF) testing, is a 0.1% working solution is prepared in phosphate-buffered saline (PBS) containing bovine serum albumin (BSA) and sodium azide. Class: GPR Statement: This product is for GPR Use Preservative: 0.1% Sodium Azide Stabilizer: 0.1% BSA
Catalog: HFH-10
Size: 10 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents
Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types. With Lipofectamine® RNAiMAX Transfection Reagent you will get: Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects Easy optimization due to minimal cytotoxicity across a 10-f...
Catalog: 13778-100
Size: 0.1 ml
Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals
Ambion® Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for cont...
Catalog: AM9260G
Size: 100 ml
Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells
One Shot® TOP10 E. coli are convenient to use chemically competent cells quality controlled for a transformation efficiency of 1 x 10 9 cfu/ µg plasmid DNA. One Shot® TOP10 Chemically Competent cells are the same competent cells that come with many of our cloning kits and are ideal for high-efficiency cloning and plasmid propagation. • Maximizes cloning efficiency in single tube format • Enhanced genomic DNA cloning capabilities Easy-to-use, Single Tube Format One Shot® TOP10 E. coli are provided at a transformation efficiency of 1 x 10 9 cfu/ µg supercoiled DNA and allow stable replication of...
Catalog: C4040-06
Size: 40 reactions
Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
Invitrogen Cal-Lyse™ is a lysing solution formulated to enable the lysis of erythrocytes in samples of anticoagulated human peripheral blood. Invitrogen Cal-Lyse™ lysing solution is intended to be used as an aid in the enumeration of leukocytes that have been stained with monoclonal antibodies for flow cytometric analysis
Catalog: GAS-010S-100
Size: 100 ml
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers
The Magnetic 96-Well Separator is designed for fast and simple removal of supernatant from samples bound to magnetic particles and may be used manually or as an integrated part of an automated workstation. The Magnetic 96-Well Separator is an ideal tool for use in singleplex and multiplex biological assays based on the Luminex® xMAP® Technology. These assays make use of Luminex® MagPlex™, or magnetic microspheres, and the MAGPIX®, Luminex® 200™ or FLEXMAP 3D® instruments. Note: It is recommended that the Magnetic 96-Well Separator be used with a standard 96-well flat-bottom plate such as Grein...
Catalog: A14179
Size: 1 each
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Human PGK1 (phosphoglycerate kinase 1) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: PG...
Catalog: 4326318E
Size: 1000 reactions
Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Other Enzyme Assay Kits
The NA-Fluor™ Influenza Neuraminidase Assay Kit provides validated reagents and a standardized protocol for conducting neuraminidase enzyme assays, including neuraminidase inhibitor (NI) susceptibility screening using a fluorescent MUNANA substrate. Key product features: • Efficient design—neuraminidase assay in one complete kit • Optimized formulations—assay reagents have been optimized for maximum performance based on NISN protocols • Robust application—functional detection of NI-resistant virus in mixed viral populations • Easy of use & flexible screening—stable fluorescent signal enables b...
Catalog: 4457091
Size: 960 assays
Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > DNA & RNA Storage Reagents
RNAlater®-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater®-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA. • Process previously frozen tissues like freshly harvested samples • Thawing tissues in RNAlater®-ICE protects RNA from degradation • No more tissue pulverizing with mortar and pestle and awkward transf...
Catalog: 4427575
Size: 10 bottles
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose
UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Low Melting Point Agarose is ideal for resolving DNA and RNA fragments larger than 1,000 bp and for the recovery of nucleic acid fragments after electrophoresis, as it melts at 65.5°C (below the melting point of most nucleic acids). The solution remains fluid at 37°C and will set rapidly at temperatures below 25°C. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handlin...
Catalog: 16520100
Size: 100 g
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Counting, & Flow Cytometer Accessories
This is a cold block for use with the ARCTURUS® HistoGene® LCM Immunofluorescence Staining Kit. For Research Use Only. Not for use in diagnostics procedures.
Catalog: HIS0101
Size: 1 each
Category: Products & Services > Kits & Assays > Cell Analysis Kits > HCS Detection Kits
The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid sta...
