Products from Centri-Sep™ 96-Well Plates to Nupage® Novex® 7% Tris-Acetate Gel 1.0 Mm, 12 Well

Centri-Sep™ 96-Well Plates

Category: Products & Services > Columns, Resins & Plates > Spin Filters, Columns, & Purification Plates

Centri-Sep™ 96's hydrated matrix enables effective and reliable high-throughput removal of excess BigDye® terminators from DNA sequencing reactions, giving you clean sequencing reads with uniform signal intensity starting with the first base. The plate's 96-well format enables you to purify 96 samples simultaneously, either manually or by robotics, within 15 minutes. • Processed faster than ultrafiltration columns • Rapid and efficient separations • Buffer is not preselected • Convenient 20-100 µL sample size • Dry gel format • Room Temperature Storage • Microcentrifuge format Designed for Per...
Catalog: 4367821
Size: 50 plates

FGF-Basic Recombinant Mouse Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines

Recombinant Mouse Fibroblast Growth Factor Basic (also known as FGF Basic, bFGF, FGF 2, FGF-β or Heparin Binding Growth Factor) is a bioactive protein intended for use in cell culture applications. FGF-basic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. • High purity —no interference from other proteins or contaminants • High biological activity —more results with less...
Catalog: PMG0031
Size: 100 µg

Liver, cDNA from Human Fetal Normal Tissue

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > cDNAs & Libraries > cDNAs

In addition to the previously listed Gene Pool™ cDNA, the following selection of cDNA from adult human normal, tumor, or disease tissues as well as human fetal normal tissue is available. See Gene Pool™ cDNA for description and storage information. All Special Order Products are subject to tissue/tumor availability. Please call for information. Allow up to two weeks for delivery.
Catalog: D8880-01
Size: 20 reactions

IL1RA Human ELISA Kit

Category: Products & Services > Kits & Assays > ELISA Kits > ELISA

The IL-1RA ELISA Kit is designed to quantify protein levels of human interleukin-1 receptor antagonist (IL-1RA) in biological samples. IL-1RA is a cytokine which opposes the actions of IL-α and IL-1β by binding to IL-1 receptors.
Catalog: KAC1181
Size: 96 tests

TaqMan® OpenArray® Real-Time PCR Accessories Kit

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Real-Time PCR Instrument Accessories

This kit contains the necessary accessories to process 10 TaqMan OpenArray® Real-Time PCR plates. TaqMan® OpenArray® Real-Time PCR Plates deliver world-class TaqMan® Assays in a more flexible, high-throughput format. Five customizable plate formats allow you to select from over 61,000 inventoried TaqMan® Gene Expression Assays, which are pre-loaded onto the OpenArray® Plates. View the available formats (PDF). The OpenArray® Real-Time PCR System enables over 8,000 real-time PCR assays to be run simultaneously to generate 32,000+ data points per day. As part of our greener product alternatives w...
Catalog: 4453993
Size: 1 kit

KDR (VEGFR2) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Receptor Tyrosine Kinases

KDR (VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors.
Catalog: PV3660
Size: 10 µg

EIF2AK3 (PERK) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER.
Catalog: PV5106
Size: 10 µg

BMPR1A (ALK3) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

BMPR1A is a Type I member of the TGF beta receptor superfamily of transmembrane ser⁄thr kinases which phosphorylates intracellular SMADs in response to bone morphogenic proteins.
Catalog: PV6038
Size: 10 µg

InVision™ His-Tag In-Gel Stain

Category: Products & Services > Labeling & Detection Products > Gel & Membrane Stains

InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you'll: Stain proteins fused to an oligohistidine sequence Eliminate western blotting-detect His-tagged proteins directly in the gel Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner Visualize results in under 3 hours Convenienty provided as one ready-to-use solution, InVision™ His-tag ...
Catalog: LC6030
Size: 500 ml

