Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Buffer: PBS Preservative: 0.1% Sodium Azide
Catalog: 10000C
Size: 25 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum > Serum From Other Blood
Sera Category: Specialty Origin: New Zealand.
Catalog: 16210-064
Size: 100 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
GPX1 rabbit monoclonal antibody recognizes human glutathione peroxidase 1 (GPX1) in western blot and immunoprecipitation applications. Antibody Specifications Applications: Western blot, immunoprecipitation Species Reactivity: Human Clone ID: C8C4 Host Species and Isotype: Rabbit IgG
Catalog: A14011
Size: 100 µl
Category: Products & Services > Eukaryotic Cells > Cell Lines > Mammalian Cell Lines
The GripTite™ 293 MSR Cell Line is a genetically engineered Human Embryonic Kidney (HEK 293) cell line that expresses the human macrophage scavenger receptor and strongly adheres to standard tissue culture plates for dependable results. Developed from a 293-H subclone, GripTite™ 293 MSR cells show the same fast cell growth, high transfection efficiency, and high-level expression characteristics of the parental 293-H cells. Unlike most 293 cells, GripTite® 293 MSR cells dont wash away during the repeated manipulations of routine tissue culture or plate washing protocols (Figure 1). Their superi...
Catalog: R795-07
Size: 1 kit
Category: Products & Services > Buffers & Chemicals > Denaturing Agents
UltraPure™ Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations. It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas (1).
Catalog: 15535-016
Size: 500 g
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides
BODIPY FL adenosine 5'-triphosphate (A-12410) and BODIPY FL guanosine 5'-triphosphate are composed of a BODIPY FL fluorophore attached to the 2' or 3' position of the ribose ring via a linker. Interactions between the fluorophore and the purine base are evident from the spectroscopic properties of these nucleotide analogs. The fluorescence quantum yield of BODIPY FL GTP is only about one-tenth that of BODIPY FL ATP; however, this yield may be enhanced upon binding of BODIPY FL GTP to GTP-binding proteins.
Catalog: G-12411
Size: 100 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Unconjugated polyclonal antibody specific to human H3K27me2. This antibody is validated for use in Chromatin Immunoprecipitation (ChIP), ELISA, dot blotting and Western blotting. This antibody has been raised against the region of the histone H3 containing the dimethylated lysine 27 (H3K27me2), using a KLH-conjugated synthetic peptide. Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histo...
Catalog: 49-1013
Size: 50 µg
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > F-12 Nutrient Mixture
Ham's F-12K (Kaighn's) medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) medium was developed for primary human hepatocytes, as well as some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) medium is manufactured as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 21127-030
Size: 10 × 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > F-12 Nutrient Mixture
Ham's F-12K (Kaighn's) medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) medium was developed for primary human hepatocytes, as well as some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) medium is manufactured as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 21127-022
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > HAT & HT Supplements
Gibco® HAT Supplement is a liquid mixture of sodium hypoxanthine (5 mM), aminopterin (20 µM) and thymidine (0.8 mM). HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT–myeloma cells. Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT- contributed by the fused spleen cell. Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway. Product Use For Research Use Only. Not for use in diagnostic procedures. Dual site cGMP Manufac...
Catalog: 21060-017
Size: 100 ml
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
HCS CellMask™ Red stain (H32712) replaces the HCS CellMask™ Red cytoplasmic⁄nuclear stain (H32711) as a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask™ stains can be used for measuring DNA content in ...
Catalog: H32712
Size: 1 set
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Green neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid s...
Catalog: H34475
Size: 1 each
Category: Products & Services > Kits & Assays > Cell Analysis Kits > HCS Detection Kits
The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid sta...
Catalog: H34476
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Blocks
• The Heat Equalizing Block is an aluminum conduction plate for use with the SSP UniTray and other laboratory procedures that require even heat distribution during thermal cycling. The Heat Equalizing Block is set on top of the SSP UniTray while thermal cycling to ensure even heat distribution • The Membrane Comb Loading Tray is for convenient benchtop loading of 64-lane membrane combs. Polycarbonate tray with two rows of 64 wells, each 2 mm wide • HLA Beyond Tears - By Glenn E. Rodey, Second Edition (De Novo, Inc., Durango, CO 2000) Serves as an introductory text for individuals who are inter...
