The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis. • Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit • Simple magnetic bead workflow—no spin columns • Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors Maximize Total ...
Size: 96 preps
The pcDNA™6⁄TR vector is part of the T-REx™ System (catalog K102001), which yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2). The regulatory vector, pcDNA™6⁄TR, is provided for high-level expression of the tetracycline repressor (TR) protein. This vector expresses the Blasticidin resistance gene for rapid selection of ...
Size: 20 µg
The thiol-reactive Alexa Fluor 546 C 5 maleimide and its conjugates are perfect for applications that require orange-fluorescent probes. Spectrally similar to the Cy3 dye, the Alexa Fluor 546 dye is readily excited by the 546 nm line of the mercury-arc lamp and the 543 nm line of the He-Ne laser.
Size: 1 mg
Acid Phenol:Chloroform:IAA (125:24:1) is premixed and supplied at pH 4.5 ± 0.2. Provided in one bottle of 100 mL. In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, an...
Size: 100 ml
Ion 318™ Chip Kits contain chips for sequencing runs using the Personal Genome Machine™ System, the first PostLight™ semiconductor-based sequencer. The Ion 318™ Chip detects polymerase-driven base incorporation and translates this information into digital form. By eliminating the use of light-producing chemistry and optical systems, this revolutionary advance in next-generation sequencing technology makes massively parallel sequencing affordable to nearly every lab. Ion 318™ Chips provide: • Easy handling and loading of templated Ion Sphere™ particles • Over 11 million wells per chip • Compati...
Size: 8 pack
pcDNA™4/HisMax is specifically designed to maximize protein expression in a variety of mammalian cells. The vector contains the QBI SP163 translational enhancer to increase expression levels two- to five-fold above those seen with the promoter alone (Figure 1). In addition to SP163-enhanced expression, pcDNA™4/HisMax includes a cleavable N-terminal Xpress™ tag for rapid detection of recombinant protein with an Anti-Xpress™ Antibody (Figure 2). pcDNA™4/HisMax is available TOPO® Cloning-ready for five-minute cloning of Taq amplified PCR products.
Size: 20 µg
AccuPrime™ Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accesory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1). AccuPrime™ Pfx SuperMix is ideal for high-fidelity, high-specificity amplification of DNA fragments for applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. Thermostable AccuPrime™ proteins enhance primer-templa...
Size: 200 reactions
Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 591/608 nm Superior to antibody staining Optimal for fixed and permeabilized samples Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually i...
Size: 300 units
This sequencing buffer is optimized for use with BigDye® Terminator v1.1 and v3.1 Cycle Sequencing Kits. For Research Use Only. Not for use in diagnostics procedures.
Size: 233 ml
The Alexa Fluor 488 hydrazide is a low molecular weight, cell membrane—impermeant, aldehyde-fixable molecule that can be used as a cell tracer. This green-fluorescent polar dye can be loaded into cells by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Size: 1 mg
The Ambion® Buffer Kit provides high quality, RNase free buffers for critical RNA analysis procedures. The kit consists of one 100 mL bottle of each of the following solutions: 1 M Tris pH 7.0, 1 M Tris pH 8.0, 5 M NaCl, 1 M MgCl 2 , 0.5 M EDTA pH 8.0, 2 M KCl, 5 M Ammonium Acetate, and DEPC-treated H 2 O. Ammonium Acetate is sterile filtered. All other solutions are autoclaved post-packaging to ensure sterility. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
Size: 1 set
Size: 12 × 1 L
The new Gibco® bottle has its own bottle weight for keeping order in the water bath! The bottle weight fits the new 500 mL and 1 L Gibco® bottles, keeping them upright in the water to further reduce your risk of contamination. Product Use For Research Use Only. Not for use in diagnostic procedures.
Size: 1 each
Gibco® MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Vitamin Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001...
Size: 100 ml
The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Size: 25000 reactions
Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway® Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (catalog K5330-00). The pLenti6.3⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Poly...
Size: 1 kit
Magnetic Stand is part of the I-SAGE kit
Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L) is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. To minimize cross-reactivity, it has been adsorbed against human IgG and human serum prior to conjugation. We also provide a version of the same antibody that has been cross-adsorbed against additional species (A-11029).
Size: 0.5 ml
Immunogen: Rat IgG (H+L) Specificity: Rat IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Size: 1 ml
SNAP spontaneously releases nitric oxide (NO) under physiological conditions.
Size: 25 mg
The Applied Biosystems® MicroAmp® Optical 96-Well Reaction Plate is optimized to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a ninety-six well format is compatible with Applied Biosystems® 96-Well Real-Time PCR Systems and Thermal Cyclers. • Frosted to minimize interfering fluorescence from wells of cycling block in real-time PCR applications • Screened to eliminate auto-fluorescent plates • Unique design provides a barrier to ambient air to help insure well-to-well temperatur...
