Products from Power Sybr® Green Pcr Master Mix, 1-Pack (1 X 5 Ml) to Pentr™ 4 Dual Selection

Power SYBR® Green PCR Master Mix, 1-Pack (1 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR • The optimized formulation contains highly purified AmpliTaq Gold® D...
Catalog: 4367659
Size: 200 reactions

Custom Media: N2B27 CUSTOM MEDIA

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media

Custom Media: N2B27 CUSTOM MEDIA
Catalog: ME100137L1
Size: 1 each

Primer Express® Software v3.0.1, 5-User License

Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software

The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination. • Enables individual oligonucleotide design for real-time PCR applications. • Supports assays based o...
Catalog: 4363993
Size: 1 license

UltraPure™ 1M Tris-HCI, pH 7.5

Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers

UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and Quality Testing: No DNase, RNase, or protease activity detected
Catalog: 15567-027
Size: 1 L

MEM ALPHA

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)

MEM ALPHA
Catalog: 12714010S
Size: 500 ml

mMESSAGE mMACHINE® SP6 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA • Extra convenience; cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped RNA Transcription will yield lar...
Catalog: AM1340M
Size: 25 rxns w⁄ Manual

PCR8/GW/TOPO

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > PCR Cloning Vectors

PCR8/GW/TOPO
Catalog: K252020SP
Size: 20 reactions

Bioruptor® UCD-200 with accessories for 12 x 0.5 ml Tubes & BioBox-200

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories

The Bioruptor® UCD-200 is a benchtop sonication device used to uniformly process up to 12 x 0.5 ml samples simultaneously in sealed tubes without aerosol formation. Consisting of a high-power ultrasound generating element located below a water bath, the Bioruptor® sonicates at a 20 kHz frequency, similar to a probe sonicator. The Bioruptor® comes with standard 0.5 ml accessories and the BioBox-200 for soundproofing. The Bioruptor® UCD-200 Provides: • Parallel processing of up to 12 DNA samples • Robust and reproducible results • Automated sample prep Get Reproducible DNA Shearing Easily and Au...
Catalog: 4465622
Size: 1 each

Goat Anti-Mouse IgG (H+L) - BT

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Immunogen: Mouse IgG (H+L) Specificity: Mouse IgG (H+L) Applications: Useful as a secondary antibody in many types of immunoassays, including: WB, ELISA, and Immunohistochemistry. ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of bac...
Catalog: 62-6540
Size: 2 ml

Novex® Power Supply Adapters

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Power Supplies for Gel Systems

The Novex® Power Supply Adapters allow you to connect the covered power leads of electrophoresis units to certain power supplies (for example, some power supplies from Bio-Rad Laboratories, Thermo Fisher Scientific). The following electrophoresis units from Invitrogen have covered power leads to be in compliance with the latest IEC (International Electrotechnical Commission) and EN (European) standards, and reduce the risk of electrical shock due to exposure to bare metal. • ZOOM® IPGRunner™ Mini-Cell and ZOOM® IEF Fractionator • XCell4 SureLock™ Midi-Cell • XCell6™ MultiGel Unit • XCell SureL...
Catalog: ZA10001
Size: 1 set

Pippin Prep™ System (includes instrument and monitor)

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing

The Pippin Prep™ System is a small tabletop instrument that automates the size selection and collection of DNA for PGM™ sequencer libraries. Simply enter the desired base-pair target range via the software interface and after a 1.5 hr run the system will yield the programmed DNA fractions with virtually no low molecular weight contamination. The tight fragment size distributions obtained provide a more uniform template for amplification. And the processing of samples is simplified through the use of disposable pre-cast agarose gel cassettes. Benefits of the Pippin Prep™ System • Time savings c...
Catalog: 4471271
Size: 1 each

mMESSAGE mMACHINE® T7 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA; up to 39 µg of RNA per reaction • Extra convenience; cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped...
Catalog: AM1344M
Size: 25 rxns w⁄ Manual

Silencer ® Select Negative Control No. 1 siRNA

Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Negative Controls

Ambion® validated GAPDH Positive Control siRNA and non-targeting Negative Control siRNAs with the same chemical modifications for enhanced efficacy as in other Silencer ® Select siRNAs. • Validated siRNA controls for optimizing siRNA experiments • GAPDH Positive Control functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs? Silencer ®Select siRNAs are the next ...
Catalog: 4390843
Size: 5 nmol

pcDNA™3.1/His A, B, & C

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V385-20
Size: 20 µg

Gateway® pDEST™15 Vector

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Bacterial Expression Vectors

