Products from Taqman® Rnase P Control Reagents Kit to Taqman® Universal Pcr Master Mix, 1-Pack (1 X 5 Ml)

TaqMan® RNase P Control Reagents Kit

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The RNase P gene is a single-copy gene that encodes the RNA moiety for the RNase P enzyme. Applied Biosystems® TaqMan® RNase P Control Reagents were designed with limiting primer concentrations to be used as the endogenous reference in multiplex reactions. Multiplex PCR is the use of more than one probe and primer pair in the same tube. This kit is designed to perform a 5' nuclease assay with TaqMan® Universal PCR Master Mix using the provided human genomic DNA as template. • Pre-designed primers and TaqMan® probe eliminate assay design • Easy-to-use primer and TaqMan® probe mix minimizes assa...
Catalog: 4316844
Size: 1000 reactions

Dynabeads® Sheep Anti-Rat IgG

Category: Products & Services > Beads & Microspheres > Dynabeads® for Cell Isolation & Expansion > Dynabeads® for Other Cell Isolation

Dynabeads® Sheep-anti Rat IgG binds monoclonal rat IgGs of your choice for specific cell isolation or depletion. All cells from all species except from rat can be isolated with this product, depending only on the specific antibody of choice. Especially well suited for cell isolation from mouse samples. Depletion: cells can be depleted from a sample Positive isolation: cells can be positively isolated from a sample for molecular applications Negative isolation: cells can be negatively isolated from a sample (i.e. spleen or lymph node cells) by adding an antibody cocktail of rat IgGs towards the...
Catalog: 11035
Size: 5 ml

Eukaryotic 18S rRNA Endogenous Control (VIC®/MGB Probe, Primer Limited)

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Applied Biosystems® Eukaryotic 18S rRNA Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan Gene Expression Assays, Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: 18S RefSeq: X03205.1 Probe Exo...
Catalog: 4319413E
Size: 1000 reactions

Human ACTB (Beta Actin) Endogenous Control (VIC® / MGB Probe, Primer Limited)

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Applied Biosystems® Human ACTB (beta actin) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_0...
Catalog: 4326315E
Size: 1000 reactions

Human PGK1 (Phosphoglycerate Kinase 1) Endogenous Control (VIC® / MGB Probe, Primer Limited)

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Applied Biosystems® Human PGK1 (phosphoglycerate kinase 1) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: PG...
Catalog: 4326318E
Size: 1000 reactions

Mouse ACTB (Actin, Beta) Endogenous Control (VIC® / MGB Probe, Primer Limited)

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Applied Biosystems® Mouse ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: Actb Re...
Catalog: 4352341E
Size: 1000 reactions

Rat ACTB (actin, beta) Endogenous Control (VIC®/MGB Probe, Primer Limited)

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Applied Biosystems® Rat ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: ACTB RefSeq: NM_031144.2 Probe Exon Location...
Catalog: 4352340E
Size: 1000 reactions

Rat GAPD (GAPDH) Endogenous Control (VIC®/MGB Probe, Primer Limited)

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Applied Biosystems® Rat GAPD (GAPDH) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: GAPDH RefSeq: NM_017008.3 Probe Exon Location: 3 Ampl...
Catalog: 4352338E
Size: 1000 reactions

IFN-β Mouse ELISA Kit

Category: Products & Services > Kits & Assays > ELISA Kits > ELISA

The IFN-β Mouse ELISA Kit quantifies natural and recombinant mouse IFN-β in serum and cell culture supernatant. Interferon-β (IFN-β) is a cytokine that is produced by the cells of the immune system.
Catalog: KMC4041
Size: 96 tests

RNase Zap ®

Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > Nuclease Control Reagents

Ambion® RNaseZap® is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® onto the surface to be decontaminated and rinse it off with RNase-free water. Supplied in a single 4 liter cubitainer. • Completely removes RNase contamination from glass and plastic surfaces • Excels at removing high levels of RNase contamination whereas similar products fail • Proven effective at removing high concentrations of dried-on RNase A • Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free Working with RNA requires that special measures be...
Catalog: AM9784
Size: 4 L

