Products from Texas Red®-X Goat Anti-Rabbit Igg (H+L) to Champion™ Pet100 Directional Topo® Expression Kit with Bl21 Star™ (De3) One Shot® Chemically Competent E. Coli
The Texas Red-X goat anti—rabbit IgG is labeled with our bright, photostable, red-fluorescent dye (which contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and the antibody). The antibody is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize crossreactivity, it has been adsorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Catalog: T-6391
Size: 0.5 ml
Size: 0.5 ml
Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols. The solution contains 1% Ficoll (type 400), 1% polyvinylpyrrolidone, and 1% bovine serum albumin. Sterile filtered.
Catalog: 750018
Size: 100 ml
Size: 100 ml
Immunogen: DNP Specificity: Specific for DNP (2,4-Dinitrophenol). Reactivity: DNP Applications: ELISA
Catalog: 71-3500
Size: 100 µg
Size: 100 µg
Molecular biology grade, Ambion® SDS, 10% Solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. SDS is used as a component of both hybridization and wash buffers for northern and Southern blots and has many other uses around the lab.
Catalog: AM9823
Size: 1 L
Size: 1 L
Mouse monoclonal HP6069; specific for the Fc region of human IgG1. Replaces product number 053300.
Catalog: A-10630
Size: 250 µl
Size: 250 µl
The Qubit® (previously known as Quant-iT™) dsDNA BR Assay Kit is designed specifically for use with the Qubit® 2.0 Fluorometer . The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer. Common contaminants, such as salts...
Catalog: Q32850
Size: 100 assays
Size: 100 assays
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Catalog: 10510
Size: 10 ml
Size: 10 ml
The PureLink® Air Porous Tape is a component in the PureLink® 96 Plasmid Purification System. The PureLink® 96 Plasmid Purification offers a method for high throughput plasmid DNA isolation. The PureLink® Air Porous Tape allows for proper aeration of bacterial cultures.
Catalog: 12262-010
Size: 50 pieces
Size: 50 pieces
PCRII TOPO TA Cloning Kit
Catalog: 10351021
Size: 20 reactions
Size: 20 reactions
The E-Gel® Camera Hood Kit, when combined with a base, forms the E-Gel® Imager System, which is a personal imaging system for documenting and analyzing agarose gels and E-Gel® cassettes. Each E-Gel® Camera Hood Kit includes a compact digital camera mounted in a sleek hood, a universal filter, power and USB cables, and two powerful software applications. There are three bases available for purchase that will work with the E-Gel® Camera Hood Kit: a UV Light Base, a Blue Light Base, and an E-Gel® Adaptor Base. In any of these three configurations, the E-Gel® Imager system provides a small and lig...
Catalog: 4466601
Size: 1 kit
Size: 1 kit
The ViraPower™ HiPerform™ Lentiviral FastTiter™ TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti7.3/V5-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central ...
Catalog: K5320-00
Size: 1 kit
Size: 1 kit
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12562-013
Size: 5 transfections
Size: 5 transfections
The Ion 316™ Chip Kit contains chips for sequencing runs using the Personal Genome Machine™ System, the first PostLight™ semiconductor-based sequencer. The Ion 316™ Chip detects polymerase-driven base incorporation and translates this information into digital form. By eliminating the use of light-producing chemistry and optical systems, this revolutionary advance in next-generation sequencing technology makes massively parallel sequencing affordable to nearly every lab. Ion 316™ Chips Provide: • Easy handling and loading of templated Ion Spheres. • Over 6 million wells per chip. • Compatibilit...
Catalog: 4466616
Size: 4 pack
Size: 4 pack
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: V492-20
Size: 6 µg
Size: 6 µg
pTEF1/Zeo is a 3.6 kb vector designed to allow you to create your own cloning and/or expression vectors that confer resistance to Zeocin™ in both E. coli and yeast strains. The vector contains the Sh ble Zeocin™ resistance gene driven by the Saccharomyces cerevisiae TEF1 promoter for selection in any yeast. In addition, the synthetic EM-7 bacterial promoter is present for selection in E. col i. Several unique restriction sites flank the ZeoCassette™ region to allow easy subcloning of the region or cloning of additional elements into pTEF1/Zeo. This vector is also useful for generating gene dis...
