Products from Acridine Orange, 1 G to Normal Rat Serum
Acridine Orange is a cell-permeant nucleic acid binding dye that emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This unique characteristic makes acridine orange useful for cell-cycle studies. Acridine orange has also been used as a lysosomal dye. This dye is available in solution (A-3568).
Catalog: A1301
Size: 1 g
Size: 1 g
ATP is an essential component for kinase assays using LanthaScreen™ Technology. Invitrogen's ATP is 10mM in water.
Catalog: PV3227
Size: 500 µl
Size: 500 µl
Our highly purified lucigenin is a fluorescent Cl- indicator that has excitation/emission maxima of 368/505 nm and whose fluorescence is quenched in the presence of Cl-. The dye is useful for determining Cl- concentrations in liposomes, reconstituted membranes or in extracellular medium but is not useful for intracellular measurements.
Catalog: L6868
Size: 10 mg
Size: 10 mg
Ion PGM™ Enrichment Beads are for use with Ion PGM™ template preparation kits for enrichment of template-positive Ion Sphere™Particles. The Ion PGM™ Enrichment Beads provide you with: • Simple enrichment for template-positive Ion Sphere™ Particles • Dynabeads® technology • Support for Ion PGM™ System workflow • Support for Ion OneTouch™ 200 Template Kit v2, Ion OneTouch™ Template Kit, Ion PGM™ 200 Xpress™ Template Kit, and Ion Xpress™ Template Kit The Ion PGM™ Enrichment Beads are streptavidin-coupled Dynabeads®, the gold standard for isolation due to their high binding affinity and uniformity...
Catalog: 4478525
Size: 10 reactions
Size: 10 reactions
AB-TUBE-ANTI--MYC
Catalog: 460603
Size: 1 each
Size: 1 each
Gibco® Distilled Water is high quality water for use as a solvent in the preparation of cell culture media and laboratory reagents. Available in many sizes to meet your needs, Gibco® Distilled Water features: • Cell culture quality • Proven performance • Use in cell culture and molecular biology applications Cell culture quality Gibco® Distilled Water is prepared by distillation and filtration (0.1 µm), with no added substances. Life Technologies monitors the water quality on a routine basis. Conductivity, total organic carbon, endotoxin and bioburden are tested daily; metals are tested monthl...
Catalog: 15230-071
Size: 100 ml
Size: 100 ml
The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein. Free your lab from the time-consuming, labor-intensive process of seeding, feeding, passing, and maintaining multiple flasks of adherent cells (Figure 1). The FreeStyle™ 293 Expression System combines GIBCO® FreeStyle™ 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. FreeStyle™ 293-F cells and 293fectin™ are available as part of the Expression System or sep...
Catalog: K9000-01
Size: 1 kit
Size: 1 kit
VP-SFM AGT™ is a serum free, ultra low protein medium (5 µg/ml) in a new easy-to-use granular format. VP-SFM AGT™ is designed for the growth of VERO cells for virus production. It is also suitable for the growth of COS-7, MDCK, BHK-21 and Hep2 cells and for recombinant protein production.
Catalog: 12559-019
Size: 10 L
Size: 10 L
The ARCTURUS® Paradise® PLUS FFPE RNA Isolation Kit permits unprecedented gene expression analysis of millions of previously inaccessible samples by unlocking RNA from formalin-fixed, paraffin-embedded (FFPE) tissues. Amplify as little as 5 ng of the isolated fixed RNA using reagents and methods that have been specially optimized to overcome the challenges of cross-linked FFPE templates (Figure 1). Secure Downstream Success with the Paradise® QC Kit Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and th...
Catalog: KIT0312-I
Size: 12 samples
Size: 12 samples
PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can also be activated by phospholipids.
Catalog: PV3790
Size: 10 µg
Size: 10 µg
The FluoReporter® FITC Protein Labeling Kit provides a convenient and flexible means for attaching fluorescein (FITC, Ex/Em ~494/518 nm) to your monoclonal or polyclonal antibody (or any protein >30 kDa). The kit supplies the reagents needed for 5 to 10 labeling and separation reactions. Important Features of the FluoReporter® FITC Protein Labeling Kit: • Labeled proteins typically ready to use in under 3 hours with very little hands-on time • Optimized for 0.2–2.0 mg of protein with molecular weight >30 kDa • Labeled proteins are purified using convenient spin filters • Requires the antibody ...
