Products from T7 Promoter Primer to Nupage® Transfer Buffer (20x) (1 L)
Invitrogen offers primers for PCR amplification that complement many of the vectors currently available. All primers are: Desalted and purified by gel filtration Assayed for function by PCR amplification Provided in 2 µg quantity T7: 5´- TAATACGACTCACTATAGGG- 3´
Catalog: N560-02
Size: 2 µg
Size: 2 µg
Goat whole molecule anti-human immunoglobulin Format/Formulation: HRP Conjugate
Catalog: AHI0604
Size: 0.7 mg⁄ml
Size: 0.7 mg⁄ml
The Applied Biosystems® MicroAmp® Caps Strips are designed to fit on MicroAmp® Reaction Tubes, reaction strips and 96-well plates. They are available in 8-cap and 12-cap strips in clear and colored sets of red, orange, blue, and green. MicroAmp® Optical 8-Cap Strip should be used for Real-Time PCR applications. Attach caps using the MicroAmp® Cap Installing Tool. • Will not interfere with sample reading • Provides tight seal to minimize evaporation • Easy to apply and remove Minimize Evaporation Use MicroAmp® Caps Strips to seal the tubes containing your precious samples. This will reduce the ...
Catalog: N8010535
Size: 300 strips
Size: 300 strips
GeneScan™ 500 TAMRA™ Size Standard is an internal lane size standard developed for use with the Applied Biosystems fluorescence- based DNA electrophoresis systems. The use of an internal lane size standard during electrophoresis enables automated data analysis and precise DNA fragment size comparisons between electrophoresis runs. GeneScan™ 500 TAMRA™ Size Standard is designed for sizing DNA fragments in the 35-500 bp range and provides 16 single-stranded labeled fragments of 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490, and 500 bases. Each of the DNA fragments is lab...
Catalog: 401733
Size: 800 reactions
Size: 800 reactions
STEM PRO-34
Catalog: 10640019
Size: 1 each
Size: 1 each
TaqMan® MGB Probe
Catalog: 4316034
Size: 6,000 pmol
Size: 6,000 pmol
Applied Biosystem® AmpErase® Uracil N-glycosylase (UNG), part of the GeneAmp® PCR Carry-over Prevention Kit, is a 26 kDa ultrapure, recombinant enzyme encoded by the E. coli uracil N-glycosylase gene, which has been inserted into an E. coli host to direct the expression of the wild type form of the enzyme. The GeneAmp® PCR Carry-over Prevention Kit provides reagents for a simple yet powerful method of ensuring that PCR products cannot be reamplified in subsequent PCR amplifications, thereby preventing false positive results. • Enzymatic method stops PCR carryover contamination, which prevents ...
Catalog: N8080096
Size: 100 units
Size: 100 units
Normal goat serum. Whole serum is obtained from non-immunized (preimmune) healthy goats. The serum is clarified through a 0.8 micron low protein binding filter.
Catalog: PCN5000
Size: 5 ml
Size: 5 ml
The 1X TE Buffer is a component in the PureLink™ 96 Plasmid Purification System. The 1X TE buffer is used to resuspend the final purified plasmid pellet and contains very low EDTA, so it is compatible with sequencing and other enzymatic applications.
Catalog: 12090-015
Size: 100 ml
Size: 100 ml
All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V855-20
Size: 20 µg
Size: 20 µg
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryoti...
Catalog: A10462
Size: 10 µg
Size: 10 µg
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression • att R sites for Gateway® cloning, enabling recombination with att L-flanked fragments •C-terminal V5 tag for easy detection •Neomycin resistance gene for stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli Gateway® Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination ve...
Catalog: 12489-019
Size: 6 µg
Size: 6 µg
This pcDNA™3.1/Hygro (-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Hygromycin selectable marker and a reverse-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+...
Catalog: V875-20
Size: 20 µg
Size: 20 µg
NuPAGE® LDS Sample Buffer (4X) (10 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample...