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11802-014
Size: 6 µg

2 ml Microcentrifuge Tubes with Screw Caps

Category: Products & Services > Instruments, Software, & Accessories

Catalog: 4305936
Size: 500 tubes

SuperScript® Double-Stranded cDNA Synthesis Kit

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

The SuperScript® Double-Stranded cDNA Synthesis Kit contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality double-stranded cDNA from total RNA or poly A + selected RNA (mRNA). The advanced capabilities of SuperScript® II Reverse Transcriptase, with its reduced RNase H activity, maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis. Features of the SuperScript® Double-Stranded cDNA Synthesis Kit include: • Reliability —Each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologie...
Catalog: 11917-010
Size: 10 reactions

Alexa Fluor® 488 C 5 Maleimide

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels

Conjugates prepared with the thiol-reactive Alexa Fluor 488 C 5 maleimide are significantly brighter and more photostable than fluorescein. The bright, green fluorescence of the Alexa Fluor 488 dye, which is spectrally similar to that of fluorescein, is pH independent from pH 4 to 10.
Catalog: A-10254
Size: 1 mg

Rabbit Anti-Goat IgG - HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Immunogen: Goat IgG Specificity: Goat IgG Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, Western blotting
Catalog: 61-1620
Size: 1.5 mg

TaqMan® Rodent GAPDH Control Reagents

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Applied Biosystems® TaqMan® Rodent GAPDH Control Reagents provide the components for using rodent GAPDH as a normalization control in applications with rat, mouse, and Chinese hamster cells. • Pre-designed primers and TaqMan® probe eliminate assay design • Rapid assay development guidelines can minimize optimization time • Separate components provide flexibility in assay set-up Comprehensive Guidelines Included Applied Biosystems® has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems® Real-Time PCR reagents and instrumentation. These guidelines are...
Catalog: 4308313
Size: 1000 reactions

pcDNA™3.1/Zeo (-)

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

This pcDNA™3.1/Zeo (-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a reverse-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+) or ...
Catalog: V865-20
Size: 20 µg

One Shot® OmniMAX™ 2 T1 R

Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells

The OmniMAX™ 2 T1 Phage-Resistant (T1 R ) E. coli strain is an improved chemically competent cell line, perfect for use in all cloning applications, including the Gateway® Technology. OmniMAX™ 2 -T1 R cells offer the highest transformation efficiency of any chemically competent cells in a One Shot® format with >5 x 10 9 transformants/µg pUC19. They also provide efficient transformation of highly methylated DNA, since OmniMAX™ 2 -T1 R cells lack the E. coli K12 restriction systems (mc rA Δ (mrr hs dRMS- mc rBC)). In addition, the strain carries the to nA genotype, which confers resistance to T1...
Catalog: C8540-03
Size: 20 transformations

Quant-iT™ PicoGreen® dsDNA Reagent

Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits

Using the PicoGreen® dsDNA quantitation assay, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides (1). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. PicoGreen® dsDNA Quantitation Reagents are: Orders of magnitude more sensitive than UV absorbance readings, saving on precious sample (Figure 1) Specific for dsDNA in the presence of equimolar amounts of RNA (Figur...
Catalog: P7581
Size: 1 ml

One Shot® BL21(DE3)pLysE Chemically Competent E. coli

Category: Products & Services > Competent Cells & Strains > Expression Competent Cells

BL21(DE3)pLysE E. coli are ideal for use with T7 promoter-based expression vectors. Like BL21(DE3)pLysS E. coli , BL21(DE3)pLysE carry the lambda DE3 lysogen. In addition, BL21(DE3)pLysE cells contain the pLysE plasmid, which constitutively expresses T7 lysozyme. T7 lysozyme reduces the basal expression of target genes by inhibiting T7 RNA polymerase. The pLysE plasmid in BL21(DE3)pLysE expresses higher levels of T7 lysozyme than the pLysS plasmid in BL21(DE3)pLysS. The BL21(DE3)pLysE strain provides tighter control of T7 RNA polymerase, which is necessary when the recombinant protein to be ex...
Catalog: C6565-03
Size: 20 × 50 µl

NuPAGE® Transfer Buffer (20X) (125 ml)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Transfer Buffers

NuPAGE® Transfer Buffer (20X) (125 ml) is a buffer used to transfer proteins from NuPAGE® gels to membranes for western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use. Related Link • Learn more about other Invitrogen products for your protein transfer needs .
Catalog: NP0006
Size: 125 ml