Catalog: H34476
Size: 1 each
Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains
The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Catalog: E34250
Size: 100 µg
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores
The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (K d ) of fluorescent Ca 2+ indicators using a fluorometer or quantitative imaging system. The Kd of an indicator can be calculated from a plot generated by scanning the excitation or emission of the indicator in the presence of eleven different Ca 2+ concentrations. Calcium Calibration Kit #1 contains 50 mL of 10 mM K 2 EGTA and 50 mL of 10 mM CaEGTA. Both solutions contain 100 mM KCl and 30 mM MOPS, pH 7.2 and are prepared in deionized water. These stock solutions can be blended to make buffers havin...
Catalog: C-3008MP
Size: 1 kit
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin
Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing This Trypsin is modified as follows: With • EDTA • Phenol Red
Catalog: 25300-120
Size: 20 x 100 ml
Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals
Ambion® Water Saturated Phenol is supplied premixed and at pH 6.6 (pH 7.9 ± 0.2, with included buffer). Water Saturated Phenol is used in RNA and DNA extraction procedures and is provided in one bottle of 400 mL. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, and easier alternative to preparing solutions from crystalline phenol.
Catalog: AM9712
Size: 400 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
A complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. • Simple—A single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein • Productive—Obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods • Versatile—Works with many sample sources, including human, plant, yeast, bacterial, and viral samples • Flexible—Easily scalable depending on starting material and desired yield • Now incorporated into our RiboPure™ and MagMAX™-96 for Microarrays RNA Is...
Catalog: AM9738
Size: 100 ml
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Polyacrylamide Gels
Denaturing polyacrylamide TBE-Urea Gels resolve single-stranded DNA oligos or RNA into sharp, distinct bands. Novex® TBE-Urea Gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and Northern blot analysis. Novex® TBE-Urea Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE-Urea Gels are made with high-purity reagents, including tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEME...
Catalog: EC6865BOX
Size: 1 box
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Mouse IgM Specificity: Mouse IgM Reactivity: Mouse Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Catalog: 62-6820
Size: 1 ml
Category: Products & Services > Buffers & Chemicals > Nucleic Acid Reagents
Yeast tRNA is suitable for use as a carrier in nucleic acid purification and precipitation procedures. It is supplied in lyophilized form.
Catalog: 15401-011
Size: 25 mg
Category: Products & Services > Buffers & Chemicals > General Buffers > Other General Buffers
This reagent is intended for the preparation of positive and negative controls from cord blood for Fetal Hemoglobin (HbF) testing. Class: GPR Statement: This product is for GPR use. Preservative: None
Catalog: HFH-11
Size: 100 ml
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > dNTPs
GeneAmp® dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. GeneAmp® dNTPs with dUTP can be used to generate dU-containing PCR products in a carry-over prevention strategy in conjunction with AmpErase ® Uracil N-glycosylase (UNG). dNTPs have high purity, making them ideal for use in PCR reactions or other applications. dNTPs with dUTP are optimized for use in carry-over prevention. Guaranteed for PCR performance when used with GeneAmp® PCR core reagents and GeneAmp® instrument systems. Premixed dNTP blends are available in various conce...
Catalog: 4303443
Size: 12 x 1 ml
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
The Vivid Colors™ Fluorescent Protein Gateway® Destination Vectors allow you to quickly and easily fuse a protein of interest to the widely used and well-characterized fluorescent proteins from the jellyfish Aequorea victoria (1,2) using the pcDNA™ 6.2 Gateway™ mammalian expression vector. These powerful Gateway® Technology vectors contain the next-generation EGFP, Emerald Green Fluorescent Protein (EmGFP), or the popular Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein. Both fluorescent proteins have been humanized for optimal mammalian expression (3)...