TaqMan® Array Human Androgens

Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays

The Applied Biosystems® TaqMan® Array Human Androgens 96-well Plate contains 92 assays to androgen associated genes and 4 assays to candidate endogenous control genes. The panel of assays in the TaqMan® Array Human Androgens 96-well Plate targets genes which code for enzymes involved in androgen synthesis, regulators of androgen production, and receptors and signal transduction proteins which mediate their effects, and targets of androgen signaling. The panel also includes the Y-chromosome SRY genes, which play roles in male phenotype development, as well as differentiation of the bipotential ...
Catalog: 4414083
Size: 1 standard plate

TaqMan® Array Human Transcription of mRNA, Fast 96-Well Plate

Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Transcription of mRNA 96-well Plate contains 44 assays to transcription of mRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.
Catalog: 4418839
Size: 1 Fast plate

ElectroMAX™ DH10B™ T1 Phage-Resistant Competent Cells

Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells

ElectroMAX™ DH10B™ T1 Phage-Resistant electrocompetent E. coli cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 10 10 cfu/ µg plasmid DNA in efficiency. They are ideal for applications requiring high efficiency transformation, including cDNA and gDNA library construction. • Maximizes yield of transformants from limited cloning products • Permits efficient cloning of methylated DNA • Resistant to T1 and T5 bacteriophages • Supports blue/white screening by α-complementation on plates containing X-Gal or Bl...
Catalog: 12033-015
Size: 0.5 ml

Biotinylated Positive Control Oligo Probe (ALU)

Category: Products & Services > Controls, Standards & Ladders > DNA Control Probes

Applications: ISH
Catalog: Q151P.0100
Size: 0.5 ml

imMedia™ Growth Medium, agar, kanamycin

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media

imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need—including your choice of antibiotics and optional X-gal/IPTG—to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a...
Catalog: Q611-20
Size: 20 each

imMedia™ Growth Medium, agar, ampicillin

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media

imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need—including your choice of antibiotics and optional X-gal/IPTG—to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a...
Catalog: Q601-20
Size: 20 each

Streptavidin, Alexa Fluor® 488 Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin

Alexa Fluor® 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western ...
Catalog: S-32354
Size: 0.5 ml

IgG Goat Anti-Human pAb, PE Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

The IgG (γ), goat F(ab’), 2 R-phycoerythrin (R-PE) dye conjugate is a polyclonal goat antibody that is Fc specific and recognizes human IgG. R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence emission without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients. Use of R-PE in antibody conjugates results in probes with greatly enhanced detectability. Antibody Specifications Description: IgG (γ), Goat F(ab’)2 polyclonal, Fc specific Label: R-PE Validated Appl...
Catalog: H10104
Size: 0.5 ml

Expi293™ Expression Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > 293 Media

Expi293™ Expression Medium is a chemically-defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density cultures of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression. • Supports growth of suspension 293 cultures to densities of over 1.6 x 10 7 cells/mL, with >95% cell viability • Transfection-compatible serum-free suspension culture medium allowing transfection efficiencies of greater than 70% using ExpiFectamine...
Catalog: A1435101
Size: 1000 ml

4-Di-1-ASP (4-(4-(Dimethylamino)styryl) -N-Methylpyridinium Iodide)

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

DASPMI (4-Di-1-ASP) is a styryl dye stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane potential.
Catalog: D-288
Size: 1 g

RNA secure ™ Reagent

Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > Nuclease Control Reagents

Ambion® RNA secure ™ (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution. 10 mL supplied. • No post-treatment autoclaving • Safe, easy-to-use reagent inactivates RNases in solutions • Inactivation process can be repeated to protect against newly introduced contaminants • Compatible with downstream procedures, such as RT-PCR and in vitro transcription • Inactivate RNases in Tris and other solutions that cannot be treated with DEPC RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency ...
Catalog: AM7006
Size: 10 ml

Escherichia coli (K-12 strain) BioParticles®, BODIPY® FL Conjugate

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Phagocytosis Kits & Phagocytic Particles

The Molecular Probes® BioParticles® product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles® products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan ( S. cerevisiae ) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dy...
Catalog: E-2864
Size: 10 mg

Fluo-4, Pentapotassium Salt, cell impermeant

Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...
Catalog: F-14200
Size: 500 µg