Size: 10 plates
Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR product is directionally cloned with efficiencies typically >99%. The pDONR™/Zeo vector haa a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
Size: 6 µg
The Vybrant® Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types. In this assay, cells are labeled with calcein AM and allowed to adhere. After removal of nonadherent cells, calcein fluorescence is used to calculate the number of adherent cells.
Size: 1 kit
The Nick Translation System is ideal for both radioactive and non-radioactive labeling of DNA. The Nick Translation System: Provides five pre-mixed nucleotide solutions for flexibility in labeled nucleotide incorporation Yields >10 8 cpm/µg control DNA using [ - 32 P]-dCTP Labels 1 µg of DNA in one reaction
Size: 50 reactions
The mir Vana™ qRT-PCR Detection Kit has been optimized to amplify specific small RNA using qRT-PCR primer sets (not included), enabling accurate quantitation of miRNA expression levels. The novel detection system can be used to characterize miRNA expression via traditional endpoint RT-PCR or real-time RT-PCR using SYBR® Green I. • Quantitative results in less than 3 hours • Detection from as little as 25 pg of total RNA • Suitable for either real-time or end-point RT-PCR • Ideal for validating expression profiling data and high-throughput applications. MicroRNAs (miRNAs) are evolutionarily con...
Size: 200 reactions
The NuPAGE® MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels. NuPAGE® MOPS SDS Running Buffer is recommended for separating medium- to large-sized proteins. Use the Right Buffer to Optimize Protein Separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with the NuPAGE® Bis-Tris gels. Using MOPS buffer allows proteins to run slower vs. MES buffer. Get Consistent, Convenient High-Quality Results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buf...
Size: 5 L
The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Size: 250 µl
Ambion® Lambda DNA is digested to completion with Hind III. This generates fragments of the sizes 23130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp. These DNA molecular mass markers are supplied with a protocol describing easy labeling of the markers with radioisotopes. They can also be stained with ethidium bromide. One tube containing 200 μg is provided.
Size: 200 µg
The SeeBlue® Plus2 Pre-Stained Standard has been optimized for compatibility with NuPAGE® and Bolt™ gels, which use Life Technologies' Bis-Tris gel formulation. The SeeBlue® Plus2 Pre-Stained Standard contains 10 proteins: eight blue-dyed proteins and two proteins with contrasting colors, allowing easier band identification. The SeeBlue® Plus2 Standard allows you to easily monitor molecular weight ranges during electrophoresis and quickly evaluate Western transfer efficiency. The SeeBlue® Plus2 Standard offers: • 10 polypeptides resolved into 8 blue and 2 colored bands in the range of 4–250 kD...
Size: 500 µl
EDAC is a water-soluble carbodiimide that can be used to crosslink biological substances that contain carboxylate acids and primary amines. Protocols for crosslinking molecules with this reagent can be found in Bioconjugate Techniques , by G.T. Hermanson (B-7884).
Size: 100 mg
The pcDNA™3.3-TOPO® vector is designed to deliver exceptionally high levels of transgene expression in adherent cells as well as in the FreeStyle™ MAX CHO and FreeStyle™ MAX 293 suspension cell systems. The pcDNA3.3™-TOPO® TA Cloning® Kit is supplied with the TOPO®-adapted plasmid vector, all reagents for cloning, and One Shot® TOP10 competent cells. The pcDNA™3.3-TOPO® vector offers the following benefits: • TOPO® adapted for cloning PCR products with >85% efficiency • High-level gene expression in a wide range of mammalian cells due to the native cytomegalovirus (CMV) immediate-early promote...
Size: 20 reactions
The DynaMag™-5 Magnet is intended for use with Dynabeads® for the immunomagnetic separation of cells. The DynaMag™-5 magnet accommodates tubes from 11–16 mm in diameter (e.g., flow cytometry tubes and most blood collection tubes), with an optimal working volume of 1–5 mL. The magnet holds 8 tubes and is compatible with all Dynabeads® (1–4.5 µM in diameter). When performing cell separation using Dynabeads®, a DynaMag™ magnet is required. Different magnets are optimized for different working volumes and tube sizes. See our complete overview of the DynaMag™ magnets . The DynaMag™-5 magnet contain...
Size: 8 samples
Ambion® RNaseZap® is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® onto the surface to be decontaminated and rinse it off with RNase-free water. Supplied in a single 4 liter cubitainer. • Completely removes RNase contamination from glass and plastic surfaces • Excels at removing high levels of RNase contamination whereas similar products fail • Proven effective at removing high concentrations of dried-on RNase A • Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free Working with RNA requires that special measures be...
Size: 4 L
Need Results Fast Applied Biosystems® TaqMan® Fast Universal Master Mix (2x), No AmpErase® UNG delivers results in 40 minutes for 40 cycles of PCR in a 20 µL reaction volume using the Applied Biosystems® 7900HT and Fast 7500 Fast Real-Time PCR Systems. • Everything you need for TaqMan®-based qPCR amplification and detection in a convenient single-tube format. TaqMan® Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR • TaqMan® Fast Universal PCR Master Mix (2x), No AmpErase® UNG is compatible with TaqMan® Gene Expressio...
