Products from Dihydroethidium (Hydroethidine) to Alexa Fluor® 594 Donkey Anti-Goat Igg (H+L)
The superoxide indicator dihydroethdium , also called hydroethidine, exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red.
Catalog: D-23107
Size: 1 ml
Size: 1 ml
PLUS™ Reagent is used in conjunction with transfection reagents, such as Lipofectamine™, to enhance transfection in adherent cell lines. Some customers find when using Plus™ Reagent that less optimization of DNA concentration is needed to achieve high transfection performance. The addition of Plus™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types (1). PLUS™ Reagent used with Lipofectamine™ Reagent has been demonstrated to improve the transfe...
Catalog: 11514-015
Size: 0.85 ml
Size: 0.85 ml
The amine-reactive PyMPO succinimidyl ester can be used to create bioconjugates with this environment-sensitive fluorophore.
Catalog: S-6110
Size: 5 mg
Size: 5 mg
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: C-6164
Size: 10 mg
Size: 10 mg
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. Life Technologies offers a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This M199 is modified as follows: With Without • Phenol Red • HEPES • L-glutamine
Catalog: 11150-067
Size: 10 × 500 ml
Size: 10 × 500 ml
YEAST HOMOZYGOUS DIPLOID PLATE
Catalog: FL1002
Size: each
Size: each
The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Catalog: S11342
Size: 250 µl
Size: 250 µl
The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.
Catalog: S11363
Size: 250 µl
Size: 250 µl
The phycobiliprotein, allophycocyanin (APC) can be conjugated to proteins such as antibodies and other molecules to produce conjugates with excitation/emission maxima ~650/680 for use in flow cytometry applications. The Protein-Protein Crosslinking Kit (P6305) is optimized to crosslink APC to IgG antibodies.
Catalog: A-803
Size: 0.5 ml
Size: 0.5 ml
The mixed-isomer analog of BODIPY® FL GDP is comprised of the BODIPY® FL fluorophore attached to the 2’ or 3’ position of the ribose ring via an aminoethylcarbamoyl linker. BODIPY® FL exhibits spectral characteristics similar to fluorescein (FITC) or the Alexa Fluor® 488 dye.
Catalog: G-22360
Size: 100 µl
Size: 100 µl
The NativePAGE™ Running Buffer Kit contains the NativePAGE™ Running Buffer (20X), which is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels. The kit also contains the NativePAGE™ Cathode Buffer Additive (20X) that is used with the NativePAGE™ Running Buffer to make the Cathode Running Buffer for the system.
Catalog: BN2007
Size: 1 kit
Size: 1 kit
Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR product is directionally cloned with efficiencies typically >99%. The pDONR™221 vector haa a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
Catalog: 12536-017
Size: 6 µg
Size: 6 µg
AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase that requires thermal activation. Upon thermal activation of the inactive enzyme, the modifier is permanently released and the active enzyme is regenerated. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques. • Specificity —Chemical hot-start enzyme for increased specificity, sensitivity, and yield • Sensitivity —Time-release thermal activation improves sensitivity in low copy number amplifications Hot-Start Kinetics for High Sen...
Catalog: N8080241
Size: 250 units
Size: 250 units
The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of the High Capacity RNA-to-cDNA™ Kit: • Fast —Short reaction time (typically 30–60 min) • Convenient —Easy workflow with few pipetting steps (2 tubes) • Reliable —Reliable reverse transcription of both abundant and limited targets • Flexible —Optimized 2-step protocol enabling multiple PCR reactions from a single reverse transcription reaction Streamline Your cDNA Synthesis ...
Catalog: 4387406
Size: 50 reactions
Size: 50 reactions
Expi293™ Expression Medium is a chemically-defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density cultures of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression. • Supports growth of suspension 293 cultures to densities of over 1.6 x 10 7 cells/mL, with >95% cell viability • Transfection-compatible serum-free suspension culture medium allowing transfection efficiencies of greater than 70% using ExpiFectamine...
Catalog: A1435101
Size: 1000 ml
Size: 1000 ml
GeneScan™ 1000 ROX™ Size Standard is an internal lane size standard developed for use with Applied Biosystems® fluorescence-based DNA electrophoresis systems. The use of an internal lane size standard during electrophoresis enables automated data analysis and precise DNA fragment size comparisons between electrophoresis runs. GeneScan™ 1000 ROX™ Size Standard is designed for sizing DNA fragments in the 47–946 bp range and provides labeled fragments of 47, 51, 55, 82, 85 93 99, 126, 136, 262, 293, 317, 439, 557, 692, 695 and 946 bases. Each of the DNA fragments is labeled with ROX™ fluorophore,...
Catalog: 401098
Size: 400 reactions
Size: 400 reactions
Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 25 reactions. • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time • Amplifies aRNA for gene array analysis and other applications • Efficiently incorporates many modified nucleotides A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® k...