Sequencing Analysis Software v 5.4 Upgrade from v5.3.1

Category: Products & Services > Instruments, Software, & Accessories > Software > Sequencing & Genetic Analysis Software

This product can only be used to upgrade Sequencing Analysis Software v5.3.1 installed on standalone computers, computers separate to the instrument data collection system. Please talk with your representative to upgrade the data collection system and secondary analysis software running on the data collection computer. Sequencing Analysis Software enables you to basecall, trim, display, edit, and print data from our entire line of capillary DNA sequencing instruments for data analysis and quality control. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4310991
Size: 1 CD

pcDNA™5/FRT Vector

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

pcDNA5⁄FRT vector is a 5.1 kb expression vector designed for use with the Flp-In™ System. This vector features: 1. CMV promoter for high level expression in mammalian cells; 2. ten unique restriction site for easy cloning; 3. FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ host cell line and 4. Hygromycin resistance gene for selection of stable cell lines.
Catalog: V6010-20
Size: 20 µg

NA-Fluor™ Influenza Neuraminidase Assay Kit

Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Other Enzyme Assay Kits

The NA-Fluor™ Influenza Neuraminidase Assay Kit provides validated reagents and a standardized protocol for conducting neuraminidase enzyme assays, including neuraminidase inhibitor (NI) susceptibility screening using a fluorescent MUNANA substrate. Key product features: • Efficient design—neuraminidase assay in one complete kit • Optimized formulations—assay reagents have been optimized for maximum performance based on NISN protocols • Robust application—functional detection of NI-resistant virus in mixed viral populations • Easy of use & flexible screening—stable fluorescent signal enables b...
Catalog: 4457091
Size: 960 assays

pcDNA™3.1/Zeo (+)

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

This pcDNA™3.1/Zeo (+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a forward-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+) or ...
Catalog: V860-20
Size: 20 µg

pcDNA™3.1 (+)

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

This pcDNA™3.1 (+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+) or r...
Catalog: V790-20
Size: 20 µg

EGFr Mouse Monclonal Antibody (clone 31G7), Concentrate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

This monoclonal antibody reacts with the extracellular domain of epidermal growth factor receptor (170 kDa). Clone 31G7 is useful in detecting the majority of squamous cell carcinomas that arise from both squamous epithelium and metaplastic squamous epithelium. Positive tissue: Breast carcinoma. Clone/PAD: 31G7 Immunogen: EGFr derived from A-431 cells Specificity: EGFr. No cross-reactivity observed with c-erbB-2. Reactivity: Human Applications: Immunohistochemistry (FFPE) Pretreatment: Epitope Retrieval: Not required , Enzyme Digestion: Required (Digest-All™ 3/Pepsin) Expected Staining Pattern...
Catalog: 28-0005
Size: 2 ml

DynaMag™-2 Magnet

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers

The new DynaMag™-2 magnet is optimized for efficient magnetic separation in small sample volumes using Dynabeads®. The DynaMag™-2 magnet combines a strong magnetic attraction for efficient separations with a flexible and smart ergonomic design. The top rack can be easily removed from the magnet in the base, ready for resuspension, vortexing, rotation or manual sample shaking. A center pin in the rack ensures equal vortexing of all tubes. The rack can also be removed and used to store tubes. The magnet can be used with all types of Dynabeads® for efficient isolation of proteins and nucleic acid...
Catalog: 12321D
Size: each

GeneScan™ 1200 LIZ® Size Standard

Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > Fragment Analysis Size Standards

The GeneScan™ 1200 LIZ® Size Standard is a fifth dye (LIZ®) labeled high density size standard for the reproducible sizing of fragment analysis samples. The standard contains 68 LIZ® dye-labeled, single-stranded DNA fragments. Since the standard is labeled with the fifth dye, users can genotype a greater number of markers in a given lane, compared to the four-dye system. GeneScan™ 1200 LIZ® Size Standard is designed for sizing DNA fragments in the 20-1200 nucleotides range and provides 68 single-stranded labeled fragments of: 20, 30, 40, 60, 80, 100, 114, 120, 140, 160, 180, 200, 214, 220, 240...
Catalog: 4379950
Size: 800 reactions

