pTEF1/Zeo is a 3.6 kb vector designed to allow you to create your own cloning and/or expression vectors that confer resistance to Zeocin™ in both E. coli and yeast strains. The vector contains the Sh ble Zeocin™ resistance gene driven by the Saccharomyces cerevisiae TEF1 promoter for selection in any yeast. In addition, the synthetic EM-7 bacterial promoter is present for selection in E. col i. Several unique restriction sites flank the ZeoCassette™ region to allow easy subcloning of the region or cloning of additional elements into pTEF1/Zeo. This vector is also useful for generating gene dis...
Size: 20 µg
The Alexa Fluor 488 Oligonucleotide Amine Labeling Kit provides a simple method for labeling amine-modified oligonucleotides with our bright, photostable, green-fluorescent Alexa Fluor 488 dye (which is spectrally similar to fluorescein). Oligonucleotides labeled with this kit can be used as primers and as probes in hybridization experiments but should not be used for automated DNA sequencing since the Alexa Fluor dye in the kit is supplied as mixed isomers. The kits have been optimized for labeling reactions with 50 µg of a 5'-amine—modified oligonucleotide, 18 to 24 bases in length.
Size: 1 kit
Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Selectively stains F-actin • Outstanding fluorescence performance • Excitation/Emission: 495/518 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples Get Superior Results in Your Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Flu...
Size: 300 units
The TA Cloning® Kit Dual Promoter with pCR™II vector provides a quick, one-step cloning strategy for directly inserting a Taq -amplified PCR product into a plasmid vector. The T7 and Sp6 promoters of the pCR™II vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCR™II cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts. Features of the TA Cloning® Kit Dual Promoter: • Fast &...
Size: 40 reactions
This mouse monoclonal antibody recognizes human, rat, and bovine serine pyruvate aminotransferase (AGXT). Antibody Specifications Applications: Immunoprecipitation, Immunocytochemistry, In-Cell ELISA Species Cross-Reactivity: Human, Rat, Bovine Clone ID: 1F9BC7 Host Species and Isotype: Mouse IgG1, κ
Size: 100 µg
SKP2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. In tumor tissues, SKP2 is expressed widely, such as colon mucinous carcinoma, gastric adenocarcinoma, breast cancer, melanoma, prostate adenocarcinoma, with particularly higher expression on lung adenocarcinoma, lung squamous carcinoma, squamous carcinoma of cervix, endometrial carcinoma, ovary serous carcinoma and leiomyosarcoma. A recent study suggests that SKP2 plays a role in oncogenesis and is involved in the pathogenesis of lymphomas. Positive tissue: Breast ca...
Size: 0.5 ml
Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Size: 1 x 10L
The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Green neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid s...
Size: 1 each
• Reactivity -Human • Applications -Flow Cytometry, Functional Studies, Immunohistochemistry (Frozen)*, Immunoprecipitation*, Inhibition* • Host, Isotype -Mouse, IgG2a-κ • Clonality, Clone -mAb, 7G2 *Application tested by outside source. Ms anti-CD73; Unconjugated Monoclonal antibody specific to Human CD73 . This antibody is validated for use in Flow Cytometry, Functional Studies, Inhibition of E-cadherin-dependent cell-to-cell contact, Immunohistochemistry, Immunoprecipitation. Anti-CD73 recognizes the expressed product of the NT5E gene.
Size: 100 µg
It is a sequencing primer to sequence mammalian expression vectors having the BGH polyadenylation signal. Primer is supplied as 2 µg which equals 358 pMoles. The melting temperature is 54.8°C. The sequence of the primer is: 5'd[TAGAAGGCACAGTCGAGG]3'
Size: 2 µg
The EnzChek® Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme acitivty in solution. The assay measures lysozyme activity on Micrococcus lysodeikticus cell walls, which are labeled to such a degree with fluorescein that the fluorescence is quenched. Lysozyme action can relieve this quenching, yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.
Size: 1 kit
The Alexa Fluor® 488 goat anti-mouse IgG 1 is labeled with our exceptionally bright, photostable Alexa Fluor® 488 dye (which is spectrally similar to fluorescein) and is prepared from affinity-purified antibodies that react with the Fc portion of the heavy chain of mouse IgG 1 . To minimize crossreactivity, the antibody has been adsorbed against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG 2a , IgG 2b and IgG 3 prior to labeling.
Size: 250 µl
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Buffer: PBS No preservative
Size: 1 ml
Size: 5 mg
The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Size: 1 kit
GIBCO® Astrocyte Medium has been specially formulated to support the growth of primary human and rat astrocytes while retaining their phenotypes. The medium has been internally validated and optimized to provide an optimal balance between cell growth and the maintenance of cell viability, normal phenotype, and function. If more rapid proliferation is desired, addition of an appropriate amount of epidermal growth factor (EGF) is recommended, although cells may exhibit morphological and phenotypic changes. Astrocytes are a type of glial cells of the nervous system with a typical star shape. It h...
Size: 500 ml
This precision plate holder is for use with Applied Biosystems® 7300 real-time PCR systems (SN 273000878 and above), as well as 7500 real-time PCR systems (SN 275001330 and above). Customers with earlier serial numbers should contact Product Support .
