Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The Alexa Fluor 633 donkey anti—goat IgG is labeled with our bright, far-red—fluorescent Alexa Fluor 633 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from goat. To minimize crossreactivity, it has been adsorbed against rabbit, rat, mouse and human IgG. Fluorescence of this long-wavelength Alexa Fluor dye is not visible to the human eye but is readily detected by most imaging systems.
Catalog: A-21082
Size: 0.5 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Alexa Fluor® 594 Donkey Anti-Goat IgG (H+L) is labeled with our superior, red-fluorescent Alexa Fluor 594 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from goat. To minimize cross-reactivity, it has been adsorbed against rabbit, rat, mouse, and human IgG.
Catalog: A-11058
Size: 0.5 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The Alexa Fluor 488 donkey anti—mouse IgG is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. The donkey anti—mouse IgG antibodies show minimum crossreactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep serum proteins.
Catalog: A-21202
Size: 0.5 ml
Category: Products & Services > Buffers & Chemicals > Nucleic Acid Reagents
Ambion® Carrier RNA (1 mg⁄mL) is a component of all the MagMAX™ Isolation Kits that may be purchased separately. Provided in one tube of 550 μL.
Catalog: 4382878
Size: 500 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The Alexa Fluor 350 donkey anti - mouse IgG is labeled with our bright, photostable, blue-fluorescent Alexa Fluor 350 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. The donkey anti - mouse IgG antibodies show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins.
Catalog: A10035
Size: 0.5 ml
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > RNA Ladders
0.1-2 Kb RNA Ladder is suitable for sizing single-stranded RNA from 0.1 to 2 kb in glyoxal or formaldehyde agarose gels. The ladder consists of ten bands in even increments (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.8, 1.0, 1.5, and 2.0 kb) to simplify size estimation. The 0.3 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-100
Size: 75 µg
Category: Products & Services > Buffers & Chemicals > General Chemicals > Nucleic Acid Precipitation Solutions
Ambion® GlycoBlue™ consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate and is useful as a nucleic acid coprecipitant. Ideal coprecipitant in nuclease protection assays at 1/100 dilution of stock solution, it is provided in five tubes of 0.3 mL each at a concentration of 15 mg/mL. • Ideal for RT-PCR • Increases pellet mass • Quantitative recovery of low concentrations (ng/mL) of nucleic acid • Prevents pellet loss in nuclease protection assays What is a Coprecipitant? Coprecipitants are inert substances used to aid recovery of nucleic acids during alcohol precipi...
Catalog: AM9516
Size: 5 × 300 µl
Category: Products & Services > Instruments, Software, & Accessories > Imaging & Microscopy Accessories > Microscopy Accessories
A suite of accessories has been designed to enable research on living cells using your ArcturusXT™ LCM Instrument. Researchers may use slides or Petri dishes to perform imaging and microdissection experiments. Following a validated protocol from Arcturus® LCM Systems from Applied Biosystems—the leaders in tissue-based microdissection—the live cells may be isolated, remaining viable for subsequent re-culture or direct processing for use in downstream applications. Polyethylene naphthalate (PEN) Membrane Frame Slides for Live Cell Microdissection • Specially optimized for use with live cell expe...
Catalog: LCM0531
Size: 25 each
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
DNAzol® BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes. • Specifically formulated for extraction of genomic DNA from whole blood • Optimized for the rapid Isolation of genomic DNA Efficient Isolation of Genomic DNA from Whole Blood DNAzol® BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol® BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. ...
Catalog: 10974-020
Size: 100 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
The pcDNA™6 BioEase™ Gateway® Biotinylation System provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pcDNA™6/BioEase™-DEST vector (Figure 1) incorporates a 72 amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. This K. pneumoniae tag is recognized and efficiently biotinylated in vivo by a native mammalian system similar to the enzyme Biotin Protein Ligase (BPL) in E. coli (1). Proteins produced from the pcDNA™6/BioEase™-DEST vector are expressed as fusion proteins to this sequen...
