Products from Midi-Gel Adapters to Ion 318™ Chip Kit (8 Pack)

Midi-Gel Adapters

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Other Gel Electrophoresis Accessories

The Midi Gel Adapter is designed for use with the Novex® Midi Gel Cassette (NuPAGE® or Tris-Glycine Gels) to perform electrophoresis using a BioRad Criterion™ Cell. The Midi Gel Adapter is also supplied with the Novex® Midi Gels with Adapters Kit.
Catalog: WA0999
Size: 10⁄box

Soybean Trypsin Inhibitor, powder

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin Inhibitors

Soybean Trypsin Inhibitor is one of several protease inhibitors found in soybeans. Trypsin is inhibited at a molar ratio of 1:1, while chymotrypsin and plasmin are inhibited to a lesser extent. Soybean trypsin inhibitor will also inhibit other serine proteases. Gibco® Soybean Trypsin Inhibitor, powder has an activity greater than 7,000 BAEE units per mg. A 0.5 mg/mL Soybean Trypsin Inhibitor stock solution should be made by dissolving the non-sterile powder in 0.01M Borate Buffer or Dulbecco's Phosphate Buffered Saline (DPBS), without calcium and magnesium. Filter sterilize the stock solution ...
Catalog: 17075-029
Size: 1 g

NuPAGE® MES SDS Running Buffer (for Bis-Tris Gels only) (20X) (500 ml)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers

NuPAGE® MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels. NuPAGE® MES SDS Running Buffer is recommended for separating small- to medium-sized proteins. Use the Right Buffer to Optimize Protein Separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with the NuPAGE® Bis-Tris gels. Using MES buffer allows proteins to run faster vs. MOPS buffer. Get Consistent, Convenient High-Quality Results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers a...
Catalog: NP0002
Size: 500 ml

Applied Biosystems® ViiA™ 7 Real-Time PCR System with Fast 96-Well Block

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Real-Time PCR Instruments

The ViiA™ 7 Real-Time PCR System combines all of the qPCR features you want in a single high performance instrument, so that you can optimize your research productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, the ViiA™ 7 System offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or fast-cycling 384- or 96-well plates and TaqMan® Array Micro Fluidic Cards and Reagents makes high-productivity real-time PCR accessible to any size l...
Catalog: 4453535
Size: 1 instrument

High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 1000 x 20 µL reactions. • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (for...
Catalog: 4374967
Size: 1000 reactions

Human ACTB (Beta Actin) Endogenous Control (VIC® / MGB Probe, Primer Limited)

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Applied Biosystems® Human ACTB (beta actin) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_0...
Catalog: 4326315E
Size: 1000 reactions

StepOne™ Real-Time PCR System Spectral Calibration Kit

Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Real-Time PCR Instrument Calibration Kits & Reagents

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and ROX™ dyes on the StepOne™ Real-Time PCR System. Features of the StepOne™ Real-Time PCR System Spectral Calibration Kit include: • 48-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, and ROX™) are provided for quick and easy calibration of the StepOne™ Real-Time PCR System • Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture • Includes the Passive Reference I dye standard for sig...
Catalog: 4371433
Size: 1 kit

AB-Human Serum

Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards

AB-Human Serum (heat-inactivated)
Catalog: 34005-100
Size: 100 ml

TaqMan® OpenArray® Genotyping Plate, Custom Format 192 QuantStudio™ 12K Flex

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

TaqMan® OpenArray® Genotyping Plate, Custom Format 192 QuantStudio™ 12K Flex
Catalog: 4471117
Size: 10 pack

Magnetic Stand-96

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers

For the paramagnetic bead precipitation from standard 96-well U-bottom microplates and 0.2 mL PCR plates with no additional accessories. The Ambion® stand has a clear polyacryl base in a standard SBS 96-well microplate footprint. The use of 24 extremely strong permanent magnets guarantees easy and fast (as little as 30 seconds) magnetic separation. It sits flat on a lab bench, as well as in a microplate adapter on robotic workstations. The Magnetic Stand-96 has a total of 24 magnets (4 wells surround each magnet), so the magnetic beads are attracted to one side of each well. Accessory Product ...
Catalog: AM10027
Size: 1 each

