Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards
Catalog: 50-197Z
Size: 15 ml
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > dNTPs
This dNTP Set consists of all four deoxyribonucleoside triphosphates (dATP, dCTP, dGTP, dTTP), each at a concentration of 100 mM. They are suitable for use in PCR, sequencing, nick translation, fill-in, cDNA synthesis, and TdT tailing reactions, as well as for dilution of radiolabeled dNTPs.
Catalog: 10297-018
Size: 4 × 25 µmol
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers
BlueJuice™ Gel Loading Buffer is designed for easy loading and tracking of DNA samples in agarose or native polyacrylamide gels.
Catalog: 10816-015
Size: 3 × 1 ml
Category: Products & Services > Buffers & Chemicals
Catalog: AM8703G
Size: 200 µl
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Running Buffers
Molecular biology grade, Ambion® 10X TBE solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating...
Catalog: AM9865
Size: 4 bottle
Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers
The 1X TE Buffer is a component in the PureLink™ 96 Plasmid Purification System. The 1X TE buffer is used to resuspend the final purified plasmid pellet and contains very low EDTA, so it is compatible with sequencing and other enzymatic applications.
Catalog: 12090-015
Size: 100 ml
Category: Products & Services > Instruments, Software, & Accessories
Catalog: 4305936
Size: 500 tubes
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Metabolic Assay Kits
2-NBDG is a fluorescent glucose analog that has been used to monitor glucose uptake in live cells, as an indicator of cell viability. Although sensitive to its environment NBD fluorescence typically displays excitation/emission maxima of ~465/540 nm and can be visualized using optical filters designed for fluorescein.
Catalog: N13195
Size: 5 mg
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > 2-Mercaptoethanol
Gibco® 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. Mercaptoethanol is not stable in solution so most protocols require daily supplementation. Gibco® 2-Mercaptoethanol is made at 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). Product Use For Research Use Only. Not for use in diagnostic procedures. cGMP Manufacturing and Quality System Gibco® 2-Mercaptoethanol is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the...
Catalog: 31350-010
Size: 20 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
Application(s): For growth of hosts for replication of M13 vectors. Instructions for use: 1. Suspend 31 g in 1 L of demineralized water. 2. Autoclave for 15 min at 121°C.
Catalog: 22712-020
Size: 500 g
Category: Products & Services > Buffers & Chemicals > General Buffers > SSC Buffers
Molecular biology grade, Ambion® 20X SSC solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating non...
Catalog: AM9770
Size: 500 ml
Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers
The RNase-free TE buffer concentrate, 20X TE buffer , which is essential to the success of the RiboGreen® RNA quantitation assays (R11491, R37302) is available separately from the kits to extend the number of low-concentration assays.
Catalog: T11493
Size: 100 ml
Category: Products & Services > Eukaryotic Cells > Cell Lines > Mammalian Cell Lines
The 293FT Cell line is ideal for generating high-titer lentivirus using the ViraPower™ Lentiviral Expression System. The 293FT Cell Line is a fast-growing, highly transfectable clonal isolate derived from human embryonal kidney cells transformed with the SV40 large T antigen. When a ViraPower™ expression vector and the ViraPower™ Lentiviral Packaging Mix are co-transfected into 293FT cells, high levels of the viral RNA and the gag⁄pol and rev proteins required for packaging are produced. Advantages • Fast growing • Highly transfectable • Produces high viral titers • Allows very high levels of ...
Catalog: R700-07
Size: 3 × 10^6 cells
Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals
Ambion® Molecular biology grade, 3 M Sodium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for cont...
Catalog: AM9740
Size: 100 ml
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sequencing & Genetic Analyzer Instruments
The 310 Genetic Analyzer is an automated single-capillary genetic analyzer designed for a wide range of sequencing and fragment analysis applications. • Perform comparative sequencing, linkage analysis, STR analysis, SNP detection, discovery and validation, mutation detection, and many other applications. • Eliminate gel preparation. • Set up runs in minutes. • Automate your workflow to minimize hands-on time and costs. • Detect five dyes simultaneously to increase throughput. Versatile, Affordable, Easy-to-Use, and Almost Hands-Free The 310 Genetic Analyzer – the ideal alternative to gel-base...
Catalog: 310-00-100-120WR
Size: 1 instrument
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards
The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: 4404312
Size: 4 tubes
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Thiol-Reactive Fluorophores, Biotins & Other Labels
Our 4-acetamido-4'-maleimidylstilbene-2,2'- disulfonic acid is a thiol-reactive reagent that is water soluble, with high polarity and membrane impermeability. This polysulfonated dye is useful for determining whether thiol-containing proteins and polypeptide chains are exposed at the extracellular or cytoplasmic membrane surface.
Catalog: A-485
Size: 25 mg
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
DASPMI (4-Di-1-ASP) is a styryl dye stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane potential.
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
The cationic mitochondrial dye, 4-Di-2-ASP is a styryl with a large Stokes shift. It can also be used to stain presyntptic nerve terminals independent of neuronal activity.
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
DNDS is a stilbene sulfonate that can be used to inhipit anionic transport.
