Products from Bigdye® Terminator V3.1 Cycle Sequencing Kit to Ion Xpress™ Barcode Adapters 1-16 Kit

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337458
Size: 25000 reactions

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337457
Size: 5000 reactions

Covaris® M220 Focused-ultrasonicator™

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing

The Covaris® M220 Focused-ultrasonicator™ is designed for next generation sequencing applications that require high-quality DNA fragmentation for library preparation. The Covaris® M220 efficiently focuses the minimum amount of energy required directly into a sample, enabling precise control of the hydrodynamic shearing forces that fragment nucleic acids to the selected size. The process is isothermal, so fragmentation is unbiased, the sample is undamaged, and yields are high. The Covaris® focused-ultrasonic technology is considered a DNA shearing standard in many labs worldwide. The Covaris® M...
Catalog: 4482277
Size: 1 instrument

7900HT TaqMan® Array Upgrade

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Real-Time PCR Instrument Accessories

The 7900HT TaqMan® Array Upgrade enables you to run the TaqMan® Arrays – a microfluidic card – on your Applied Biosystems 7900HT Fast Real-Time PCR System. This upgrade gives you access to an efficient, easy-to-use tool that eliminates labor-intensive pipetting steps and enables high throughput gene expression results using the gold-standard 5' nuclease TaqMan® Assay technology. • Convenience – includes everything you need to run the 7900HT TaqMan® Array on your 7900HT system • Success – highly trained service engineers will install and test your 7900HT TaqMan® Array Upgrade to get you up and ...
Catalog: 4329012
Size: 1 kit

Pippin Prep™ System (includes instrument and monitor)

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing

The Pippin Prep™ System is a small tabletop instrument that automates the size selection and collection of DNA for PGM™ sequencer libraries. Simply enter the desired base-pair target range via the software interface and after a 1.5 hr run the system will yield the programmed DNA fractions with virtually no low molecular weight contamination. The tight fragment size distributions obtained provide a more uniform template for amplification. And the processing of samples is simplified through the use of disposable pre-cast agarose gel cassettes. Benefits of the Pippin Prep™ System • Time savings c...
Catalog: 4471271
Size: 1 each

GeneAmp® PCR System 9700 Dual 96-Well

Category: Products & Services > Instruments, Software, & Accessories > Instruments > PCR Machines (Thermal Cyclers)

The Applied Biosystems® GeneAmp® PCR System 9700 is specifically designed for reliable PCR. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The Dual 96-Well GeneAmp® PCR System 9700 is designed for use with 0.2 mL reaction tubes or 96-well reaction plates for high-throughput PCR, cloning, and cycle sequencing applications. • Request a Demo Key Highlights: • Compact design maximizes bench space • Two 96-well sample blocks for high-throughput applications • Interchangeable sample block modules for greater flexibility • Graphical user i...
Catalog: 4343176
Size: 1 instrument

Bioruptor® UCD-200 with accessories for 12 x 0.5 ml Tubes & BioBox-200

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories

The Bioruptor® UCD-200 is a benchtop sonication device used to uniformly process up to 12 x 0.5 ml samples simultaneously in sealed tubes without aerosol formation. Consisting of a high-power ultrasound generating element located below a water bath, the Bioruptor® sonicates at a 20 kHz frequency, similar to a probe sonicator. The Bioruptor® comes with standard 0.5 ml accessories and the BioBox-200 for soundproofing. The Bioruptor® UCD-200 Provides: • Parallel processing of up to 12 DNA samples • Robust and reproducible results • Automated sample prep Get Reproducible DNA Shearing Easily and Au...
Catalog: 4465622
Size: 1 each

Custom siRNA

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

Ambion® Custom In Vivo Ready synthesized siRNA, 10 µmol. These siRNAs are subjected to that extra level of purification and testing required for the introduction of high quality siRNAs into animals.
Catalog: AM16233
Size: 10 µmol

Tango™ CCR5- bla U2OS Cells

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > GPCR Cell Based Assay Kits

For Academic pricing please login or contact us at discoverysciences@invitrogen.com. The Tango™ CCR5- bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 5 (CCR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR- bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase ( bla ) reporter gene under the control of a UAS response element. The Tango™ CCR5- bla U2OS cells are functionally validated for Z' and EC 50 concentrations of MIP-1a. In addition,...
Catalog: K1788
Size: 8 × 10^6 cells

