Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
Platinum® Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments where high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´a5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase (Table 1). PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. With Platinum® Taq High Fidelity you'll get: Greater than six times higher fidelity than Taq DNA poly-merase Amplification of fragments up to 15 kb (Figure 1) Room temperature reaction...
Catalog: 11304-102
Size: 5000 reactions
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
The pLenti6.2⁄V5-DEST™ Gateway® Vector is a Gateway®-adapted ViraPower™ II lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker. Advantages • Stable expression • Long-term experiments • High-throughput screening • Accurate titer of functional virus (using Blasticidin method) • Flexible and versatile Gateway® recombination cloning t...
Catalog: V368-20
Size: 6 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway® Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (catalog K5330-00). The pLenti6.3⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Poly...
Catalog: V533-06
Size: 1 kit
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
Invitrogen's™ pLenti6.3⁄V5™-TOPO®Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00). The pLenti6.3⁄V5™-TOPO® -TA Cloning Kit contains the TOPO®- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5™-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequenc...
Catalog: K5315-20
Size: 1 kit
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
The pLenti6⁄V5 Directional TOPO® Cloning Kit contains the TOPO®-adapted ViraPower™ lentiviral expression vector, pLenti6⁄V5-D-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving high-level, constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells. Advantages • High efficiency and rapid cloning • Constitutive gene expression in dividing and non-dividing mammalian cells in vitro or in vivo • Produces replication-incompet...
Catalog: K4955-10
Size: 20 reactions
Category: Products & Services > Buffers & Chemicals > Detergents & Solubilizers
Pluronic F-127 *20% solution in DMSO* is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media. Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of our ion indicators as well as our cell tracer dyes such as CFDA-SE.
Catalog: P-3000MP
Size: 1 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents
PLUS™ Reagent is used in conjunction with transfection reagents, such as Lipofectamine™, to enhance transfection in adherent cell lines. Some customers find when using Plus™ Reagent that less optimization of DNA concentration is needed to achieve high transfection performance. The addition of Plus™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types (1). PLUS™ Reagent used with Lipofectamine™ Reagent has been demonstrated to improve the transfe...
Catalog: 11514-015
Size: 0.85 ml
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > RNAi Vectors
The Ambion® pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors-—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency. Features of the pMIR-REPORT™ miRNA Expression Reporter Vector System: • Clone miRNA targets and evaluate miRNA regulation • Screen putative miRNA target sequences • Includes both reporter and control vectors The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell mea...
Catalog: AM5795M
Size: 1 kit w⁄ Manual
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
In addition to the original pcDNA™5/FRT vector, four more Flp-In™ expression vectors-pcDNA5/FRT/V5-His-TOPO®, pSecTag/FRT/V5-His-TOPO®, pEF5/FRT/V5-DEST™, and pEF5/FRT/V5-D-TOPO® (Figure 1)-are available for cloning and expressing in the Flp-In™ System. These vectors offer a variety of cloning options and different promoter types. Vectors include the following features: Flp Recombinase Target (FRT) site for efficient integration into Flp-In™ Cell Lines Hygromycin resistance gene for convenient selection of integrants C-terminal V5 tag for easy detection of fusion proteins with the Anti-V5 Anti...
Catalog: V6005-20
Size: 20 µg
Category: Products & Services > Instruments, Software, & Accessories > Capillaries & Polymers > Polymers
POP-6™ is a DNA sequencing and fragment analysis reagent and consumable. It is a separation matrix for performing fragment analysis and sequencing on the 3100 Genetic Analyzer and 3100-Avant™ Genetic Analyzer. The POP-6™ Polymer is ideally suited for standard and rapid sequencing. Many Choices For the ABI PRISM® 310 and 3100 Genetic Analyzers, POP-4® polymers are designed for microsatellite and other genotyping applications, while POP-6™ polymers are the choice for standard and rapid sequencing. For the ABI PRISM® 3700 DNA Analyzer, POP-5™ polymer is the separation matrix of choice for high-th...