Recombinant Protein G - Sepharose® 4B

Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Media & Standards

Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose® 4B. It is supplied as a 50% suspension containing 25 ml rec-Protein G-Sepharose® 4B + 25 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin preservative). Applications: Immunoglobulin affinity purification and Immunoprecipitation. Recombinant protein G binds to the Fc region of many types of immunoglobulin G including: all four subclasses of human IgG mouse IgG rabb...
Catalog: 10-1243
Size: 25 ml

pcDNA™3.1/Zeo (+)

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

This pcDNA™3.1/Zeo (+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a forward-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+) or ...
Catalog: V860-20
Size: 20 µg

Gateway® pDEST™14 Vector

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Bacterial Expression Vectors

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11801-016
Size: 6 µg

BSA-Acetylated (20 mg/ml) (25 mg Tube)

Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides

Ambion® Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is essential. Six tubes of 25 mg are provided, at a concentration of 40–50 mg/mL. The acetylation process inactivates any trace nuclease activity that might be present. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is routinely added to restriction digests. The molecular weight is ~66 kD. Acetylated BSA is rigorously tested for contaminating nonspecific endonuclease, exonucleas...
Catalog: AM2614
Size: 6 tubes

Corvaris™ S220 System

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing

The Covaris™ S220 System is a required sample preparation instrument for use in the SOLiD™ System workflow. The instrument is an essential part of the emulsion PCR process used to prepare the beads for emulsion PCR. The Covaris™ System is used to shear DNA into 60 bp fragments for fragment library preparation. The Covaris™ S220 System includes a Covaris™ S220 instrument, laptop, and a recirculating chiller
Catalog: 4465653
Size: 1 instrument

Ion Proton™ System

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Semiconductor Sequencing Instruments

The Ion Proton™ System is the first benchtop sequencing system capable of human-scale genome, exome, or transcriptome sequencing in a few hours—with DNA-to-variants called in a single day. The system combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data. By leveraging the exponential improvements in the semiconductor industry (known as Moore’s Law), the Ion Proton™ Sequencing System provides an unprecedented level of scalability and flexibility to support a broad range of high throughput sequencing applications, rang...
Catalog: 4476610
Size: 1 system

TaqMan® Reverse Transcription Reagents

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

This kit provides the indivdual components to perform reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and sequence-specific reverse primers provide flexibility for cDNA synthesis. • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT)16 provide flexibility for cDNA synthesis • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions Life Technologies has developed a comprehensive set of guidelines to ensure success when using Rea...
Catalog: N8080234
Size: 200 reactions

Illumina® TotalPrep™ RNA Amplification Kit

Category: Products & Services > Kits & Assays > RNA Amplification Kits

The Ambion® Illumina® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The new TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous Illumina® RNA Amplification Kit. Experience these Improvements: • Improved first-strand cDNA synthesis using ArrayScript™ reverse transcriptase • Biotinylated UTP allows ease of use and optimal labeling • Maximization of cRNA yield using MEGAscript® IVT technology • Signific...
Catalog: AMIL1791
Size: 24 reactions

Plasmid pBR322

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > DNA Subcloning Vectors

Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B™ E. coli , also has 14 non-selectable unique restriction endonuclease recognition sites. Unique cleavage sites for Hin d III and Cla I are found in the promoter of the tetracycline resistance gene. Insertion of DNA at either of these two sites usually results in loss of tetracycline resistance. Performance and Quality Testing: Purity, DNA structure, and selected restriction endonuclease fragmentation...
Catalog: 15367-014
Size: 25 µg

pcDNA™4/V5-His A, B, & C

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V861-20
Size: 20 µg

Macrophage-SFM, (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Other Serum-Free & Specialty Media

Macrophage-SFM is a serum-free medium designed for the culture of human peripheral blood monocytes and macrophages (1). Contains L-glutamine, but no antibiotics.
Catalog: 12065-074
Size: 500 ml

DNA Zap ™ (250 ml each) with Manual

Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > Nuclease Control Reagents

Ambion® DNA Zap ™ (patent pending) consists of two solutions that are innocuous when used alone, but become a potent nucleic acid degrading solution when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. 250 mL of each solution are provided. • Completely degrades contaminating DNA and RNA at the level of PCR sensitivity • Works on contact • Degrades nucleic acid, unlike other products on the market that only act as detergents • Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tu...
Catalog: AM9890M
Size: 250 ml (Manual)

Calcium Phosphate Transfection Kit

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

Invitrogen's Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation.
Catalog: K2780-01
Size: 75 reactions

Protein A-HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Detection Kits & Reagents > Protein A