CellSensor® HSE- bla HeLa Cell Line

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits

Activation of the heat shock response⁄unfolded protein response (HSR⁄UPR) occurs in response to a diversity of chemical, environmental, and physiological stress conditions. Transcriptional regulation of the human HSR is mediated by a family of three heat shock transcription factors (HSFs), HSF-1, -2, and -4. Stress-induced activation of quiescent HSF monomers results in their trimerization and accumulation in the nucleus, wherein they bind to and upregulate transcription of target genes (e.g. molecular chaperones, certain proteases, and other stress response genes) harboring a heat shock eleme...
Catalog: K1813
Size: 1 vial

CellSensor® NFkB- bla RAW 264.7 Cell Line

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits

The CellSensor® NFκB-bla RAW264.7 mouse macrophage cell line contains a beta-lactamase reporter gene under control of a stably integrated NFκB response element. This cell line is a clonal population isolated in response to lipopolysaccharide by flow cytometry. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, and has been validated for Zfi-factor and EC50 concentrations of the TLR7 ligand imiquimod. Additional data using alternate stimuli are shown. The molecules responsible f...
Catalog: K1673
Size: 1 vial

CellSensor® Myc- bla HCT116 Cell Line

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits

The CellSensor™Myc- bla HCT116 cell line contains a beta-lactamase reporter gene under the control of Myc binding sequences. The construct was transduced into HCT116 cells using a lentiviral system. HCT116 is a colon cancer cell line which expresses a mutated form of beta-catenin. This form of beta-catenin leads to the accumulation of beta-catenin and constitutive activation of downstream genes such as Myc . This cell line is a clonal population isolated by flow cytometry. It has been validated for cell plating density and DMSO tolerance. The signaling pathway has been validated using RNAi aga...
Catalog: K1467
Size: 1 vial

CellSensor® irf1- bla Ba/F3 Cell Line

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits

Jak/Stat signaling pathways play essential roles in the cellular responses to distinct cytokines. One of the Jak/Stat pathways, Jak2/Stat5, mediates cell proliferation in response to Interleukin - 3 (IL - 3), prolactin, erythropoietin (Epo), and granulocyte - macrophage colony stimulating factor (GM - CSF). JAK2 gene knockout causes embryonic lethality due to defective erythropoiesis, suggesting that the Jak2/Stat5 pathway plays an important role in red blood cell formation. The recent discovery of an activating mutation in JAK2 (V617F) present in a high percentage of myeloproliferative diseas...
Catalog: K1654
Size: 1 vial

CellSensor® Gli- bla NIH3T3 Cell Line

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits

The CellSensor® Gli-bla NIH 3T3 Cell Line contains a beta-lactamase reporter gene under control of the Gli response element stably integrated into NIH 3T3 cells. This cell line is a clonal population isolated by flow cytometry in response to the mouse sonic hedgehog (mShh) ligand. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, as well as validated for Z'-factor and EC50 concentrations of Shh. Additional data using known inhibitors or activators of the pathway have also been...
Catalog: K1642
Size: 1 vial

CellSensor® LEF/TCF- bla SW480 Cell Line

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits

The CellSensor® LEF/TCF-bla SW480 cell line contains a beta-lactamase reporter gene under control of the lymphoid enhancer factor/T cell factor (LEF/TCF) consensus binding sequence stably integrated into SW480 cells, which express a truncated form of the tumor suppressor protein adenomatous polyposis coli (APC). This cell line is a clonal population isolated by flow cytometry, with the pathway validated using RNAi against beta-catenin. The truncated, loss-of-function version of APC expressed in this cell line prevents the proper assembly of the beta-catenin destruction complex. This results in...
Catalog: K1675
Size: 1 vial

CellSensor® LEF/TCF- bla FreeStyle™ 293F Cell Line

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits

Wnt signaling via beta - catenin plays a central role in development and homeostasis. This pathway is invariably disrupted in colorectal tumors and commonly affected by mutation in other cancers. Binding of the Wnt protein to the Frizzled transmembrane receptors initiates a signaling cascade that activates the Dishevelled protein, which then inhibits the serine/threonine kinase Glycogen Synthase - 3 Beta (GSK - 3B). This signal leads to functional inactivation and dissociation of a multiprotein beta - catenin destruction complex made up of the tumor suppressor protein Adenomatous Polyposis Col...
Catalog: K1677
Size: 1 vial