Catalog: NP0007
Size: 10 ml
Size: 10 ml
Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion® RNase V1, RNA-Grade cleaves base-paired nucleotides and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites. Supplied in one tube of 200 µl (0.1 U/ µL); optimization reagents also included. In addition to applications for RNA structural analysis, RNA-Grade ribonucleases can be used to map protein binding sites on RNAs by comparing cleavage patterns in the presence and absence of an RNA binding p...
Catalog: AM2275
Size: 200 µl
Size: 200 µl
Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR product is directionally cloned with efficiencies typically >99%. The pDONR™221 vector haa a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
Catalog: 12536-017
Size: 6 µg
Size: 6 µg
Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco® Versene Solution (0.48 mM) is formulated as 0.2 g EDTA(Na 4 ) per liter of Phosphate Buffered Saline (PBS). Product Use For Research Use Only. Not for use in diagnostic procedures. Dual-site cGMP Manufacturing and Quality System Gibco® Versene Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technolog...
Catalog: 15040-066
Size: 100 ml
Size: 100 ml
The One Shot® Stbl3™ E. coli strain is designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli , these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower™ Lentiviral Expression Vector and other retroviral vectors. The DNA yield from Stbl3™ cells is often >10-fold higher than alternative strains such as TOP10 (Table 1). One Shot® Stbl3™ Chemically Competent Cells deliver: • Optimal performance for the propagation of lentiviral vectors • Enhanced genomic cloning capacity ( mcr B, mrr ) • Ex...
Catalog: C7373-03
Size: 20 × 50 µl
Size: 20 × 50 µl
pcDNA™5/TO is a 5.7 kb expression vector designed for use with the T-REx™ System (Catalog nos. K1020-01 and K1020-02) available from Invitrogen. The vector allows tetracycline-regulated expression of the gene of interest in mammalian host cells expressing the Tet repressor (TetR) from the pcDNA™6/TR vector (Catalog no. V1025-20). Details about the pcDNA™5/TO vector can be obtained from the manual that can be downloaded from our website with V1025-20.
Catalog: V1033-20
Size: 20 µg
Size: 20 µg
The ARCTURUS® PicoPure® RNA Isolation Kit is designed to recover high-quality total RNA consistently from fewer than ten cells, even from a single cell. This kit is designed to provide consistent, efficient RNA recovery without sacrificing quality. Small volume elution allows you to maximize your recovery of RNA from small numbers of cells for use with your gene expression analysis. Key Product Features: • Greater sensitivty—isolate RNA from even a single cell • Superior efficiency—maximize RNA recovery from samples • Streamlined concentration—elute with small volumes • Flexible application—co...
Catalog: KIT0204
Size: 40 samples
Size: 40 samples
Applied Biosystems® Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N) 6 ] that anneal to random complementary sites on a target DNA or RNA, to serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase. With radiolabeled nucleotides present, the DNA is labeled quickly and reliably to high specific activity. Note : See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostics procedures.
Catalog: N8080127
Size: 1 kit
Size: 1 kit
Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 25 reactions. • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time • Amplifies aRNA for gene array analysis and other applications • Efficiently incorporates many modified nucleotides A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® k...
Catalog: AM1333M
Size: 25 rxns w⁄ Manual
Size: 25 rxns w⁄ Manual
Goat whole molecule anti-human immunoglobulin Format/Formulation: HRP Conjugate
Catalog: AHI0704
Size: 0.7 mg⁄ml
Size: 0.7 mg⁄ml
Applied Biosystems® TaqMan® Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® real-time PCR instru...
Catalog: 4304437
Size: 200 reactions
Size: 200 reactions
1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels. Round, easy to remember ba...
Catalog: 10787-018
Size: 250 µg
Size: 250 µg
The protein concentration of each lot of Bovine Pituitary Extract is determined by the Bradford method. Each vial of BPE contains not less than 25 mg of protein.
