Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Mouse anti-phospho-Tau (Thr231); Unconjugated Monoclonal antibody specific to Human Tau [pT231]. This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-Tau [pT231] recognizes the expressed product of the MAPT gene.
Catalog: 35-5200
Size: 50 µg
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores
The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (K d ) of fluorescent Ca 2+ indicators using a fluorometer or quantitative imaging system. The Kd of an indicator can be calculated from a plot generated by scanning the excitation or emission of the indicator in the presence of eleven different Ca 2+ concentrations. Calcium Calibration Kit #1 contains 50 mL of 10 mM K 2 EGTA and 50 mL of 10 mM CaEGTA. Both solutions contain 100 mM KCl and 30 mM MOPS, pH 7.2 and are prepared in deionized water. These stock solutions can be blended to make buffers havin...
Catalog: C-3008MP
Size: 1 kit
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits
The TaqMan® Fast Reagents Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in cDNA samples using the TaqMan 5’ nuclease assay, in fast thermal cycling mode. The Eukaryotic 18S rRNA TaqMan® Assay consists of a 20X mix of two unlabeled PCR primers and a FAM™ dye labeled 18S TaqMan® MGB probe. Used With: 7900HT Fast Real-Time PCR System with Fast 96-Well Block Module, 7900HT Fast Real-Time Service Upgrade, 7900HT System Fast 96-Well Block Upgrade Kit. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4352407
Size: 125 reactions
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Metabolic Assay Kits
The Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit contains a sensitive, one-step assay that uses the Amplex® Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) to detect hydrogen peroxide (H 2 O 2 ) or peroxidase activity. The Amplex® Red reagent, in combination with horseradish peroxidase (HRP), has been used to detect H 2 O 2 released from biological samples, including cells, or generated in enzyme-coupled reactions. Furthermore, Amplex® Red reagent can be used as an ultrasensitive assay for peroxidase activity when H 2 O 2 is in excess. In the presence of peroxidase, the Amplex® Red reag...
Catalog: A22188
Size: 1 kit
Category: Products & Services > Controls, Standards & Ladders > DNA Control Probes
Applications: ISH
Catalog: Q151P.0100
Size: 0.5 ml
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Cap Analog & Variants
Ambion® Cap Analog [m 7 G(5')ppp(5')G] is used for the synthesis of 5' capped RNA molecules in in vitro transcription reactions. These molecules are translated more efficiently in in vitro translation systems than uncapped mRNAs. 25 A 254 U supplied. Substitution of cap analog for a portion of the GTP in an in vitro transcription reaction results in the incorporation of the cap structure into a corresponding fraction of the transcripts. Capped mRNAs are generally translated more efficiently in reticulocyte lysate and wheat germ in vitro translation systems. It is important that in vitro transc...
Catalog: AM8050
Size: 1 tube
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2925
Size: 1 mg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2927
Size: 1 mg
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Plasmid DNA Purification Kits
Fast, simple protocols - Purify your plasmid DNA in less than 2 hours Labor-saving - No need for extra steps to remove endotoxin and other contaminants Megapreps and Gigapreps save time by utilizing vacuum-assisted cartridges PureLink® HiPure Plasmid Purification Systems are designed to isolate plasmid DNA of the highest purity (1) with a wide scale range (Table 1). Simple protocols an anion-exchange resin allow you to purify plasmid DNA (Figure 1) at a quality equivalent to two passes through cesium chloride gradients - the most rigorous method for DNA purification. In less than 2 hours, DNA ...
Catalog: K2100-08
Size: 4 preps
Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells
MAX Efficiency® Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to rec A1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences (1) and tandem array genes (2). MAX Efficiency® Stbl2™ Competent Cells offer: >1 x 10 9 transformants/flg for cloning rare sequences Elimination of mcr BC- hsd RMS- mrr and mutation of mcr A allows cloning of methylated genomic sequences (1) Unique genotype stabilizes direct repeat and retroviral sequences such as SIV and HIV (1) Greatly ...
Catalog: 10268-019
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Mouse anti-Maltose Binding Protein; Unconjugated Monoclonal antibody specific to Multiple Maltose Binding Protein. This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-Maltose Binding Protein recognizes the expressed product of the MBP gene.
Catalog: 33-5100
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
CD32, Mouse Anti-Human, (R-PE); R-PE conjugated Monoclonal antibody specific to Human CD32. This antibody is validated for use in Flow Cytometry. Anti-CD32 recognizes the expressed product of the FCGR2A gene.
Catalog: CD3204
Size: 0.5 ml
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels
Conjugates prepared with the thiol-reactive Alexa Fluor 488 C 5 maleimide are significantly brighter and more photostable than fluorescein. The bright, green fluorescence of the Alexa Fluor 488 dye, which is spectrally similar to that of fluorescein, is pH independent from pH 4 to 10.
