Products from Mem, 2x Temin's, no Phenol Red to 6% DNA Retardation Gel 1.0 Mm, 10 Well

MEM, 2X Temin's, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Modified Eagle Medium (MEM)

Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • L-glutamine • HEPES • Earle's salts • Phenol Red • Sodium Bicarbonate
Catalog: 11935-046
Size: 500 ml

ROX Reference Dye

Category: Products & Services > Labeling & Detection Products > Fluorescent Dyes

ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR. This dye has been qualified for use on the ABI PRISM® 7700 with Platinum® Quantitative PCR SuperMix-UDG® , Platinum® Quantitative RT-PCR ThermoScript™ One-Step System, and SuperScript® III Platinum® One-Step qRT-PCR Kit. ROX Reference Dye: Normalizes non-PCR-related fluctuations in fluorescence Provides a stable baseline for multiplex quantitative PCR and RT-PCR Uses existing ROX spectral settings, eliminating the need to re-calibrate the thermal cycler
Catalog: 12223-012
Size: 500 µl

DMEM, High Glucose, no Glutamine, no methionine, no cystine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES • L-methio...
Catalog: 21013-024
Size: 500 ml

Penicillin-Streptomycin, Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics

The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibit...
Catalog: 15070-063
Size: 100 ml

Applied Biosystems® 7500 Fast Real-Time PCR System with Dell™ Notebook

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Real-Time PCR Instruments

The Applied Biosystems® 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. Life Technologies® supplies a Dell™ Notebook computer with the 7500 Fast System. • Specially designed Fast block ensures thermal uniformity at top speeds. • Faster ramp rates enable rapid results without compromising extension times or assay quality. • Fast optical plates ensure excellent precision in 10-30 ul reaction volumes. • Use TaqMan® Fast Universal PCR Master Mix to run multiple F...
Catalog: 4351106
Size: 1 system

CellSensor® Gli- bla NIH3T3 Cell Line

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits

The CellSensor® Gli-bla NIH 3T3 Cell Line contains a beta-lactamase reporter gene under control of the Gli response element stably integrated into NIH 3T3 cells. This cell line is a clonal population isolated by flow cytometry in response to the mouse sonic hedgehog (mShh) ligand. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, as well as validated for Z'-factor and EC50 concentrations of Shh. Additional data using known inhibitors or activators of the pathway have also been...
Catalog: K1642
Size: 1 vial

EdU (5-ethynyl-2’-deoxyuridine)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > BrdU, EdU & EU Reagents

EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a s...
Catalog: E10415
Size: 5 g

iBlot® Gel Transfer Device

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Blotting Systems

The iBlot® Gel Transfer Device performs western blotting transfer efficiently and reliably typically in 7 minutes or less without the need for liquid buffers. The device is integral part of the iBlot® Western Blotting System. The system consists of the device and disposable blotting stacks that contain the buffers and the transfer membrane (nitrocellulose or PVDF), and offers high quality transfer, convenience and speed. The device and the stacks are sold separately. The iBlot® system is ideal for scientists analyzing proteins through western blotting who want: • Complete protein transfer in s...
Catalog: IB1001
Size: 1 each

PichiaPink™ Secretion Signal Set

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Yeast Expression Vectors

The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene. The PichiaPink™ Secretion Signal Set is designed for use with the pPINK-HC and pPINK-LC vectors. Optimize Secretion of Your Expressed Protein By adding one of the 8 secretion signal sequences to your gene, you can secrete your protein into the media to make it easier to harvest and purify. Pichia pastoris secretes few native proteins, so the bulk of the protein in the media will be yours. Each DNA sequ...
Catalog: A11155
Size: 8 each

pSecTag/FRT/V5-His© TOPO® TA Expression Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Catalog: K6025-01
Size: 20 reactions