Catalog: F-6434
Size: 1 kit
Size: 1 kit
GPX1 rabbit monoclonal antibody recognizes human glutathione peroxidase 1 (GPX1) in western blot and immunoprecipitation applications. Antibody Specifications Applications: Western blot, immunoprecipitation Species Reactivity: Human Clone ID: C8C4 Host Species and Isotype: Rabbit IgG
Catalog: A14011
Size: 100 µl
Size: 100 µl
Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV (1). The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb (Figure 2). AMV RT has endoribonuclease H activity. • Flexibility —Works with oligo(dT), random primers, or a gene-specific prim...
Catalog: 12328-019
Size: 750 units
Size: 750 units
An excellent tool for highly predictable and consistent extraction and isolation of nucleic acids from biological samples, following a simple magnetic separation protocol. Please note that this is a stand-alone product containing only Dynabeads® in a storage buffer. Our products Dynabeads® SILANE genomic DNA (Cat.no. 370-12D) or Dynabeads® SILANE viral NA (Cat.no. 370-11D) include the appropriate buffers and protocols you need to extract genomic DNA or viral nucleic acids respectively. Magnetic particles from alternative suppliers often have a random size range distribution, surface area and b...
Catalog: 37002D
Size: 5 ml
Size: 5 ml
The highest-quality RNA available from Ambion®, it is DNase-treated, subjected to unsurpassed quality control standards, and certified to contain small RNAs (miRNA, siRNA, and snRNA). One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment ...
Catalog: AM7960
Size: 100 µg
Size: 100 µg
Library Efficiency® DH5α™ Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 10 8 cfu/ µg plasmid DNA. Library Efficiency® DH5α™ Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal Maintains plasmid DNA with minimum recombination events Delivers high yield plasmid preparations for downstream applications M13mp cloning vectors may be propagated on a lawn of DH5α-FT™ , DH5αF...
Catalog: 18263-012
Size: 1 ml
Size: 1 ml
F4/80, Rat Anti-Mouse, (Biotin); Biotinylated Monoclonal antibody specific to Mouse F4/80. This antibody is validated for use in Flow Cytometry. Anti-F4/80 recognizes the expressed product of the Emr1 gene.
Catalog: MF48015
Size: 1 ml
Size: 1 ml
The Alexa Fluor 633 donkey anti—goat IgG is labeled with our bright, far-red—fluorescent Alexa Fluor 633 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from goat. To minimize crossreactivity, it has been adsorbed against rabbit, rat, mouse and human IgG. Fluorescence of this long-wavelength Alexa Fluor dye is not visible to the human eye but is readily detected by most imaging systems.
Catalog: A-21082
Size: 0.5 ml
Size: 0.5 ml
The Alexa Fluor 594 chicken anti—mouse IgG is labeled with our bright, red-fluorescent Alexa Fluor 594 dye (which is spectrally similar to Texas Red dye) and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. Chicken antibodies lack a classic "Fc" domain and will not bind to mammalian IgG Fc receptors. To minimize crossreactivity, the antibody has been adsorbed against human and rabbit IgG prior to conjugation.
Catalog: A-21201
Size: 0.5 ml
Size: 0.5 ml
The pcDNA™6 BioEase™ Gateway® Biotinylation System provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pcDNA™6/BioEase™-DEST vector (Figure 1) incorporates a 72 amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. This K. pneumoniae tag is recognized and efficiently biotinylated in vivo by a native mammalian system similar to the enzyme Biotin Protein Ligase (BPL) in E. coli (1). Proteins produced from the pcDNA™6/BioEase™-DEST vector are expressed as fusion proteins to this sequen...
Catalog: SA100-02
Size: 50 µl
Size: 50 µl
An improved formula for preparing denaturing formaldehyde/MOPS agarose gels. Formaldehyde is included. Gels made with this buffer are considerably less brittle than conventional formaldehyde gels, making handling of the gels much easier. One bottle containing 250 mL is provided. NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.
Catalog: AM8676
Size: 250 ml
Size: 250 ml
The cationic mitochondrial dye, 4-Di-2-ASP is a styryl with a large Stokes shift. It can also be used to stain presyntptic nerve terminals independent of neuronal activity.
