Products from Nupage® Mops Sds Running Buffer (20x) to Ultrapure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, V/V)

NuPAGE® MOPS SDS Running Buffer (20X)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers

NuPAGE® MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels only. NuPAGE® MOPS SDS Running Buffer is recommended for separating small- to medium-sized proteins. Use the right buffer to optimize protein separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with NuPAGE® Novex® Bis-Tris gels. Use of MOPS buffer allows proteins to run slower than when using MES buffer. Get consistent, convenient, high-quality results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresi...
Catalog: NP0001-02
Size: 5 L

Bovine Albumin Fraction V Solution (7.5%)

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > BSA & Albumin Supplements

7.5 g/100 ml Prepared with Bovine Serum Albumin Fraction V in phosphate-buffered saline.
Catalog: 15260-037
Size: 100 ml

Custom siRNA

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

Ambion® Custom In Vivo Ready synthesized siRNA, 10 µmol. These siRNAs are subjected to that extra level of purification and testing required for the introduction of high quality siRNAs into animals.
Catalog: AM16233
Size: 10 µmol

DynaMag™-5 Magnet

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers

The DynaMag™-5 Magnet is intended for use with Dynabeads® for the immunomagnetic separation of cells. The DynaMag™-5 magnet accommodates tubes from 11–16 mm in diameter (e.g., flow cytometry tubes and most blood collection tubes), with an optimal working volume of 1–5 mL. The magnet holds 8 tubes and is compatible with all Dynabeads® (1–4.5 µM in diameter). When performing cell separation using Dynabeads®, a DynaMag™ magnet is required. Different magnets are optimized for different working volumes and tube sizes. See our complete overview of the DynaMag™ magnets . The DynaMag™-5 magnet contain...
Catalog: 12303D
Size: 8 samples

mir Vana™ qRT-PCR miRNA Detection Kit

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > miRNA qRT-PCR Kits

The mir Vana™ qRT-PCR Detection Kit has been optimized to amplify specific small RNA using qRT-PCR primer sets (not included), enabling accurate quantitation of miRNA expression levels. The novel detection system can be used to characterize miRNA expression via traditional endpoint RT-PCR or real-time RT-PCR using SYBR® Green I. • Quantitative results in less than 3 hours • Detection from as little as 25 pg of total RNA • Suitable for either real-time or end-point RT-PCR • Ideal for validating expression profiling data and high-throughput applications. MicroRNAs (miRNAs) are evolutionarily con...
Catalog: AM1558
Size: 200 reactions

Geneticin® Selective Antibiotic (G418 Sulfate), Powder

Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics

Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a powder and should be m...
Catalog: 11811-031
Size: 5 g

Silencer® Select Pre-Designed siRNA

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

Ambion® Silencer ® Select siRNAs are the result of recent improvements in siRNA design combined with novel chemical modifications yielding unrivaled knockdown efficiency and specificity. Our best performing siRNA for cell culture work. Silencer® siRNAs are unmodified 21 nucleotide siRNAs that have been designed with an older, yet rigorously tested design algorithm. A lower cost alternative to Silencer® Select siRNAs, but without some of the performance enhancements.
Catalog: 4390771
Size: 5 nmol

LysoTracker® Red DND-99 - Special Packaging

Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains

LysoTracker® Red DND-99 is a red-fluorescent dye for labeling and tracking acidic organelles in live cells. Features of all LysoTracker® probes include: • High selectivity for acidic organelles • Good retention after aldehyde fixation • Effective labeling of live cells at nanomolar concentrations • Live cell imaging • Excitation/Emission: 577/590 nm Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate ...
Catalog: L-7528
Size: 20 × 50 µl

CM-H2DCFDA (General Oxidative Stress Indicator)

Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Reactive Oxygen & Nitrogen Species

CM-H 2 DCFDA is a chloromethyl derivative of H 2 DCFDA, useful as an indicator for reactive oxygen species (ROS) in cells. This indicator exhibits much better retention in live cells than H 2 DCFDA. CM-H 2 DCFDA passively diffuses into cells, where its acetate groups are cleaved by intracellular esterases and its thiol-reactive chloromethyl group reacts with intracellular glutathione and other thiols. Subsequent oxidation yields a fluorescent adduct that is trapped inside the cell, thus facilitating long-term studies. ROS Indicator Specifications: • Ex/Em: ~492–495/517–527 nm • Product is air ...
Catalog: C6827
Size: 20 × 50 µg

