Products from Human Endothelial-Sfm to Captavidin™ Biotin-Binding Protein

Human Endothelial-SFM

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Other Serum-Free & Specialty Media

Human Endothelial-SFM is a basal serum-free growth medium developed to support the isolation and expansion of human umbilical venous and arterial endothelial cells (1–4). Medium requires supplementation with bFGF (20 ng/ml), EGF (10 ng/ml), and human plasma fibronectin (10 µg/ml). Contains L-glutamine.
Catalog: 11111-044
Size: 500 ml

PBS - Phosphate-Buffered Saline 10X, pH 7.4

Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS

Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminatin...
Catalog: AM9625
Size: 1 L

IMDM, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Iscove's (IMDM)

Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This IMDM is modified as follows: With Without • L-glutamine • Phenol Red • α-thioglycerol • 2-mercaptoethanol
Catalog: 21056-023
Size: 500 ml

Quantifiler® Human DNA Quantification Kit

Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Quantification Kits

This real-time PCR-based kit is designed for the quantification of amplifiable human DNA that is present in a sample, and has been validated for human identification applications. An internal PCR control is formulated into each reaction to identify samples that may contain PCR inhibitors. • Quantifies DNA from a wide variety of sources including buccal swabs, blood, semen, tissue, and other human DNA sources making this kit suitable for casework, databasing, and paternity labs. • Chemistry compatible with commonly used extraction technologies for easy integration into current lab workflow. • P...
Catalog: 4343895
Size: 400 reactions

E-PAGE™ Blotting Pads (Reusable), 8.6 cm x 13.5 cm

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Blotting, Drying & Staining Accessories

Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
Catalog: LC2101
Size: 4 pads

Silencer ® Select Human Kinase siRNA Library V4

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > RNAi Oligos > siRNA Libraries

For the first time, you can obtain the benefits of Ambion® Silencer ® Select siRNAs in a convenient pre-plated, library format. These siRNA collections enable medium and high throughput analysis of phenotypes resulting from inhibition of large sets of targets from specific functional classes. • Ready-to-ship collections of Ambion® Silencer ® Select siRNAs to popular human gene classes • Three individual siRNAs per target provided in a convenient 96-well format • Designed with an all new algorithm that yields better, more consistent knockdown • Novel chemical modifications reduce off-target eff...
Catalog: 4397918
Size: 1 set

KT5720 PKA Inhibitor

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzyme Inhibitors

KT5720 is a potent, cell permeable inhibitor of PKA, and is a valuable tool in studies delineating cAMP-dependent signaling pathways, including activation of the transcription factor CREB. Inhibition of PKC, PKG, and MLCK is negligible. KT5720 is prepared by a chemical modification of K-252a. Format/Formulation: White solid
Catalog: PHZ1141
Size: 0.1 mg

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337456
Size: 1000 reactions

TaqMan® Reverse Transcription Reagents (10-Pack)

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

This Applied Biosystems® kit provides the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, and oligo (dT) 16 , reverse primers are included for cDNA synthesis flexibility. Supplied as 2000 reactions in 10 tubes (200 reactions/tube). • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions TaqMan® Reverse Transcription Reagents provide all...
Catalog: 4304134
Size: 2000 reactions

Dynabeads® Sheep Anti-Rat IgG

Category: Products & Services > Beads & Microspheres > Dynabeads® for Cell Isolation & Expansion > Dynabeads® for Other Cell Isolation

Dynabeads® Sheep-anti Rat IgG binds monoclonal rat IgGs of your choice for specific cell isolation or depletion. All cells from all species except from rat can be isolated with this product, depending only on the specific antibody of choice. Especially well suited for cell isolation from mouse samples. Depletion: cells can be depleted from a sample Positive isolation: cells can be positively isolated from a sample for molecular applications Negative isolation: cells can be negatively isolated from a sample (i.e. spleen or lymph node cells) by adding an antibody cocktail of rat IgGs towards the...
Catalog: 11035
Size: 5 ml

pcDNA™3.1 (+)