Catalog: 4316357
Size: 7000 µl
Category: Products & Services > Instruments, Software, & Accessories > Capillaries & Polymers > Polymers
POP-6™ is a denaturing polymer used on the 310 Genetic Analyzer. POP-6™ Polymer is used for sequencing applications. • Easy to Use: Pre-formulated, which not only saves time but also saves reproducibility by ensuring consistent formulation. • Robust Formulation: Formulated for sequencing and fragment analysis applications. • Replenishable: Allow capillaries to be used multiple times. Reproducible Results POP™ polymers dynamically coat the capillary wall to control electro-osmotic flow. Their definite quality and uniform consistency eliminate guesswork and help to ensure reproducibility. POP Po...
Catalog: 402837
Size: 3 ml
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits
Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR • The optimized formulation contains highly purified AmpliTaq Gold® D...
Catalog: 4367659
Size: 200 reactions
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qRT-PCR Kits
Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-C T ™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes: • AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA • An additive that red...
Catalog: 4391178
Size: 40 reactions
Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Adhesive Film & Other Plate-Related Accessories
This precision plate holder is for use with Applied Biosystems® 7300 real-time PCR systems (SN 273000878 and above), as well as 7500 real-time PCR systems (SN 275001330 and above). Customers with earlier serial numbers should contact Product Support .
Catalog: 4367032
Size: 1 each
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
These vectors are designed for high-level, constitutive expression from either the CMV or RSV promoters. Both vectors contain the EBNA-1 gene for episomal expression in primate and canine cell lines.
Catalog: V004-50
Size: 20 µg
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Catalog: 4318930
Size: 1 kit
Category: Products & Services > Oligos, Primers, Probes & Nucleotides
FLO (50 NMOL)
Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software
The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination. • Enables individual oligonucleotide design for real-time PCR applications. • Supports assays based o...
Catalog: 4363991
Size: 1 license
Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software
The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination. • Enables individual oligonucleotide design for real-time PCR applications. • Supports assays based o...
Catalog: 4363993
Size: 1 license
Category: Products & Services > Labeling & Detection Products > Gel & Membrane Stains
Pro-Q Diamond phosphoprotein gel stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or phosphoprotein specific antibodies and Western blot analysis. Preparation of the Pro-Q Diamond phosphoprotein gel destaining solution is described in the gel staining protocol, but we also provide the detaining solution premixed for your convenience.
Catalog: P-33310
Size: 1 L
Category: Products & Services > Labeling & Detection Products > Gel & Membrane Stains
Molecular Probes' proprietary Pro-Q Emerald 300 and Pro-Q Emerald 488 Glycoprotein Stain Kits provide the most advanced technology available for detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. In less than three hours, it is possible to detect as little as 300 pg of glycoprotein per band, depending on the degree of glycosylation, making these stains at least 50-fold more sensitive than standard fuchsin staining.
Catalog: P-20495
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
Propidium iodide is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.
Catalog: P3566
Size: 10 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Detection Kits & Reagents > Protein A
Protein A from Staphylococcus aureus, Cowan I Strain conjugated with highly purified horseradish peroxidase (RZ > 3.0). The product is liquid containing 10 mM phosphate, 0.15 M NaCl buffer, pH 7.4, 1% ovalbumin, 40% glycerol, and 0.1% proclin as preservative.
Catalog: 10-1023
Size: 3 mg
Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
The Protein Quant Sample Lysis Kit contains the reagents required for the preparation of target and control cell lysates for use with TaqMan® Protein Assay experiments. It contains a one-step lysis solution for preparing crude cell lysates from cultured cells, and the lysates are compatible with cell counting as well as total protein quant measurements such as a microBCA assay. The Protein Quant Sample Lysis Kit contains a buffered nonionic detergent solution for the gentle lysis of adherent and non-adherent cultured cells under non-denaturing conditions
Catalog: 4448536
Size: 25 ml
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Protein Analysis Kits
Applied Biosystems® Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest. Save Time and Money with Your Sample Screening This solution significantly reduces the time and cost associated with screening samples relative to currently available methods. Protein Thermal Shift™ products that are run on our real-time PCR sys...