Catalog: V503-20
Size: 20 µg
Size: 20 µg
Ambion® Silencer ® GAPDH Positive Control siRNA (human, mouse, rat) is ideal for developing and optimizing siRNA experiments. The control is validated for use in human, mouse, and rat cell lines. 40 nmol is provided. • Validated siRNA controls for optimizing siRNA experiments • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer ® Select...
Catalog: AM4631
Size: 40 nmol
Size: 40 nmol
This rat anti-E-Cadherin monoclonal antibody is specific to mouse E-Cadherin. E-Cadherin monoclonal antibody recognizes the expressed product of the Cdh1 gene. The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells. In addition, the tissue specificity of cadherin subtypes are becoming valuable markers for the identification and differential of certain cancers. Validated applications for E-Cadherin monoclonal antibody are epitope mapping and inhibition of E-Cadherin-dependent cell-cell contact. Applications: validated for epitope mapping and inhib...
Catalog: 13-1800
Size: 100 µg
Size: 100 µg
This product contains three boxes of 10 sets/box of iBlot® Transfer Stack, Mini, Nitrocellulose (Cat# IB3010-02) The iBlot® Dry Blotting System efficiently and reliably transfers proteins from polyacrylamide gels in seven minutes or less without the need for buffers or an external power supply. A self-contained unit (Figure 1), the iBlot® device uses disposable blotting stacks with integrated transfer membranes (nitrocellulose and PVDF), offering the convenience of a bufferless, plug-and-play system. Compared to conventional blotting techniques, the iBlot® system enables higher downstream dete...
Catalog: IB3010-32
Size: 3 x 10 sets⁄box
Size: 3 x 10 sets⁄box
KAPPA-OPIOID RECEPTOR PAb; Unconjugated Polyclonal antibody specific to Mouse, Rat κ-Opioid Receptor, internal. This antibody is validated for use in Western Blot. Anti-κ-Opioid Receptor, internal recognizes the expressed product of the OPRK1 gene. Catalog number 44302G is same antibody as former catalog number 44302. 44302G contains 50% glycerol, which improves the stability and longevity of this antibody.
Catalog: 44-302G
Size: 10 blots
Size: 10 blots
The red-orange-fluorescent BODIPY® 564/570 paclitaxel can be used to study microtubules in solution.
Catalog: P7501
Size: 10 µg
Size: 10 µg
The Attofluor cell chamber is a durable and practical coverslip holder designed for viewing live cell specimens on upright or inverted microscopes. Because the chamber is constructed from surgical stainless steel and is autoclavable, cells can be grown directly on coverslips within the unit. The O-ring seal design prevents samples from being contaminated by oil or leakage of media from the coverslips. The chamber accepts 25 mm round coverslips and mounts in a standard 35 mm diameter stage holder, and the 0.5 mm base dimension allows clearance for the objective when focusing. Spare O-rings for ...
Catalog: A-7816
Size: 3 each
Size: 3 each
Ambion® In Vivo siRNA Controls • Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts • Positive Control siRNAs functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability • For use in human, mouse, and rat cells What are Ambion® In Vivo siRNAs? Ambion® In Vivo siRNAs are designed using the proven Silencer ® Select algorithm and incorpora...
Catalog: 4457287
Size: 5 nmol
Size: 5 nmol
This dNTP Set consists of all four deoxyribonucleoside triphosphates (dATP, dCTP, dGTP, dTTP), each at a concentration of 100 mM. They are suitable for use in PCR, sequencing, nick translation, fill-in, cDNA synthesis, and TdT tailing reactions, as well as for dilution of radiolabeled dNTPs.
Catalog: 10297-018
Size: 4 × 25 µmol
Size: 4 × 25 µmol
The amine-reactive PyMPO succinimidyl ester can be used to create bioconjugates with this environment-sensitive fluorophore.