4-Acetamido-4'-Maleimidylstilbene-2,2'-Disulfonic Acid, Disodium Salt

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels

Our 4-acetamido-4'-maleimidylstilbene-2,2'- disulfonic acid is a thiol-reactive reagent that is water soluble, with high polarity and membrane impermeability. This polysulfonated dye is useful for determining whether thiol-containing proteins and polypeptide chains are exposed at the extracellular or cytoplasmic membrane surface.
Catalog: A-485
Size: 25 mg

Isotype Control For Rabbit Primary Ab

Category: Products & Services > Antibodies & Secondary Detection Reagents > Isotype Antibody Controls

Properly controlled IHC stains require that a non-immune immunoglobulin equivalent of the primary antibody be run on the sample tissue in the same way that the primary antibody is run. Any positive staining would indicate that the primary antibody stain may not be specific. These reagents can be used as negative isotype controls for our predilute primary antibodies.
Catalog: 08-6199
Size: 18 ml

pcDNA™3.1 Directional TOPO® Expression Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The pcDNA™3.1 Directional TOPO® Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® for five-minute directional cloning and subsequent high-level expression. Directional Cloning technology facilitates expression experiments because: A proofreading enzyme is used for fewer errors in cloned genes Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation In addition, pcDNA3.1D/V5-His-TOPO® provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion t...
Catalog: K4900-40
Size: 40 reactions

OWL Centipede™ Gel System

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Boxes

• For use with SSP products or any application requiring agarose gel separation • Multiple formats available to meet various throughput needs • Includes replacement parts for each unit
Catalog: 80000-1D
Size: 1 each

pENTR™/SD/D-TOPO® Cloning Kit with One Shot® TOP10 Chemically Competent E. coli

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Gateway® Entry Vectors - pENTR

The pENTR™/SD/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally into the pENTR™/SD/D-TOPO® entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes. The kit comes with everything necessary to clone and select your PCR amplified gene of interest. Gateway® System Ready - Rapidly shuttle cloned genes between multiple ve...
Catalog: K2420-20
Size: 20 reactions

PARIS™ Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid & Protein Isolation Kits

The Ambion® PARIS™ system is for the isolation of both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The kit contains sufficient reagents for 50 purifications from1 to 75 mg of tissue or 100 to 10 7 cells each. • Fast, simple procedure • Isolate nuclear and cytoplasmic RNA and protein from cultured cells • Isolate total RNA and protein from cultured cells or tissues • No phenol extraction or alcohol precipitation • Reduces time, cost, and variability between independent exper...
Catalog: AM1921
Size: 50 purifications

MitoTracker® Green FM - Special Packaging

Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains

MitoTracker Green FM is green-fluorescent mitochondrial stain which appears to localize to mitochondria regardless of mitochondrial membrane potential. The dye will stain live cells but is not well-retained after aldehyde fixation.
Catalog: M-7514
Size: 20 × 50 µg

Nile Red

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments (excitation/emission maxima ~552/636 nm in methanol).
Catalog: N-1142
Size: 25 mg

AmpliTaq® DNA Polymerase with Buffer I

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits

AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus ( Taq ) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. Features of this enzyme: AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy Its thermal activity profile makes it ...
Catalog: N8080160
Size: 250 units

Luria Broth Base (Miller's LB Broth Base)®, Powder

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media

Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 10 g sodium chloride
Catalog: 12795-027
Size: 500 g

Ambion® RNA-Seq Library Construction Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII is a single kit for RNA library construction of either whole transcriptome or small RNA (such as miRNA), for use in downstream next generation sequencing analysis. Designed as a complete solution with a common workflow, the kit optimally combines all necessary reagents for discovery of coding RNA, non-coding RNA, alternative splicing, small RNAs and isoforms, while preserving strand orientation. The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII platform is based on the same technology used in ...
Catalog: 4454073
Size: 1 kit

LIVE/DEAD® Fixable Blue Dead Cell Stain Kit, for UV excitation

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Viability Kits

The LIVE/DEAD® Fixable Dead Cell Stain Kit employs an amine-reactive fluorescent dye to evaluate mammalian cell viability by flow cytometry. In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity between the live and dead cell populations is typically greater than 50 fold, and this fluorescence intensity discrimination is completely preserved fol...
Catalog: L-23105
Size: 1 kit