Catalog: SA100-02
Size: 50 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
Streptavidin-Horseradish Peroxidase (HRP) conjugate
Catalog: SA100-01
Size: 50 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
Alexa Fluor® 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western ...
Catalog: S-21374
Size: 1 mg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Gateway® Entry Vectors - pENTR
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in...
Catalog: A10463
Size: 10 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Gateway® Entry Vectors - pENTR
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in...
Catalog: A10464
Size: 10 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Gateway® Entry Vectors - pENTR
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryoti...
Catalog: A10462
Size: 10 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Gateway® Entry Vectors - pENTR
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryotic systems • Ribosome bindi...
Catalog: A10465
Size: 10 µg
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 11960-069
Size: 10 × 500 ml
Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Media & Standards
Glutathione agarose prepared by linking the sulfur atom of glutathione to crosslinked beaded agarose can be used for purification of GST fusion proteins. Each milliliter of gel can bind approximately 5-6 mg of boivine-liver GST. Adding excess free glutathione liberates the GST fragment from the matrix which can then be regenerated by washing with a high-salt buffer.
Catalog: G2879
Size: 10 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin
Gibco® Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco® trypsin solutions and recommended dissociation conditions . Life Technologies offers a variety of trypsin formulations and animal origin-free TrypLE™ reagent that feature: Quality Testing Documented Tracea...
Catalog: 25300-120
Size: 20 x 100 ml
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores
Fura-FF pentapotassium salt is a fura-2 derivative that has Ca 2+ sensitivity similar to mag-fura-2, but is relatively insensitive to Mg 2+ . This water-soluble salt form is useful for intracellular loading by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: F14180
Size: 500 µg
Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Northern Blotting Kits & Reagents
An improved formula for preparing denaturing formaldehyde/MOPS agarose gels. Formaldehyde is included. Gels made with this buffer are considerably less brittle than conventional formaldehyde gels, making handling of the gels much easier. One bottle containing 250 mL is provided. NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.
Catalog: AM8676
Size: 250 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Anti-Hapten Antibodies
Clone/PAD: Z021 Immunogen: Synthetic biotin Specificity: Binds strongly to biotinylated proteins. Reactivity: Biotinylated proteins Applications: ELISA, Cell and tissue staining
Catalog: 03-3720
Size: 1 ml
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > Bis-Tris Gels
The NativePAGE™ Novex® Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analysis of native membrane protein complexes, native soluble proteins, molecular mass estimations, and assessment of purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis technique (BN PAGE) developed by Schagger and von Jagow (1-3). With the NativePAGE™ Novex® Bis-Tris Gel System, youll: • Simplify resolution of large proteins (15-10,000 kDa) • Analyze membrane-protein complexes in their native conformations • ...
Catalog: BN1004BOX
Size: 1 box
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • HEPES • L-Glutamine • Sodium Pyruvate • Phenol Red
Catalog: 11885-092
Size: 10 × 500 ml
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Metabolic Assay Kits
2-NBDG is a fluorescent glucose analog that has been used to monitor glucose uptake in live cells, as an indicator of cell viability. Although sensitive to its environment NBD fluorescence typically displays excitation/emission maxima of ~465/540 nm and can be visualized using optical filters designed for fluorescein.
Catalog: N13195
Size: 5 mg
Category: Products & Services > Buffers & Chemicals > General Buffers > Other General Buffers
The Ambion® Buffer Kit provides high quality, RNase free buffers for critical RNA analysis procedures. The kit consists of one 100 mL bottle of each of the following solutions: 1 M Tris pH 7.0, 1 M Tris pH 8.0, 5 M NaCl, 1 M MgCl 2 , 0.5 M EDTA pH 8.0, 2 M KCl, 5 M Ammonium Acetate, and DEPC-treated H 2 O. Ammonium Acetate is sterile filtered. All other solutions are autoclaved post-packaging to ensure sterility. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
Catalog: AM9010
Size: 1 set
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
Our streptavidin, Marina Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ~365/460 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.