Catalog: S-6110
Size: 5 mg
Size: 5 mg
YEAST HOMOZYGOUS DIPLOID PLATE
Catalog: FL1002
Size: each
Size: each
Invitrogen makes blotting easier by providing a variety of pre-cut, pre-assembled membrane/filter-paper sandwiches. A protein's properties ( i.e. , charge, hydrophobicity, etc.) affect its ability to bind to membrane surfaces. Finding the right membrane may require experimenting with your specific protein on different membranes. Store all membranes at room temperature.
Catalog: LC2002
Size: 20⁄pack
Size: 20⁄pack
The Alexa Fluor 488 donkey anti—rabbit IgG is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. The donkey anti—rabbit IgG antibodies show minimum crossreactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins.
Catalog: A-21206
Size: 0.5 ml
Size: 0.5 ml
0.1-2 Kb RNA Ladder is suitable for sizing single-stranded RNA from 0.1 to 2 kb in glyoxal or formaldehyde agarose gels. The ladder consists of ten bands in even increments (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.8, 1.0, 1.5, and 2.0 kb) to simplify size estimation. The 0.3 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-100
Size: 75 µg
Size: 75 µg
Actin is an important tool for investigating cytoskeleton dynamics in-vivo . This highly purified actin from rabbit muscle is subjected to depolymerization and subsequent polymerization to ensure that the actin is able to assemble properly. The actin that polymerizes is then separated from remaining monomeric actin by centrifugation, depolymerized and packaged in monomeric form.
Catalog: A12375
Size: 1 mg
Size: 1 mg
Random Primers are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis. These primers are truly random and are suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates. To avoid storage buffer interference, Random Primers are supplied in a low-salt-concentration buffer. Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verif...
Catalog: 48190-011
Size: 9 units
Size: 9 units
UltraPure™ Agarose is a polysaccharide (see structure below) used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to > 20 kb. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved Packaging The new environmentally ...
Catalog: 16500100
Size: 100 g
Size: 100 g
Immunogen: Sheep IgG (H+L) Specificity: Sheep IgG (H+L) Applications: Flow cytometry, Immunofluorescence ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background. Enhanced sensitivity, higher titer, lower background -- try them t...
Catalog: 81-8611
Size: 1.5 mg
Size: 1.5 mg
DNase I, Amplification Grade digests single- and double-stranded DNA to oligodexyribonuleotides. The specific activity is >10,000 units/mg. DNase I, Amplification Grade is purified and tested for non-detectable levels of RNase contamination. Absence of RNase is tested by performing a Ribonuclease assay with RNA ladder. Application: Removing DNA from RNA (1) and protein preparations. Source: Purified from bovine pancreas. Performance and Quality Testing: Ribonuclease assay with RNA ladder; ability to digest single-stranded and double-stranded DNA to oligonucleotides determined. Unit Definition:...
Catalog: 18068-015
Size: 100 units
Size: 100 units
The Vybrant® DiD cell-labeling solution is a dye delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells for use in cell-cell fusion, cellular adhesion and migration applications.
Catalog: V-22887
Size: 1 ml
Size: 1 ml
Cells can naturally incorporate the thymidine analog BrdU into their cells during cell division, making this nucleoside analog an excellent marker of by cell cycle and proliferation. Analysis of incorporated BrdU can be with an anti-BrdU antibody.
Catalog: B23151
Size: 100 mg
Size: 100 mg
Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure Grade Hoechst 33342 is greater than or equal to 98% pure by HPLC.
Catalog: H21492
Size: 100 mg
Size: 100 mg
5´-T↓CTAGA-3´ 3´-AGATC↑T-5´ Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns. Concentration 8-20 U/ µL Contents Xba I 10X Buffer M (1mL) 10X Loading Buffer (1mL) Reaction Temperature 37°C Reaction Mixture Xba I 1 µL 10X Buffer M 2 µL 0.10% BSA 2 µL Substrate DNA Heat inactivation Enzyme inactivated by heating at 65°C for 20 minutes....
Catalog: 15226-012
Size: 2,000 units
Size: 2,000 units
This is a user guide for the ABPI PRISM 3100- Avant Genetic Analyzer. Important Note: 3100 System Sequencers will be retired at the end of 2011. Take advantage of our limited time promotion to either TRADE-IN your 3100 for a brand new 3500 or UPGRADE to a 3130. Please contact your local Sales Representative to learn more.