Catalog: A-10254
Size: 1 mg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels
The thiol-reactive Alexa Fluor 546 C 5 maleimide and its conjugates are perfect for applications that require orange-fluorescent probes. Spectrally similar to the Cy3 dye, the Alexa Fluor 546 dye is readily excited by the 546 nm line of the mercury-arc lamp and the 543 nm line of the He-Ne laser.
Catalog: A-10258
Size: 1 mg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels
Conjugates prepared with the thiol-reactive Alexa Fluor 647® C 2 maleimide are spectrally similar to the Cy5 dye, resulting in an optimal match to optical filters for the Cy5 dye. Total fluorescence of secondary antibody conjugates of Alexa Fluor® 647 dye, however, is significantly higher than that of Cy5 conjugates. Unlike the Cy5 dye, Alexa Fluor® 647 dye has very little change in absorption of fluorescence spectra when conjugated to most porteins, oligonucleotides, or nucleic acids, yielding greater total fluorescence at the same degree of substitution.
Catalog: A-20347
Size: 1 mg
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Reactive Oxygen & Nitrogen Species
MitoSOX™ Red reagent is a novel fluorogenic dye specifically targeted to mitochondria in live cells. Oxidation of MitoSOX™ Red reagent by superoxide produces red fluorescence. • Readily oxidized by superoxide but not by other ROS- or RNS-generating systems • Absorption/emission maxima: ~510/580 nm • Use for live cell imaging • Rapidly and selectively targeted to the mitochondria Quick, Easy Detection of Mitochondrial Superoxide in Live Cells The production of superoxide by mitochondria can be visualized in fluorescence microscopy using the MitoSOX™ Red reagent. MitoSOX™ Red reagent permeates l...
Catalog: M36008
Size: 10 × 50 µg
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards
The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: 4404312
Size: 4 tubes
Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Other Enzyme Assay Kits
The EnzChek® Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme acitivty in solution. The assay measures lysozyme activity on Micrococcus lysodeikticus cell walls, which are labeled to such a degree with fluorescein that the fluorescence is quenched. Lysozyme action can relieve this quenching, yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.
Catalog: E22013
Size: 1 kit
Category: Products & Services > Competent Cells & Strains > Other Competent Cells & Strains
The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA str...
Catalog: C1010-10
Size: 10 reactions
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need—including your choice of antibiotics and optional X-gal/IPTG—to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a...
Catalog: Q611-20
Size: 20 each
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need—including your choice of antibiotics and optional X-gal/IPTG—to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a...
Catalog: Q601-20
Size: 20 each
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. • Rapid, Non-Hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and Convenient Column Purification Procedures The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and t...
Catalog: 12183018A
Size: 50 preps
Category: Products & Services > Instruments, Software, & Accessories > Imaging & Microscopy Accessories > Microscopy Accessories
ARCTURUS® laser capture microdissection (LCM) instruments offer a unique combination of infrared (IR) laser capture and ultraviolet (UV) laser cutting, permitting the use of any slide type and any sample preparation for microdissection. While plain glass slides are effectively used for standard IR-based LCM, polyethylene naphthalate (PEN) membrane slides facilitate this Arcturus®-exclusive dual laser function and allow you to determine the appropriate sample preparation based on your application need. Plain Glass Slides The ARCTURUS® instruments are the only LCM systems that allow efficient us...
Catalog: LCM0522
Size: 50 each
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin
Gibco® Trypsin 1:250 powder is an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging, and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing Quality Testing Gibco® Trypsin 1:250 is tested for endotoxin, mycoplasma, bacterial, fungal and viral contamination. Other quality tests include multiple activity assays, ash analysis and mois...
Catalog: 27250-018
Size: 100 g
Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells
TOP10 E. coli are provided at a transformation efficiency of 1 x 10e 9 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids. The genotype of TOP10 cells is similar to the DH10B™strain, and offers the following features: -hsd R for efficient transformation of unmethylated DNA from PCR amplifications -mcrA for efficient transformation of methylated DNA from genomic preparations -lacZΔM15 for blue/white color screening of recombinant clones -endA1 for cleaner preparations of DNA and better results in d...
Catalog: C4040-10
Size: 10 reactions
Category: Products & Services > Instruments, Software, & Accessories
Catalog: 4305936
Size: 500 tubes
Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells
One Shot® ccd B Survival™ 2 T1 Phage-Resistant (T1 R ) chemically competent E. coli are suitable for the propagation of plasmids containing the ccd B gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and super-coiled entry vectors. Transformation efficiency is >1 × 10 9 transformants/ µg DNA. The ccd B Survival™ 2 T1 R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1 R E. coli strain offers the following Features: • Resistance to the ccd B gene product • Resistance to T1 and T5 phage (the ton A/ fh...