OPCML Recombinant Human Protein, His Tag

Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides

Opioid Binding Protein / Cell Adhesion Molecule-Like Protein (OPCML), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human OPCML precursor (NP_002536.1) (Met 1-Asn 322) fused to a C-terminal polyhistidine tag. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, ...
Catalog: 10328-H08H-25
Size: 5 × 5 µg

Homogenizer

Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Isolation Accessories

The Homogenizer is used with the Micro-to-Midi™ Total RNA Purification System to reduce the viscosity of difficult tissue samples between 5 and 30 mg in RNA isolation procedures.
Catalog: 12183-026


Propidium Iodide - 1.0 mg⁄mL Solution in Water

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

Propidium iodide is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.
Catalog: P3566
Size: 10 ml

Advanced DMEM/F-12

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM⁄F-12 Media

Advanced DMEM / F-12 (Dulbecco's Modified Eagle Medium / Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM / F-12, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology. i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat. This Advanced DMEM / F-12 is manufactured as follows: With Without • Glucose • L-glutamine • Non Essential Amino Acids • HEPES • Sodium pyruvate • Phenol Red
Catalog: 12634-010
Size: 500 ml

5’ Phosphate labeled oligo

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

5’ Phosphate labeled oligo
Catalog: 4326342
Size: 80,000 pmol

7500 Fast System SDS v1.4 21 CFR Part 11 Module

Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software

The 21 CFR Part 11 Software Module for the 7500 Systems offers the flexibility of user-customizable security configuration settings to assist with 21 CFRp11 compliance. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4377355
Size: 1 kit

310 Genetic Analyzer (Factory Refurbished)

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sequencing & Genetic Analyzer Instruments

The 310 Genetic Analyzer is an automated single-capillary genetic analyzer designed for a wide range of sequencing and fragment analysis applications. • Perform comparative sequencing, linkage analysis, STR analysis, SNP detection, discovery and validation, mutation detection, and many other applications. • Eliminate gel preparation. • Set up runs in minutes. • Automate your workflow to minimize hands-on time and costs. • Detect five dyes simultaneously to increase throughput. Versatile, Affordable, Easy-to-Use, and Almost Hands-Free The 310 Genetic Analyzer – the ideal alternative to gel-base...
Catalog: 310-00-100-120WR
Size: 1 instrument

TrypLE™ Select (10X)

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > TrypLE™

Animal Origin Free
Catalog: 0040090DG
Size: 100 ml

ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit contains the MultiSite Gateway®-adapted ViraPower™ HiPerform™ promoterless lentiviral expression vector, pLenti6.4⁄R4R2⁄V5-DEST™ for easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any promoter of choice in dividing and non-dividing mammalian cells. The pLenti6.4⁄R4R2⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integras...
Catalog: A11146
Size: 20 reactions

Dynabeads® CD34 Positive Isolation Kit

Category: Products & Services > Beads & Microspheres > Dynabeads® for Cell Isolation & Expansion > Dynabeads® for Human Cell Isolation

Use this kit to isolate a high purity and yield of human CD34+ progenitor stem cells. Positively isolated cells are bead and antibody-free, phenotypically unaltered and suitable for any downstream applications including flow cytometry, functional studies, and cell culture to produce dendritic cells (DC's). The Dynal® CD34 Progenitor Cell Isolation System can also be used to isolate CD34+ cells from rhesus monkey (Macaca mulatta). Positive isolation of human CD34+ progenitor stem cells with bead release Stem cells can be isolated directly from whole blood, cord blood or bone marrow Isolated CD3...
Catalog: 11301D
Size: 5 ml

Mouse MDR1a Vesicles

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Drug Metabolizing Enzymes

Mouse MDR1a Vesicles are insect derived purified plasma membranes with inserted MDR1a transport protein (P-glycoprotein, ABCB1). Our Mouse MDR1a Vesicles can be used to evaluate test compounds and drug interactions with MDR1a in in vitro assays. MDR1a is an ATP-binding cassette (ABC) associated with multidrug resistance, including resistance to anti-cancer drugs. Use Mouse MDR1a Vesicles to: • Investigate the transporter interactions of your drug candidates • Assess potential for transporter-mediated drug-drug interactions • Obtain high quality results with a large signal to noise ratios Conce...
Catalog: GM0004
Size: 0.5 ml