Catalog: 4333549
Size: 1 each
Size: 1 each
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 31985-070
Size: 500 ml
Size: 500 ml
This is the User Guide Manual for the AmpFLSTR® Identifiler® Plus PCR Amplification Kit. Note: For Forensic or Paternity Use Only.
Catalog: 4440211
Size: 1 manual
Size: 1 manual
Dulbecco's Modified Eagle Medium : Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM / F-12 is modified as follows: With • L-glutamine • HEPES • Phenol Red
Catalog: 11330-032
Size: 500 ml
Size: 500 ml
TC 100 Insect Medium was originally designed for the growth of insect cells, including Spodeptera frugiperda (Sf9), and the expression of recombinant Autographa Californica Nuclear Polyhedrosis Virus (rAcNPV) using the Baculovirus Expression Vector System (BEVS). Life Technologies offers a variety of Gibco® Media options for a range of insect cell culture applications . This IPL-41 Insect Medium is modified as follows: With • L-glutamine • Tryptose phosphate broth • Sodium bicarbonate
Catalog: 13055-025
Size: 500 ml
Size: 500 ml
The CellSensor® LEF/TCF-bla SW480 cell line contains a beta-lactamase reporter gene under control of the lymphoid enhancer factor/T cell factor (LEF/TCF) consensus binding sequence stably integrated into SW480 cells, which express a truncated form of the tumor suppressor protein adenomatous polyposis coli (APC). This cell line is a clonal population isolated by flow cytometry, with the pathway validated using RNAi against beta-catenin. The truncated, loss-of-function version of APC expressed in this cell line prevents the proper assembly of the beta-catenin destruction complex. This results in...
Catalog: K1675
Size: 1 vial
Size: 1 vial
The CellSensor® NFκB-bla RAW264.7 mouse macrophage cell line contains a beta-lactamase reporter gene under control of a stably integrated NFκB response element. This cell line is a clonal population isolated in response to lipopolysaccharide by flow cytometry. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, and has been validated for Zfi-factor and EC50 concentrations of the TLR7 ligand imiquimod. Additional data using alternate stimuli are shown. The molecules responsible f...
Catalog: K1673
Size: 1 vial
Size: 1 vial
The TaqMan® Human Immune Array from Applied Biosystems® is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of targets known to have implications in immune response. This product is designed with 96 TaqMan® Gene Expression Assays loaded in singletons into the array’s 384 wells and is compatible with an upgraded Applied Biosystems 7900HT Fast Real-Time PCR System. The targets include cytokines, chemokines, growth factors, immune regulators, apoptosis markers, ischemia markers, tissue-specific markers, and others including classic endogenous markers.This pro...
Catalog: 4370573
Size: 4 cards
Size: 4 cards
The Ion OneTouch™ 200 Template Kit v2 contains template preparation reagents for automated and reproducible template preparation for 200 base-read libraries using the Ion OneTouch™ System . As an integral component of the semiconductor sequencing workflow, the Ion OneTouch™ 200 Template Kit v2 is designed to make high-quality templated Ion Sphere™ particles using the Ion OneTouch™ System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery. The Ion OneTouch™ 200 Template Kit v2 provides you with: • Simple ...
Catalog: 4478316
Size: 10 reactions
Size: 10 reactions
The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...
Catalog: 4452136
Size: 1 kit
Size: 1 kit
Custom TaqMan® Gene Expression Assay, LG
Catalog: 4332079
Size: 2900 reactions
Size: 2900 reactions
DNA Grade Agarose has a high sieve capacity, providing optimal resolution of DNA during gel electrophoresis. The low sulfur content allows gels to be run faster without becoming brittle. Supplied as a powder that is reconstituted in 0.5X TBE for high-quality agarose gels.
Catalog: 75000-500
Size: 500 g
Size: 500 g
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K100-01
Size: 20 reactions
Size: 20 reactions
Products from Texas Red®-X Goat Anti-Rabbit Igg (H+L) to Champion™ Pet100 Directional Topo® Expression Kit with Bl21 Star™ (De3) One Shot® Chemically Competent E. Coli
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