One Shot® PIR1 Chemically Competent E. coli

Category: Products & Services > Competent Cells & Strains > Other Competent Cells & Strains

The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA str...
Catalog: C1010-10
Size: 10 reactions

Acrylamide/Bis 19:1 40% (w/v) Solution

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Acrylamide & Polymerizing Agents

Ambion® Acrylamide/Bis 19:1 is a 40% (w/v) solution of acrylamide (38%) and bis-acrylamide (2%) ideal for use in ribonuclease protection assay, sequencing gels, and sizing DNA or RNA fragments. Supplied in one bottle containing 500 mL. The solution is provided in a ready-to-use form, reducing the dust, inhalation, and contact hazard associated with weighing and preparing acrylamide and bis-acrylamide powders and solutions. Concentrations are carefully controlled and all solutions are micro-filtered prior to packaging. All Ambion® acrylamides are rigorously tested for contaminating non-specific...
Catalog: AM9022
Size: 500 ml

Buffer Dam for XCell SureLock® and XCell II™

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Other Gel Electrophoresis Accessories

Replacement Parts for Electrophoresis Apparatus
Catalog: EI0012
Size: each

TaqMan® Universal PCR Master Mix, No AmpErase® UNG, 2-Pack (2 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4364341
Size: 400 reactions

pSecTag2 A, B, & C

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features: Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2) Cytomegalovirus (CMV) promoter for high-level constitutive expression C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chel...
Catalog: V900-20
Size: 20 µg

pcDNA™4/HisMax A, B, & C

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

pcDNA™4/HisMax is specifically designed to maximize protein expression in a variety of mammalian cells. The vector contains the QBI SP163 translational enhancer to increase expression levels two- to five-fold above those seen with the promoter alone (Figure 1). In addition to SP163-enhanced expression, pcDNA™4/HisMax includes a cleavable N-terminal Xpress™ tag for rapid detection of recombinant protein with an Anti-Xpress™ Antibody (Figure 2). pcDNA™4/HisMax is available TOPO® Cloning-ready for five-minute cloning of Taq amplified PCR products.
Catalog: V864-20
Size: 20 µg

293FT Cell Line

Category: Products & Services > Eukaryotic Cells > Cell Lines > Mammalian Cell Lines

The 293FT Cell line is ideal for generating high-titer lentivirus using the ViraPower™ Lentiviral Expression System. The 293FT Cell Line is a fast-growing, highly transfectable clonal isolate derived from human embryonal kidney cells transformed with the SV40 large T antigen. When a ViraPower™ expression vector and the ViraPower™ Lentiviral Packaging Mix are co-transfected into 293FT cells, high levels of the viral RNA and the gag⁄pol and rev proteins required for packaging are produced. Advantages • Fast growing • Highly transfectable • Produces high viral titers • Allows very high levels of ...
Catalog: R700-07
Size: 3 × 10^6 cells

One Shot® BL21 Star™ (DE3) Chemically Competent E. coli

Category: Products & Services > Competent Cells & Strains > Expression Competent Cells

One Shot® BL21 Star™ (DE3) E. coli are chemically competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., Champion™ pET vectors) . One Shot® BL21 Star™ (DE3) Chemically Competent cells are controlled for a transformation efficiency of 1 x 10 8 cfu/ µg plasmid DNA. • A genotype that promotes high mRNA stability and protein yield • Ideal for use with low copy number, T7 promoter-based plasmids • The single tube, single use format allows all transformation steps to take place i...
Catalog: C6010-03
Size: 20 reactions

MicroAmp® Optical Adhesive Film

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Adhesive Film & Other Plate-Related Accessories

The Applied Biosystems® MicroAmp® Optical Adhesive Film reduces the chance of well-to-well contamination and sample evaporation when applied to a microplate. • Pressure-sensitive adhesive provides a tight seal over every well • Will not interfere with sample reading • Press-N-Seal: Align properly to the plate, then press to seal • Easy to apply-will not stick to your glove Prevent Loss of Precious Samples Use this optically-clear adhesive film to seal the samples into the wells of a microplate. This will reduce the possibility of cross-contamination between sample wells and help ensure consist...
Catalog: 4360954
Size: 25 covers