Size: 1 each
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g. the presence of SDS or urea) • In the presence of metal chelating agents (e.g. EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization. Performance and Quality Testing: End...
Size: 5 ml
These are the same primers that are currently included in the RETROscript® Kit (SKU# AM1710). They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript® Kit.
Size: 80 µl
The TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction). • Compatible with both Fast and Standard mode thermal cycling conditions • Pre-mixed components stored at 2°–8°C significantly reduce assay setup time • Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays •...
Size: 400 reactions
GIBCO® Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or components of unknown composition. • Reduced cell clumping in suspension culture • Higher viable cell densities • Animal origin free (AOF) and chemically defined components • Convenient packaging with a larger size now available • Customization available to configure packaging and delivery Simple and...
Size: 20 ml
The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibit...
Size: 100 ml
The Applied Biosystems® 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. Life Technologies® supplies a Dell™ Notebook computer with the 7500 Fast System. • Specially designed Fast block ensures thermal uniformity at top speeds. • Faster ramp rates enable rapid results without compromising extension times or assay quality. • Fast optical plates ensure excellent precision in 10-30 ul reaction volumes. • Use TaqMan® Fast Universal PCR Master Mix to run multiple F...
Size: 1 system
Activation of the heat shock response⁄unfolded protein response (HSR⁄UPR) occurs in response to a diversity of chemical, environmental, and physiological stress conditions. Transcriptional regulation of the human HSR is mediated by a family of three heat shock transcription factors (HSFs), HSF-1, -2, and -4. Stress-induced activation of quiescent HSF monomers results in their trimerization and accumulation in the nucleus, wherein they bind to and upregulate transcription of target genes (e.g. molecular chaperones, certain proteases, and other stress response genes) harboring a heat shock eleme...
Size: 1 vial
Perform up to 2,000 PCR reactions with Applied Biosystems® TaqMan® PCR Core Reagent kit, 10-Pack. Applied Biosystems® TaqMan® PCR Core Reagents, 10-pack provides the individual components needed to perform up to 2,000 PCR reactions (50 µl each). Features of the TaqMan® PCR Core Reagent Kit: • Separate components, packaged in 10 tubes, provide maximum flexibility in assay setup • AmpliTaq Gold® DNA polymerase, a chemically modified form of AmpliTaq® DNA Polymerase, improves specificity and yield • AmpErase® UNG protects against subsequent re-amplifications from PCR products containing dU to min...
Size: 2000 reactions
A 1-year Extended Warranty for your StepOne™ Real-Time PCR System will add 1 year of coverage to the existing warranty. With this extended warranty, your StepOne™ Real-Time PCR System is covered by our Rapid Exchange Service* protection plan. Should you encounter an issue with your instrument, call technical support and talk to one of our Certified Senior Technical Specialists to get assistance in resolving most technical problems. In the event that your instrument needs service, simply repackage the defective instrument and return it to us – we will pay the shipping costs. We will then send a...
Size: 1 each
The pLenti6.2⁄V5-DEST™ Gateway® Vector is a Gateway®-adapted ViraPower™ II lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker. Advantages • Stable expression • Long-term experiments • High-throughput screening • Accurate titer of functional virus (using Blasticidin method) • Flexible and versatile Gateway® recombination cloning t...
Size: 6 µg
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Size: 20 reactions
This Ambion® kit is for the rapid, high-throughput purification of RNA from mammalian cells and tissue in 96-well plates. The kit combines an organic extraction with magnetic bead–based purification yielding extremely high-quality RNA suitable for the most stringent applications, such as microarray analysis. The kit includes sufficient reagents for 96 reactions. • Improve gene expression studies with consistent results from experiment to experiment • Combines TRI Reagent® with Ambion's superior magnetic bead-based technology • Streamlined RNA purification, without sacrificing RNA quality or qu...
Size: 96 purifications
The red-fluorescent tetramethylrhodamine (TAMRA) alkyne can be reacted with azides via a copper-catalyzed click reaction. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the ...
Size: 0.5 mg
This mouse anti-Luciferase monoclonal antibody is specific to mouse Luciferase protein. Luciferase is a 61 kD enzyme that catalyzes the reaction of luciferin to generate light after its activation by ATP and oxidative decarboxylation Validated applications for Luciferase monoclonal antibody are Western blotting, immunofluorescence, and immunohistochemistry (frozen tissue). Applications: validated Western blotting, immunofluorescence, and immunohistochemistry (frozen tissue) Host species and isotype: mouse IgG1 Clone ID of monoclonal antibody (mAb): CS 17 Reactivity: purified Luciferase in mous...
Size: 100 µg
The Champion™ pET-DEST42 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. The Champion™ pET-DEST42 destination vector offers: Bacteriophage T7 lac promoter for high-level expression O operator sequences for lac repressor binding to ensure tighter regulation of transcription pBR322 ori for minimal basal expression C-terminal V5 and...
Size: 6 µg
The CultureWell cell culture systems provide an integrated set-up for preparing cultured cells for staining and imaging. Each system uses medical-grade silicone gaskets preassembled with standard optical-quality coverslips in cell culture plates. The entire system is provided sterile and ready to use.
