Enrich epithelial tumor cells directly from whole blood, bone marrow, or PBMC suspensions. Enriched tumor cells are lysed for mRNA isolation and RT-PCR amplification. The sensitivity of combined enrichment and RT-PCR detection can be as high as 1 tumor cell in 1 ml blood. Depletion of epithelial tumor cells from whole blood/bone marrow or PBMC. Positive isolation of epithelial tumor cells for downstream molecular analysis. No columns required Starting samples: Whole blood, bone marrow, PBMC, buffy coat. Number of cells processed: 200 ml whole blood or ~4 × 10 9 PBMC.
Size: 5 ml
Our Magnetic Particle Concentrators (MPC®) enable optimal binding of Dynabeads® magnetic beads with their targets (e.g., cells, proteins, and nucleic acids) in a variety of starting samples. The MPC®-S was designed specifically for microbiology applications; it has been validated with select Dynabeads® for enrichment of E. coli O157:H7, Salmonella, non-O157 STEC, Cryptosporidium, and Giardia. The MPC®-S can be used with both food enrichment samples types as well as water samples. It holds up to six microcentrifuge tubes and has an optimal working volume range of 20 µl–2 ml. Note that this magn...
Size: 1 unit
An excellent tool for highly predictable and consistent extraction and isolation of nucleic acids from biological samples, following a simple magnetic separation protocol. Please note that this is a stand-alone product containing only Dynabeads® in a storage buffer. Our products Dynabeads® SILANE genomic DNA (Cat.no. 370-12D) or Dynabeads® SILANE viral NA (Cat.no. 370-11D) include the appropriate buffers and protocols you need to extract genomic DNA or viral nucleic acids respectively. Magnetic particles from alternative suppliers often have a random size range distribution, surface area and b...
Size: 5 ml
Streptavidin-coupled Dynabeads® is the gold standard for isolation and handling of biotinylated nucleic acids, antibodies or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10 -15 ) is utilized in a vast number of applications. These uniform and superparamagnetic beads are 1 µm in diameter, with a monolayer - not a multilayer - of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The monolayer of streptavidin leaves the vast majority of the biotin binding sites sterically available for bind...
Size: 10 ml
Dynabeads® Sheep-anti Rat IgG binds monoclonal rat IgGs of your choice for specific cell isolation or depletion. All cells from all species except from rat can be isolated with this product, depending only on the specific antibody of choice. Especially well suited for cell isolation from mouse samples. Depletion: cells can be depleted from a sample Positive isolation: cells can be positively isolated from a sample for molecular applications Negative isolation: cells can be negatively isolated from a sample (i.e. spleen or lymph node cells) by adding an antibody cocktail of rat IgGs towards the...
Size: 5 ml
The new DynaMag™-2 magnet is optimized for efficient magnetic separation in small sample volumes using Dynabeads®. The DynaMag™-2 magnet combines a strong magnetic attraction for efficient separations with a flexible and smart ergonomic design. The top rack can be easily removed from the magnet in the base, ready for resuspension, vortexing, rotation or manual sample shaking. A center pin in the rack ensures equal vortexing of all tubes. The rack can also be removed and used to store tubes. The magnet can be used with all types of Dynabeads® for efficient isolation of proteins and nucleic acid...
The DynaMag™-5 Magnet is intended for use with Dynabeads® for the immunomagnetic separation of cells. The DynaMag™-5 magnet accommodates tubes from 11–16 mm in diameter (e.g., flow cytometry tubes and most blood collection tubes), with an optimal working volume of 1–5 mL. The magnet holds 8 tubes and is compatible with all Dynabeads® (1–4.5 µM in diameter). When performing cell separation using Dynabeads®, a DynaMag™ magnet is required. Different magnets are optimized for different working volumes and tube sizes. See our complete overview of the DynaMag™ magnets . The DynaMag™-5 magnet contain...
Size: 8 samples
This purified E-Cadherin Monoclonal Antibody is specific to human E-Cadherin. The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells. Mouse anti- E-Cadherin monoclonal antibody can be used in applications such as western blotting, immunohistochemistry, immunocytochemistry/immunofluorescence, and immunoprecipitation. This anti- E-Cadherin monoclonal antibody recognizes the product of the CDH1 gene. Antibody Specifications: • Applications: Validated applications for this E-Cadherin antibody are western blotting, immunohistochemistry, and immunoprec...
Size: 100 µg
This rat anti-E-Cadherin monoclonal antibody is specific to mouse E-Cadherin. E-Cadherin monoclonal antibody recognizes the expressed product of the Cdh1 gene. The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells. In addition, the tissue specificity of cadherin subtypes are becoming valuable markers for the identification and differential of certain cancers. Validated applications for E-Cadherin monoclonal antibody are epitope mapping and inhibition of E-Cadherin-dependent cell-cell contact. Applications: validated for epitope mapping and inhib...