IL6 Mouse ELISA Kit

Category: Products & Services > Kits & Assays > ELISA Kits > ELISA

The Mouse IL-6 ELISA research use only kit is designed to detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader. Easy to run Rapid protocol Convenient precoated, removable 8-well strips Easy-to-Run Sandwich ELISA The total assay incubation time is only 3.5 hours. A monoclonal capture antibody specific for Mouse IL-6 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Mouse IL-6 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinyla...
Catalog: KMC0062
Size: 192 tests

DiSBAC 4 (3)

Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Voltage & Membrane Potential Sensors

Voltage Sensor Probes (VSPs) are a Fluorescence Resonance Energy Transfer (FRET)-based voltage-sensing assay technology used to measure changes in cellular membrane electrical potential. VSPs are ideal for screening compounds that modulate ion channels because they provide: High-throughput capabilities-screen 32,000 samples in one day Highly sensitive detection-accurately and precisely report a 1% change in the Emission Ratio per mV Rapid detection response-sub-second time response Ratiometric readout (the Emission Ratio)-reduces experimental errors arising from well-to-well variations in cell...
Catalog: K1022
Size: 5 mg

T7 RNA Polymerase

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Polymerases

T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available. Application: Synthesis of labeled and unlabeled RNA transcripts (1). Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid. Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction. Unit De...
Catalog: 18033-100
Size: 2 × 2,500 units

KLF4 Rabbit Polyclonal Antibody

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

Rb anti-KLF4; Unconjugated Polyclonal antibody specific to Human, (Mouse, Rat, Sw, Chicken, Bovine, Cp) KLF4 . This antibody is validated for use in Western Blot. Anti-KLF4 recognizes the expressed product of the KLF4 gene, also known as Klf4, Klf4.
Catalog: 42-4100
Size: 100 µg

TaqMan® Array Human Transcription of tRNA

Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays

The Applied Biosystems® TaqMan® Array Human Transcription of tRNA 96-well Plate contains 28 assays to transcription of tRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. • Affordable; m...
Catalog: 4414196
Size: 1 standard plate

Guanidine Isothiocyanate

Category: Products & Services > Buffers & Chemicals > Denaturing Agents

UltraPure™ Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations. It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas (1).
Catalog: 15535-016
Size: 500 g

Silencer ® KIF11 (Eg5) siRNA (Human, Mouse, Rat) (inc. 2 nmol Neg. Control siRNA)

Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls

Ambion® Silencer ® KIF11 (Eg5) Positive Control siRNA (Human, Mouse, Rat) is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs....
Catalog: AM4639
Size: 5 nmol

Alexa Fluor® 594 Donkey Anti-Goat IgG (H+L)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Alexa Fluor® 594 Donkey Anti-Goat IgG (H+L) is labeled with our superior, red-fluorescent Alexa Fluor 594 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from goat. To minimize cross-reactivity, it has been adsorbed against rabbit, rat, mouse, and human IgG.
Catalog: A-11058
Size: 0.5 ml

DNAzol® BD Reagent

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

DNAzol® BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes. • Specifically formulated for extraction of genomic DNA from whole blood • Optimized for the rapid Isolation of genomic DNA Efficient Isolation of Genomic DNA from Whole Blood DNAzol® BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol® BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. ...
Catalog: 10974-020
Size: 100 ml

TRIzol® Reagent

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

TRIzol® Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour. Key Features of TRIzol® Reagent: • Isolate RNA, DNA, and protein from the same sample • Superior lysis capability, even with difficult sample types • Optimized formulations and pro...
Catalog: 15596-026
Size: 100 ml

DS-31 Matrix Standard Kit (Dye Set D)

Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards

This kit contains 4 oligonucleotides respectively labeled with 6-FAM™, VIC®, NED™, and ROX™, pre-pooled in a single tube. Suitable for Dye Set D spectral calibration, on ABI PRISM® Analyzers: 3100-Avant™, 3100, and 3730. Supports 4-Dye fragment analysis applications (Linkage Mapping Set v2.5, Mouse Mapping Primers). The DS-31 Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing labeled DNA fragments on the Applied Biosystems® 3130 and 3100 Series Systems, 3700 DNA Analyzer, and 3730 Series Systems. The Data Collection Software for these instruments uses t...
Catalog: 4345829
Size: 8 runs