BigDye® Terminator v1.1 & v3.1 5X Sequencing Buffer

Category: Products & Services > Buffers & Chemicals > Sequencing Buffers & Reagents

This sequencing buffer is optimized for use with BigDye® Terminator v1.1 and v3.1 Cycle Sequencing Kits. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4336701
Size: 233 ml

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337456
Size: 1000 reactions

GripTite™ 293 MSR Cell Line

Category: Products & Services > Eukaryotic Cells > Cell Lines > Mammalian Cell Lines

The GripTite™ 293 MSR Cell Line is a genetically engineered Human Embryonic Kidney (HEK 293) cell line that expresses the human macrophage scavenger receptor and strongly adheres to standard tissue culture plates for dependable results. Developed from a 293-H subclone, GripTite™ 293 MSR cells show the same fast cell growth, high transfection efficiency, and high-level expression characteristics of the parental 293-H cells. Unlike most 293 cells, GripTite® 293 MSR cells dont wash away during the repeated manipulations of routine tissue culture or plate washing protocols (Figure 1). Their superi...
Catalog: R795-07
Size: 1 kit

GeneAmp® PCR System 9700 60-Well and 0.5 ml Tube

Category: Products & Services > Instruments, Software, & Accessories > Instruments > PCR Machines (Thermal Cyclers)

The Applied Biosystems® GeneAmp® PCR System 9700 is specifically designed for reliable PCR. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The 0.5 mL GeneAmp® PCR System 9700 is designed for use with 0.5 mL reaction tubes for all of your large volume PCR applications. • Request a Demo Key Highlights: • Heated lid for oil-free operation in a 60-well, 0.5 mL format • Interchangeable sample blocks for increased flexibility • Graphical user interface for easy, intuitive programming • Designed for fast thermal response and exceptional un...
Catalog: 4310899
Size: 1 instrument

Partek® Genomics Suite™ Software (Government)

Category: Products & Services > Instruments, Software, & Accessories > Software > Sequencing & Genetic Analysis Software

Partek® leverages a 17 year commercial history—founded on sound statistical analysis and expanded from a decade of microarray analysis--to today's semiconductor-based sequencing. Partek® Genomics Suite™ software is a full-featured software package for the analysis, comparison, and integration of all your genomic data. This product, which can be ordered through the Life Technologies website, is supplied directly by Partek Incorporated. Partek® Genomics Suite™ Software Gives You: • Sophisticated capabilities for DNA-seq, ChIP-seq, and RNA-seq experiments • Statistical analysis of each base pair ...
Catalog: 4462922G
Size: 1 each

Platinum® Taq DNA Polymerase High Fidelity

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits

Platinum® Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments where high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´a5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase (Table 1). PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. With Platinum® Taq High Fidelity you'll get: Greater than six times higher fidelity than Taq DNA poly-merase Amplification of fragments up to 15 kb (Figure 1) Room temperature reaction...
Catalog: 11304-102
Size: 5000 reactions

FreeStyle™ MAX Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

The FreeStyle™ MAX System is a breakthrough technology for rapid and high-yield mammalian protein production. This new mammalian expression technology is available in two convenient formats. FreeStyle™ MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle™ cells with high efficiency. The FreeStyle™ MAX Expression System combines GIBCO® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293 cells. The only equipment required to use the FreeStyle™ MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinne...
Catalog: 16447-500
Size: 15 ml

E-Gel® Imager Camera Hood Kit

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Imagers & Digital Electrophoresis Systems

The E-Gel® Camera Hood Kit, when combined with a base, forms the E-Gel® Imager System, which is a personal imaging system for documenting and analyzing agarose gels and E-Gel® cassettes. Each E-Gel® Camera Hood Kit includes a compact digital camera mounted in a sleek hood, a universal filter, power and USB cables, and two powerful software applications. There are three bases available for purchase that will work with the E-Gel® Camera Hood Kit: a UV Light Base, a Blue Light Base, and an E-Gel® Adaptor Base. In any of these three configurations, the E-Gel® Imager system provides a small and lig...
Catalog: 4466601
Size: 1 kit