Size: 100 µg
The E-Gel® Camera Hood Kit, when combined with a base, forms the E-Gel® Imager System, which is a personal imaging system for documenting and analyzing agarose gels and E-Gel® cassettes. Each E-Gel® Camera Hood Kit includes a compact digital camera mounted in a sleek hood, a universal filter, power and USB cables, and two powerful software applications. There are three bases available for purchase that will work with the E-Gel® Camera Hood Kit: a UV Light Base, a Blue Light Base, and an E-Gel® Adaptor Base. In any of these three configurations, the E-Gel® Imager system provides a small and lig...
Size: 1 kit
The E-Gel® Safe Imager™ Real-Time Transilluminator allows you to watch migration of DNA in real-time in E-Gels containing SYBR® Safe. It offers: • Real-Time monitoring of DNA migration • Improved safety for the user • Enhanced cloning efficiency of retrieved DNA Real-Time Transilluminator Designed for Safety and Cloning The E-Gel® Safe Imager™ Real-Time Transilluminator is designed to fit perfectly beneath the E-Gel® iBase™ Power System and is intended for use with E-Gels containing SYBR® Safe. Blue light emitted by the instrument is ideal for viewing DNA that is stained with SYBR® Safe. Unlik...
Size: 1 each
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
Size: 4 pads
E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme. Applications: Second-strand cDNA synthesis (1). T4 DNA Ligase alternative when blunt-end ligation is not required (2). Source: Purified from E. coli 594 (Su - ) bearing λ lysogen gt4lop-11 lig + S7 (3). Performance and Quality Testing: Endodeoxyribonuclease and 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested. Unit Definition: One unit is the...
Size: 100 units
These individual EcoRI & MseI Adaptors can be used in the Restriction Ligation reaction. For Research Use Only. Not for use in diagnostics procedures.
Size: 100 reactions
EDAC is a water-soluble carbodiimide that can be used to crosslink biological substances that contain carboxylate acids and primary amines. Protocols for crosslinking molecules with this reagent can be found in Bioconjugate Techniques , by G.T. Hermanson (B-7884).
Size: 100 mg
EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a s...
Size: 5 g
This monoclonal antibody reacts with the extracellular domain of epidermal growth factor receptor (170 kDa). Clone 31G7 is useful in detecting the majority of squamous cell carcinomas that arise from both squamous epithelium and metaplastic squamous epithelium. Positive tissue: Breast carcinoma. Clone/PAD: 31G7 Immunogen: EGFr derived from A-431 cells Specificity: EGFr. No cross-reactivity observed with c-erbB-2. Reactivity: Human Applications: Immunohistochemistry (FFPE) Pretreatment: Epitope Retrieval: Not required , Enzyme Digestion: Required (Digest-All™ 3/Pepsin) Expected Staining Pattern...
Size: 2 ml
EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER.
Size: 10 µg
ElectroMAX™ DH10B™ T1 Phage-Resistant electrocompetent E. coli cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 10 10 cfu/ µg plasmid DNA in efficiency. They are ideal for applications requiring high efficiency transformation, including cDNA and gDNA library construction. • Maximizes yield of transformants from limited cloning products • Permits efficient cloning of methylated DNA • Resistant to T1 and T5 bacteriophages • Supports blue/white screening by α-complementation on plates containing X-Gal or Bl...
Size: 0.5 ml
The EnzChek® Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme acitivty in solution. The assay measures lysozyme activity on Micrococcus lysodeikticus cell walls, which are labeled to such a degree with fluorescein that the fluorescence is quenched. Lysozyme action can relieve this quenching, yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.
Size: 1 kit
ER-Tracker Blue-White DPX dye is a photostable probe that is selective for the endoplasmic reticulum (ER) in live cells. The cells can be aldehyde-fixed after staining with the dye but significant fluorescence signal will be lost. With excitation at ~374 nm, the environmentally-sensitive probe exhibits emission between 430 and 640 nm. A long-pass DAPI filter works best.
Size: 20 × 50 µl
The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Size: 100 µg
Variation in RNA expression data can be attributed to a variety of factors including the quality of the starting material, the level of cellularity and RNA yield, the platform employed, and the person performing the experiment. To control for these sources of variability, a common set of external RNA controls has been developed by the External RNA Controls Consortium (ERCC), an ad-hoc group of academic, private, and public organizations hosted by the National Institute of Standards and Technology (NIST). The controls consist of a set of unlabeled, polyadenylated transcripts designed to be adde...
Size: 1 kit
The Molecular Probes® BioParticles® product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles® products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan ( S. cerevisiae ) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dy...
Size: 10 mg
Essential 8™ Medium (published as E8) is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson (published as "E8") and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and is proven to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and has been shown to support iPSC growth for >50 passages without any signs of karyotypic a...
Size: 500 ml
The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~528/617).
Size: 1 mg
The Applied Biosystems® Eukaryotic 18S rRNA Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan Gene Expression Assays, Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: 18S RefSeq: X03205.1 Probe Exo...