Catalog: A10460
Size: 10 transformations
Category: Products & Services > Instruments, Software, & Accessories > Capillaries & Polymers > Polymers
POP-6™ is a denaturing polymer used on the 310 Genetic Analyzer. POP-6™ Polymer is used for sequencing applications. • Easy to Use: Pre-formulated, which not only saves time but also saves reproducibility by ensuring consistent formulation. • Robust Formulation: Formulated for sequencing and fragment analysis applications. • Replenishable: Allow capillaries to be used multiple times. Reproducible Results POP™ polymers dynamically coat the capillary wall to control electro-osmotic flow. Their definite quality and uniform consistency eliminate guesswork and help to ensure reproducibility. POP Po...
Catalog: 402837
Size: 3 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Modifying Enzymes
Ambion® RNase Cocktail™ is a mixture of two highly purified ribonucleases, RNase A (500 U/mL) and RNase T1 (20,000 U/mL), and is free of DNase and nicking activities. Use RNase Cocktail™ for all situations where it is desirable to degrade RNA, i.e. plasmid minipreps and ribonuclease protection assays. Supplied in five tubes containing 1 mL each. Digestion of RNA with RNase A alone leaves fragments of RNA that are large enough to be visible on agarose gels and precipitate in ethanol. RNase A cuts after C and U residues, and RNase T1 cuts after G residues. Consequently, the mixture of both enzym...
Catalog: AM2288
Size: 5 tubes
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Aldehyde-, Ketone-, & Carboxylate-Reactive Labels
The Alexa Fluor 488 hydrazide is a low molecular weight, cell membrane—impermeant, aldehyde-fixable molecule that can be used as a cell tracer. This green-fluorescent polar dye can be loaded into cells by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: A-10436
Size: 1 mg
Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Beta-Galactosidase Substrates
Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, first to fluorescein monogalactoside (FMG) and then to highly fluorescent fluorescein. Enzyme-mediated hydorlysis of FDG can be followed by the increase in either absorbance or fluorescence.
Catalog: F-1179
Size: 5 mg
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores
Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pK a of 4.8, this dye can be used as an acidic pH sensor.
Catalog: C-369
Size: 100 mg
Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards
AB-Human Serum (heat-inactivated)
Catalog: 34005-100
Size: 100 ml
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > DNA Subcloning Vectors
Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B™ E. coli , also has 14 non-selectable unique restriction endonuclease recognition sites. Unique cleavage sites for Hin d III and Cla I are found in the promoter of the tetracycline resistance gene. Insertion of DNA at either of these two sites usually results in loss of tetracycline resistance. Performance and Quality Testing: Purity, DNA structure, and selected restriction endonuclease fragmentation...
Catalog: 15367-014
Size: 25 µg
Category: Products & Services > Kits & Assays > Impurity & Contamination Detection Kits > Mycoplasma Detection Kits
The MycoFluor™ Mycoplasma Detection Kit provides an ultrasensitive, rapid and simple fluorescence microscopic acid for the visual identification of mycoplasma infection in laboratory cell cultures. In order to detect mycoplasma, the fluorescent MycoFluor™ reagent is added directly to the culture medium, with or without cells, and the stained sample is then examined under a fluorescence microscope.
Catalog: M7006
Size: 1 kit
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Epitope Tag & Reporter Specific Antibodies
This purified Mouse Anti-Xpress Monoclonal Antibody is designed to specifically interact with the Xpress™ leader peptide. The monoclonal antibody recognizes the amino acid sequence -Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys- . This Mouse Anti-Xpress™ Antibody has been tested for use in applications such as western blots, ELISA, and immunoprecipitation. Antibody Specifications: • Applications: Validated applications for Anti-Xpress™ Monoclonal Antibody are western blotting, immunoprecipitation, immunofluporescence, and ELISA. • Host Species and Isotype: The host species of the Xpress monoclonal antibody ...
Catalog: R910-25
Size: 50 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
Alexa Fluor® 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western ...
Catalog: S-32354
Size: 0.5 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • NEAA • L-glutamine • Phenol Red • HEPES
Catalog: 10370-088
Size: 10 × 500 ml
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
Alkaline Phosphatase Live Stain is a stem cell imaging product that allows users to differentially stain pluripotent stem cells (PSCs). The dye is a cell-permeable fluorescent substrate for alkaline phosphatase (AP) that is non-toxic to cells, diffusing out over the course of two hours. The Alkaline Phosphatase Live Stain consists of 50 µL of a 500X fluorescein-based dye in DMSO, sufficient for staining two 24-well plates, four 6 cm dishes or two 10 cm dishes. This Alkaline Phosphatase Live Stain allows you to: • Identify pluripotent stem cells with a strong signal above background • Stain PSC...