Catalog: 90000-1D
Size: 1 each
Category: Products & Services > Buffers & Chemicals > General Buffers > HEPES Buffer
Gibco® HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO 2 incubator. HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance. Dual-site cGMP Manufacturing and Quality System Gibco® HE...
Catalog: 15630-130
Size: 20 × 100 ml
Category: Products & Services > Buffers & Chemicals > Denaturing Agents
The highly deionized (Hi-Di) Formamide is used to resuspend samples before electrokinetic injection in capillary electrophoresis systems. The smaller configuration (5 ml) minimizes the number of freeze⁄thaw cycles compared to the larger 25ml bottle and may be a better fit when running a lower number of samples. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4440753
Size: 4 × 5 ml
Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits
The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 2 tubes of RNase inhibitor (100 µL each). The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is i...
Catalog: 4374967
Size: 1000 reactions
Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits
The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of this kit: • Fast —short reaction time (typically 30–60 min) • Convenient —easy workflow with few pipetting steps (2 tubes) • Reliable —reverse transcription of both abundant and limited targets • Flexible —optimized 2-step protocol, enabling multiple PCR reactions from a single reverse transcription reaction Streamline your cDNA synthesis while maintaining detection sensitivit...
Catalog: 4387406
Size: 50 reactions
Category: Products & Services > Controls, Standards & Ladders > Protein Standards & Ladders > Unstained Protein Standards
The HiMark™ Unstained High Molecular Weight (HMW) Protein Standard is designed for analysis of high molecular weight proteins on Novex® NuPAGE® Tris-Acetate Gels . • Comprehensive – Contains markers with a wide range of high molecular weights, providing 9 protein bands in the range of 30-460 kDa • Convenient – A ready-to-use formulation eliminates the need to heat, reduce, or add sample buffer prior to use • Compatible – Intended for use with NuPAGE® Novex 3-8% and 7% Tris-Acetate Gels under denaturing conditions* *Note: HiMark™ Unstained High Molecular Weight Protein Standard can also be used...
Catalog: LC5688
Size: 250 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Epitope Tag & Reporter Specific Antibodies
This purified Mouse Histidine (C-term) Tag Monoclonal Antibody recognizes a polyhistidine amino acid sequence at the carboxy-terminus of proteins. The free carboxy-terminus of the terminal histidine residue is a key element of the epitope recognition site of the anti-His (C-term) Antibody. The Anti-His(C-term) Monoclonal Antibody recognizes the sequence: -His-His-His-His-His-His-COOH . Validated applications for Histidine (C-term) Tag monoclonal antibody are western blotting and ELISA. Antibody Specifications: • Applications: Validated applications for Histidine (C-term) Tag Monoclonal Antibod...
Catalog: R931-25
Size: 50 µl
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Counting & Flow Cytometer Accessories
This is a cold block for use with the ARCTURUS® HistoGene® LCM Immunofluorescence Staining Kit. For Research Use Only. Not for use in diagnostics procedures.
Catalog: HIS0101
Size: 1 each
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Laser Capture Microdissection (LCM) Kits
The ARCTURUS® HistoGene® LCM Immunofluorescence Staining Kit is designed to retrieve high-quality nucleic acids from immunofluorescently-stained, frozen tissue using a quick 15-minute process. The kit provides reagents and slides for convenient and reliable immunoflourescent staining as well as protocols that are streamlined and optimized to maintain nucleic acid quality. Key product features are: • Simplified staining—retrieve high-quality RNA from simplified fluorescent target cell labeling • Efficient preservation—quick 15-minute process preserves mRNA • Cold sample protection—chill samples...
Catalog: KIT0420
Size: 32 applications
Category: Products & Services > Buffers & Chemicals > Microscopy Reagents
This mounting solution is designed for permanent mounting of DAB and BCIP/NBT. Dehydrate tissue before use. This is the same solution that is provided in the Histostain® -SP DAB Kit.
Catalog: 00-8030
Size: 15 ml
Category: Products & Services > Kits & Assays > Cell Analysis Kits > IHC & ICC Kits
The HistoMouse-Plus kit is the 2nd generation HistoMouse kit for the detection of mouse primary antibodies on mouse tissue samples without causing background due to endogenous mouse Ig. Our patented BEAT™ technology (Blocking Endogenous Antibody Technology) is used to prevent the secondary antibody conjugate from reacting with endogenous mouse Ig. The LAB-SA detection method is used to amplify the signal. In addition, our HistoMouse-Plus kit includes the AEC Single Solution chromogen⁄ substrate system to create an intense red deposit around the antigen⁄antibody⁄enzyme complex in the sample. Re...