Novex® 8% Tris-Glycine Midi Gels 12+2 Well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > Tris-Glycine Gels

Novex® 8% Tris-Glycine Midi Gels (12+2 well) are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Each box contains 10 gels. • Individually packaged gels for optimal experiment design • Compatible with most protein standards • Usable for native and denaturing protein assays Choose the Right Gel Format for Your Experiments Novex® Tris-Glycine gels come in a variety of fixed concentrations from 4% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, ...
Catalog: WT0081BOX
Size: 1 box

Rabbit Anti-Mouse IgG2a - HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Immunogen: Mouse IgG2a Specificity: Mouse IgG2a. Exhibits no reactivity with other Mouse IgG subclasses, IgA, IgM, or light chains. Reactivity: Mouse Applications: ELISA, Immunohistochemistry (FFPE), Western blotting
Catalog: 61-0220
Size: 0.45 mg

Goat Anti-Mouse IgG (γ), HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Buffer: PBS No preservative
Catalog: M30107
Size: 1 ml

Goat Anti-Mouse IgG (H+L) - AP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Immunogen: Mouse IgG (H+L) Specificity: Mouse IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background. Enhanced sensitivity,...
Catalog: 62-6522
Size: 1 ml

Gibco® FreeStyle™ 293 Expression Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > 293 Media

FreeStyle™ 293 Expression Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features: • Complete and ready-to-use format containing GlutaMAX™ supplement • Ability to support suspension growth and transfection of 293-F cells • Animal origin-free, protein-free and chemically defined formulation • Easy use as part of the FreeStyle™ 293 ...
Catalog: 12338-018
Size: 1000 ml

RT-PCR Grade Water (10 x 1.5 mL)

Category: Products & Services > Buffers & Chemicals > Water

Ambion® RT-PCR grade water is tested for prokaryotic as well as eukaryotic genomic DNA contamination using an ultrasensitive PCR assay. It is provided in 10 x 1.5 mL. Prokaryotic DNA contamination is assessed by performing PCR with primers to 16S rRNA. Eukaryotic DNA contamination is assessed by performing PCR with primers to 18S rRNA. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity and is suitable for any PCR application.
Catalog: AM9935
Size: 10 × 1.5 ml

MAGPIX® Drive Fluid (4 pack)

Category: Products & Services > Buffers & Chemicals > Immunoassay Buffers & Reagents > Luminex® Buffers & Reagents

MAGPIX® Drive Fluid is the delivery medium for transporting the sample being analyzed on the Luminex® MAGPIX® system to the optic components. Each unit in the 4-pack of drive fluid contains 750 ml. Please refer to a Luminex® instrument user manual for proper installation and use of MAGPIX® Drive Fluid with the MAGPIX® System. For research use only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: MPXDF4PK
Size: 4 × 750 ml

Novex® Midi Empty Cassette Combs, 20 wells

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Other Gel Electrophoresis Accessories

Novex® Midi Empty Cassette Combs are designed to be used when pouring your own gels using Novex® Midi Empty Cassettes. Each comb has 20 wells and is 1 mm thick. For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Catalog: WC1020
Size: 1 pack

CD90/Thy-1 Rat Anti-Mouse mAb (clone G7), PE Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

The CD90/Thy-1 (clone G7) R-PE (phycoerythrin) conjugate is a monoclonal rat antibody that recognizes the Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A). R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thereby affording exceptional quantum yields and molar extinction coefficients. The use of PE in antibody conjugates provides probes with greatly enhanced detectability. Antibody Specifications Description: CD90/Thy-1, Rat IgG 2c monoclonal Label: R-PE (phycoer...
Catalog: A14726
Size: 25 µg

TrypLE™ Express (1X), Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > TrypLE™