Avadis® NGS Software (Academic)

Category: Products & Services > Instruments, Software, & Accessories > Software > Sequencing & Genetic Analysis Software

Avadis® NGS is a desktop software platform for analysis, visualization, and management of data generated by Next-Generation Sequencing (NGS) platforms. It supports workflows for Alignment, DNA-Seq, RNA-Seq, Small RNA-Seq and ChIP-Seq analysis and is designed with the biologist in mind. Alignment Avadis NGS supports an inbuilt alignment algorithm called COBWeb that supports the alignment of both short and long reads with an arbitrary number of gaps and mismatches. It also includes support for paired end reads and a variety of trimming options and screening databases. DNA-Seq The DNA-Seq workflo...
Catalog: 4467869
Size: 1 software

TaqMan® OpenArray® Genotyping Plate, Custom Format 192 QuantStudio™ 12K Flex

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

TaqMan® OpenArray® Genotyping Plate, Custom Format 192 QuantStudio™ 12K Flex
Catalog: 4471117
Size: 10 pack

TaqMan® PCR Core Reagent Kit (10-Pack)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Perform up to 2,000 PCR reactions with Applied Biosystems® TaqMan® PCR Core Reagent kit, 10-Pack. Applied Biosystems® TaqMan® PCR Core Reagents, 10-pack provides the individual components needed to perform up to 2,000 PCR reactions (50 µl each). Features of the TaqMan® PCR Core Reagent Kit: • Separate components, packaged in 10 tubes, provide maximum flexibility in assay setup • AmpliTaq Gold® DNA polymerase, a chemically modified form of AmpliTaq® DNA Polymerase, improves specificity and yield • AmpErase® UNG protects against subsequent re-amplifications from PCR products containing dU to min...
Catalog: 402930
Size: 2000 reactions

TaqMan® Reverse Transcription Reagents (10-Pack)

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

This Applied Biosystems® kit provides the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, and oligo (dT) 16 , reverse primers are included for cDNA synthesis flexibility. Supplied as 2000 reactions in 10 tubes (200 reactions/tube). • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions TaqMan® Reverse Transcription Reagents provide all...
Catalog: 4304134
Size: 2000 reactions

EdU (5-ethynyl-2’-deoxyuridine)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > BrdU, EdU & EU Reagents

EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a s...
Catalog: E10415
Size: 5 g

Ion 318™ Chip Kit (8 pack)

Category: Products & Services > Slide & Chip Arrays > Semiconductor Chips

Ion 318™ Chip Kits contain chips for sequencing runs using the Personal Genome Machine™ System, the first PostLight™ semiconductor-based sequencer. The Ion 318™ Chip detects polymerase-driven base incorporation and translates this information into digital form. By eliminating the use of light-producing chemistry and optical systems, this revolutionary advance in next-generation sequencing technology makes massively parallel sequencing affordable to nearly every lab. Ion 318™ Chips provide: • Easy handling and loading of templated Ion Sphere™ particles • Over 11 million wells per chip • Compati...
Catalog: 4469497
Size: 8 pack

GeneAmp® dNTP Blend 100 mM (Twelve Paq)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > dNTPs

GeneAmp® dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. GeneAmp® dNTPs with dUTP can be used to generate dU-containing PCR products in a carry-over prevention strategy in conjunction with AmpErase ® Uracil N-glycosylase (UNG). dNTPs have high purity, making them ideal for use in PCR reactions or other applications. dNTPs with dUTP are optimized for use in carry-over prevention. Guaranteed for PCR performance when used with GeneAmp® PCR core reagents and GeneAmp® instrument systems. Premixed dNTP blends are available in various conce...
Catalog: 4303443
Size: 12 x 1 ml

Illumina® TotalPrep™-96 RNA Amplification Kit

Category: Products & Services > Kits & Assays > RNA Amplification Kits

The Ambion® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The kit includes sufficient reagents for 96 reactions. • Perform amplification from just 50 ng of input RNA • Developed for expression profiling using the Sentrix® BeadChips • Bio-16-UTP now included in optimized NTP mix, reducing setup time and maximizing labeling The TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous TotalPrep™ Kit. The IVT...
Catalog: 4393543
Size: 96 reactions