Catalog: AM1333
Size: 25 reactions
Size: 25 reactions
Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 25 reactions. • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time • Amplifies aRNA for gene array analysis and other applications • Efficiently incorporates many modified nucleotides A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® k...
Catalog: AM1333M
Size: 25 rxns w⁄ Manual
Size: 25 rxns w⁄ Manual
SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. • Impermeant to live cells • Excitation/Emission: 504/523 nm • Use with 488 Argon-ion laser • Works with mammalian cells and both Gram-positive and Gram-negative bacteria Simple Viability Determination SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, ...
Catalog: S7020
Size: 250 µl
Size: 250 µl
Ambion® validated GAPDH Positive Control siRNA and non-targeting Negative Control siRNAs with the same chemical modifications for enhanced efficacy as in other Silencer ® Select siRNAs. • Validated siRNA controls for optimizing siRNA experiments • GAPDH Positive Control functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs? Silencer ®Select siRNAs are the next ...
Catalog: 4390843
Size: 5 nmol
Size: 5 nmol
Essential 8™ Medium (published as E8) is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson (published as "E8") and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and is proven to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and has been shown to support iPSC growth for >50 passages without any signs of karyotypic a...
Catalog: A14666SA
Size: 500 ml
Size: 500 ml
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This RPMI is modified as follows: With Without • L-glutamine • Phenol Red • HEPES
Catalog: 11835-055
Size: 10 × 500 ml
Size: 10 × 500 ml
Library Efficiency® DH5α™ Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 10 8 cfu/ µg plasmid DNA. Library Efficiency® DH5α™ Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal Maintains plasmid DNA with minimum recombination events Delivers high yield plasmid preparations for downstream applications M13mp cloning vectors may be propagated on a lawn of DH5α-FT™ , DH5αF...
Catalog: 18263-012
Size: 1 ml
Size: 1 ml
Molecular Probes' proprietary Pro-Q Emerald 300 and Pro-Q Emerald 488 Glycoprotein Stain Kits provide the most advanced technology available for detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. In less than three hours, it is possible to detect as little as 300 pg of glycoprotein per band, depending on the degree of glycosylation, making these stains at least 50-fold more sensitive than standard fuchsin staining.
Catalog: P-20495
Size: 1 kit
Size: 1 kit
Silencer ® Select MALAT1 Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. 5 nmol is provided. • Premium-quality siRNA, highly purified and ready to use • Functionally tested in several common cell lines • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs Silencer ® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, poten...
Catalog: 4455877
Size: 5 nmol
Size: 5 nmol
This highly cross-adsorbed Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L) is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG and human serum.
Catalog: A-11029
Size: 0.5 ml
Size: 0.5 ml
This highly cross-adsorbed Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG and human IgG.
Catalog: A-11034
Size: 0.5 ml
Size: 0.5 ml
This highly cross-adsorbed Alexa Fluor® 594 Goat Anti-Rabbit IgG (H+L) is labeled with our superior red-fluorescent Alexa Fluor 594 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG and human IgG.
Catalog: A-11037
Size: 0.5 ml
Size: 0.5 ml
Clone/PAD: HP6045 Immunogen: Human IgG Specificity: This monoclonal antibody reacts with human IgG heavy chains (Fd region) of all subclasses. Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, Western blotting
Catalog: 05-4500
Size: 0.5 mg
Size: 0.5 mg
Isotype: IgG1K Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Buffer: PBS Preservative: 0.1% Sodium Azide
Catalog: MH1722
Size: 0.5 ml
Size: 0.5 ml
Ambion® Lambda DNA is digested to completion with Hind III. This generates fragments of the sizes 23130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp. These DNA molecular mass markers are supplied with a protocol describing easy labeling of the markers with radioisotopes. They can also be stained with ethidium bromide. One tube containing 200 μg is provided.
Catalog: AM7720
Size: 200 µg
Size: 200 µg
Reactivity: HRP-Avidin reacts with biotin. Applications: Immunohistochemistry, ELISA
Catalog: 43-4423
Size: 5 mg
Size: 5 mg
The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.
Catalog: C10046
Size: 100 µl
Size: 100 µl
Full Product Description Cytokeratin (CK) filaments consist of a complex family of approximately 20 different cytokeratin polypeptides that provide structural integrity to epithelial cells. Our polyclonal pan-CK antibody showed no cross-reactivity to other filament types including vimentin, desmin, glial fibrillary acidic protein and neurofilament protein. This antibody can be used to discern whether a neoplasm originated from an epithelial or non-epithelial source, and also useful for the distinction of metastatic malignant tumors of epithelial origin. Positive tissue: Adenocarcinoma⁄skin⁄squ...