NativePure™ pcDNA™ Gateway® Vector Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The NativePure™ Mammalian Affinity Purification Kit provides an easy-to-use, efficient method for purifying native protein complexes. NativePure™ pcDNA™ Gateway® vectors (Figure 1) are designed to allow expression of biotinylated recombinant fusion proteins in mammalian cells through N- or C- terminal fusion of your recombinant protein of interest to the capTEV™ Tag. The capTEV™ Tag consists of a BioEase™ in vivo biotinylation peptide, two Tobacco Etch Virus (TEV) protease recognition sites, and a 6XHis tag. The BioEase™ tag, a 72-amino acid sequence from K. pneumoniae , directs in vivo biotin...
Catalog: BN3002
Size: 1 kit

APO BrdU (TUNEL) KIT

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Apoptosis Kits

The ApoTarget™ APO-BRDU Kit is a two-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry.
Catalog: KHO1001
Size: 60 tests

NuPAGE® MOPS SDS Running Buffer (20X)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Running Buffers

NuPAGE® MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE® Novex® Bis-Tris gels only. NuPAGE® MOPS SDS Running Buffer is recommended for separating small- to medium-sized proteins. Use the right buffer to optimize protein separations NuPAGE® MES SDS Running Buffer and NuPAGE® MOPS SDS Running Buffer can both be used with NuPAGE® Novex® Bis-Tris gels. Use of MOPS buffer allows proteins to run slower than when using MES buffer. Get consistent, convenient, high-quality results Pre-mixed NuPAGE® buffers are a convenient way to ensure high-quality, consistent electrophoresi...
Catalog: NP0001-02
Size: 5 L

pCMV myc /nuc

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The pShooter™ vectors are designed to localize recombinant proteins to the nucleus, mitochondria, endoplasmic reticulum, or cytoplasm. In addition to the localization signal, each pShooter™ vector contains: Strong mammalian promoter (CMV or EF-1É) for constitutive expression of your protein of interest C-terminal c- myc epitope for detection with an Anti- myc Antibody Bovine Growth Hormone (BGH) polyadenylation site for mRNA stability f1 origin for single-stranded rescue of sense DNA Neomycin resistance gene for selection of mammalian cells with Geneticin¤ selection agent Ampicillin resistance...
Catalog: V821-20
Size: 20 µg

Custom Plus TaqMan® RNA Assay, MED

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

Custom Plus TaqMan® RNA Assay, MED
Catalog: 4441117
Size: 750 µl

Dispase II, powder

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Other Cell Dissociation Reagents

Dispase II (neutral protease) is an amino-endo peptidase that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. This sequence is found with high frequency in collagen. Dispase II has a mild proteolytic action that makes it useful for the isolation and routine passaging of primary cells. Dispase II is relatively gentle, dissociates well at physiological temperature and pH, and generally maintains cell membrane integrity. Gibco® Dispase II is produced in Bacillus polymyxa and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation, an...
Catalog: 17105-041
Size: 5 g

IgG Subclass Human ELISA Kit

Category: Products & Services > Kits & Assays > ELISA Kits > ELISA

The IgG Subclass Human ELISA Kit profile contains components required to construct an enzyme-linked immunoassay for the specific and quantitative measurement of human IgG1, IgG2, IgG3, and IgG4 subclasses.
Catalog: 99-1000
Size: 192 tests

TaqMan® MGB Probe

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

TaqMan® MGB Probe
Catalog: 4316033
Size: 20,000 pmol

UltraPure™ Agarose

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose

UltraPure™ Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to >20 kb. Features of UltraPure™ Agarose: • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved packaging The new environ...
Catalog: 16500500
Size: 500 g

