Products from Pbs - Phosphate-Buffered Saline 10x, Ph 7.4 to Midi-Gel Adapters

PBS - Phosphate-Buffered Saline 10X, pH 7.4

Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS

Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Catalog: AM9624
Size: 500 ml

20X SSC

Category: Products & Services > Buffers & Chemicals > General Buffers > SSC Buffers

Molecular biology grade, Ambion® 20X SSC solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating non...
Catalog: AM9770
Size: 500 ml

StemPro® Accutase® Cell Dissociation Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Other Cell Dissociation Reagents

StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Catalog: A11105-01
Size: 100 ml

Opti-MEM® Reduced Serum Medium, GlutaMAX™

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I

Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 51985-034
Size: 500 ml

Opti-MEM® Reduced Serum Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I

Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 31985-070
Size: 500 ml

Opti-MEM® Reduced Serum Medium, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I

Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 11058-021
Size: 500 ml

Novex® Tris-Glycine Transfer Buffer (25X)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Transfer Buffers

Novex® Tris-Glycine Transfer Buffer (25X) is specially optimized for western blot transfer applications using Tris-Glycine gels.
Catalog: LC3675
Size: 500 ml

Histomount Mounting Solution

Category: Products & Services > Buffers & Chemicals > Microscopy Reagents

This mounting solution is designed for permanent mounting of DAB and BCIP/NBT. Dehydrate tissue before use. This is the same solution that is provided in the Histostain® -SP DAB Kit.
Catalog: 00-8030
Size: 15 ml

Sponge Pad for Blotting (8)

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Blotting, Drying & Staining Accessories

Replacement Parts for Electrophoresis Apparatus
Catalog: EI9052
Size: each

3 M Sodium Acetate, pH 5.5

Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals

Ambion® Molecular biology grade, 3 M Sodium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for cont...
Catalog: AM9740
Size: 100 ml

Buffer Dam for XCell SureLock® and XCell II™

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Other Gel Electrophoresis Accessories

Replacement Parts for Electrophoresis Apparatus
Catalog: EI0012
Size: each

DMEM, High Glucose, no Glutamine, no methionine, no cystine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES • L-methio...
Catalog: 21013-024
Size: 500 ml

MicroAmp® Fast Optical 96-Well Reaction Plate, 0.1 mL

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > PCR Plates

The MicroAmp® Fast Optical 96-Well Reaction Plate is an integral component of the Applied Biosystems® Fast PCR Systems, which reduces PCR reaction time from 2 hours to as little as 25 minutes. • Maximum thermal conductivity for precise thermal cycling. • Reproducible, specific and sensitive PCR results in just 25 minutes. • Validated with other system components for consistent results. Increased Thermal Contact for Faster, More Uniform Heating The advanced design of the MicroAmp™ Fast Optical 96-Well Reaction Plate dramatically increases its thermal contact with the Applied Biosystems® Veriti™...
Catalog: 4346907
Size: 10 plates

0.5 M EDTA, pH 8.0

Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals

Ambion® Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for cont...
Catalog: AM9260G
Size: 100 ml

AmpFLSTR® Identifiler® Plus PCR Amplification Kit User Guide

Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins

This is the User Guide Manual for the AmpFLSTR® Identifiler® Plus PCR Amplification Kit. Note: For Forensic or Paternity Use Only.
Catalog: 4440211
Size: 1 manual

Quantifiler® Duo DNA Quantification Kit User's manual

Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins

This is the user's manual for the Quantifiler® Duo DNA Quantification Kit. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4391294
Size: 1 manual

Quantifiler® Human DNA Quantification Kit

Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Quantification Kits

This real-time PCR-based kit is designed for the quantification of amplifiable human DNA that is present in a sample, and has been validated for human identification applications. An internal PCR control is formulated into each reaction to identify samples that may contain PCR inhibitors. • Quantifies DNA from a wide variety of sources including buccal swabs, blood, semen, tissue, and other human DNA sources making this kit suitable for casework, databasing, and paternity labs. • Chemistry compatible with commonly used extraction technologies for easy integration into current lab workflow. • P...
Catalog: 4343895
Size: 400 reactions

Gel Loading Buffer II (Denaturing PAGE)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue. Supplied in one 1.4 mL tube. All Ambion® Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. These are the same solutions we use in-house and in our kits.
Catalog: AM8546G
Size: 1 ml

MEM, HEPES, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • HEPES • L-glutamine • Phenol Red
Catalog: 12360-038
Size: 500 ml

William's E Medium, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Williams' Media E

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. This media can also be used for growing hepatocyte cells of different species, such as human HepaRG™ cells. This William's E Medium is modified as follows: With Without • Phenol Red • L-glutamine • HEPES
Catalog: 12551-032
Size: 500 ml