Protein A from Staphylococcus aureus, Cowan I Strain conjugated with highly purified horseradish peroxidase (RZ > 3.0). The product is liquid containing 10 mM phosphate, 0.15 M NaCl buffer, pH 7.4, 1% ovalbumin, 40% glycerol, and 0.1% proclin as preservative.
Catalog: 10-1023
Size: 3 mg

NuPAGE® Sample Reducing Agent (10X) (10 ml)

Category: Products & Services > Buffers & Chemicals > Reducing Agents

NuPAGE® Reducing Agent (10X) (10 ml) is used to reduce protein samples for protein gel electrophoresis. NuPAGE® Reducing Agent contains 500 mM dithiothreitol (DTT) at a ready-to-use 10X concentration in a stabilized liquid form. Use NuPAGE® Sample Reducing Agent when running proteins on NuPAGE® Bis-Tris gels to maintain protein samples in a reduced state during electrophoresis. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
Catalog: NP0009
Size: 10 ml

Platinum Wire for the XCell SureLock® apparatus (12 inch x 2)

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Other Gel Electrophoresis Accessories

Replacement Parts for Electrophoresis Apparatus
Catalog: EI9022
Size: each

Ion Xpress™ Plus Fragment Library Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100...
Catalog: 4471269
Size: 10 reactions

Platinum® Pfx DNA Polymerase

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits

Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA Polymerase from Thermococcus species KOD with proofreading (3´a5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic hot-start method that improves PCR specificity (2,3) (Figure 1). PCR x Enhancer Solution is included for higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of Platinum® Pfx DNA Polymera...
Catalog: 11708-013
Size: 100 reactions

2-YT Broth

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media

Application(s): For growth of hosts for replication of M13 vectors. Instructions for use: 1. Suspend 31 g in 1 L of demineralized water. 2. Autoclave for 15 min at 121°C.
Catalog: 22712-020
Size: 500 g

YPD Broth, Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Yeast Media

Gibco® YPD Broth is a ready-to-use liquid growth media used for yeast culture. Our filter-sterilized Gibco® YPD Broth can be used for nearly all research applications that require a liquid yeast culturing step such as protein expression, cloning, and expansion. • Gibco® microbiological media • Convenient ready-to-use liquid format • 1 L size available YPD Broth Recipe YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris ( P. pastoris ) and Saccharomyces cerevisae ( S. cerevisae ) yeast strains. Formulation per One Liter: 10 g Yeast extract 20 g Bac...
Catalog: A13745-01
Size: 1 L

I-Block™ Protein-Based Blocking Reagent

Category: Products & Services > Buffers & Chemicals > Immunoassay Buffers & Reagents > ELISA Buffers & Reagents

This Casein-based blocking reagent can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems. For Research Use Only. Not for use in diagnostics procedures.
Catalog: T2015
Size: 30 g

Sodium Pyruvate 100 mM Solution

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Sodium Pyruvate

Sodium Pyruvate is commonly added to cell culture medium as an energy source in addition to glucose. Since the cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not necessary to supplement all cultures. However, if the cells have been grown in medium supplemented with sodium pyruvate, we recommend the supplementation is continued as the cells become accustomed to this addition and their growth will lag without it. Gibco® Sodium Pyruvate, 100 mM Solution is formulated as 11 g per liter of water. The final concentration of sodium pyruvate used in most cell...
Catalog: 11360-070
Size: 100 ml

Champion™ pET SUMO Expression System

Category: Products & Services > Kits & Assays > Protein Expression Kits > Bacterial Expression Kits

The Champion™ pET SUMO Expression System produces the highest levels of soluble protein in E. coli . It utilizes a small ubiquitin-related modifier (SUMO) fusion, belonging to the growing family of ubiquitin-related proteins, to enhance the solubility of expressed fusion proteins. In contrast to ubiquitin, SUMO is involved in the stabilization and localization of proteins in vivo . After expression, the 11 kd SUMO moiety can be cleaved by the highly specific and active SUMO (ULP-1) protease at the carboxyl terminal, producing a native protein*. The Champion™ pET SUMO Protein and Peptide Expres...
Catalog: K300-01
Size: 20 reactions

Alexa Fluor® 488 Protein Labeling Kit

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits

Molecular Probes® Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, FISH, and more. Kits are available in 12 Alexa Fluor® dye colors, biotin, the hapten Oregon Green® 488, fluorescein EX, and Texas Red® dye. Each kit provides the components needed to perform three protein conjugations and purifications. Important Features of Protein La...
Catalog: A-10235
Size: 1 kit

pENTR™ 4 Dual Selection

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Gateway® Entry Vectors - pENTR

Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryotic systems • Ribosome bindi...
Catalog: A10465
Size: 10 µg