Gateway® pT-Rex™-DEST30 Vector

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12301-016
Size: 6 µg

pcDNA™4/TO

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

A Tetracycline-Regulated Expression System without Viral Transactivators The T-REx™ System yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2). Specific Activation The T-REx™ System uses a repressor mechanism that blocks transcription from the powerful CMV promoter in the absence of tetracycline. Because the T-REx™ System...
Catalog: V1020-20
Size: 20 µg

pcDNA™5/TO

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

pcDNA™5/TO is a 5.7 kb expression vector designed for use with the T-REx™ System (Catalog nos. K1020-01 and K1020-02) available from Invitrogen. The vector allows tetracycline-regulated expression of the gene of interest in mammalian host cells expressing the Tet repressor (TetR) from the pcDNA™6/TR vector (Catalog no. V1025-20). Details about the pcDNA™5/TO vector can be obtained from the manual that can be downloaded from our website with V1025-20.
Catalog: V1033-20
Size: 20 µg

Tempus™ 12-Port RNA Isolation Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

This Applied Biosystems® product is designed for the purification of RNA from whole blood for real-time RT-PCR, microarrays, or other high-quality RNA experiments. 12 samples can be processed in about 45 minutes. For use with the ABI PRISM® 6100 Nucleic Acid PrepStation, sufficient reagents are included for 24 purifications. • Extract pure, intact RNA • Isolate more than 6 µg of RNA from 3 mL blood • Simple workflow—less time and fewer steps than other kits (see Figure) • Highly stable RNA, free of contaminating gDNA, and inhibitors; no DNase treatment required • Purified RNA used for the gene...
Catalog: 4378672
Size: 24 preps

Invitrogen Magnetic Stand

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers

Magnetic Stand is part of the I-SAGE kit
Catalog: R670-01
Size: each

AcroMetrix® KRAS FFPE Process Controls

Category: Products & Services > Controls, Standards & Ladders > Quality Controls for Clinical & Serology Testing Labs > Clinical Laboratory Controls & Panels

The AcroMetrix® KRAS FFPE Process Controls are intended for use in assessing the performance of the isolation, purification, and detection of nucleic acid associated with specific KRAS mutations. Routine use of the KRAS FFPE Process Controls enables laboratories to monitor day-to-day workflow precision and to identify possible variations such as lot-to-lot performance of assay reagents and operator variation. The AcroMetrix® KRAS FFPE Process Controls can also assist in identifying increases in random or systematic error in testing systems. The AcroMetrix® KRAS FFPE Process Controls have been ...
Catalog: 950450
Size: 8 vials

pCEP4

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

These vectors are designed for high-level, constitutive expression from either the CMV or RSV promoters. Both vectors contain the EBNA-1 gene for episomal expression in primate and canine cell lines.
Catalog: V044-50
Size: 20 µg

Gateway® Vector Conversion System with One Shot® ccdB Survival Cells

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > Vector Conversion Systems

The Gateway® Vector Conversion System is designed to convert any vector into a Gateway® destination vector using restriction endonucleases and ligase. A Gateway® cassette containing att R recombination sites flanking a ccd B gene (1) and a chloramphenicol-resistance gene are blunt-end cloned into the multiple cloning site of any vector. ccdB Survival 2 competent cells allow propagation of the Gateway® vectors containing the ccd B gene. The Gateway® Vector Conversion System offers: • Conversion of any expression vector into a Gateway® destination vector • Three cassettes to construct a destinat...
Catalog: 11828-029
Size: 1 kit

Primer Express® Software v3.0.1, 1-User License

Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software

The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination. • Enables individual oligonucleotide design for real-time PCR applications. • Supports assays based o...
Catalog: 4363991
Size: 1 license

pcDNA™3.1/Hygro (-)†

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

This pcDNA™3.1/Hygro (-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Hygromycin selectable marker and a reverse-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+...
Catalog: V875-20
Size: 20 µg

pcDNA™3.1/Zeo (-)

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

This pcDNA™3.1/Zeo (-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a reverse-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+) or ...
Catalog: V865-20
Size: 20 µg

pREP4

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

These vectors are designed for high-level, constitutive expression from either the CMV or RSV promoters. Both vectors contain the EBNA-1 gene for episomal expression in primate and canine cell lines.
Catalog: V004-50
Size: 20 µg