MAX Efficiency® Stbl2™ Competent Cells

Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells

MAX Efficiency® Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to rec A1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences (1) and tandem array genes (2). MAX Efficiency® Stbl2™ Competent Cells offer: >1 x 10 9 transformants/flg for cloning rare sequences Elimination of mcr BC- hsd RMS- mrr and mutation of mcr A allows cloning of methylated genomic sequences (1) Unique genotype stabilizes direct repeat and retroviral sequences such as SIV and HIV (1) Greatly ...
Catalog: 10268-019
Size: 1 ml

3 M Sodium Acetate, pH 5.5

Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals

Ambion® Molecular biology grade, 3 M Sodium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for cont...
Catalog: AM9740
Size: 100 ml

Keratinocyte-SFM Medium (Kit) with L-Glutamine, EGF and BPE

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Keratinocyte-SFM

with L-Glutamine, EGF and BPE. Shelf life after supplementation: 2 weeks
Catalog: 17005-075
Size: 1 kit

2-Mercaptoethanol, 50 mM

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > 2-Mercaptoethanol

Gibco® 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. Mercaptoethanol is not stable in solution so most protocols require daily supplementation. Gibco® 2-Mercaptoethanol is made at 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). Product Use For Research Use Only. Not for use in diagnostic procedures. cGMP Manufacturing and Quality System Gibco® 2-Mercaptoethanol is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the...
Catalog: 31350-010
Size: 20 ml

Custom Plus TaqMan® RNA Assay, MED

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

Custom Plus TaqMan® RNA Assay, MED
Catalog: 4441117
Size: 750 µl

Geneticin® Selective Antibiotic (G418 Sulfate), 50 mg/ml Solution

Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics

Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a 50 mg / ml solution in...
Catalog: 10131-027
Size: 100 ml

CellTracker™ Green CMFDA (5-Chloromethylfluorescein Diacetate) - Special Packaging

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents

Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C7025
Size: 20 × 50 µg

Goat Anti Human IgG HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Goat whole molecule anti-human immunoglobulin Format/Formulation: HRP Conjugate
Catalog: AHI0304
Size: 0.7 mg⁄ml

SDS, 20% Solution

Category: Products & Services > Buffers & Chemicals > Denaturing Agents

Ambion® molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL. The buffer is certified RNase-free, economical, and ready-to-use. SDS is used as a component of both hybridization and wash buffers for northern and Southern blots and has many other uses around the lab.
Catalog: AM9820
Size: 250 ml

GeneMapper® Software v4.1 30 Day Demo

Category: Products & Services > Instruments, Software, & Accessories > Software > Sequencing & Genetic Analysis Software

The GeneMapper® Software v4.1 Demo will install client and server configurations on the same computer. The demo version has full functionality for 30 days from installation and can be upgraded to the full version upon purchase of GeneMapper® Software v4.1 Full. GeneMapper® Software is a flexible genotyping software package that provides DNA sizing and quality allele calls for all Life Technologies electrophoresis-based genotyping systems. This software specializes in multiapplication functionality, including amplified fragment length polymorphism (AFLP®), loss of heterozygosity (LOH), microsat...
Catalog: 4366851
Size: 1 copy

Ion Xpress™ Barcode Adapters 1-16 Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Catalog: 4471250
Size: 1 kit

Vivid Colors™ pcDNA™6.2/C-EmGFP-GW/TOPO® Mammalian Expression Vector

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO® Expression Vectors (Figure 1) allow you to rapidly clone your gene and fuse it to the widely used and well characterized Fluorescent Proteins (FPs) from the jellyfish Aequorea victoria (1, 2). These powerful TOPO® cloning vectors contain the Emerald Green Fluorescent Protein (EmGFP) or the Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein (Figure 2). Both FPs have been humanized for optimal mammalian expression (3). The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO® Expression Vectors offer: • Top...
Catalog: K359-20
Size: 20 reactions

pcDNA™3.1/V5-His TOPO® TA Expression Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq -amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants. In addition, the vector includes the following features: Strong CMV promoter for high-level, constitutive expression. C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody. C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating...
Catalog: K4800-40
Size: 40 reactions

SuperScript® II Reverse Transcriptase

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Reverse Transcriptase

SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics. • Yield – Reduced RNase H activity results in greater first-strand cDNA yields • Size of ...
Catalog: 18064-071
Size: 4 × 10,000 units