NativePure™ pcDNA™ Gateway® Vector Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The NativePure™ Mammalian Affinity Purification Kit provides an easy-to-use, efficient method for purifying native protein complexes. NativePure™ pcDNA™ Gateway® vectors (Figure 1) are designed to allow expression of biotinylated recombinant fusion proteins in mammalian cells through N- or C- terminal fusion of your recombinant protein of interest to the capTEV™ Tag. The capTEV™ Tag consists of a BioEase™ in vivo biotinylation peptide, two Tobacco Etch Virus (TEV) protease recognition sites, and a 6XHis tag. The BioEase™ tag, a 72-amino acid sequence from K. pneumoniae , directs in vivo biotin...
Catalog: BN3002
Size: 1 kit

Tempus™ 12-Port RNA Isolation Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

This Applied Biosystems® product is designed for the purification of RNA from whole blood for real-time RT-PCR, microarrays, or other high-quality RNA experiments. 12 samples can be processed in about 45 minutes. For use with the ABI PRISM® 6100 Nucleic Acid PrepStation, sufficient reagents are included for 24 purifications. • Extract pure, intact RNA • Isolate more than 6 µg of RNA from 3 mL blood • Simple workflow—less time and fewer steps than other kits (see Figure) • Highly stable RNA, free of contaminating gDNA, and inhibitors; no DNase treatment required • Purified RNA used for the gene...
Catalog: 4378672
Size: 24 preps

Neon™ Transfection System 100µL Kit (50 Reactions)

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Electroporation Instrument Accessories

The Neon™ Transfection System 100 µl Kit is designed specifically for use with the Neon™ Transfection System device. Use this kit for transfection volumes of 100 µl, containing 5 × 10 5 – 2 × 10 6 adherent cells or 1 × 10 6 – 5 × 10 6 suspension cells. Cells that have been transfected using the 100 µl Neon™ Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol. This ready-to-use universal kit comes complete with enough buffer and tips to run up to 50 reactions (25 in duplicate) using optimized or user defined protocols. For Research...
Catalog: MPK10025
Size: 50 reactions

ChromaTide® Alexa Fluor® 594-5-dUTP

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides

Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis. ChromaTide® Labeled Nucleotides Specifications: • Ex/Em of dye: Alexa Fluor® 594-5-dUTP (590/615 nm) • L...
Catalog: C-11400
Size: 25 µl

Nix Mouse Monoclonal Antibody (clone ZN002)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

Ms anti-Nix; Unconjugated Monoclonal antibody specific to Human, Mouse, (Rat) Nix. This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-Nix recognizes the expressed product of the BNIP3L gene, also known as Bnip3l, Bnip3l.
Catalog: 39-3300
Size: 100 µg

PKA II beta [pS114] (Regulatory Domain) Rabbit Polyclonal Antibody

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

PKA REG 11BETA [pS114) PAb; Unconjugated Polyclonal antibody specific to Mouse, (Human, Rat) PKA IIβ [pS114] (regulatory domain). This antibody is validated for use in Western Blot. Anti-PKA IIβ [pS114] (regulatory domain) recognizes the expressed product of the PRKAR2B gene. Catalog number 44998G is same antibody as former catalog number 44998. 44998G contains 50% glycerol, which improves the stability and longevity of this antibody.
Catalog: 44-998G
Size: 10 blots

GeneArt® Cloning into pcDNA™3.1 (+)

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries

GeneArt® Cloning into pcDNA™3.1 (+)
Catalog: 854341DE
Size: 1 each

UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals

UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl. When mixtures are extracted with Phenol:Chloroform:Isoamyl Alcohol, proteins are denatured and collected in the organic phase or at the interphase, while nucleic acids remain in the aqueous phase. UltraPure™ Phenol:Chloroform:lsoamyl Alcohol contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated amber bottles. Performance and Quality Te...
Catalog: 15593-049
Size: 400 ml