TaqMan® Universal PCR Master Mix, No AmpErase® UNG, 1 Bulk Pack (1 x 50 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4326614
Size: 2000 reactions

MeltDoctor™ HRM Master Mix, 50 mL

Category: Products & Services > Kits & Assays > Genotyping Kits > High Resolution Melt Kits

Applied Biosystems® MeltDoctor™ HRM Master Mix contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM applications. • Low background fluorescence • High brightness in the presence of double-stranded DNA • Minimal temperature shif...
Catalog: 4409535
Size: 50 ml

Centri-Sep™ 96-Well Plates

Category: Products & Services > Columns, Resins & Plates > Spin Filters, Columns, & Purification Plates

Centri-Sep™ 96's hydrated matrix enables effective and reliable high-throughput removal of excess BigDye® terminators from DNA sequencing reactions, giving you clean sequencing reads with uniform signal intensity starting with the first base. The plate's 96-well format enables you to purify 96 samples simultaneously, either manually or by robotics, within 15 minutes. • Processed faster than ultrafiltration columns • Rapid and efficient separations • Buffer is not preselected • Convenient 20-100 µL sample size • Dry gel format • Room Temperature Storage • Microcentrifuge format Designed for Per...
Catalog: 4367821
Size: 50 plates

DNASTAR® Lasergene Genomics Suite Software (Commercial)

Category: Products & Services > Instruments, Software, & Accessories > Software > Sequencing & Genetic Analysis Software

The DNASTAR® Lasergene® Genomics Suite provides all of the software you need for next-generation sequence assembly and analysis in a single, integrated package. Our software uses a proprietary algorithm to quickly and accurately assemble a genome of any size using a reference on a desktop computer, and includes a comprehensive suite of post-assembly analysis tools, including SNP detection and analysis, evaluation of coverage, consensus annotation, and more. With DNASTAR® Lasergene® Genomics Suite Software You Get: • SeqMan® NGen® software for next-generation sequence assembly, Lasergene® Core ...
Catalog: 4462914C
Size: 1 each

TaqMan® Gene Expression Master Mix, 10-Pack (10 x 5 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

Get precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems® TaqMan® Gene Expression Master Mix. TaqMan® Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan® Gene Expression Master Mix has been validated with TaqMan® Gene Express...
Catalog: 4369542
Size: 2000 reactions

MicroAmp® Optical 384-Well Reaction Plate with Barcode

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > PCR Plates

The Applied Biosystems® MicroAmp® Optical 384-Well Reaction Plate with barcode is designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a 384-well format, is designed to work with Applied Biosystems® 384-well Real-Time PCR Systems and Thermal Cyclers. • Frosted to minimize interfering fluorescence from wells of cycling block • Screened to eliminate auto-fluorescent plates • Unique design provides a barrier to ambient air to help insure well-to-well temperature uniformity • E...
Catalog: 4326270
Size: 500 plates

Silencer® Select GAPDH siRNA

Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls

Ambion® Silencer ® SelectGAPDH Positive Control siRNA, In Vivo Ready is extensively validated and an ideal control for many aspects of an siRNA experiment. The control is highly purified as required for animal use. 250 nmol is provided. • Premium-quality siRNA, highly purified and ready to use • Functionally tested in several common cell lines • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs? Silencer ® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algo...
Catalog: 4404024
Size: 250 nmol

PichiaPink™ Secreted Protein Kit

Category: Products & Services > Kits & Assays > Protein Expression Kits > Yeast Expression Kits

The PichiaPink™ Secreted Protein Expression Kit is a new recombinant protein expression system based on the yeast Pichia pastoris . The PichiaPink™ Secreted Protein Expression Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant protein. The PichiaPink™ Secreted Protein Expression Kit comes with our new Pichia strains, the pPINK-HC and pPINK-LC vectors, and secretion signal sequences for production of your protein. • Get started with secreted protein production in Pichia pastoris . • Screen your Pichia transformants rapidly by ...
Catalog: A11151
Size: 1 kit