Size: 1000 reactions
Expi293™ Expression Medium is a chemically-defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density cultures of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression. • Supports growth of suspension 293 cultures to densities of over 1.6 x 10 7 cells/mL, with >95% cell viability • Transfection-compatible serum-free suspension culture medium allowing transfection efficiencies of greater than 70% using ExpiFectamine...
Size: 1000 ml
Affinity purified F(ab')2 gragment of goat anti-rat IgG conjugated to horseradish peroxidase. To minimize cross-reactivity, this antibody has been absorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Size: 0.5 mg
F4/80, Rat Anti-Mouse, (Biotin); Biotinylated Monoclonal antibody specific to Mouse F4/80. This antibody is validated for use in Flow Cytometry. Anti-F4/80 recognizes the expressed product of the Emr1 gene.
Size: 1 ml
Sera Category: Specialty Stripped with charcoal-dextran. Recommended storage condition: -5°C to -20°C Intended use(s): For research use only.
Size: 50 ml
Sera Category: Specialty Origin: United States. Endotoxin level: 50 EU/ml (Specification is checked and recorded; levels routinely Hemoglobin level: 25 mg/dl.
Size: 100 ml
Recombinant Mouse Fibroblast Growth Factor Basic (also known as FGF Basic, bFGF, FGF 2, FGF-β or Heparin Binding Growth Factor) is a bioactive protein intended for use in cell culture applications. FGF-basic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. • High purity —no interference from other proteins or contaminants • High biological activity —more results with less...
Size: 100 µg
FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit , which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. FISH Tag™ Kit Specificatio...
Size: 1 kit
The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors. FISH Tag™ Kit Specificati...
Size: 1 kit
Clone/PAD: ZF24 Immunogen: FITC Specificity: Specific for FITC. Reactivity: FITC Applications: WB, ELISA, Immunohistochemistry, Immunoprecipitation
Size: 100 µg
High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Size: 500 ml
Invitrogen offers a variety of tips for easy and accurate loading of samples. They are RNase/DNase-free and non-autoclavable.
Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. The Flp-In™ T-REx™-293 Cell Line contains pFRT⁄lacZeo and pcDNA™6⁄TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase ...
Size: 1 ml
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...
Size: 500 µg
The FluoReporter® FITC Protein Labeling Kit provides a convenient and flexible means for attaching fluorescein (FITC, Ex/Em ~494/518 nm) to your monoclonal or polyclonal antibody (or any protein >30 kDa). The kit supplies the reagents needed for 5 to 10 labeling and separation reactions. Important Features of the FluoReporter® FITC Protein Labeling Kit: • Labeled proteins typically ready to use in under 3 hours with very little hands-on time • Optimized for 0.2–2.0 mg of protein with molecular weight >30 kDa • Labeled proteins are purified using convenient spin filters • Requires the antibody ...
Size: 1 kit
Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, first to fluorescein monogalactoside (FMG) and then to highly fluorescent fluorescein. Enzyme-mediated hydorlysis of FDG can be followed by the increase in either absorbance or fluorescence.
Size: 5 mg
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Size: 25 mg
The lipophilic probe, FM™ 4-64FX exhibits low fluorescence in water but fluoresces intensely upon binding the outer leaf of the plasma membrane providing discrete plasma membrane staining. The binding is rapid and reversible and "FX" denotes that this analog is fixable with aldehyde-based fixatives. It is also an excellent reagent for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling.
Size: 10 × 100 µg
The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein. Free your lab from the time-consuming, labor-intensive process of seeding, feeding, passing, and maintaining multiple flasks of adherent cells (Figure 1). The FreeStyle™ 293 Expression System combines GIBCO® FreeStyle™ 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. FreeStyle™ 293-F cells and 293fectin™ are available as part of the Expression System or sep...
Size: 1 kit
The FreeStyle™ MAX System is a breakthrough technology for rapid and high-yield mammalian protein production. This new mammalian expression technology is available in two convenient formats. FreeStyle™ MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle™ cells with high efficiency. The FreeStyle™ MAX Expression System combines GIBCO® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293 cells. The only equipment required to use the FreeStyle™ MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinne...
Size: 15 ml
Fungizone® antimycotic is a formulation of Amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension. Amphotericin B is used to prevent the growth of fungi by causing an increase in fungal plasma membrane permeability. Gibco® Fungizone® antimycotic is used for the prevention of yeast and multicellular fungi contamination of cell cultures. Gibco® Fungizone contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per ml in distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/ml. Life Technologies offers a variety of antibi...
Size: 20 ml
Fura-FF pentapotassium salt is a fura-2 derivative that has Ca 2+ sensitivity similar to mag-fura-2, but is relatively insensitive to Mg 2+ . This water-soluble salt form is useful for intracellular loading by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Size: 500 µg
Mouse anti-Galectin-1; Monoclonal antibody specific to Human, mouse, swine, rat, ovine, hamster, bovine Galectin-1.This antibody is validated for use in Western Blot, Immunocytochemistry/ Immunofluorescence.
Size: 100 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Size: 6 µg