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

This pcDNA™3.1 (+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+) or r...
Catalog: V790-20
Size: 20 µg

Bolt® Mini Gel Tank

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Boxes

The Bolt® Mini Gel Tank is compatible with Bolt® Bis-Tris Plus gels, and can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt® Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies being used. Each Bolt® Mini Gel Tank includes: • Gel Runner Tank • Bolt® Tank Base • Bolt® Tank Lid • Cassette Clamps, Right and Left • Novex® Power Supply Adapters • Gel Knife About Bolt® Bis-Tris Pl...
Catalog: B4477599
Size: 1 unit

MAX Efficiency® DH10Bac™ Competent Cells

Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells

MAX Efficiency® DH10Bac™ Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac® Baculovirus Expression System . The DH10Bac™ E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac™, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency® DH10Bac™ Competent Cells include: • Consistent yields of >1 × 10 8 transformants/ µg • Tolerance of small amounts of undiluted ligation reactions • Support of efficient blue/white colony screening (Φ80 lac ZΔM15 marker) About MAX Ef...
Catalog: 10361-012
Size: 5 × 100 µl

pCMV/Bsd Vector

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

Blasticidin is a potent selection agent that allows selection of stable mammalian cell lines in as little as one week. The BsdCassette™ vectors simplify the construction of custom vectors that use Blasticidin resistance as a selectable marker. Each BsdCassette™ vector is designed for use in a specific host system and offers: A strong host promoter to drive expression of the Blasticidin resistance gene ( bsd )(Table 1) Two large polylinkers flanking the promoter and bsd gene to facilitate the construction of Blasticidin resistant vectors Each BsdCassette™ vector carries the synthetic EM-7 promo...
Catalog: V510-20
Size: 20 µg

CDK8⁄Cyclin C Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

CDK8 phosphorylates the carboxy-terminal domain of the largest subunit of RNA polymerase II. This protein is coexpressed and copurified with CyclinC.
Catalog: PV4402
Size: 10 µg

Alexa Fluor® 488 Phalloidin

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Selectively stains F-actin • Outstanding fluorescence performance • Excitation/Emission: 495/518 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples Get Superior Results in Your Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Flu...
Catalog: A12379
Size: 300 units

6-FAM™ Dye Phosphoramidite, 45 mg

Category: Products & Services > Buffers & Chemicals > General Chemicals > Oligo Synthesis Reagents

Contains 45 mg 6-FAM™ Dye Phosphoramidite. 6-FAM™ Dye Phosphoramidite is used for the 5’ attachment of FAM™ dye during oligonucleotide synthesis. 6-FAM™ Dye Phosphoramidite is an optically pure isomer (not a mixture). The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesi...
Catalog: 403169
Size: 45 mg

MitoTracker® Red FM - Special Packaging

Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains

MitoTracker® Red FM is a far red-fluorescent dye (abs/em ~581/644 nm) that stains mitochondria in live cells and its accumulation is dependent upon membrane potential.
Catalog: M22425
Size: 20 × 50 µg

One Shot® BL21(DE3) Chemically Competent E. coli

Category: Products & Services > Competent Cells & Strains > Expression Competent Cells

BL21(DE3) E. coli are ideal for use with bacteriophage T7 promoter-based expression systems ( e.g. , pRSET, pCR¤T7, and pET). BL21(DE3) carry the lambda DE3 lysogen. Recombinant proteins that are non-toxic to E. coli are generally expressed at higher levels in BL21(DE3) cells than in BL21(DE3)pLysS or BL21(DE3)pLysE. However, the basal expression levels of heterologous genes are significantly higher in BL21(DE3) than in BL21(DE3)pLysS or BL21(DE3)pLysE.
Catalog: C6000-03
Size: 20 × 50 µl

Invitrolon™ PVDF/Filter Paper Sandwich, 0.45-µm pore size, 8.5 cm x 13.5 cm

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Blotting Membranes > PVDF Membranes

Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
Catalog: LC2007
Size: 16⁄pack