LIVE/DEAD® Violet Viability/Vitality Kit, for 405 nm excitation

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Vitality Assay Kits

The LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. Calcein violet AM and aqua-fluorescent reactive dye are optimal dyes for this application; both dyes utilize the violet laser allowing other laser lines to be used for conventional fluorochromes.
Catalog: L34958
Size: 1 kit

PureLink® HiPure Plasmid Maxiprep Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Plasmid DNA Purification Kits

The PureLink® HiPure Plasmid Purification Systems are designed to isolate transfection-grade plasmid DNA from E. coli in less than 2 hours. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 ml bacterial culture, at a purity that is comparable to that achieved with two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include: • Labor- and time-saving —No need for extra steps to remove endotoxin and other contaminants • High purity, low endotoxin product —pure enough for mammalian cell transfection • Versatility —p...
Catalog: K2100-07
Size: 25 preps

Geneticin® Selective Antibiotic (G418 Sulfate), 50 mg/ml Solution

Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics

Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a 50 mg / ml solution in...
Catalog: 10131-027
Size: 100 ml

VP-SFM AGT™

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Virus Production Media

VP-SFM AGT™ is a serum free, ultra low protein medium (5 µg/ml) in a new easy-to-use granular format. VP-SFM AGT™ is designed for the growth of VERO cells for virus production. It is also suitable for the growth of COS-7, MDCK, BHK-21 and Hep2 cells and for recombinant protein production.
Catalog: 12559-019
Size: 10 L

1 Kb Plus DNA Ladder

Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > DNA Ladders

1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels. Round, easy to remember ba...
Catalog: 10787-026
Size: 1000 µg

GeneScan™ 500 TAMRA™ Size Standard

Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > Fragment Analysis Size Standards

GeneScan™ 500 TAMRA™ Size Standard is an internal lane size standard developed for use with the Applied Biosystems fluorescence- based DNA electrophoresis systems. The use of an internal lane size standard during electrophoresis enables automated data analysis and precise DNA fragment size comparisons between electrophoresis runs. GeneScan™ 500 TAMRA™ Size Standard is designed for sizing DNA fragments in the 35-500 bp range and provides 16 single-stranded labeled fragments of 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490, and 500 bases. Each of the DNA fragments is lab...
Catalog: 401733
Size: 800 reactions

pcDNA™3.2-DEST

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression • att R sites for Gateway® cloning, enabling recombination with att L-flanked fragments •C-terminal V5 tag for easy detection •Neomycin resistance gene for stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli Gateway® Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination ve...
Catalog: 12489-019
Size: 6 µg

pYES2

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Yeast Expression Vectors

The pYES2 vector is designed for native expression of your protein of interest in S. cerevisia e. It contains the URA3 gene for selection in yeast and 2µ origin for high-copy maintenance.
Catalog: V825-20
Size: 20 µg

pcDNA™6.2-DEST

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-DEST vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression • att R sites for Gateway® cloning, enabling recombination with att L-flanked fragments •C-terminal V5 tag for easy detection •Blasticidin resistance gene for efficient stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli Gateway® Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® d...
Catalog: 12489-027
Size: 6 µg

pTEF1/Zeo

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Yeast Expression Vectors

pTEF1/Zeo is a 3.6 kb vector designed to allow you to create your own cloning and/or expression vectors that confer resistance to Zeocin™ in both E. coli and yeast strains. The vector contains the Sh ble Zeocin™ resistance gene driven by the Saccharomyces cerevisiae TEF1 promoter for selection in any yeast. In addition, the synthetic EM-7 bacterial promoter is present for selection in E. col i. Several unique restriction sites flank the ZeoCassette™ region to allow easy subcloning of the region or cloning of additional elements into pTEF1/Zeo. This vector is also useful for generating gene dis...
Catalog: V503-20
Size: 20 µg

TaqMan® Universal PCR Master Mix, 1-Pack (1 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Applied Biosystems® TaqMan® Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® real-time PCR instru...
Catalog: 4304437
Size: 200 reactions