Catalog: S-11221
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ambion® Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is essential. Six tubes of 25 mg are provided, at a concentration of 40–50 mg/mL. The acetylation process inactivates any trace nuclease activity that might be present. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is routinely added to restriction digests. The molecular weight is ~66 kD. Acetylated BSA is rigorously tested for contaminating nonspecific endonuclease, exonucleas...
Catalog: AM2614
Size: 6 tubes
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD45, clone HI30, PerCP-Cy ® 5.5 conjugate is a mouse monoclonal antibody (mAb) that recognizes human CD45 molecule. The PerCP-Cy ® 5.5 tandem conjugate combines the peridinin-chlorophyll proteins (PerCP) complexes, found in nature, with the Cy ® 5.5 cyanine dye; thereby permitting simultaneous multicolor labeling and detection of multiple targets by utilizing a single excitation source - the 488 nm line of an argon-ion laser. Antibody Specifications Description: CD45, Mouse IgG 1 monoclonal Label: PerCP-Cy ® 5.5 tandem dye Validated Application: Flow Cytometry Species Reactivity: Human Ex...
Catalog: A14796
Size: 25 µg
Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics
Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus . Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco® Hygromycin B is used as a bacterial selective antibiotic in the concentration range of 100 - 1000 µg / ml. This product is supplied as a 50 mg / ml solution. Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene ( hyg or hph ). Hygromycin B uses a different mode of action than Geneticin®, Blasticidin S, or Zeocin™, so it is excellent for use in dual-selec...
Catalog: 10687-010
Size: 20 ml
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
DASPMI (4-Di-1-ASP) is a styryl dye stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane potential.
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
DiOC 6 (3) is a cell-permeant, green-fluorescent, lipophilic dye that is selective for the mitochondria of live cells, when used at low concentrations. At higher concentrations, the dye may be used to stain other internal membranes, such as the endoplasmic reticulum.
Catalog: D-273
Size: 100 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Affinity purified whole antibody goat anti-mouse IgG conjugated to 4% beaded, crosslinked agarose (Sepharose® CL-4B). To minimize cross-reactivity, this antibody has been absorbed against human IgG and human serum.
Catalog: A10516
Size: 2 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
TRIzol® Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour. Key Features of TRIzol® Reagent: • Isolate RNA, DNA, and protein from the same sample • Superior lysis capability, even with difficult sample types • Optimized formulations and pro...
Catalog: 15596-026
Size: 100 ml
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > RNA Ladders
0.5-10 Kb RNA Ladder is suitable for sizing single-stranded RNA ranging from 0.5 kb to 10 kb in glyoxal or formaldehyde agarose gels. The ladder consists of nine bands in even increments (0.5, 1, 1.5, 2, 3, 4, 6, 8, and 10 kb) to simplify size estimation. The 1.5 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-200
Size: 75 µg
Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Catalog: HYL-250
Size: 500 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The Alexa Fluor 405 goat anti-rabbit IgG is labeled with our bright, photostable, blue-fluorescent Alexa Fluor 405 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit.
Catalog: A-31556
Size: 0.5 ml
Category: Products & Services > Labeling & Detection Products > BioConjugates > Protein Conjugates
Actin is an important tool for investigating cytoskeleton dynamics in-vivo . This highly purified actin from rabbit muscle is subjected to depolymerization and subsequent polymerization to ensure that the actin is able to assemble properly. The actin that polymerizes is then separated from remaining monomeric actin by centrifugation, depolymerized and packaged in monomeric form.
Catalog: A12375
Size: 1 mg
Category: Products & Services > Controls, Standards & Ladders > Protein Standards & Ladders > Prestained Protein Standards
The BenchMark™ Pre-Stained Protein Ladder is a standard used to monitor electrophoresis or electrophoretic transfer from gel to membrane in Western blots. It consists of 10 proteins ranging in apparent molecular weight from 6 to 180 kDa. It is designed for use with Tris-Glycine gels. The BenchMark™ Pre-Stained Protein Ladder offers: Proteins covalently bound to a blue dye for easy visualization One protein band coupled to a pink dye for easy orientation and protein identification Affinity-purified proteins to provide sharp bands and clear background Easy-to-use format for direct application to...