Catalog: 4462263
Size: 2000 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Proteases & Peptidases
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g. the presence of SDS or urea) • In the presence of metal chelating agents (e.g. EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization. Performance and Quality Testing: End...
Catalog: 4333793
Size: 5 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Proteases & Peptidases
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g. the presence of SDS or urea) • In the presence of metal chelating agents (e.g. EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization. Performance and Quality Testing: End...
Catalog: AM2546
Size: 1.25 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Proteases & Peptidases
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g. the presence of SDS or urea) • In the presence of metal chelating agents (e.g. EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization. Performance and Quality Testing: End...
Catalog: AM2548
Size: 5 x 1.25 ml
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Catalog: K6025-01
Size: 20 reactions
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features: Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2) Cytomegalovirus (CMV) promoter for high-level constitutive expression C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chel...
Catalog: V900-20
Size: 20 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Yeast Expression Vectors
pTEF1/Zeo is a 3.6 kb vector designed to allow you to create your own cloning and/or expression vectors that confer resistance to Zeocin™ in both E. coli and yeast strains. The vector contains the Sh ble Zeocin™ resistance gene driven by the Saccharomyces cerevisiae TEF1 promoter for selection in any yeast. In addition, the synthetic EM-7 bacterial promoter is present for selection in E. col i. Several unique restriction sites flank the ZeoCassette™ region to allow easy subcloning of the region or cloning of additional elements into pTEF1/Zeo. This vector is also useful for generating gene dis...
Catalog: V503-20
Size: 20 µg
Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Isolation Accessories
The PureLink® Air Porous Tape is a component in the PureLink® 96 Plasmid Purification System. The PureLink® 96 Plasmid Purification offers a method for high throughput plasmid DNA isolation. The PureLink® Air Porous Tape allows for proper aeration of bacterial cultures.
Catalog: 12262-010
Size: 50 pieces
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Modifying Enzymes
The PureLink® DNase Set provides rapid and efficient removal of DNA from RNA that has been purified using PureLink® RNA kits. The PureLink® DNase Set is: • RNase-free for use in RNA purification • Optimized for on-column digestion • Lyophilized for stability The RNase-free DNase set comes lyophilized and is optimized for on-column digestion of DNA using PureLink® protocols. The degree of DNA digestion performed by PureLink® DNase is optional for most PureLink® RNA kits, due to the efficient removal of the majority of DNA by the PureLink® technology; however, some downstream applications that a...
Catalog: 12185010
Size: 50 preps
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Plasmid DNA Purification Kits
The PureLink® HiPure Plasmid Purification Systems are designed to isolate transfection-grade plasmid DNA from E. coli in less than 2 hours. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 ml bacterial culture, at a purity that is comparable to that achieved with two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include: • Labor- and time-saving —No need for extra steps to remove endotoxin and other contaminants • High purity, low endotoxin product —pure enough for mammalian cell transfection • Versatility —p...
Catalog: K2100-07
Size: 25 preps
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Plasmid DNA Purification Kits
Fast, simple protocols - Purify your plasmid DNA in less than 2 hours Labor-saving - No need for extra steps to remove endotoxin and other contaminants Megapreps and Gigapreps save time by utilizing vacuum-assisted cartridges PureLink® HiPure Plasmid Purification Systems are designed to isolate plasmid DNA of the highest purity (1) with a wide scale range (Table 1). Simple protocols an anion-exchange resin allow you to purify plasmid DNA (Figure 1) at a quality equivalent to two passes through cesium chloride gradients - the most rigorous method for DNA purification. In less than 2 hours, DNA ...
Catalog: K2100-08
Size: 4 preps
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > PCR Clean-Up Kits
PureLink® PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products (Figure 1). The simple PureLink® bind/wash/elute procedure: provides two Binding Buffer options for fragments >100 bp or >300 bp* can be performed in either single column or 96-well plate format takes Two proprietary buffers are supplied to meet the needs of different purification requirements. Use the Binding Buffer for routine purifications of double-stranded DNA fragments from 100 bp to 12 kb. Use Binding Buffer HC for removal of primer...