EnzChek® Lysozyme Assay Kit - 400 Assays

Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Other Enzyme Assay Kits

The EnzChek® Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme acitivty in solution. The assay measures lysozyme activity on Micrococcus lysodeikticus cell walls, which are labeled to such a degree with fluorescein that the fluorescence is quenched. Lysozyme action can relieve this quenching, yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.
Catalog: E22013
Size: 1 kit

Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L), highly cross-adsorbed

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

This highly cross-adsorbed Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L) is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG and human serum.
Catalog: A-11029
Size: 0.5 ml

Alexa Fluor® 488 Donkey Anti-Goat IgG (H+L)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

The Alexa Fluor® 488 Donkey Anti-Goat IgG (H+L) is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from goat. To minimize cross-reactivity, it has been adsorbed against rabbit, rat, mouse, and human IgG.
Catalog: A-11055
Size: 0.5 ml

Fura-FF, Pentapotassium Salt, cell impermeant

Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores

Fura-FF pentapotassium salt is a fura-2 derivative that has Ca 2+ sensitivity similar to mag-fura-2, but is relatively insensitive to Mg 2+ . This water-soluble salt form is useful for intracellular loading by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: F14180
Size: 500 µg

NuPAGE® Novex® 3-8% Tris-Acetate Gel 1.0 mm, 2D Well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > 2-D Gels

NuPAGE® Novex Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels...
Catalog: EA0376BOX
Size: 1 box

NucBlue® Fixed Cell ReadyProbes™ Reagent

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes™ Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Fixed Cell ReadyProbes™ Reagent: Ready-to-use liquid DAPI formulation No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or...
Catalog: R37606
Size: 1 kit

TaqMan® Universal Master Mix II, no UNG Mini-Pack (1 x 1 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

TaqMan® Universal Master Mix II is a convenient 2X mix for use in all real-time application including microRNA, gene expression and genotyping. TaqMan® Universal Master Mix II is a convenient 2X mix that includes: -AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure), a highly purified thermostable DNA polymerase, that is hot start-enabled for convenient reaction setup at room temperature -Available in two formats: with and without uracil-DNA glycosylase (UDG) and dUTP⁄dTTP mix to minimize carryover PCR contamination -Optimized salt, dNTP, and buffer concentrations for reliable performance -Passive ...
Catalog: 4440043
Size: 40 reactions

GeneAmp® 10X PCR Gold Buffer & MgCl2, 6 Sets (6 x 1.5 mL)

Category: Products & Services > Buffers & Chemicals > Molecular Biology Enzyme Buffers > PCR Enzyme Buffers

Applied Biosystems® GeneAmp®10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold® DNA Polymerase, resulting in highly specific, robust PCR amplification. When diluted 1:10, provides the optimal pH and ionic strength for PCR activation and amplification with AmpliTaq Gold® DNA Polymerase, ensuring maximum performance Available in several convenient sizes GeneAmp® 10X PCR Buffer II is both magnesium ion free and gelatin free. A separate MgCl 2 solution is provided. When used together, GeneAmp® 10X PCR Buffer II and MgCl 2 solution offer more versatility in...
Catalog: 4306898
Size: 6 × 1.5 ml

Streptavidin-Peroxidase

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin

This enzyme conjugate solution can be used to stain biotinylated antibodies.
Catalog: 50-209Z
Size: 15 ml

MEM, HEPES, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • HEPES • L-glutamine • Phenol Red
Catalog: 12360-038
Size: 500 ml

Geneticin® Selective Antibiotic (G418 Sulfate), 50 mg/ml Solution

Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics

Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a 50 mg / ml solution in...
Catalog: 10131-019
Size: 20 ml

ViiA™ 7 System TaqMan® Array Upgrade Kit

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Real-Time PCR Instrument Accessories

This kit allows the ViiA™ 7 Real-Time PCR System to operate using a TaqMan® Array Block. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4453546
Size: 1 upgrade

