Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Mouse IgM Specificity: Mouse IgM Reactivity: Mouse Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Catalog: 62-6820
Size: 1 ml
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Reactive Oxygen & Nitrogen Species
OxyBURST® Green H 2 DCFDA succinimidyl ester (2',7'-dichlorodihydrofluorescein diacetate, SE) is an amine-reactive assay reagent that can be used to prepare oxidation-sensitive conjugates of a wide variety of biomolecules and particles, including antibodies, antigens, peptides, proteins, dextrans, bacteria, yeast and polystyrene microspheres. Following conjugation to amines, the two acetates of OxyBURST® Green H 2 DCFDA can be removed by treatment with hydroxylamine at neutral pH to yield the dihydrofluorescein conjugate. OxyBURST® Green H 2 DCFDA conjugates are nonfluorescent until they are o...
Catalog: D-2935
Size: 5 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Goat IgG Specificity: Goat IgG Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, Western blotting
Catalog: 61-1620
Size: 1.5 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Mouse IgG2a Specificity: Mouse IgG2a. Exhibits no reactivity with other Mouse IgG subclasses, IgA, IgM, or light chains. Reactivity: Mouse Applications: ELISA, Immunohistochemistry (FFPE), Western blotting
Catalog: 61-0220
Size: 0.45 mg
Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chromogens
TMB (Tetramethyl Benzidine) chromogen solution may be used as a substrate for horseradish peroxidase (HRP) in ELISAs.
Catalog: 00-2023
Size: 500 ml
Category: Products & Services > Labeling & Detection Products > BioConjugates > Lectins
Lectin PNA is specific for terminal β-galactose. It will agglutinate human erythrocytes, but only after neuraminidase treatment.[ALTREF PN40265]
Catalog: L32458
Size: 1 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Neutravidin
Our horseradish peroxidase (HRP) conjugate of NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2664
Size: 1 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Goat whole molecule anti-human immunoglobulin Format/Formulation: HRP Conjugate
Catalog: AHI0304
Size: 0.7 mg⁄ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Human IgG Specificity: Human IgG (H+L) Reactivity: Human IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background....
Catalog: 62-7120
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Mouse IgG (H+L) Specificity: Mouse IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background. Enhanced sensitivity,...
Catalog: 62-6522
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Rabbit IgG Specificity: Rabbit IgG Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Catalog: 65-6120
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Detection Kits & Reagents > Protein A
Protein A from Staphylococcus aureus, Cowan I Strain conjugated with highly purified horseradish peroxidase (RZ > 3.0). The product is liquid containing 10 mM phosphate, 0.15 M NaCl buffer, pH 7.4, 1% ovalbumin, 40% glycerol, and 0.1% proclin as preservative.
Catalog: 10-1023
Size: 3 mg
Category: Products & Services > Buffers & Chemicals > Nucleic Acid Reagents
Yeast tRNA is suitable for use as a carrier in nucleic acid purification and precipitation procedures. It is supplied in lyophilized form.
Catalog: 15401-011
Size: 25 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Sheep IgG (H+L) Specificity: Sheep IgG (H+L) Applications: Flow cytometry, Immunofluorescence ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background. Enhanced sensitivity, higher titer, lower background -- try them t...
Catalog: 81-8611
Size: 1.5 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Isotype Antibody Controls
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Buffer: PBS Preservative: 0.1% Sodium Azide
Catalog: 10400C
Size: 5 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin Inhibitors
Soybean Trypsin Inhibitor is one of several protease inhibitors found in soybeans. Trypsin is inhibited at a molar ratio of 1:1, while chymotrypsin and plasmin are inhibited to a lesser extent. Soybean trypsin inhibitor will also inhibit other serine proteases. Gibco® Soybean Trypsin Inhibitor, powder has an activity greater than 7,000 BAEE units per mg. A 0.5 mg/mL Soybean Trypsin Inhibitor stock solution should be made by dissolving the non-sterile powder in 0.01M Borate Buffer or Dulbecco's Phosphate Buffered Saline (DPBS), without calcium and magnesium. Filter sterilize the stock solution ...