Catalog: 10748-010
Size: 2 × 250 µl
Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > Nuclease Control Reagents
Ambion® RNA secure ™ (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution. 10 mL supplied. • No post-treatment autoclaving • Safe, easy-to-use reagent inactivates RNases in solutions • Inactivation process can be repeated to protect against newly introduced contaminants • Compatible with downstream procedures, such as RT-PCR and in vitro transcription • Inactivate RNases in Tris and other solutions that cannot be treated with DEPC RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency ...
Catalog: AM7006
Size: 10 ml
Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Blocks
• The Heat Equalizing Block is an aluminum conduction plate for use with the SSP UniTray and other laboratory procedures that require even heat distribution during thermal cycling. The Heat Equalizing Block is set on top of the SSP UniTray while thermal cycling to ensure even heat distribution • The Membrane Comb Loading Tray is for convenient benchtop loading of 64-lane membrane combs. Polycarbonate tray with two rows of 64 wells, each 2 mm wide • HLA Beyond Tears - By Glenn E. Rodey, Second Edition (De Novo, Inc., Durango, CO 2000) Serves as an introductory text for individuals who are inter...
Catalog: 90000-1D
Size: 1 each
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
Alexa Fluor 488 rabbit anti—rat IgG is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rat. To minimize crossreactivity, it has been adsorbed against human IgG prior to conjugation.
Catalog: A-21210
Size: 0.5 ml
Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals
Ambion® Water Saturated Phenol is supplied premixed and at pH 6.6 (pH 7.9 ± 0.2, with included buffer). Water Saturated Phenol is used in RNA and DNA extraction procedures and is provided in one bottle of 400 mL. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, and easier alternative to preparing solutions from crystalline phenol.
Catalog: AM9712
Size: 400 ml
Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals
Acid Phenol:Chloroform:IAA (125:24:1) is premixed and supplied at pH 4.5 ± 0.2. Provided in one bottle of 400 mL. In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality-tested, saturated phenols are ready to use, eliminating handling problems and offering a more convenient, safer, an...
Catalog: AM9722
Size: 400 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits
The RediPlate™ 96 RiboGreen® RNA Quantitation Kit is a rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assay. The microplate has been pre-loaded with RiboGreen® Reagent, which shows bright green fluorescence upon binding to RNA or DNA (1). You simply add buffer and samples to the microplate wells, wait 10 minutes, and then read the fluorescence in any standard fluorescence-based microplate reader. The versatile RediPlate™ 96 design is ideal for either high-throughput quantitation or the measurement of a small number of samples. The RediPlate™ 96 RiboGreen® RNA Quantitat...
Catalog: R32700
Size: 1 kit
Category: Products & Services > Buffers & Chemicals > General Chemicals > Inducing Agents
Ambion® IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage. IPTG is also used to induce the expression of cloned genes that are under control of the lac operon. Supplied in one bottle containing 5 g.
Catalog: AM9464
Size: 5 g
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...
Catalog: F-14200
Size: 500 µg
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Phagocytosis Kits & Phagocytic Particles
The Molecular Probes® BioParticles® product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles® products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan ( S. cerevisiae ) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dy...
Catalog: E-2864
Size: 10 mg
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qRT-PCR Kits
Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-C T ™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes: • AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA • An additive that red...
Catalog: 4391178
Size: 40 reactions
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The Alexa Fluor® 488 goat anti-mouse IgG 1 is labeled with our exceptionally bright, photostable Alexa Fluor® 488 dye (which is spectrally similar to fluorescein) and is prepared from affinity-purified antibodies that react with the Fc portion of the heavy chain of mouse IgG 1 . To minimize crossreactivity, the antibody has been adsorbed against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG 2a , IgG 2b and IgG 3 prior to labeling.
Catalog: A-21121
Size: 250 µl