Catalog: V355-20
Size: 6 µg
Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Blotting Systems
The XCell line of mini-cells has always distinguished itself by being simple, sturdy, and convenient. The tradition continues with the patented XCell SureLock ® Mini-Cell. The incorporation of a gel tension wedge in place of the rear wedge used on earlier models makes the XCell SureLock ® the easiest-to-use mini-cell on the market. How the System Works The XCell SureLock ® Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even horizontal force is generated. This seals the gel/buffer core assembly ...
Catalog: EI0002
Size: 1 unit
Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Blotting Systems
The XCell II™ Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. It fits neatly into the XCell SureLock ™ and XCell II™ Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 ml of transfer buffer for western, Southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9 cm x 9 cm.
Catalog: EI9051
Size: 1 unit
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The TaqMan® DNA Template Reagents provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells. TaqMan® beta-actin Template Reagents can be used with TaqMan® PCR Core Reagents or SYBR® Green PCR Core Reagents for polymerase chain reaction (PCR). • Pre-designed primers and TaqMan® probe eliminate assay design. • Rapid assay development guidelines can minimize optimization time. • Separate components provide flexibility in assay set-up. Comprehensive Guidelines Included Life Technologies® has developed a comprehensive set of ...
Catalog: 401970
Size: 1 kit
Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
The Protein Quant Sample Lysis Kit contains the reagents required for the preparation of target and control cell lysates for use with TaqMan® Protein Assay experiments. It contains a one-step lysis solution for preparing crude cell lysates from cultured cells, and the lysates are compatible with cell counting as well as total protein quant measurements such as a microBCA assay. The Protein Quant Sample Lysis Kit contains a buffered nonionic detergent solution for the gentle lysis of adherent and non-adherent cultured cells under non-denaturing conditions
Catalog: 4448536
Size: 25 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Stem Cell Media
StemPro®-34 SFM is a serum-free medium specifically formulated to support the development of human hematopoietic cells in culture (1). StemPro®-34 SFM can be used to study hematopoietic cells from bone marrow, peripheral blood, or neonatal cord blood. StemPro®-34 SFM is performance-tested on normal human bone marrow. It is manufactured with components of human, recombinant or synthetic origin. StemPro®-34 SFM consists of a basal liquid medium and frozen supplement (StemPro®-Nutrient Supplement) which is added at the time of use.
Catalog: 10639-011
Size: 500 ml
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2925
Size: 1 mg
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin
Gibco® Trypsin 1:250 powder is an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging, and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing Quality Testing Gibco® Trypsin 1:250 is tested for endotoxin, mycoplasma, bacterial, fungal and viral contamination. Other quality tests include multiple activity assays, ash analysis and mois...
Catalog: 27250-018
Size: 100 g
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
The superoxide indicator dihydroethdium , also called hydroethidine, exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red.
Catalog: D-23107
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The rabbit anti—goat IgG, horseradish peroxidase (HRP) conjugate has been adsorbed against human and rat serum proteins prior to labeling to minimize crossreactivity.
Catalog: R-21459
Size: 1 mg
Category: Products & Services > Cell Culture & Transfection Reagents > Serum > Specialty Fetal Bovine Serum
Sera Category: Specialty Origin: United States. Endotoxin level: 50 EU/ml (Specification is checked and recorded; levels routinely Hemoglobin level: 25 mg/dl.
Catalog: 26400-036
Size: 100 ml
Category: Products & Services > Buffers & Chemicals > Microscopy Reagents
This kit is used to block endogenous biotin or biotin-binding activity in tissue sections. The kit contains 18 mL each of Avidin and Biotin solutions.
Catalog: 00-4303
Size: 1 kit
Category: Products & Services > Eukaryotic Cells > Cell Lines > Mammalian Cell Lines
T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄ lac Z. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).
Catalog: R710-07
Size: 3 × 10^6 cells
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Laser Capture Microdissection (LCM) Kits
The ARCTURUS® HistoGene® LCM Immunofluorescence Staining Kit is designed to retrieve high-quality nucleic acids from immunofluorescently-stained, frozen tissue using a quick 15-minute process. The kit provides reagents and slides for convenient and reliable immunoflourescent staining as well as protocols that are streamlined and optimized to maintain nucleic acid quality. Key product features are: • Simplified staining—retrieve high-quality RNA from simplified fluorescent target cell labeling • Efficient preservation—quick 15-minute process preserves mRNA • Cold sample protection—chill samples...