William's E Medium, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Williams' Media E

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. This media can also be used for growing hepatocyte cells of different species, such as human HepaRG™ cells. This William's E Medium is modified as follows: With Without • Phenol Red • L-glutamine • HEPES
Catalog: 12551-032
Size: 500 ml

Medium 199, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Media 199

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. Life Technologies offers a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This M199 is modified as follows: With Without • L-glutamine • Phenol Red • HEPES
Catalog: 11043-023
Size: 500 ml

Pathatrix® Magnetic Capture Rack

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers

The Pathatrix® Magnetic Capture Rack makes it easy to link the Pathatrix® Auto workflow to specific endpoint detection technologies including Idaho R.A.P.I.D. ® LT, bioMerieux VIDAS®, and BioControl GDS instruments. The magnetic rack is used to remove the paramagnetic particles after lysis and prior to detection.
Catalog: MAGNETICRACK
Size: 1 each

CapSure® HS LCM Caps (Alignment Tray & Incubation Block Required, not Included)

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Counting & Flow Cytometer Accessories

Specially designed for use with the ARCTURUS® Laser Capture Microdissection (LCM) instruments, CapSure® HS and CapSure® Macro LCM Caps enable the precise and rapid extraction of pure populations of cells from heterogeneous sample preparations. CapSure Caps may be used to isolate individual cells or thousands of cells on a single cap. Key product features: • Gentle application – non-damaging microdissection preserves the integrity of the captured material • Versatile extraction – capture both large and small amounts of cells Ensure the Integrity of Microdissected Cells for Downstream Molecular ...
Catalog: LCM0214
Size: 32 caps

RPMI 1640 Medium, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > RPMI Medium 1640

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This RPMI is modified as follows: With Without • L-glutamine • Phenol Red • HEPES
Catalog: 11835-055
Size: 10 × 500 ml

CD44 Recombinant Human Protein, His Tag

Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers

Cluster of Differentiation 44 (CD44), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Pro 220) of human CD44 (P16070-1) fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0...
Catalog: 12211-H08H-5
Size: 5 µg

2-( N -(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG)

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Metabolic Assay Kits

2-NBDG is a fluorescent glucose analog that has been used to monitor glucose uptake in live cells, as an indicator of cell viability. Although sensitive to its environment NBD fluorescence typically displays excitation/emission maxima of ~465/540 nm and can be visualized using optical filters designed for fluorescein.
Catalog: N13195
Size: 5 mg

SeeBlue® Plus2 Pre-Stained Standard

Category: Products & Services > Controls, Standards & Ladders > Protein Standards & Ladders > Prestained Protein Standards

The SeeBlue® Plus2 Pre-Stained Standard has been optimized for compatibility with NuPAGE® and Bolt™ gels, which use Life Technologies' Bis-Tris gel formulation. The SeeBlue® Plus2 Pre-Stained Standard contains 10 proteins: eight blue-dyed proteins and two proteins with contrasting colors, allowing easier band identification. The SeeBlue® Plus2 Standard allows you to easily monitor molecular weight ranges during electrophoresis and quickly evaluate Western transfer efficiency. The SeeBlue® Plus2 Standard offers: • 10 polypeptides resolved into 8 blue and 2 colored bands in the range of 4–250 kD...
Catalog: LC5925
Size: 500 µl

Goat Anti-Rabbit IgG - HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Immunogen: Rabbit IgG Specificity: Rabbit IgG Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Catalog: 65-6120
Size: 1 ml

MicroAmp® Fast Optical 96-Well Reaction Plate with Barcode, 0.1 mL

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > PCR Plates

The Applied Biosystems® MicroAmp® Fast 96-Well Reaction Plate with Barcode is an integral component of the Applied Biosystems® Fast PCR System, which reduces PCR reaction time from 2 hours to as little as 25 minutes. • Maximum thermal conductivity for precise thermal cycling. • Reproducible, specific and sensitive PCR results in just 25 minutes. • Validated with other system components for consistent results. • Each reaction plate includes a unique serialized, eight character number label (bar code) that is user-readable and machine-readable to prevent tracking errors. Increased Thermal Contac...
Catalog: 4346906
Size: 20 plates