Size: 500 reactions
This purified mouse anti-NY-ESO-1 monoclonal antibody recognizes human NY-ESO-1. NY-ESO-1 is a member of the cancer/testis (CT) family of antigens, which are expressed in a range of malignant neoplasmas such as melanoma and carcinomas of the breast, ovary and lung, but not in normal tissue except in testis. NY-ESO-1 is a classic CT antigen discovered during a SEREX analysis of esophageal cancer. It is a 22–23 kDa hydrophobic protein. Validated applications for anti-NY-ESO-1 are western blotting and immunohistochemistry (paraffin). Antibody Specifications: • Applications: Validated applications...
Size: 100 µg
Ambion® Silencer ® Select siRNAs are the result of recent improvements in siRNA design combined with novel chemical modifications yielding unrivaled knockdown efficiency and specificity. Our best performing siRNA for cell culture work. Silencer® siRNAs are unmodified 21 nucleotide siRNAs that have been designed with an older, yet rigorously tested design algorithm. A lower cost alternative to Silencer® Select siRNAs, but without some of the performance enhancements.
Size: 5 nmol
The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of the High Capacity RNA-to-cDNA™ Kit: • Fast —Short reaction time (typically 30–60 min) • Convenient —Easy workflow with few pipetting steps (2 tubes) • Reliable —Reliable reverse transcription of both abundant and limited targets • Flexible —Optimized 2-step protocol enabling multiple PCR reactions from a single reverse transcription reaction Streamline Your cDNA Synthesis ...
Size: 50 reactions
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1). PCR SuperMix contains recombinant Taq DNA polymerase for routine PCR of fragments up to 5kb.
Size: 100 reactions
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Size: 10 ml
pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid, pcDNA™5⁄FRT⁄TO⁄CAT, is also included for use as a positive control for transfection and expression in your Flp-In™ T-REx™ host cell line of choice. The pcDNA™5⁄FRT⁄TO vector contains the following elements: • A hybrid human cytomegalovirus (CMV)⁄TetO2 promoter for high-level, tetracycline-regulated expression of the gene of interest in a wide range of mammalian cells • Multiple cloning site with 10 unique restriction sites to facilitate cloning the gene of interest ...
Size: 20 µg
The Ambion® PARIS™ system is for the isolation of both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The kit contains sufficient reagents for 50 purifications from1 to 75 mg of tissue or 100 to 10 7 cells each. • Fast, simple procedure • Isolate nuclear and cytoplasmic RNA and protein from cultured cells • Isolate total RNA and protein from cultured cells or tissues • No phenol extraction or alcohol precipitation • Reduces time, cost, and variability between independent exper...
Size: 50 purifications
The Ambion® RecoverAll™ Total Nucleic Acid Isolation Kit is for the extraction of total nucleic acid from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up to four 20 µm sections, or up to 35 mg of unsectioned core samples each. Features of the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE: • Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue • No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon • Typical yields are...
Size: 40 purifications
MitoTracker Red CMXRos is a red-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.
Size: 20 × 50 µg
MAX Efficiency® Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to rec A1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences (1) and tandem array genes (2). MAX Efficiency® Stbl2™ Competent Cells offer: >1 x 10 9 transformants/flg for cloning rare sequences Elimination of mcr BC- hsd RMS- mrr and mutation of mcr A allows cloning of methylated genomic sequences (1) Unique genotype stabilizes direct repeat and retroviral sequences such as SIV and HIV (1) Greatly ...
Size: 1 ml
Applied Biosystems® AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus ( Taq ) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy Its thermal activity profile makes it good ...
Size: 250 units
Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Size: 10 ml
Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features: • Ability to support suspension and stationary hybridomas and myelomas • Formulated without L-glutamine for stability • Animal origin-free, protein-free and chemically defined formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medi...
Size: 1,000 ml
Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial. • Premixed components stored at 2–8°C significantly reduce assay setup time • SYBR® Green I dye detects double-stranded DNA, so specific probes are not required • AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance • dUTP significant...
Size: 200 reactions
The SuperScript® III First-Strand Synthesis System for RT-PCR provides all the necessary components for synthesis of high-quality first-strand cDNA from total or poly(A)+ RNA. Used with PCR, this functionally tested system enables you to detect rare messages and generate enough material for cloning. The SuperScript® III First-Strand Synthesis System: Uses SuperScript® III RT for higher cDNA yields (Figure 1) Includes all components needed for first-strand cDNA synthesis Consists of optimized reagents for more robust performance Contains a streamlined protocol Detects template molecules from as...
Size: 50 reactions
Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a powder and should be m...
Size: 5 g