Catalog: 13028-014
Size: 25 mg
Size: 25 mg
PI4KB and PI4KA are the two human type III PI4 kinases, which share sequence similarly with PI3 kinases and phosphorylate the 4 position of phosphotidyl inositol.
Catalog: PV5277
Size: 20 µg
Size: 20 µg
NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting.
Catalog: FNN0021
Size: 100 ml
Size: 100 ml
SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A) + RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity...
Catalog: 18080-044
Size: 10,000 units
Size: 10,000 units
The pFastBac™ Dual vector features two promoters in a single vector for expression of two proteins simultaneously in insect cells when using the Bac-to-Bac® Baculovirus Expression System. The vector has two strong promoters, the polyhedrin promoter and the p10 promoter, for high-level expression. An expression control is included that expresses both CAT and β-glucuronidase (gus).
Catalog: 10712-024
Size: 10 µg
Size: 10 µg
Collagen I, Rat Tail is the cell culture workhorse of basic coating and gel preparations for growing attachment cells. Gibco® Collagen I, Rat Tail is now available in pre-coated tissue culture plates to help save time at the bench. Designed to meet a number of different applications, our high quality, consistent Collagen I Coated Plates come in 3 formats – 96 well, 24 well and 6 well plates . • Ready to use, room temperature storage • Quality tested to help ensure consistency • Available in 3 different plate formats and in custom formats for a broad range of applications Easy to use, easy to s...
Catalog: A11428-02
Size: 5 plates
Size: 5 plates
One Shot® BL21-AI™ E. coli are chemically competent cells designed for applications that require tight regulation and strong expression of toxic proteins from any T7 promoter-based expression systems. One Shot® BL21-AI™ Chemically Competent cells have a transformation efficiency of >1×10 8 cfu/ µg plasmid DNA. • Ideal for inducing expression of toxic protein • Gene expression is regulated by the addition of L-arabinose to culture • Suitable for use with any T7 promoter-based plasmids • Supplied in a convenient One Shot® format Excellent Expression of Toxic Recombinant Proteins One Shot® BL21-A...
Catalog: C6070-03
Size: 20 × 50 µl
Size: 20 × 50 µl
Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907
Size: 50 reactions
Size: 50 reactions
Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR®-Blunt II-TOPO® Vector also features: • ccdB gene for positive selection (2) • Eco R I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for...
Catalog: K2800-02
Size: 20 preps
Size: 20 preps
Custom Media: 2-MERCAPTOETHANOL
Catalog: A13558SA
Size: 1 each
Size: 1 each
Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions. Invitrogen offers a wide selection of single-strand primers that may be used in either single- or double-stranded sequencing protocols. All sequencing primers are non-phosphorylated and are supplied lyophilized. All primers are: Desalted and purified by gel filtration Assayed for function in automated sequencing reactions Supplied in quantity of 2 µg
Catalog: N520-02
Size: 2 µg
Size: 2 µg
The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination. • Enables individual oligonucleotide design for real-time PCR applications. • Supports assays based o...
Catalog: 4363991
Size: 1 license
Size: 1 license
All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V810-20
Size: 20 µg
Size: 20 µg
Our Magnetic Particle Concentrators (MPC®) enable optimal binding of Dynabeads® magnetic beads with their targets (e.g., cells, proteins, and nucleic acids) in a variety of starting samples. The MPC®-S was designed specifically for microbiology applications; it has been validated with select Dynabeads® for enrichment of E. coli O157:H7, Salmonella, non-O157 STEC, Cryptosporidium, and Giardia. The MPC®-S can be used with both food enrichment samples types as well as water samples. It holds up to six microcentrifuge tubes and has an optimal working volume range of 20 µl–2 ml. Note that this magn...