Medium 199, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Media 199

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. Life Technologies offers a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This M199 is modified as follows: With Without • L-glutamine • Phenol Red • HEPES
Catalog: 11043-023
Size: 500 ml

YPD Broth, Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Yeast Media

Gibco® YPD Broth is a ready-to-use liquid growth media used for yeast culture. Our filter-sterilized Gibco® YPD Broth can be used for nearly all research applications that require a liquid yeast culturing step such as protein expression, cloning, and expansion. • Gibco® microbiological media • Convenient ready-to-use liquid format • 1 L size available YPD Broth Recipe YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris ( P. pastoris ) and Saccharomyces cerevisae ( S. cerevisae ) yeast strains. Formulation per One Liter: 10 g Yeast extract 20 g Bac...
Catalog: A13745-01
Size: 1 L

Cell Dissociation Buffer, enzyme-free, PBS

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Cell Dissociation Buffers

Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Catalog: 13151-014
Size: 100 ml

DMEM/F-12, HEPES

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM⁄F-12 Media

Dulbecco's Modified Eagle Medium : Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM / F-12 is modified as follows: With • L-glutamine • HEPES • Phenol Red
Catalog: 11330-032
Size: 500 ml

Blotting Roller, 8.6 cm wide

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Blotting, Drying & Staining Accessories

The Blotting Roller is an 8.6 cm wide roller designed for easy and efficient removal of bubbles when assembling gel/membrane sandwiches for blotting both E-PAGE™ Gels and mini-gels such as NuPAGE® and Novex¤ Tris-Glycine Gels. It consists of a Delrin roller attached to a stainless steel handle. The Incubation Tray enables convenient and even staining of E-PAGE™ blots. The tray is 10 cm (l) x 14 cm (w) x 3 cm (d) with a lid. It is suitable for use with immunodetection or blot stains.
Catalog: LC2100
Size: 1 unit

E-PAGE™ Blotting Pads (Reusable), 8.6 cm x 13.5 cm

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Blotting, Drying & Staining Accessories

Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
Catalog: LC2101
Size: 4 pads

RPMI 1640 Medium, no Glucose

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > RPMI Medium 1640

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This RPMI is modified as follows: With Without • L-glutamine • Glucose • Phenol Red • HEPES
Catalog: 11879-020
Size: 500 ml

Advanced DMEM/F-12

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM⁄F-12 Media

Advanced DMEM / F-12 (Dulbecco's Modified Eagle Medium / Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM / F-12, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology. i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat. This Advanced DMEM / F-12 is manufactured as follows: With Without • Glucose • L-glutamine • Non Essential Amino Acids • HEPES • Sodium pyruvate • Phenol Red
Catalog: 12634-010
Size: 500 ml

Advanced RPMI 1640

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > RPMI Medium 1640

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology. i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat. This Advanced RPMI 1640 is manufactured as follows: With Without • Glucose • L-glutamine • Non Essential Amino Acids • HEPES • Sodium pyruvate • Phenol Red
Catalog: 12633-012
Size: 500 ml

Advanced DMEM

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology. i Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero. This Advanced DMEM is manufactured as follows: With Without • High glucose • L-glutamine • Non Essential Amino Acids • Sodium pyruvate • Phenol Red
Catalog: 12491-015
Size: 500 ml

Bottle Weight

Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Other Tubes & Accessories

The new Gibco® bottle has its own bottle weight for keeping order in the water bath! The bottle weight fits the new 500 mL and 1 L Gibco® bottles, keeping them upright in the water to further reduce your risk of contamination. Product Use For Research Use Only. Not for use in diagnostic procedures.
Catalog: A10988-01
Size: 1 each

0.5% Trypsin-EDTA (10X), no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin

Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing This Trypsin is modified as follows: With Without • EDTA • Phenol Red
Catalog: 15400-054
Size: 100 ml

MicroAmp® Cap Installing Tool (Handle)

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > PCR Machine (Thermal Cycler) Accessories

The MicroAmp® Cap Installing Tool helps you install strips of MicroAmp® caps onto MicroAmp® reaction tubes in a tray⁄retainer set or 96-well reaction plate. • Available in handle style • Seal 8 or 12 cap strips • Recessed cup for pressing caps onto tubes • Convenient tool ensures proper sealing Press and Seal Caps The MicroAmp® Cap Installing Tool enables you to ergonomically seal MicroAmp® reaction tubes and plates. This tight seal will prevent well-to-well contamination and sample loss. Used With MicroAmp® Optical 8-Cap Strip, MicroAmp® Optical 8-Tube Strip, 0.2 ml, MicroAmp® Optical 96-Well...
Catalog: 4330015
Size: 1 tool