Phenol:Chloroform:IAA, 25:24:1, pH 6.6 (raise to pH 7.9 with Included Buffer)

Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals

Ambion® Acid Phenol:Chloroform:IAA (25:24:1) is premixed and supplied at pH 6.6 (pH 7.9 ± 0.2), with included buffer. Acid Phenol:Chloroform:IAA is provided in one bottle of 100 mL. In RNA and DNA extraction procedures, Acid Phenol:Chloroform:IAA (pH 7.9) helps to stabilize the interface and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more conv...
Catalog: AM9730
Size: 100 ml

10X BlueJuice™ Gel Loading Buffer

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

BlueJuice™ Gel Loading Buffer is designed for easy loading and tracking of DNA samples in agarose or native polyacrylamide gels.
Catalog: 10816-015
Size: 3 × 1 ml

Applied Biosystems® ViiA™ 7 Real-Time PCR System with 384-Well Block

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Real-Time PCR Instruments

The ViiA™ 7 Real-Time PCR System combines all of the qPCR features you want in a single high performance instrument, so that you can optimize your research productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, the ViiA™ 7 System offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or fast-cycling 384- or 96-well plates and TaqMan® Array Micro Fluidic Cards and Reagents makes high-productivity real-time PCR accessible to any size l...
Catalog: 4453536
Size: 1 instrument

Ion Sphere™ Quality Control Kit

Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Sequencing Workflow Controls

The Ion Sphere™ Quality Control Kit furnishes a complete set of reagents for performing templated bead quality control using the Qubit® 2.0 Fluorometer. This comprehensive kit contains calibration controls, buffers, and a probe mix for quality assessment of pre-enriched and post-enriched template-positive Ion Spheres. The powerful combination of the Ion Sphere™ Quality Control Kit and the Qubit® 2.0 Fluorometer gives you greater experimental control and significant time and cost savings. By providing the confidence to generate high quality sequencing data with every run, this quality control p...
Catalog: 4468656
Size: 1 kit

TaqMan® Copy Number Reference Assay, RNase P, Human

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (NCBI Build 37/hg19) and are required for relative quantitation of copy number targets. TaqMan® Copy Number Assays quantitate the gene of interest and normalize t...
Catalog: 4403326
Size: 750 reactions

RPMI 1640 Medium, no Glucose

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > RPMI Medium 1640

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This RPMI is modified as follows: With Without • L-glutamine • Glucose • Phenol Red • HEPES
Catalog: 11879-020
Size: 500 ml

MicroAmp® Cap Installing Tool (Handle)

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > PCR Machine (Thermal Cycler) Accessories

The MicroAmp® Cap Installing Tool helps you install strips of MicroAmp® caps onto MicroAmp® reaction tubes in a tray⁄retainer set or 96-well reaction plate. • Available in handle style • Seal 8 or 12 cap strips • Recessed cup for pressing caps onto tubes • Convenient tool ensures proper sealing Press and Seal Caps The MicroAmp® Cap Installing Tool enables you to ergonomically seal MicroAmp® reaction tubes and plates. This tight seal will prevent well-to-well contamination and sample loss. Used With MicroAmp® Optical 8-Cap Strip, MicroAmp® Optical 8-Tube Strip, 0.2 ml, MicroAmp® Optical 96-Well...
Catalog: 4330015
Size: 1 tool

Zeocin™ Selection Reagent, (Liquid)

Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics

Gibco® Zeocin™ selective reagent is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus . Gibco® Zeocin™ selective reagent acts by binding to and cleaving DNA, leading to cell death. Gibco® Zeocin™ selective reagent is used for selection of yeast, bacterial and mammalian cell cultures. This product is supplied at 100 mg/ml in sterile water and is used at a working concentration ranging between 50 - 400 µg/ml depending on the cell type. Life Technologies offers a variety of antibiotics and antimycotics for cell culture applications. Product Use For Res...
Catalog: R250-05
Size: 50 ml