Catalog: A13346
Size: 1 unit
Size: 1 unit
One Shot® ccd B Survival™ 2 T1 Phage-Resistant (T1 R ) chemically competent E. coli are suitable for the propagation of plasmids containing the ccd B gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and super-coiled entry vectors. Transformation efficiency is >1 × 10 9 transformants/ µg DNA. The ccd B Survival™ 2 T1 R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1 R E. coli strain offers the following Features: • Resistance to the ccd B gene product • Resistance to T1 and T5 phage (the ton A/ fh...
Catalog: A10460
Size: 10 transformations
Size: 10 transformations
Use Applied Biosystems® MicroAmp® Adhesive Film Applicator to form a tight seal between a microplate and an adhesive film. Form a Tight Seal The MicroAmp® Adhesive Film Applicator enables you to ergonomically push an adhesive film down upon a microplate to form a tight seal and minimize air bubbles. This tight seal will prevent well-to-well contamination and sample loss. Quick and Easy Use the MicroAmp® Adhesive Film Applicator to speed the process of sealing multiple microplates. This tool is designed according to the microplate dimensions so you can quickly seal a microplate. The rugged prop...
Catalog: 4333183
Size: 5 applicators
Size: 5 applicators
Replacement Parts for Electrophoresis Apparatus
Catalog: EI9052
Size: each
Size: each
Alexa Fluor 488 rabbit anti—rat IgG is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rat. To minimize crossreactivity, it has been adsorbed against human IgG prior to conjugation.
Catalog: A-21210
Size: 0.5 ml
Size: 0.5 ml
UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and Quality Testing: No DNase, RNase, or protease activity detected
Catalog: 15568-025
Size: 1 L
Size: 1 L
NATIVEPAGE 3-12% GELS, 10-WELL
Catalog: BN2011BX10
Size: 1 each
Size: 1 each
The IFN-β Mouse ELISA Kit quantifies natural and recombinant mouse IFN-β in serum and cell culture supernatant. Interferon-β (IFN-β) is a cytokine that is produced by the cells of the immune system.
Catalog: KMC4041
Size: 96 tests
Size: 96 tests
Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR • The optimized formulation contains highly purified AmpliTaq Gold® D...
Catalog: 4367659
Size: 200 reactions
Size: 200 reactions
The Ion Library Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. This comprehensive kit provides a TaqMan® qPCR mix and library standard to detect and quantify femtomolar amounts of Ion Fragment Libraries. With universal cycling conditions that allow both fast and standard cycling, the Ion Library Quantitation kit supports most real-time platforms. This universal kit provides greater accuracy and specificity compared to non-probe-based methods, delivering simplicity and speed to the PGM™ ...
Catalog: 4468802
Size: 250 reactions
Size: 250 reactions
The Bioruptor® UCD-200 is a benchtop sonication device used to uniformly process up to 12 x 0.5 ml samples simultaneously in sealed tubes without aerosol formation. Consisting of a high-power ultrasound generating element located below a water bath, the Bioruptor® sonicates at a 20 kHz frequency, similar to a probe sonicator. The Bioruptor® comes with standard 0.5 ml accessories and the BioBox-200 for soundproofing. The Bioruptor® UCD-200 Provides: • Parallel processing of up to 12 DNA samples • Robust and reproducible results • Automated sample prep Get Reproducible DNA Shearing Easily and Au...
Catalog: 4465622
Size: 1 each
Size: 1 each
NuPAGE® Transfer Buffer (20X) (1 L) is a buffer used to transfer proteins from NuPAGE® gels to membranes for western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use. Related Link • Learn more about other Invitrogen products for your protein transfer needs .
Catalog: NP0006-1
Size: 1 L
Size: 1 L
Products from T7 Promoter Primer to Nupage® Transfer Buffer (20x) (1 L)
View products by:
Title |
Brand |
Price |
Popularity |
Best Sellers
Popular items by:
Popularity |
Our Choices |
All-Round Favourites |
Title