Covaris® M220 Focused-ultrasonicator™

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing

The Covaris® M220 Focused-ultrasonicator™ is designed for next generation sequencing applications that require high-quality DNA fragmentation for library preparation. The Covaris® M220 efficiently focuses the minimum amount of energy required directly into a sample, enabling precise control of the hydrodynamic shearing forces that fragment nucleic acids to the selected size. The process is isothermal, so fragmentation is unbiased, the sample is undamaged, and yields are high. The Covaris® focused-ultrasonic technology is considered a DNA shearing standard in many labs worldwide. The Covaris® M...
Catalog: 4482277
Size: 1 instrument

Custom TaqMan® SNP Genotyping Assay, Non-Human LG

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

Custom TaqMan® SNP Genotyping Assay, Non-Human LG
Catalog: 4332076
Size: 750 µl

Gateway® pT-Rex™-DEST30 Vector

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12301-016
Size: 6 µg

TA Cloning® Kit, Dual Promoter, with pCR™II Vector, without Competent Cells

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > PCR Cloning Vectors

The TA Cloning® Kit Dual Promoter with pCR™II vector provides a quick, one-step cloning strategy for directly inserting a Taq -amplified PCR product into a plasmid vector. The T7 and Sp6 promoters of the pCR™II vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCR™II cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts. Features of the TA Cloning® Kit Dual Promoter: • Fast &...
Catalog: K2070-40
Size: 40 reactions

Serine Pyruvate Aminotransferase (AGXT) Monoclonal Antibody

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

This mouse monoclonal antibody recognizes human, rat, and bovine serine pyruvate aminotransferase (AGXT). Antibody Specifications Applications: Immunoprecipitation, Immunocytochemistry, In-Cell ELISA Species Cross-Reactivity: Human, Rat, Bovine Clone ID: 1F9BC7 Host Species and Isotype: Mouse IgG1, κ
Catalog: A21964
Size: 100 µg

SKP2 (S-Phase Kinase-Associated Protein 2) Mouse Monoclonal Antibody (clone 2C8D9), Concentrate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

SKP2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. In tumor tissues, SKP2 is expressed widely, such as colon mucinous carcinoma, gastric adenocarcinoma, breast cancer, melanoma, prostate adenocarcinoma, with particularly higher expression on lung adenocarcinoma, lung squamous carcinoma, squamous carcinoma of cervix, endometrial carcinoma, ovary serous carcinoma and leiomyosarcoma. A recent study suggests that SKP2 plays a role in oncogenesis and is involved in the pathogenesis of lymphomas. Positive tissue: Breast ca...
Catalog: 18-7334
Size: 0.5 ml

CD73 Monoclonal Antibody, Mouse (7G2)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

• Reactivity -Human • Applications -Flow Cytometry, Functional Studies, Immunohistochemistry (Frozen)*, Immunoprecipitation*, Inhibition* • Host, Isotype -Mouse, IgG2a-κ • Clonality, Clone -mAb, 7G2 *Application tested by outside source. Ms anti-CD73; Unconjugated Monoclonal antibody specific to Human CD73 . This antibody is validated for use in Flow Cytometry, Functional Studies, Inhibition of E-cadherin-dependent cell-to-cell contact, Immunohistochemistry, Immunoprecipitation. Anti-CD73 recognizes the expressed product of the NT5E gene.
Catalog: 41-0200
Size: 100 µg

BGH Reverse Primer

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Sequencing Primers

It is a sequencing primer to sequence mammalian expression vectors having the BGH polyadenylation signal. Primer is supplied as 2 µg which equals 358 pMoles. The melting temperature is 54.8°C. The sequence of the primer is: 5'd[TAGAAGGCACAGTCGAGG]3'
Catalog: N575-02
Size: 2 µg

TaqMan® Array Mouse Immune Response

Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays

The Applied Biosystems® TaqMan® Array Mouse Immune Response 96-well Plate contains 92 assays to immune response associated genes and 4 assays to candidate endogenous control genes. The panel of assays in this plate targets genes from immune system functions that fall into 9 classes: Cell Surface Receptors; Stress Response; Oxidoreductases; Proteases; Transcription Factors; Signal Transduction; Cytokines and Cytokine Receptors; Chemokines and Chemokine Receptors; and Cell Cycle and Protein Kinases. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan...
Catalog: 4414079
Size: 1 standard plate