Catalog: 17075-029
Size: 1 g
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Rat IgG (H+L) Specificity: Rat IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Catalog: 62-9520
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Sheep IgG Specificity: Sheep IgG Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Catalog: 61-8620
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Isotype Antibody Controls
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Catalog: 10500C
Size: 5 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Anti-Hapten Antibodies
Immunogen: DNP Specificity: Specific for DNP (2,4-Dinitrophenol). Reactivity: DNP Applications: ELISA
Catalog: 71-3500
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Clone/PAD: ZF24 Immunogen: FITC Specificity: Specific for FITC. Reactivity: FITC Applications: WB, ELISA, Immunohistochemistry, Immunoprecipitation
Catalog: 71-1900
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Modifying Enzymes
DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to RNA-PCR amplification. It is purified and tested for non-detectable levels of RNase contamination. Absence of RNase is tested by performing a ribonuclease assay with RNA ladder. Application Removing DNA from RNA and protein preparations. Specific activity Specific activity is >10,000 units/mg. Source Purified from bovine pancreas Performance and...
Catalog: 18068-015
Size: 100 units
Category: Products & Services > Cell Culture & Transfection Reagents > Serum > Specialty Fetal Bovine Serum
Sera Category: Specialty Origin: United States. Endotoxin level: 50 EU/ml (Specification is checked and recorded; levels routinely Hemoglobin level: 25 mg/dl.
Catalog: 26400-036
Size: 100 ml
Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting.
Catalog: FNN0021
Size: 100 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Catalog: 4318930
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels
DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only protein thiols that are accessible to this water-soluble reagent are modified.
Catalog: D-8451
Size: 10 g
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus ( Taq ) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. Features of this enzyme: AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy Its thermal activity profile makes it ...
Catalog: N8080160
Size: 250 units
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Goat whole molecule anti-human immunoglobulin Format/Formulation: Biotin Conjugate
Catalog: AHI0309
Size: 0.7 mg⁄ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Mouse IgA Specificity: Mouse IgA Reactivity: Mouse Applications: ELISA, Immunohistochemistry (FFPE), WB
Catalog: 62-6720
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Mouse IgG (H+L) Specificity: Mouse IgG (H+L) Applications: Useful as a secondary antibody in many types of immunoassays, including: WB, ELISA, and Immunohistochemistry. ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of bac...
Catalog: 62-6540
Size: 2 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers
NuPAGE® LDS Sample Buffer (4X) (250 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sampl...
Catalog: NP0008
Size: 250 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Simply add template and primers, reducing set-up time by half (see figure). PCR SuperMix contains Mg ++ , dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient for routine PCR of fragments up to 5 kb. PCR SuperMix is supplied at a 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions.
Catalog: 10572-014
Size: 100 reactions
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Immunogen: Chicken/Turkey IgG(H+L) Specificity: Chicken/Turkey IgG(H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, Western blotting
Catalog: 61-3120
Size: 0.75 mg
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories
Sanitization Pack for the PURELAB® flex. The Sanitization Cartridge sanitizes tubing or pipe work to remove bacteria, reduce bio film, and protect the system against bacterial growth. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4474537
Size: 1 pack
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Neuronal Cell Media
GIBCO® Astrocyte Medium has been specially formulated to support the growth of primary human and rat astrocytes while retaining their phenotypes. The medium has been internally validated and optimized to provide an optimal balance between cell growth and the maintenance of cell viability, normal phenotype, and function. If more rapid proliferation is desired, addition of an appropriate amount of epidermal growth factor (EGF) is recommended, although cells may exhibit morphological and phenotypic changes. Astrocytes are a type of glial cells of the nervous system with a typical star shape. It h...