DynaMag™-2 Magnet

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers

The new DynaMag™-2 magnet is optimized for efficient magnetic separation in small sample volumes using Dynabeads®. The DynaMag™-2 magnet combines a strong magnetic attraction for efficient separations with a flexible and smart ergonomic design. The top rack can be easily removed from the magnet in the base, ready for resuspension, vortexing, rotation or manual sample shaking. A center pin in the rack ensures equal vortexing of all tubes. The rack can also be removed and used to store tubes. The magnet can be used with all types of Dynabeads® for efficient isolation of proteins and nucleic acid...
Catalog: 12321D
Size: each

pcDNA™3.1/nV5-DEST

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12290-010
Size: 6 µg

Covaris S2 /S220 System Tubes & Caps

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories

Borosilicate round bottom tubes (13 mm x 65 mm) and caps for use with the Covaris™ S2 System. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4399054
Size: 125 tube

POP-6™ Polymer for the 310 Genetic Analyzer

Category: Products & Services > Instruments, Software, & Accessories > Capillaries & Polymers > Polymers

POP-6™ is a denaturing polymer used on the 310 Genetic Analyzer. POP-6™ Polymer is used for sequencing applications. • Easy to Use: Pre-formulated, which not only saves time but also saves reproducibility by ensuring consistent formulation. • Robust Formulation: Formulated for sequencing and fragment analysis applications. • Replenishable: Allow capillaries to be used multiple times. Reproducible Results POP™ polymers dynamically coat the capillary wall to control electro-osmotic flow. Their definite quality and uniform consistency eliminate guesswork and help to ensure reproducibility. POP Po...
Catalog: 402837
Size: 3 ml

Streptavidin, Alexa Fluor® 647 conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin

Alexa Fluor® 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western ...
Catalog: S-21374
Size: 1 mg

Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize cross-reactivity, it has been adsorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum. We also provide a version of the same antibody that has been cross-adsorbed against additional species (A-11034).
Catalog: A-11008
Size: 0.5 ml

N-2 Supplement (100X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > B-27, G-5, and N-2 Supplements

N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s N-1 formulation. N-2 Supplement is intended for use with Neurobasal® media. N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate. N-2 Supplement may be used with Neurobasal® media supplemented with growth factors such as bFGF and E...
Catalog: 17502-048
Size: 5 ml

Lipofectamine® LTX & Plus Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

Lipofectamine® LTX Reagent offers a new, advanced solution for gene expression studies in hard-to-transfect cells. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, difficult-to-transfect, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in experiments or cell lines. A certificate of origin may be obtained by contacting Technical Support. ...
Catalog: A12621
Size: 0.1 ml

Partek® Genomics Suite™ Software (Government)

Category: Products & Services > Instruments, Software, & Accessories > Software > Sequencing & Genetic Analysis Software

Partek® leverages a 17 year commercial history—founded on sound statistical analysis and expanded from a decade of microarray analysis--to today's semiconductor-based sequencing. Partek® Genomics Suite™ software is a full-featured software package for the analysis, comparison, and integration of all your genomic data. This product, which can be ordered through the Life Technologies website, is supplied directly by Partek Incorporated. Partek® Genomics Suite™ Software Gives You: • Sophisticated capabilities for DNA-seq, ChIP-seq, and RNA-seq experiments • Statistical analysis of each base pair ...
Catalog: 4462922G
Size: 1 each

pYES2

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Yeast Expression Vectors

The pYES2 vector is designed for native expression of your protein of interest in S. cerevisia e. It contains the URA3 gene for selection in yeast and 2µ origin for high-copy maintenance.
Catalog: V825-20
Size: 20 µg

3500/3500xL Sequencing Standards, BigDye® Terminator v3.1

Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards

The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: 4404312
Size: 4 tubes

Silencer ® GFP (eGFP) siRNA (inc. 2 nmol Neg. Control siRNA)

Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls

Ambion® Silencer ® GFP (eGFP) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using oth...
Catalog: AM4626
Size: 5 nmol

F(ab')2 Fragment of Goat Anti-Rat IgG (H+L), Horseradish Peroxidase Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Affinity purified F(ab')2 gragment of goat anti-rat IgG conjugated to horseradish peroxidase. To minimize cross-reactivity, this antibody has been absorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Catalog: A10548
Size: 0.5 mg

Normal Rabbit IgG

Category: Products & Services > Antibodies & Secondary Detection Reagents > Isotype Antibody Controls

Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Catalog: 10500C
Size: 5 ml

UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals

UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl. When mixtures are extracted with Phenol:Chloroform:Isoamyl Alcohol, proteins are denatured and collected in the organic phase or at the interphase, while nucleic acids remain in the aqueous phase. UltraPure™ Phenol:Chloroform:lsoamyl Alcohol contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated amber bottles. Performance and Quality Te...
Catalog: 15593-031
Size: 100 ml