Annexin V, FITC Conjugate (Replaces AnnexinV01, AnnexinV013, PHN1010, PHN1008)

Category: Products & Services > Labeling & Detection Products > BioConjugates > Annexin V

In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including this FITC annexin V conjugate. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.
Catalog: A13199
Size: 500 µl

PureLink® HiPure Plasmid Megaprep Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Plasmid DNA Purification Kits

Fast, simple protocols - Purify your plasmid DNA in less than 2 hours Labor-saving - No need for extra steps to remove endotoxin and other contaminants Megapreps and Gigapreps save time by utilizing vacuum-assisted cartridges PureLink® HiPure Plasmid Purification Systems are designed to isolate plasmid DNA of the highest purity (1) with a wide scale range (Table 1). Simple protocols an anion-exchange resin allow you to purify plasmid DNA (Figure 1) at a quality equivalent to two passes through cesium chloride gradients - the most rigorous method for DNA purification. In less than 2 hours, DNA ...
Catalog: K2100-08
Size: 4 preps

Guanosine 5'-Triphosphate, BODIPY® FL 2'-(or-3')- O -( N -(2-Aminoethyl) Urethane), Trisodium Salt (BODIPY® FL GTP)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides

BODIPY FL adenosine 5'-triphosphate (A-12410) and BODIPY FL guanosine 5'-triphosphate are composed of a BODIPY FL fluorophore attached to the 2' or 3' position of the ribose ring via a linker. Interactions between the fluorophore and the purine base are evident from the spectroscopic properties of these nucleotide analogs. The fluorescence quantum yield of BODIPY FL GTP is only about one-tenth that of BODIPY FL ATP; however, this yield may be enhanced upon binding of BODIPY FL GTP to GTP-binding proteins.
Catalog: G-12411
Size: 100 µl

TMB Solution (BLOT)

Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chromogens

TMB (Tetramethyl Benzidine) chromogen solution may be used with horseradish peroxidase (HRP)-based immunodetection procedures.
Catalog: 00-2019
Size: 500 ml

Cholera Toxin Subunit B (Recombinant), Alexa Fluor® 647 Conjugate

Category: Products & Services > Labeling & Detection Products > BioConjugates > Protein Conjugates

Molecular Probes® cholera toxin conjugates are made from a recombinant version of the B subunit only. This allows us to provide a very high-purity product that is completely free of the toxic A subunit. Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1 , making it a powerful tool for retrograde labeling of neurons. This tracer has been used in a variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall. When used in neuronal tracing applications, CT-B is typically introduc...
Catalog: C-34778
Size: 100 µg

DMEM, Low Glucose, Pyruvate

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • HEPES • L-Glutamine • Sodium Pyruvate • Phenol Red
Catalog: 11885-092
Size: 10 × 500 ml

Biotin Mouse Antibody (clone Z021), HRP Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Anti-Hapten Antibodies

Clone/PAD: Z021 Immunogen: Synthetic biotin Specificity: Binds strongly to biotinylated proteins. Reactivity: Biotinylated proteins Applications: ELISA, Cell and tissue staining
Catalog: 03-3720
Size: 1 ml

RediPlate™ 96 RiboGreen® RNA Quantitation Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits

The RediPlate™ 96 RiboGreen® RNA Quantitation Kit is a rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assay. The microplate has been pre-loaded with RiboGreen® Reagent, which shows bright green fluorescence upon binding to RNA or DNA (1). You simply add buffer and samples to the microplate wells, wait 10 minutes, and then read the fluorescence in any standard fluorescence-based microplate reader. The versatile RediPlate™ 96 design is ideal for either high-throughput quantitation or the measurement of a small number of samples. The RediPlate™ 96 RiboGreen® RNA Quantitat...
Catalog: R32700
Size: 1 kit

NuPAGE® Novex® 7% Tris-Acetate Gel 1.0 mm, 12 Well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > Tris-Acetate Gels

NuPAGE® Novex Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels...
Catalog: EA03552BOX
Size: 1 box