Catalog: A12613-01
Size: 500 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits
The Ambion® MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. MEGAclear™ efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Each kit includes sufficient reagents for 20 reactions. • Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components • High recovery from 1 ng to 500 µg of input • Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections • Quick protocol—purify RNA in le...
Catalog: AM1908M
Size: 20 rxns w⁄ Manual
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
Reactivity: FITC-SA will react with biotin.
Catalog: 43-4311
Size: 1 mg
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
The Vybrant® DiD cell-labeling solution is a dye delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells for use in cell-cell fusion, cellular adhesion and migration applications.
Catalog: V-22887
Size: 1 ml
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solid (D-282), as a paste (N-22880) or as crystals (D-3911).
Catalog: V-22885
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Affinity purified whole antibody goat anti-mouse IgG conjugated to Cy5®. To minimize cross-reactivity, this antibody has been absorbed against human IgG and human serum.
Catalog: A10524
Size: 0.5 ml
Category: Products & Services > Buffers & Chemicals > Denaturing Agents
Molecular biology grade, Ambion® SDS, 10% Solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. SDS is used as a component of both hybridization and wash buffers for northern and Southern blots and has many other uses around the lab.
Catalog: AM9823
Size: 1 L
Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Adhesive Film & Other Plate-Related Accessories
This precision plate holder is for use with Applied Biosystems® 7300 real-time PCR systems (SN 273000878 and above), as well as 7500 real-time PCR systems (SN 275001330 and above). Customers with earlier serial numbers should contact Product Support .
Catalog: 4367032
Size: 1 each
Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chromogens
Stable DAB is a stable, pre-diluted chromogen system designed to clearly localize tissue, DNA, mRNA, and antigens in in situ hybridization and immunoperoxidase procedures. Stable DAB is a ready-to-use reagent containing the correct amounts of diaminobenzidine and hydrogen peroxide. Stable DAB does not fade, creating a permanent record of results under a standard coverslip. It is color coded red for easy user identification.
Catalog: 750118
Size: 250 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Running Buffers
Molecular biology grade, Ambion® 10X TBE solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating...
Catalog: AM9865
Size: 4 bottle
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Proteases & Peptidases
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g. the presence of SDS or urea) • In the presence of metal chelating agents (e.g. EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization. Performance and Quality Testing: End...
Catalog: 4333793
Size: 5 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Mouse monoclonal HP6069; specific for the Fc region of human IgG1. Replaces product number 053300.
Catalog: A-10630
Size: 250 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Mouse monoclonal HP6025; specific for the Fc region of human IgG4. Replaces product number 053800.
Catalog: A-10651
Size: 250 µl
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (NCBI Build 37/hg19) and are required for relative quantitation of copy number targets. TaqMan® Copy Number Assays quantitate the gene of interest and normalize t...
Catalog: 4403326
Size: 750 reactions
Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics
Gibco® Neomycin is produced by Streptomyces fradiae . Polymyxin B Sulfate acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Gibco® Neomycin Sulfate is used for the prevention of bacterial contamination of cell cultures. This antibiotic is highly active against gram-negative bacteria. The recommended working concentration is 50 µg/ml. Life Technologies offers a variety of antibiotics and antimycotics for cell culture applications. Product Use For Research Use Only: Not intended for animal or human diagnosti...
Catalog: 21810-031
Size: 100 g
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Running Buffers
UltraPure™ DNA Typing Grade® 50X TAE Buffer is a sterile-filtered solution of 2 M Tris-acetate and 50 mM EDTA. TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. To prepare a 1X solution, mix one volume of UltraPure™ 50X TAE Buffer with 49 volumes of distilled water. A 1X TAE Buffer solution contains 40 mM Tris-acetate and 1 mM EDTA with a pH of 8.3 at 23“C. Performance and Quality Testing: No DNase activity detected.
Catalog: 24710-030
Size: 1 L