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose
Gibco® 4% Agarose is an autoclaved, high purity, low melting point gel designed for plaque assays and the purification of baculovirus. The 4% Agarose Gel melts at 65 – 70°C and remains fluid at 37°C. When diluted with concentrated insect cell culture medium, Gibco® 4% Agarose produces a gel firm enough to immobilize local infections, yet ductile enough to allow for the efficient removal of plaques for clonal expansion. Product Use For Research Use Only. Not for use in diagnostic procedures.
Catalog: 18300-012
Size: 40 ml
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores
Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pK a of 4.8, this dye can be used as an acidic pH sensor.
Catalog: C-369
Size: 100 mg
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > BrdU, EdU & EU Reagents
Cells can naturally incorporate the thymidine analog BrdU into their cells during cell division, making this nucleoside analog an excellent marker of by cell cycle and proliferation. Analysis of incorporated BrdU can be with an anti-BrdU antibody.
Catalog: B23151
Size: 100 mg
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > BrdU, EdU & EU Reagents
Detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide, ethynyl uridine (EU, E10345) or in kit form with Click-iT Nascent RNA capture kit, C-10365. Both approaches use powerful click chemistry detection, a simple and robust two-step technique. In step one, the alkyne-containing biomolecule is fed to cells or animals and actively incorporated into RNA. Detection utilizes the chemoselective ligation or “click" reaction between an azide and an alkyne where the modified protein is detected with a corresponding azide-containing dye or hapt...
Catalog: E-10345
Size: 5 mg
Category: Products & Services > Oligos, Primers, Probes & Nucleotides
5’ Phosphate labeled oligo
Catalog: 4326342
Size: 80,000 pmol
Category: Products & Services > Oligos, Primers, Probes & Nucleotides
5’-Fluorescent labeled oligo: 10,000 picomoles
Catalog: 450007
Size: 10,000 pmol
Category: Products & Services > Buffers & Chemicals > Detergents & Solubilizers
5% Digitonin is a detergent solution used to prepare native protein samples for use with the NativePAGE™ Gel System. Also included in the NativePAGE™ Sample Prep Kit, this ready-to-use detergent solution improves solubility of hydrophobic and membrane proteins during sample preparation. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.
Catalog: BN2006
Size: 1 ml
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Blotting Buffers
Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols. The solution contains 1% Ficoll (type 400), 1% polyvinylpyrrolidone, and 1% bovine serum albumin. Sterile filtered.
Catalog: 750018
Size: 100 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits
The Ambion® mMESSAGE mMACHINE™ T7 Ultra Kit combines a new cap analog—Anti-Reverse Cap Analog (ARCA)—with Ambion's patented high-yield transcription technology, to generate RNA transcripts that produce higher protein yields compared to other transcripts upon translation. The kit includes sufficient reagents for 50 reactions. • Synthesize more protein from in vitro –transcribed RNA • Express RNA transcripts more efficiently both in vitro and in vivo • Stabilize transcripts in vivo with included reagents for poly(A) tailing ARCA-capped RNA is Translationally More Active: A base modification in A...
Catalog: AMB1345-5
Size: 50 reactions
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Amine-Reactive Fluorophores, Biotins, Quantum Dots, & Other Labels
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: C-6164
Size: 10 mg
Category: Products & Services > Buffers & Chemicals > General Chemicals > Oligo Synthesis Reagents
Contains 45 mg 6-FAM™ Dye Phosphoramidite. 6-FAM™ Dye Phosphoramidite is used for the 5’ attachment of FAM™ dye during oligonucleotide synthesis. 6-FAM™ Dye Phosphoramidite is an optically pure isomer (not a mixture). The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesi...
Catalog: 403169
Size: 45 mg
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Polyacrylamide Gels
Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Catalog: EC6365BOX
Size: 1 box
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Polyacrylamide Gels
Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Catalog: EC62655BOX
Size: 1 box
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Polyacrylamide Gels
Denaturing polyacrylamide TBE-Urea Gels resolve single-stranded DNA oligos or RNA into sharp, distinct bands. Novex® TBE-Urea Gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and Northern blot analysis. Novex® TBE-Urea Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE-Urea Gels are made with high-purity reagents, including tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEME...
Catalog: EC6865BOX
Size: 1 box
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Real-Time PCR Instrument Calibration Kits & Reagents
The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. T...
Catalog: 4350584
Size: 1 kit
Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software
The 21 CFR Part 11 Software Module for the 7500 Systems offers the flexibility of user-customizable security configuration settings to assist with 21 CFRp11 compliance. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4377355
Size: 1 kit
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Real-Time PCR Instrument Accessories
The 7900HT TaqMan® Array Upgrade enables you to run the TaqMan® Arrays – a microfluidic card – on your Applied Biosystems 7900HT Fast Real-Time PCR System. This upgrade gives you access to an efficient, easy-to-use tool that eliminates labor-intensive pipetting steps and enables high throughput gene expression results using the gold-standard 5' nuclease TaqMan® Assay technology. • Convenience – includes everything you need to run the 7900HT TaqMan® Array on your 7900HT system • Success – highly trained service engineers will install and test your 7900HT TaqMan® Array Upgrade to get you up and ...
Catalog: 4329012
Size: 1 kit
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Polyacrylamide Gels
Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Catalog: EC6215BOX
Size: 1 box