Catalog: 85-9541
Size: For 50 Slides
Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Kinase & Phosphatase Substrates
Matched and validated lipid and peptide substrates are available to complement the Adapta® Universal Kinase Assay. The assays developed with these reagents allow for primary or secondary screening of kinase inhibitors.
Catalog: PV5707
Size: 1 ml
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure Grade Hoechst 33342 is greater than or equal to 98% pure by HPLC.
Catalog: H21492
Size: 100 mg
Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Isolation Accessories
The Homogenizer is used with the Micro-to-Midi™ Total RNA Purification System to reduce the viscosity of difficult tissue samples between 5 and 30 mg in RNA isolation procedures.
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Human ACTB (beta actin) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_0...
Catalog: 4326315E
Size: 1000 reactions
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Other Serum-Free & Specialty Media
Human Endothelial-SFM is a basal serum-free growth medium developed to support the isolation and expansion of human umbilical venous and arterial endothelial cells (1–4). Medium requires supplementation with bFGF (20 ng/ml), EGF (10 ng/ml), and human plasma fibronectin (10 µg/ml). Contains L-glutamine.
Catalog: 11111-044
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Growth Supplements for Primary & Stem Cells
A sterile set of solutions intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. The HKGS Kit provides, in separate vials, all the components of complete HKGS (S-001-5). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional.
Catalog: S-001-K
Size: 1 kit
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > Purified Total RNA
The highest-quality RNA available from Ambion®, it is DNase-treated, subjected to unsurpassed quality control standards, and certified to contain small RNAs (miRNA, siRNA, and snRNA). One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment ...
Catalog: AM7960
Size: 100 µg
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Human PGK1 (phosphoglycerate kinase 1) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: PG...
Catalog: 4326318E
Size: 1000 reactions
Category: Products & Services > Eukaryotic Cells > Primary Cells > Hepatocytes
Cryopreserved human hepatocytes for use in transporter-mediated uptake applications. Cells per vial: 4-8 million Prototypical hepatic uptake transporter substrates taurocholate and estradiol-17β-glucuronide have been assessed to qualify the transport function of hepatic NTCP (SLC10A1) and OATP1B14/3 (SLCO1B1/3), respectively. The transport of each substrate (radiolabeled) was monitored by scintillation analysis and active transport values (37°C - 4°C) determined by linear regression. Get the Results You Need with GIBCO® Hepatocytes High quality hepatocytes, with high viabilities, in vivo -like...
Catalog: HMCPUS
Size: 1.5 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics
Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus . Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco® Hygromycin B is used as a bacterial selective antibiotic in the concentration range of 100 - 1000 µg / ml. This product is supplied as a 50 mg / ml solution. Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene ( hyg or hph ). Hygromycin B uses a different mode of action than Geneticin®, Blasticidin S, or Zeocin™, so it is excellent for use in dual-selec...
Catalog: 10687-010
Size: 20 ml
Category: Products & Services > Buffers & Chemicals > Immunoassay Buffers & Reagents > ELISA Buffers & Reagents
This Casein-based blocking reagent can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems. For Research Use Only. Not for use in diagnostics procedures.
Catalog: T2015
Size: 30 g
Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Blotting Systems
The iBlot® Gel Transfer Device performs Western blotting transfer efficiently and reliably, typically in 7 minutes or less without the need for liquid buffers. The iBlot® Gel Transfer Device is an integral part of the iBlot® Dry Blotting System, which consists of the transfer device and disposable blotting stacks which contain the buffers and the transfer membrane (nitrocellulose or PVDF). The iBlot® Dry Blotting System is for dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels, electroblotting of nucleic acids from agarose gels and TBE polyacrylamide gels, and performing Weste...
Catalog: IB1001
Size: 1 each
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Blotting Membranes > iBlot® Detection & Transfer Stacks
The iBlot® Transfer Stacks are used to transfer proteins using the iBlot® Gel Transfer Device. The iBlot® Transfer Stacks are disposable stacks that come in three varieties for different applications: iBlot® Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins, iBlot® DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA, and iBlot® Western Detection Stacks are used to perform Western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot® Transfer Stack contains a copper ...