TrypLE™ Express is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. Life Technologies offers a variety of TrypLE ™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Express is: Gentle on cells —maintains cell health for reproducible results Room-temperature stable —convenient to use Easy to use —substitutes directly into existing protocols, with no inactivation required Animal origin fre...
Catalog: 12605-010
Size: 100 ml

ViiA™ 7 System Block Upgrade Kit, Fast, 96-Well

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Real-Time PCR Instrument Accessories

This kit allows the ViiA™ 7 Real-Time PCR System to operate using a Fast 96-well block. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4453544
Size: 1 upgrade

GeneAmp® PCR System 9700 Dual 96-Well

Category: Products & Services > Instruments, Software, & Accessories > Instruments > PCR Machines (Thermal Cyclers)

The Applied Biosystems® GeneAmp® PCR System 9700 is specifically designed for reliable PCR. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The Dual 96-Well GeneAmp® PCR System 9700 is designed for use with 0.2 mL reaction tubes or 96-well reaction plates for high-throughput PCR, cloning, and cycle sequencing applications. • Request a Demo Key Highlights: • Compact design maximizes bench space • Two 96-well sample blocks for high-throughput applications • Interchangeable sample block modules for greater flexibility • Graphical user i...
Catalog: 4343176
Size: 1 instrument

Tango™ CCR5- bla U2OS Cells

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > GPCR Cell Based Assay Kits

For Academic pricing please login or contact us at discoverysciences@invitrogen.com. The Tango™ CCR5- bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 5 (CCR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR- bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase ( bla ) reporter gene under the control of a UAS response element. The Tango™ CCR5- bla U2OS cells are functionally validated for Z' and EC 50 concentrations of MIP-1a. In addition,...
Catalog: K1788
Size: 8 × 10^6 cells

Avadis® NGS Software (Academic)

Category: Products & Services > Instruments, Software, & Accessories > Software > Sequencing & Genetic Analysis Software

Avadis® NGS is a desktop software platform for analysis, visualization, and management of data generated by Next-Generation Sequencing (NGS) platforms. It supports workflows for Alignment, DNA-Seq, RNA-Seq, Small RNA-Seq and ChIP-Seq analysis and is designed with the biologist in mind. Alignment Avadis NGS supports an inbuilt alignment algorithm called COBWeb that supports the alignment of both short and long reads with an arbitrary number of gaps and mismatches. It also includes support for paired end reads and a variety of trimming options and screening databases. DNA-Seq The DNA-Seq workflo...
Catalog: 4467869
Size: 1 software

CD CHO Medium (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > CHO & DG44 Media

CD CHO medium is a protein-free, serum-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. With a proven track record of quality for more than 10 years, CD CHO medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates. When to Use CD CHO Medium • Your GS CHO cells are recommended to be grown in CD CHO medium • Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a reg...
Catalog: 10743-002
Size: 20 L

Dynabeads® CD4

Category: Products & Services > Beads & Microspheres > Dynabeads® for Cell Isolation & Expansion > Dynabeads® for Human Cell Isolation

Dynabeads® CD4 are magnetic beads that provide a robust and versatile tool to isolate or deplete human CD4 + T-cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. • Rapid isolation of CD4 + T-cells from any sample – no columns required • Positive isolation for molecular downstream assays or efficient depletion of CD4 + T cells Excellent Yield, Purity, and Viability of Recovered Cells Dynabeads® CD4 are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of human CD4 + cells directly from any samp...
Catalog: 11145D
Size: 5 ml

pBAD202 Directional TOPO® Expressions Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Bacterial Expression Vectors

Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Catalog: K4202-01
Size: 20 reactions

EGFr Mouse Monclonal Antibody (clone 31G7), Concentrate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

This monoclonal antibody reacts with the extracellular domain of epidermal growth factor receptor (170 kDa). Clone 31G7 is useful in detecting the majority of squamous cell carcinomas that arise from both squamous epithelium and metaplastic squamous epithelium. Positive tissue: Breast carcinoma. Clone/PAD: 31G7 Immunogen: EGFr derived from A-431 cells Specificity: EGFr. No cross-reactivity observed with c-erbB-2. Reactivity: Human Applications: Immunohistochemistry (FFPE) Pretreatment: Epitope Retrieval: Not required , Enzyme Digestion: Required (Digest-All™ 3/Pepsin) Expected Staining Pattern...
Catalog: 28-0005
Size: 2 ml