Alexa Fluor® 647 C 2 Maleimide

Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels

Conjugates prepared with the thiol-reactive Alexa Fluor 647® C 2 maleimide are spectrally similar to the Cy5 dye, resulting in an optimal match to optical filters for the Cy5 dye. Total fluorescence of secondary antibody conjugates of Alexa Fluor® 647 dye, however, is significantly higher than that of Cy5 conjugates. Unlike the Cy5 dye, Alexa Fluor® 647 dye has very little change in absorption of fluorescence spectra when conjugated to most porteins, oligonucleotides, or nucleic acids, yielding greater total fluorescence at the same degree of substitution.
Catalog: A-20347
Size: 1 mg

HiMark™ Unstained Protein Standard

Category: Products & Services > Controls, Standards & Ladders > Protein Standards & Ladders > Unstained Protein Standards

The HiMark™ Unstained High Molecular Weight (HMW) Protein Standard is designed for analysis of high molecular weight proteins on Novex® NuPAGE® Tris-Acetate Gels . • Comprehensive – Contains markers with a wide range of high molecular weights, providing 9 protein bands in the range of 30-460 kDa • Convenient – A ready-to-use formulation eliminates the need to heat, reduce, or add sample buffer prior to use • Compatible – Intended for use with NuPAGE® Novex 3-8% and 7% Tris-Acetate Gels under denaturing conditions* *Note: HiMark™ Unstained High Molecular Weight Protein Standard can also be used...
Catalog: LC5688
Size: 250 µl

NativePAGE™ Running Buffer Kit

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers

The NativePAGE™ Running Buffer Kit contains the NativePAGE™ Running Buffer (20X), which is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels. The kit also contains the NativePAGE™ Cathode Buffer Additive (20X) that is used with the NativePAGE™ Running Buffer to make the Cathode Running Buffer for the system.
Catalog: BN2007
Size: 1 kit

Alexa Fluor 350 Donkey Anti-Mouse IgG

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

The Alexa Fluor 350 donkey anti - mouse IgG is labeled with our bright, photostable, blue-fluorescent Alexa Fluor 350 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. The donkey anti - mouse IgG antibodies show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins.
Catalog: A10035
Size: 0.5 ml

Purified Rat IgG

Category: Products & Services > Antibodies & Secondary Detection Reagents > Isotype Antibody Controls

Catalog: 02-9602
Size: 5 mg

Gibco® Astrocyte Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Neuronal Cell Media

GIBCO® Astrocyte Medium has been specially formulated to support the growth of primary human and rat astrocytes while retaining their phenotypes. The medium has been internally validated and optimized to provide an optimal balance between cell growth and the maintenance of cell viability, normal phenotype, and function. If more rapid proliferation is desired, addition of an appropriate amount of epidermal growth factor (EGF) is recommended, although cells may exhibit morphological and phenotypic changes. Astrocytes are a type of glial cells of the nervous system with a typical star shape. It h...
Catalog: A12613-01
Size: 500 ml

Precision Plate Holder for 7300 & 7500 Systems

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Adhesive Film & Other Plate-Related Accessories

This precision plate holder is for use with Applied Biosystems® 7300 real-time PCR systems (SN 273000878 and above), as well as 7500 real-time PCR systems (SN 275001330 and above). Customers with earlier serial numbers should contact Product Support .
Catalog: 4367032
Size: 1 each

TaqMan® GTXpress™ Master Mix, Mini-Pack (1 mL)

Category: Products & Services > Kits & Assays > Genotyping Kits > SNP Genotyping Kits

The TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction). • Compatible with both Fast and Standard mode thermal cycling conditions • Pre-mixed components stored at 2°–8°C significantly reduce assay setup time • Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays •...
Catalog: 4403311
Size: 400 reactions

CaptAvidin™ Biotin-Binding Protein

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Avidin

CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a K a of ~10 9 M -1 . This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.
Catalog: C-21385
Size: 1 mg