Invitrogen Magnetic Stand

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers

Magnetic Stand is part of the I-SAGE kit
Catalog: R670-01
Size: each

Streptavidin - Cy5

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin

Reactivity: CY5-SA reacts with biotin.
Catalog: 43-4316
Size: 1 mg

8% TBE Gels 1.0 mm, 10 Well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Polyacrylamide Gels

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Catalog: EC6215BOX
Size: 1 box

TaqMan® RNase P Control Reagents Kit

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The RNase P gene is a single-copy gene that encodes the RNA moiety for the RNase P enzyme. Applied Biosystems® TaqMan® RNase P Control Reagents were designed with limiting primer concentrations to be used as the endogenous reference in multiplex reactions. Multiplex PCR is the use of more than one probe and primer pair in the same tube. This kit is designed to perform a 5' nuclease assay with TaqMan® Universal PCR Master Mix using the provided human genomic DNA as template. • Pre-designed primers and TaqMan® probe eliminate assay design • Easy-to-use primer and TaqMan® probe mix minimizes assa...
Catalog: 4316844
Size: 1000 reactions

Gateway® pDEST™17 Vector

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Bacterial Expression Vectors

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11803-012
Size: 6 µg

pENTR™ 3C Dual Selection

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Gateway® Entry Vectors - pENTR

Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in...
Catalog: A10464
Size: 10 µg

ACK Lysing Buffer

Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents

Gibco® ACK (Ammonium-Chloride-Potassium) Lysing Buffer is suitable for use in the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library. cGMP Manufacturing and Quality System Gibco® ACK Lysing Buffer is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
Catalog: A10492-01
Size: 100 ml

Rat GAPD (GAPDH) Endogenous Control (VIC®/MGB Probe, Primer Limited)

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Applied Biosystems® Rat GAPD (GAPDH) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: GAPDH RefSeq: NM_017008.3 Probe Exon Location: 3 Ampl...
Catalog: 4352338E
Size: 1000 reactions

Alexa Fluor® 488 hydrazide, Sodium Salt, 1 mg

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Aldehyde-, Ketone-, & Carboxylate-Reactive Labels

The Alexa Fluor 488 hydrazide is a low molecular weight, cell membrane—impermeant, aldehyde-fixable molecule that can be used as a cell tracer. This green-fluorescent polar dye can be loaded into cells by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: A-10436
Size: 1 mg

UltraPure™ DNA Typing Grade® 50X TAE Buffer

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Running Buffers

UltraPure™ DNA Typing Grade® 50X TAE Buffer is a sterile-filtered solution of 2 M Tris-acetate and 50 mM EDTA. TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. To prepare a 1X solution, mix one volume of UltraPure™ 50X TAE Buffer with 49 volumes of distilled water. A 1X TAE Buffer solution contains 40 mM Tris-acetate and 1 mM EDTA with a pH of 8.3 at 23“C. Performance and Quality Testing: No DNase activity detected.
Catalog: 24710-030
Size: 1 L

NucBlue® Live ReadyProbes™ Reagent

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue® Live ReadyProbes™ Reagent we have formulated this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Live ReadyProbes™ Reagent: Ready-to-use liquid formulation of high-purity Hoechst 33342 No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or cell cult...
Catalog: R37605
Size: 1 kit

BigDye® Terminator v1.1 & v3.1 5X Sequencing Buffer

Category: Products & Services > Buffers & Chemicals > Sequencing Buffers & Reagents

This sequencing buffer is optimized for use with BigDye® Terminator v1.1 and v3.1 Cycle Sequencing Kits. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4336701
Size: 233 ml

Ion 318™ Chip Kit (8 pack)

Category: Products & Services > Slide & Chip Arrays > Semiconductor Chips

Ion 318™ Chip Kits contain chips for sequencing runs using the Personal Genome Machine™ System, the first PostLight™ semiconductor-based sequencer. The Ion 318™ Chip detects polymerase-driven base incorporation and translates this information into digital form. By eliminating the use of light-producing chemistry and optical systems, this revolutionary advance in next-generation sequencing technology makes massively parallel sequencing affordable to nearly every lab. Ion 318™ Chips provide: • Easy handling and loading of templated Ion Sphere™ particles • Over 11 million wells per chip • Compati...
Catalog: 4469497
Size: 8 pack