Catalog: IB3010-32
Size: 3 x 10 sets⁄box
Category: Products & Services > Kits & Assays > ELISA Kits > ELISA
The IFN-β Mouse ELISA Kit quantifies natural and recombinant mouse IFN-β in serum and cell culture supernatant. Interferon-β (IFN-β) is a cytokine that is produced by the cells of the immune system.
Catalog: KMC4041
Size: 96 tests
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The IgG (γ), goat F(ab’), 2 R-phycoerythrin (R-PE) dye conjugate is a polyclonal goat antibody that is Fc specific and recognizes human IgG. R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence emission without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients. Use of R-PE in antibody conjugates results in probes with greatly enhanced detectability. Antibody Specifications Description: IgG (γ), Goat F(ab’)2 polyclonal, Fc specific Label: R-PE Validated Appl...
Catalog: H10104
Size: 0.5 ml
Category: Products & Services > Kits & Assays > ELISA Kits > ELISA
The IgG Subclass Human ELISA Kit profile contains components required to construct an enzyme-linked immunoassay for the specific and quantitative measurement of human IgG1, IgG2, IgG3, and IgG4 subclasses.
Catalog: 99-1000
Size: 192 tests
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Isotype: IgG1K Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Buffer: PBS Preservative: 0.1% Sodium Azide
Catalog: MH1722
Size: 0.5 ml
Category: Products & Services > Kits & Assays > ELISA Kits > ELISA
The IL-1RA ELISA Kit is designed to quantify protein levels of human interleukin-1 receptor antagonist (IL-1RA) in biological samples. IL-1RA is a cytokine which opposes the actions of IL-α and IL-1β by binding to IL-1 receptors.
Catalog: KAC1181
Size: 96 tests
Category: Products & Services > Kits & Assays > ELISA Kits > ELISA
The Mouse IL-6 ELISA research use only kit is designed to detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader. Easy to run Rapid protocol Convenient precoated, removable 8-well strips Easy-to-Run Sandwich ELISA The total assay incubation time is only 3.5 hours. A monoclonal capture antibody specific for Mouse IL-6 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Mouse IL-6 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinyla...
Catalog: KMC0062
Size: 192 tests
Category: Products & Services > Kits & Assays > RNA Amplification Kits
The Ambion® Illumina® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The new TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous Illumina® RNA Amplification Kit. Experience these Improvements: • Improved first-strand cDNA synthesis using ArrayScript™ reverse transcriptase • Biotinylated UTP allows ease of use and optimal labeling • Maximization of cRNA yield using MEGAscript® IVT technology • Signific...
Catalog: AMIL1791
Size: 24 reactions
Category: Products & Services > Kits & Assays > RNA Amplification Kits
The Ambion® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The kit includes sufficient reagents for 96 reactions. • Perform amplification from just 50 ng of input RNA • Developed for expression profiling using the Sentrix® BeadChips • Bio-16-UTP now included in optimized NTP mix, reducing setup time and maximizing labeling The TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous TotalPrep™ Kit. The IVT...
Catalog: 4393543
Size: 96 reactions
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Iscove's (IMDM)
Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This IMDM is modified as follows: With Without • L-glutamine • Phenol Red • α-thioglycerol • 2-mercaptoethanol
Catalog: 21056-023
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is pr...
Catalog: Q601-20
Size: 20 each
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is pr...
Catalog: Q611-20
Size: 20 each
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Insulin, Transferrin, & Iron Supplements
Gibco® Insulin, Transferrin, Selenium, Sodium Pyruvate Solution (ITS -A) is used as a basal medium supplement in order to reduce the amount of Fetal Bovine Serum (FBS) used to culture the cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G is now routinely used with other basal media to support a wide variety of cell types in the presence of 2-4% FBS. Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxyge...
Catalog: 51300-044
Size: 10 ml
Category: Products & Services > Labeling & Detection Products > Gel & Membrane Stains
InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you'll: Stain proteins fused to an oligohistidine sequence Eliminate western blotting-detect His-tagged proteins directly in the gel Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner Visualize results in under 3 hours Convenienty provided as one ready-to-use solution, InVision™ His-tag ...
Catalog: LC6030
Size: 500 ml