Catalog: KIT0420
Size: 32 applications
Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
The same lysis buffer found in the Ambion® Cells-to-Signal™ Kit. Supplied in 5 bottles containing 10 mL each.
Catalog: AM8728
Size: 5 bottle
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Insect & Baculovirus Expression Vectors
The DES® TOPO® TA Expression Kit offers one-step cloning of PCR products directly into an inducible DES® expression vector. The kit uses the linearized, topoisomerase I-activated pMT/V5-His-TOPO® vector for fast and easy cloning and subsequent high-level expression. Topoisomerase activation of this vector allows PCR products to be ligated in just 5 minutes on your bench top to produce >85%recombinants. The pMT/V5-His-TOPO® vector has all of the features of the pMT/V5-His vector to simplify protein expression, detection, and purification of recombinant proteins in Drosophila S2 cells.
Catalog: K4125-01
Size: 20 reactions
Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents
Alexa Fluor® 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 647 dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 650/668 nm Superior to antibody staining Optimal for fixed and permeabilized samples Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has...
Catalog: A22287
Size: 300 units
Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents
Lipofectin® Transfection Reagent is the reagent of choice for transfectin of endothelial cells; some of our customer also use it for transfection of DNA, RNA or oligonucleotides into their cell line of choice. Lipofectin® has also been shown to work well in combination with PLUS® reagent for the transfection of HeLa cells (1).
Catalog: 18292-011
Size: 1 ml
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human p53 Signaling 96-well Plate contains 92 assays to p53 signaling associated genes and 4 assays to candidate endogenous control genes.
Catalog: 4418813
Size: 1 Fast plate
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Epitope Tag & Reporter Specific Antibodies
This purified Mouse Histidine (C-term) Tag Monoclonal Antibody recognizes a polyhistidine amino acid sequence at the carboxy-terminus of proteins. The free carboxy-terminus of the terminal histidine residue is a key element of the epitope recognition site of the anti-His (C-term) Antibody. The Anti-His(C-term) Monoclonal Antibody recognizes the sequence: -His-His-His-His-His-His-COOH . Validated applications for Histidine (C-term) Tag monoclonal antibody are western blotting and ELISA. Antibody Specifications: • Applications: Validated applications for Histidine (C-term) Tag Monoclonal Antibod...
Catalog: R931-25
Size: 50 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
Alexa Fluor® 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western ...
Catalog: S-21374
Size: 1 mg
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > 2-D Gels
NuPAGE® Novex Bis-Tris Gels provide the best separation and resolution of small- to medium-sized proteins by utilizing a neutral pH environment which minimizes protein modifications. These high-performance gels have a one-year shelf life when stored at +4°C to 25°C, eliminating the waste of throwing out expired gels. Use NuPAGE® Novex Bis-Tris Gels for protein sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. The NuPAGE® System also provides the most efficient means for transferring proteins to membranes for subsequent analysis. Formulation: The NuPAGE...
Catalog: NP0330BOX
Size: 1 box
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Affinity purified whole antibody goat anti-mouse IgG conjugated to Cy5®. To minimize cross-reactivity, this antibody has been absorbed against human IgG and human serum.
Catalog: A10524
Size: 0.5 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Sheep IgG (H+L) Specificity: Sheep IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Catalog: 61-8600
Size: 2 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers
Denaturing polyacrylamide TBE-Urea Gels resolve single-stranded DNA oligos or RNA into sharp, distinct bands. Novex® TBE-Urea Gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and Northern blot analysis. Novex® TBE-Urea Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE-Urea Gels are made with high-purity reagents, including tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEME...
Catalog: LC6876
Size: 10 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 11058-021
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology. i Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero. This Advanced DMEM is manufactured as follows: With Without • High glucose • L-glutamine • Non Essential Amino Acids • Sodium pyruvate • Phenol Red
Catalog: 12491-015
Size: 500 ml