StepOne™ Real-Time PCR System, 1-year Extended Warranty

Category: Products & Services > Instruments, Software, & Accessories > Instruments > Instrument Services

A 1-year Extended Warranty for your StepOne™ Real-Time PCR System will add 1 year of coverage to the existing warranty. With this extended warranty, your StepOne™ Real-Time PCR System is covered by our Rapid Exchange Service* protection plan. Should you encounter an issue with your instrument, call technical support and talk to one of our Certified Senior Technical Specialists to get assistance in resolving most technical problems. In the event that your instrument needs service, simply repackage the defective instrument and return it to us – we will pay the shipping costs. We will then send a...
Catalog: ZGEXSCSTEPONE
Size: 1 each

ViraPower™ HiPerform™ Lentiviral FastTiter™ TOPO® Expression Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The ViraPower™ HiPerform™ Lentiviral FastTiter™ TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti7.3/V5-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central ...
Catalog: K5320-00
Size: 1 kit

High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 1000 x 20 µL reactions. • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (for...
Catalog: 4374967
Size: 1000 reactions

ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit contains the MultiSite Gateway®-adapted ViraPower™ HiPerform™ promoterless lentiviral expression vector, pLenti6.4⁄R4R2⁄V5-DEST™ for easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any promoter of choice in dividing and non-dividing mammalian cells. The pLenti6.4⁄R4R2⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integras...
Catalog: A11146
Size: 20 reactions

Ambion® RNA-Seq Library Construction Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII is a single kit for RNA library construction of either whole transcriptome or small RNA (such as miRNA), for use in downstream next generation sequencing analysis. Designed as a complete solution with a common workflow, the kit optimally combines all necessary reagents for discovery of coding RNA, non-coding RNA, alternative splicing, small RNAs and isoforms, while preserving strand orientation. The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII platform is based on the same technology used in ...
Catalog: 4454073
Size: 1 kit

pLenti6.3/V5™-TOPO® TA Cloning® Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

Invitrogen's™ pLenti6.3⁄V5™-TOPO®Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00). The pLenti6.3⁄V5™-TOPO® -TA Cloning Kit contains the TOPO®- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5™-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequenc...
Catalog: K5315-20
Size: 1 kit

TaqMan® Array Mouse GPCR Panel

Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays

The Applied Biosystems® TaqMan® Mouse GPCR Array is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of mouse GPCR genes important in drug discovery. This product is designed with 380 TaqMan® Gene Expression Assays arrayed in singletons in 384 wells (plus 1 control arrayed in quadruplicate) and is compatible with an upgraded Applied Biosystems 7900HT Fast Real-Time PCR System. The targets include retinal receptors, small molecule receptors, chemokine receptors and others including classic endogenous control targets. This product is packaged in 1 box contai...
Catalog: 4378703
Size: 4 cards

iBlot® Gel Transfer Device

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Blotting Systems

The iBlot® Gel Transfer Device performs western blotting transfer efficiently and reliably typically in 7 minutes or less without the need for liquid buffers. The device is integral part of the iBlot® Western Blotting System. The system consists of the device and disposable blotting stacks that contain the buffers and the transfer membrane (nitrocellulose or PVDF), and offers high quality transfer, convenience and speed. The device and the stacks are sold separately. The iBlot® system is ideal for scientists analyzing proteins through western blotting who want: • Complete protein transfer in s...
Catalog: IB1001
Size: 1 each

5X mMESSAGE mMACHINE® T7 ULTRA Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

The Ambion® mMESSAGE mMACHINE™ T7 Ultra Kit combines a new cap analog—Anti-Reverse Cap Analog (ARCA)—with Ambion's patented high-yield transcription technology, to generate RNA transcripts that produce higher protein yields compared to other transcripts upon translation. The kit includes sufficient reagents for 50 reactions. • Synthesize more protein from in vitro –transcribed RNA • Express RNA transcripts more efficiently both in vitro and in vivo • Stabilize transcripts in vivo with included reagents for poly(A) tailing ARCA-capped RNA is Translationally More Active: A base modification in A...
Catalog: AMB1345-5
Size: 50 reactions

Ion Xpress™ Barcode Adapters 1-16 Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Catalog: 4471250
Size: 1 kit