Catalog: A14353
Size: 50 µl
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides
BODIPY FL adenosine 5'-triphosphate (A-12410) and BODIPY FL guanosine 5'-triphosphate are composed of a BODIPY FL fluorophore attached to the 2' or 3' position of the ribose ring via a linker. Interactions between the fluorophore and the purine base are evident from the spectroscopic properties of these nucleotide analogs. The fluorescence quantum yield of BODIPY FL GTP is only about one-tenth that of BODIPY FL ATP; however, this yield may be enhanced upon binding of BODIPY FL GTP to GTP-binding proteins.
Catalog: G-12411
Size: 100 µl
Category: Products & Services > Controls, Standards & Ladders > Protein Standards & Ladders > Unstained Protein Standards
The HiMark™ Unstained High Molecular Weight (HMW) Protein Standard is designed for analysis of high molecular weight proteins on Novex® NuPAGE® Tris-Acetate Gels . • Comprehensive – Contains markers with a wide range of high molecular weights, providing 9 protein bands in the range of 30-460 kDa • Convenient – A ready-to-use formulation eliminates the need to heat, reduce, or add sample buffer prior to use • Compatible – Intended for use with NuPAGE® Novex 3-8% and 7% Tris-Acetate Gels under denaturing conditions* *Note: HiMark™ Unstained High Molecular Weight Protein Standard can also be used...
Catalog: LC5688
Size: 250 µl
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Collagenase
Collagenase is a protease with specificity for the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Glyc-Pro. This sequence is found in high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation has been suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. It is relatively gentle, dissociates well at physiologic...
Catalog: 17101-015
Size: 1 g
Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chromogens
TMB (Tetramethyl Benzidine) chromogen solution may be used with horseradish peroxidase (HRP)-based immunodetection procedures.
Catalog: 00-2019
Size: 500 ml
Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents
The MitoProbe™ DiIC 1 (5) Assay Kit provides solutions of the membrane-potential-sensitive cyanine dye DiIC 1 (5), and a mitochondrial membrane-potential disrupter, CCCP, for the study of mitochondrial membrane potential. DiIC 1 (5) penetrates the cytosol of eukaryotic cells and accumulates primarily in mitochondria with active membrane potentials, producing bright, far-red fluorescence. DiIC 1 (5) staining intensity decreases with decreased mitochondrial membrane potential.
Catalog: M34151
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2110
Size: 5 mg
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > F-12 Nutrient Mixture
Ham's F-12K (Kaighn's) medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) medium was developed for primary human hepatocytes, as well as some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) medium is manufactured as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 21127-030
Size: 10 × 500 ml
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels
Our 4-acetamido-4'-maleimidylstilbene-2,2'- disulfonic acid is a thiol-reactive reagent that is water soluble, with high polarity and membrane impermeability. This polysulfonated dye is useful for determining whether thiol-containing proteins and polypeptide chains are exposed at the extracellular or cytoplasmic membrane surface.
Catalog: A-485
Size: 25 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The IgG (γ), goat F(ab’), 2 R-phycoerythrin (R-PE) dye conjugate is a polyclonal goat antibody that is Fc specific and recognizes human IgG. R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence emission without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients. Use of R-PE in antibody conjugates results in probes with greatly enhanced detectability. Antibody Specifications Description: IgG (γ), Goat F(ab’)2 polyclonal, Fc specific Label: R-PE Validated Appl...
Catalog: H10104
Size: 0.5 ml
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Amine-Reactive Fluorophores, Biotins, Quantum Dots, & Other Labels
The amine-reactive Alexa Fluor 430 carboxylic acid, succinimidyl ester and its conjugates exhibit the rare combination of absorption between 400 nm and 450 nm and fluorescence emission beyond 500 nm (excitation/emission maxima ~434/539 nm).
Catalog: A-10169
Size: 5 mg
Category: Products & Services > Kits & Assays > Impurity & Contamination Detection Kits > Mycoplasma Detection Kits
The Discriminatory Positive⁄Extraction Control is a large plasmid containing a Mycoplasma DNA sequence. This control was designed to behave like Mycoplasma DNA in both the sample preparation and detection portions of the assay. Additionally, the DNA sequence has been modified so that the amplicon generated from this control has a melting temperature (Tm) of approximately 84°C, which is outside the range of amplicons generated from Mycoplasma with this assay. Thus, the Tm can be used to discriminate between a positive test result from Mycoplasma and the control DNA. This novel control enables r...
Catalog: 4445000
Size: 1 kit