LIVE/DEAD® Violet Viability/Vitality Kit, for 405 nm excitation

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Vitality Assay Kits

The LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. Calcein violet AM and aqua-fluorescent reactive dye are optimal dyes for this application; both dyes utilize the violet laser allowing other laser lines to be used for conventional fluorochromes.
Catalog: L34958
Size: 1 kit

pEF5/FRT/V5-DEST™ Gateway™ Vector

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: V6020-20
Size: 6 µg

HistoMouse- Plus Kit (AEC, Broad Spectrum)

Category: Products & Services > Kits & Assays > Cell Analysis Kits > IHC & ICC Kits

The HistoMouse-Plus kit is the 2nd generation HistoMouse kit for the detection of mouse primary antibodies on mouse tissue samples without causing background due to endogenous mouse Ig. Our patented BEAT™ technology (Blocking Endogenous Antibody Technology) is used to prevent the secondary antibody conjugate from reacting with endogenous mouse Ig. The LAB-SA detection method is used to amplify the signal. In addition, our HistoMouse-Plus kit includes the AEC Single Solution chromogen⁄ substrate system to create an intense red deposit around the antigen⁄antibody⁄enzyme complex in the sample. Re...
Catalog: 85-9541
Size: For 50 Slides

RKIP (C-Term) Mouse Monoclonal Antibody (clone 3E12D7)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

Mouse anti-RKIP (C-term); Unconjugated Monoclonal antibody specific to Human, Mouse RKIP (C-term) . This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-RKIP (C-term) recognizes the expressed product of the PEBP1 gene, also known as Pebp1.
Catalog: 37-2100
Size: 100 µg

NeuroTrace® BDA-10,000 Neuronal Tracer Kit

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

The NeuroTrace™ BDA-10,000 Neuronal Tracer Kit contains the neuronal tracer, biotin dextran amine (BDA), and the detection reagents, avidin-horseradish peroxidase (avidin-HRP) and diaminobenzedine (DAB) for subsequent visualization by light or electron microscopy.
Catalog: N-7167
Size: 1 kit

R-phycoerythrin F(ab')2 Fragment of Goat Anti-Mouse IgG (H+L)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Affinity purified F(ab')2 fragment of goat anti-mouse IgG conjugated to R-phycoerythrin. To minimize cross-reactivity, this antibody has been absorbed against human IgG and serum.
Catalog: A10543
Size: 250 µl

LysoSensor™ Yellow/Blue DND-160 - Special Packaging

Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains

LysoSensor™ Yellow/Blue DND-160 is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye produces blue fluorescence in neutral environments, but changes to yellow fluorescence in more acidic environments. The pK a of LysoSensor Yellow/Blue DND-160 is ~4.2.
Catalog: L-7545
Size: 20 × 50 µl

Breast Adenocarcinoma (MCF-7) Total RNA

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > Purified Total RNA

The highest-quality RNA available from Ambion®, it is DNase-treated and subjected to unsurpassed quality control standards. One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any ...
Catalog: AM7846
Size: 100 µg

Alexa Fluor® 488 Donkey Anti-Mouse IgG (H+L)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

The Alexa Fluor 488 donkey anti—mouse IgG is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. The donkey anti—mouse IgG antibodies show minimum crossreactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep serum proteins.
Catalog: A-21202
Size: 0.5 ml

Power SYBR® Green RNA-to-C T ™ 1-Step Kit (Mini-Pack)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qRT-PCR Kits

Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-C T ™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes: • AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA • An additive that red...
Catalog: 4391178
Size: 40 reactions

6% DNA Retardation Gel 1.0 mm, 10 Well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Polyacrylamide Gels

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Catalog: EC6365BOX
Size: 1 box