Catalog: K3100-01
Size: 50 reactions
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Gel Extraction Kits
Successfully purify DNA fragments from 40 bp to 10 kb Proven purity made possible using traditional silica-based technology The PureLink® Gel Extraction Kit is designed to purify DNA fragments from agarose gels (1). The simple procedure uses a silica-based spin cartridge (Figure 1) to purify DNA fragments from 40 bp to 10 kb in in vitro transcription, restriction mapping, cloning, and labeling.
Catalog: K2100-12
Size: 50 preps
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. • Rapid, Non-Hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and Convenient Column Purification Procedures The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and t...
Catalog: 12183018A
Size: 50 preps
Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Other Tubes
The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Catalog: 12282-100
Size: 100 tubes
Category: Products & Services > Antibodies & Secondary Detection Reagents > Isotype Antibody Controls
Catalog: 02-9602
Size: 5 mg
Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics
Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger . Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the "pac" gene. Puromycin is widely used in cell biology as a selective agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2 – 5.0 µg/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/ml. Gibco® Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer. Life Technologies offer...
Catalog: A11138-02
Size: 20 ml
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Blotting Membranes > PVDF Membranes
Invitrogen makes blotting easier by providing a variety of pre-cut, pre-assembled membrane/filter-paper sandwiches. A protein's properties ( i.e. , charge, hydrophobicity, etc.) affect its ability to bind to membrane surfaces. Finding the right membrane may require experimenting with your specific protein on different membranes. Store all membranes at room temperature.
Catalog: LC2002
Size: 20⁄pack
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Yeast Expression Vectors
The pYES2 vector is designed for native expression of your protein of interest in S. cerevisia e. It contains the URA3 gene for selection in yeast and 2µ origin for high-copy maintenance.
Catalog: V825-20
Size: 20 µg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Amine-Reactive Fluorophores, Biotins, Quantum Dots, & Other Labels
The amine-reactive PyMPO succinimidyl ester can be used to create bioconjugates with this environment-sensitive fluorophore.
Catalog: S-6110
Size: 5 mg
Category: Products & Services > Labeling & Detection Products > BioConjugates > Lectins
Wheat germ agglutinin (WGA) is one of the most widely used lectins in cell biology. Our Qdot(R) 655 conjugate of WGA exhibits the extremely bright and photostable fluorescence of the Qdot® 655 nanocrystal. Qdot® 655 WGA binds to sialic acid and N-acetylglucosaminyl residues.
Catalog: Q12021MP
Size: 200 µl
Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits
Using the PicoGreen® dsDNA quantitation assay, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides (1). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. PicoGreen® dsDNA Quantitation Reagents are: Orders of magnitude more sensitive than UV absorbance readings, saving on precious sample (Figure 1) Specific for dsDNA in the presence of equimolar amounts of RNA (Figur...
Catalog: P7581
Size: 1 ml
Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins
This is the user's manual for the Quantifiler® Duo DNA Quantification Kit. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4391294
Size: 1 manual
Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Quantification Kits
This real-time PCR-based kit is designed for the quantification of amplifiable human DNA that is present in a sample, and has been validated for human identification applications. An internal PCR control is formulated into each reaction to identify samples that may contain PCR inhibitors. • Quantifies DNA from a wide variety of sources including buccal swabs, blood, semen, tissue, and other human DNA sources making this kit suitable for casework, databasing, and paternity labs. • Chemistry compatible with commonly used extraction technologies for easy integration into current lab workflow. • P...
Catalog: 4343895
Size: 400 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Real-Time PCR Instruments
The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research. The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setu...
Catalog: 4471134
Size: 1 system
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Ambion® Quantu mRNA ™ 18S Internal Standard with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal 18S primers that cannot be extended. By adjusting the ratio of 18S Competimer™s to normal 18S mRNA primers, the signal for 18S mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. Because of its invariant expression across tissues and treatments, 18S ribosomal RNA is an ideal internal control for quantitative RNA analysis. The Universal 18S Inte...
Catalog: AM1718
Size: 100 reactions