Size: 1 set
Ambion® RNase Cocktail™ is a mixture of two highly purified ribonucleases, RNase A (500 U/mL) and RNase T1 (20,000 U/mL), and is free of DNase and nicking activities. Use RNase Cocktail™ for all situations where it is desirable to degrade RNA, i.e. plasmid minipreps and ribonuclease protection assays. Supplied in five tubes containing 1 mL each. Digestion of RNA with RNase A alone leaves fragments of RNA that are large enough to be visible on agarose gels and precipitate in ethanol. RNase A cuts after C and U residues, and RNase T1 cuts after G residues. Consequently, the mixture of both enzym...
Size: 5 tubes
Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, first to fluorescein monogalactoside (FMG) and then to highly fluorescent fluorescein. Enzyme-mediated hydorlysis of FDG can be followed by the increase in either absorbance or fluorescence.
Size: 5 mg
Ambion® Silencer ® KIF11 (Eg5) Positive Control siRNA (Human, Mouse, Rat) is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs....
Size: 5 nmol
Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This protein G is conjugated with highly purified horseradish peroxidase (RZ > 3.0) and is in enyme conjugate stabilizer containing 0.1% proclin as preservative. Recombinant protein G binds to the Fc region of many types of immunoglobulin G including: all four subclasses of human IgG mouse IgG rabbit IgG guinea pig IgG rat IgG goat IgG sheep IgG cow IgG horse IgG Recombinant protein G does not bind to IgG from chicken or cat, or to human IgA, IgM, or serum albumin. It binds wea...
Size: 1 ml
FreeStyle™ 293 Expression Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features: • Complete and ready-to-use format containing GlutaMAX™ supplement • Ability to support suspension growth and transfection of 293-F cells • Animal origin-free, protein-free and chemically defined formulation • Easy use as part of the FreeStyle™ 293 ...
Size: 1000 ml
CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a K a of ~10 9 M -1 . This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.
Size: 1 mg
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Size: 10 ml
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 7.5 cm x 8.4 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to m...
Size: 50 sheets
TA PCRII DUAL PROMOTER
Size: 1 each
Size: 1 plate
EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a s...
Size: 5 g
AccuPrime™ Taq DNA Polymerase High Fidelity amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and proofreading enzyme, and AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime™ Taq DNA Polymerase High Fidelity you'll get: The highest specificity and yield for the most robust PCR amplification 9-fold higher fidelity than Taq DNA polymerase alone Mimimal optimization steps, even with non-optimized primer sets Efficient amplification of targets over a broad size range up...
Size: 1000 reactions
Enrich epithelial tumor cells directly from whole blood, bone marrow, or PBMC suspensions. Enriched tumor cells are lysed for mRNA isolation and RT-PCR amplification. The sensitivity of combined enrichment and RT-PCR detection can be as high as 1 tumor cell in 1 ml blood. Depletion of epithelial tumor cells from whole blood/bone marrow or PBMC. Positive isolation of epithelial tumor cells for downstream molecular analysis. No columns required Starting samples: Whole blood, bone marrow, PBMC, buffy coat. Number of cells processed: 200 ml whole blood or ~4 × 10 9 PBMC.
Size: 5 ml
Applied Biosystems® TaqMan® Copy Number Assays are designed for copy number analysis of copy number variations (CNVs) and smaller genomic regions in the human genome. The TaqMan® Copy Number Assays are a collection of over 1.6 million pre-designed assays with genome-wide coverage. Assay targets include genes, known CNVs, and extragenic?non-gene regions. CNV is an important polymorphism associated with diseases such as cancer, immune diseases, and neurological disorders as well as drug metabolism. TaqMan® Copy Number Assays combine TaqMan® Assay Chemistry with Applied Biosystems® real-time PCR ...
Size: 967 µl
The Mouse IL-6 ELISA research use only kit is designed to detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader. Easy to run Rapid protocol Convenient precoated, removable 8-well strips Easy-to-Run Sandwich ELISA The total assay incubation time is only 3.5 hours. A monoclonal capture antibody specific for Mouse IL-6 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Mouse IL-6 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinyla...
Size: 192 tests
Ambion® Silencer® Negative Control #2 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. 40 nmol is provided. • Nontargeting with limited sequence similarity to known genes • Validated for use in human, mouse, and rat cells • Functionally proven to have minimal effects on cell proliferation and viability • HPLC purified, duplexed, and ready to use Negative control siRNAs are essential for determining transfection effi...
Size: 40 nmol
The NCode™ VILO™ miRNA cDNA Synthesis Kit is part of a novel quantitative assay that provides flexibility, sensitivity, and specificity when quantitating miRNA expression. The system combines a carefully optimized polyadenylation reaction with the market-leading reverse transcriptase, SuperScript® III RT, in a universal first-strand cDNA synthesis reaction. Tried and tested SuperScript® III Reverse Transcriptase is formulated in an enhanced buffer system to provide you with the most reliable firststrand synthesis and higher cDNA yields, with sensitivity and accurate quantitation from 10pg to 1...
Size: 50 reactions