Catalog: 18-0059
Size: 1 ml
Size: 1 ml
F4/80, Rat Anti-Mouse, (Biotin); Biotinylated Monoclonal antibody specific to Mouse F4/80. This antibody is validated for use in Flow Cytometry. Anti-F4/80 recognizes the expressed product of the Emr1 gene.
Catalog: MF48015
Size: 1 ml
Size: 1 ml
Ambion® Silencer ® Firefly Luciferase (GL2 + GL3) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. The control is validated for use in human cell lines and optimized for mouse and rat. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to...
Catalog: AM4629
Size: 5 nmol
Size: 5 nmol
Ambion® Silencer ® GFP (eGFP) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using oth...
Catalog: AM4626
Size: 5 nmol
Size: 5 nmol
Ambion® Silencer ® KIF11 (Eg5) Positive Control siRNA (Human, Mouse, Rat) is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs....
Catalog: AM4639
Size: 5 nmol
Size: 5 nmol
Mouse monoclonal HP6069; specific for the Fc region of human IgG1 and conjugated to the bright, photostable Alexa Fluor 488 dye. Replaces product number 053311.
Catalog: A-10631
Size: 250 µl
Size: 250 µl
The Ambion® Quantu mRNA ™ 18S Internal Standard with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal 18S primers that cannot be extended. By adjusting the ratio of 18S Competimer™s to normal 18S mRNA primers, the signal for 18S mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. Because of its invariant expression across tissues and treatments, 18S ribosomal RNA is an ideal internal control for quantitative RNA analysis. The Universal 18S Inte...
Catalog: AM1718
Size: 100 reactions
Size: 100 reactions
Molecular Probes® cholera toxin conjugates are made from a recombinant version of the B subunit only. This allows us to provide a very high-purity product that is completely free of the toxic A subunit. Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1 , making it a powerful tool for retrograde labeling of neurons. This tracer has been used in a variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall. When used in neuronal tracing applications, CT-B is typically introduc...
Catalog: C-34778
Size: 100 µg
Size: 100 µg
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g. the presence of SDS or urea) • In the presence of metal chelating agents (e.g. EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization. Performance and Quality Testing: End...
Catalog: AM2548
Size: 5 x 1.25 ml
Size: 5 x 1.25 ml
Invitrogen makes blotting easier by providing a variety of pre-cut, pre-assembled membrane/filter-paper sandwiches. A protein's properties ( i.e. , charge, hydrophobicity, etc.) affect its ability to bind to membrane surfaces. Finding the right membrane may require experimenting with your specific protein on different membranes. Store all membranes at room temperature.
Catalog: LC2002
Size: 20⁄pack
Size: 20⁄pack
The Alexa Fluor 350 donkey anti - mouse IgG is labeled with our bright, photostable, blue-fluorescent Alexa Fluor 350 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. The donkey anti - mouse IgG antibodies show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins.
Catalog: A10035
Size: 0.5 ml
Size: 0.5 ml
The Alexa Fluor® 488 Donkey Anti-Goat IgG (H+L) is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from goat. To minimize cross-reactivity, it has been adsorbed against rabbit, rat, mouse, and human IgG.
Catalog: A-11055
Size: 0.5 ml
Size: 0.5 ml
The Alexa Fluor 488 donkey anti—mouse IgG is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. The donkey anti—mouse IgG antibodies show minimum crossreactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep serum proteins.
Catalog: A-21202
Size: 0.5 ml
Size: 0.5 ml
The Alexa Fluor 488 donkey anti—rabbit IgG is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. The donkey anti—rabbit IgG antibodies show minimum crossreactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins.
Catalog: A-21206
Size: 0.5 ml
Size: 0.5 ml
Alexa Fluor® 488 Goat Anti-Rat IgG (H+L) is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rat. To minimize cross-reactivity, it has been adsorbed against mouse IgG, mouse serum and human serum prior to conjugation.
Catalog: A-11006
Size: 0.5 ml
Size: 0.5 ml
The Alexa Fluor 594 chicken anti—mouse IgG is labeled with our bright, red-fluorescent Alexa Fluor 594 dye (which is spectrally similar to Texas Red dye) and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. Chicken antibodies lack a classic "Fc" domain and will not bind to mammalian IgG Fc receptors. To minimize crossreactivity, the antibody has been adsorbed against human and rabbit IgG prior to conjugation.
Catalog: A-21201
Size: 0.5 ml
Size: 0.5 ml
Alexa Fluor® 594 Donkey Anti-Goat IgG (H+L) is labeled with our superior, red-fluorescent Alexa Fluor 594 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from goat. To minimize cross-reactivity, it has been adsorbed against rabbit, rat, mouse, and human IgG.
Catalog: A-11058
Size: 0.5 ml
Size: 0.5 ml
Products from Dihydroethidium (Hydroethidine) to Alexa Fluor® 594 Donkey Anti-Goat Igg (H+L)
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