Catalog: D-289
Size: 1 g
Size: 1 g
The Alexa Fluor® 568 goat anti-mouse IgG 1 is labeled with our bright, red-fluorescent Alexa Fluor® 568 dye (which is spectrally similar to Rhodamine Red™ dye) and is prepared from affinity-purified antibodies that react with the Fc portion of the heavy chain of mouse IgG 1 . To minimize crossreactivity, the antibody has been adsorbed against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG 2a , IgG 2b and IgG 3 prior to labeling.
Catalog: A-21124
Size: 250 µl
Size: 250 µl
Alexa Fluor 555 goat anti—rabbit IgG is labeled with our exceptionally bright, orange-fluorescent Alexa Fluor 555 dye (which is spectrally similar to Cy3 dye) and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize crossreactivity, it has been adsorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum. We also provide a version of the same antibody that has been cross-adsorbed against additional species (Cat. no. A-21429).
Catalog: A-21428
Size: 0.5 ml
Size: 0.5 ml
Immunogen: Human IgG Specificity: Human IgG (H+L) Reactivity: Human IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background....
Catalog: 62-7120
Size: 1 ml
Size: 1 ml
Applied Biosystems® MuLV Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. It can be used for the preparation of cDNA libraries, or for first strand synthesis in RT-PCR, or in dideoxy sequencing reactions in place of Klenow enzyme for G+C-rich regions. MuLV Reverse Transcriptase lacks endonuclease activity and has extremely low RNase H activity compared to AMV reverse transcriptase.
Catalog: N8080018
Size: 5000 units
Size: 5000 units
The Ambion® Single Tube Magnetic Stand accommodates one 1.5 mL microfuge tube. With this stand, as with any magnetic stand, centrifugation is replaced by the attraction between capture beads in a solution and the magnet in the stand, resulting in the beads collecting on one side of the microfuge tube. Thus, the captured species (e.g., mRNA) is quickly and efficiently separated from the solution's other components.
Catalog: AM10026
Size: 1 each
Size: 1 each
Highly purified water (at least ASTM Type III or ISO Grade 3) is required when using the M220 Focused-ultrasonicator™. One AFA-grade water bottle (800 ml) is sufficient for more than 50 uses.
Catalog: 4482280
Size: 1 each
Size: 1 each
A sterile set of solutions intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. The HKGS Kit provides, in separate vials, all the components of complete HKGS (S-001-5). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional.
Catalog: S-001-K
Size: 1 kit
Size: 1 kit
NativePAGE™ 5% G-250 Sample Additive is added to samples prepared with the NativePAGE™ Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE™ gels. This Additive is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.
Catalog: BN2004
Size: 0.5 ml
Size: 0.5 ml
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 51985-034
Size: 500 ml
Size: 500 ml
Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing This Trypsin is modified as follows: With Without • EDTA • Phenol Red
Catalog: 15400-054
Size: 100 ml
Size: 100 ml
Sera Category: Specialty Origin: New Zealand.
Catalog: 16210-064
Size: 100 ml
Size: 100 ml
Ham's F-12K (Kaighn's) medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) medium was developed for primary human hepatocytes, as well as some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) medium is manufactured as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 21127-022
Size: 500 ml
Size: 500 ml
BN/SDS 2D STARTER KIT
Catalog: BN1004SP
Size: 1 kit
Size: 1 kit
0.5-10 Kb RNA Ladder is suitable for sizing single-stranded RNA ranging from 0.5 kb to 10 kb in glyoxal or formaldehyde agarose gels. The ladder consists of nine bands in even increments (0.5, 1, 1.5, 2, 3, 4, 6, 8, and 10 kb) to simplify size estimation. The 1.5 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-200
Size: 75 µg
Size: 75 µg
NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. NuPAGE® Novex Tris-Acetate Midi-Gels (8 x 13 cm) can run up to 26 samples. The NuPAGE Midi-Gels run in the XCell4 Surelock™ Midi-Cell and, with a Midi Gel Adapter, can run in a Bio-Rad Criterion Cell. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity,...
Catalog: LA0050
Size: 1 kit
Size: 1 kit
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Catalog: 10710C
Size: 10 ml
Size: 10 ml
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