Goat Serum, New Zealand Origin

Category: Products & Services > Cell Culture & Transfection Reagents > Serum > Serum From Other Blood

Sera Category: Specialty Origin: New Zealand.
Catalog: 16210-064
Size: 100 ml

PureLink® Viral Collection Tubes

Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Other Tubes & Accessories

The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Catalog: 12282-100
Size: 100 tubes

DMEM, Low Glucose, GlutaMAX™, Pyruvate

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • HEPES • Sodium Pyruvate • GlutaMAX™ • Phenol Red
Catalog: 10567-014
Size: 500 ml

NuPAGE® Antioxidant

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Antioxidants

NuPAGE® Antioxidant is a proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis. NuPAGE® Antioxidant is added to the running buffer in the upper (cathode) buffer chamber when performing protein gel electrophoresis under reducing conditions. NuPAGE® Antioxidant is provided in a 15 ml size. NuPAGE® Antioxidant must be used with samples reduced using NuPAGE® Sample Reducing Agent or other reducing agent. NuPAGE® Antioxidant is optimized for the neutral pH of NuPAGE® gels and is not compatible with other gel chemistries. NuPAGE® Antioxidant may also be ad...
Catalog: NP0005
Size: 15 ml

DMEM, High Glucose, no Glutamine, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 31053-028
Size: 500 ml

Ham's F-12K (Kaighn's)

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > F-12 Nutrient Mixture

Ham's F-12K (Kaighn's) medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) medium was developed for primary human hepatocytes, as well as some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) medium is manufactured as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 21127-022
Size: 500 ml

McCoy's 5A (Modified)

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > McCoy's 5A

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. This McCoy's 5A is modified as follows: With Without • High Glucose • Sodium Pyruvate • L-glutamine • HEPES • Bacto-peptone • Phenol Red
Catalog: 16600-082
Size: 500 ml

Trypan Blue Stain 0.4%, for use with the Countess® Automated Cell Counter

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

Trypan blue stain is a well-accepted method for staining dead cells. It can enter into cells with compromised membranes and is the method used with the Countess® automated cell counting platform.
Catalog: T10282
Size: 2 × 1 ml

NuPAGE® Transfer Buffer (20X) (125 ml)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Transfer Buffers

NuPAGE® Transfer Buffer (20X) (125 ml) is a buffer used to transfer proteins from NuPAGE® gels to membranes for western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use. Related Link • Learn more about other Invitrogen products for your protein transfer needs .
Catalog: NP0006
Size: 125 ml

Fungizone® Antimycotic, Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics

Fungizone® antimycotic is a formulation of Amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension. Amphotericin B is used to prevent the growth of fungi by causing an increase in fungal plasma membrane permeability. Gibco® Fungizone® antimycotic is used for the prevention of yeast and multicellular fungi contamination of cell cultures. Gibco® Fungizone contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per ml in distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/ml. Life Technologies offers a variety of antibi...
Catalog: 15290-018
Size: 20 ml

MEM Amino Acids Solution, 50X

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Amino Acid Solutions

Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Catalog: 11130-051
Size: 100 ml

Novex® Tris-Glycine SDS Sample Buffer (2X)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers

Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2676
Size: 20 ml

TrypLE™ Express (1X), Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > TrypLE™

TrypLE™ Express is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. Life Technologies offers a variety of TrypLE ™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Express is: Gentle on cells —maintains cell health for reproducible results Room-temperature stable —convenient to use Easy to use —substitutes directly into existing protocols, with no inactivation required Animal origin fre...
Catalog: 12605-010
Size: 100 ml

MEM Vitamin Solution (100X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Vitamin Solutions

Gibco® MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Vitamin Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001...
Catalog: 11120-052
Size: 100 ml

Penicillin-Streptomycin, Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics

The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibit...
Catalog: 15070-063
Size: 100 ml

ACK Lysing Buffer

Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents

Gibco® ACK (Ammonium-Chloride-Potassium) Lysing Buffer is suitable for use in the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library. cGMP Manufacturing and Quality System Gibco® ACK Lysing Buffer is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
Catalog: A10492-01
Size: 100 ml

Midi-Gel Adapters

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Other Gel Electrophoresis Accessories

The Midi Gel Adapter is designed for use with the Novex® Midi Gel Cassette (NuPAGE® or Tris-Glycine Gels) to perform electrophoresis using a BioRad Criterion™ Cell. The Midi Gel Adapter is also supplied with the Novex® Midi Gels with Adapters Kit.
Catalog: WA0999
Size: 10⁄box