Products from Pcdna™3.3-Topo® Ta Cloning® Kit to Platinum® Pfx DNA Polymerase
The pcDNA™3.3-TOPO® vector is designed to deliver exceptionally high levels of transgene expression in adherent cells as well as in the FreeStyle™ MAX CHO and FreeStyle™ MAX 293 suspension cell systems. The pcDNA3.3™-TOPO® TA Cloning® Kit is supplied with the TOPO®-adapted plasmid vector, all reagents for cloning, and One Shot® TOP10 competent cells. The pcDNA™3.3-TOPO® vector offers the following benefits: • TOPO® adapted for cloning PCR products with >85% efficiency • High-level gene expression in a wide range of mammalian cells due to the native cytomegalovirus (CMV) immediate-early promote...
Catalog: K8300-01
Size: 20 reactions
Size: 20 reactions
pcDNA™4/HisMax is specifically designed to maximize protein expression in a variety of mammalian cells. The vector contains the QBI SP163 translational enhancer to increase expression levels two- to five-fold above those seen with the promoter alone (Figure 1). In addition to SP163-enhanced expression, pcDNA™4/HisMax includes a cleavable N-terminal Xpress™ tag for rapid detection of recombinant protein with an Anti-Xpress™ Antibody (Figure 2). pcDNA™4/HisMax is available TOPO® Cloning-ready for five-minute cloning of Taq amplified PCR products.
Catalog: V864-20
Size: 20 µg
Size: 20 µg
A Tetracycline-Regulated Expression System without Viral Transactivators The T-REx™ System yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2). Specific Activation The T-REx™ System uses a repressor mechanism that blocks transcription from the powerful CMV promoter in the absence of tetracycline. Because the T-REx™ System...
Catalog: V1020-20
Size: 20 µg
Size: 20 µg
All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V861-20
Size: 20 µg
Size: 20 µg
pcDNA5⁄FRT vector is a 5.1 kb expression vector designed for use with the Flp-In™ System. This vector features: 1. CMV promoter for high level expression in mammalian cells; 2. ten unique restriction site for easy cloning; 3. FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ host cell line and 4. Hygromycin resistance gene for selection of stable cell lines.
Catalog: V6010-20
Size: 20 µg
Size: 20 µg
pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid, pcDNA™5⁄FRT⁄TO⁄CAT, is also included for use as a positive control for transfection and expression in your Flp-In™ T-REx™ host cell line of choice. The pcDNA™5⁄FRT⁄TO vector contains the following elements: • A hybrid human cytomegalovirus (CMV)⁄TetO2 promoter for high-level, tetracycline-regulated expression of the gene of interest in a wide range of mammalian cells • Multiple cloning site with 10 unique restriction sites to facilitate cloning the gene of interest ...
Catalog: V6520-20
Size: 20 µg
Size: 20 µg
pcDNA™5/TO is a 5.7 kb expression vector designed for use with the T-REx™ System (Catalog nos. K1020-01 and K1020-02) available from Invitrogen. The vector allows tetracycline-regulated expression of the gene of interest in mammalian host cells expressing the Tet repressor (TetR) from the pcDNA™6/TR vector (Catalog no. V1025-20). Details about the pcDNA™5/TO vector can be obtained from the manual that can be downloaded from our website with V1025-20.
Catalog: V1033-20
Size: 20 µg
Size: 20 µg
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-DEST vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression • att R sites for Gateway® cloning, enabling recombination with att L-flanked fragments •C-terminal V5 tag for easy detection •Blasticidin resistance gene for efficient stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli Gateway® Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® d...
Catalog: 12489-027
Size: 6 µg
Size: 6 µg
All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V221-20
Size: 20 µg
Size: 20 µg
The pcDNA™6⁄TR vector is part of the T-REx™ System (catalog K102001), which yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2). The regulatory vector, pcDNA™6⁄TR, is provided for high-level expression of the tetracycline repressor (TR) protein. This vector expresses the Blasticidin resistance gene for rapid selection of ...
Catalog: V1025-20
Size: 20 µg
Size: 20 µg
These vectors are designed for high-level, constitutive expression from either the CMV or RSV promoters. Both vectors contain the EBNA-1 gene for episomal expression in primate and canine cell lines.
Catalog: V044-50
Size: 20 µg
Size: 20 µg
The pShooter™ vectors are designed to localize recombinant proteins to the nucleus, mitochondria, endoplasmic reticulum, or cytoplasm. In addition to the localization signal, each pShooter™ vector contains: Strong mammalian promoter (CMV or EF-1É) for constitutive expression of your protein of interest C-terminal c- myc epitope for detection with an Anti- myc Antibody Bovine Growth Hormone (BGH) polyadenylation site for mRNA stability f1 origin for single-stranded rescue of sense DNA Neomycin resistance gene for selection of mammalian cells with Geneticin¤ selection agent Ampicillin resistance...
Catalog: V821-20
Size: 20 µg
Size: 20 µg
Blasticidin is a potent selection agent that allows selection of stable mammalian cell lines in as little as one week. The BsdCassette™ vectors simplify the construction of custom vectors that use Blasticidin resistance as a selectable marker. Each BsdCassette™ vector is designed for use in a specific host system and offers: A strong host promoter to drive expression of the Blasticidin resistance gene ( bsd )(Table 1) Two large polylinkers flanking the promoter and bsd gene to facilitate the construction of Blasticidin resistant vectors Each BsdCassette™ vector carries the synthetic EM-7 promo...
Catalog: V510-20
Size: 20 µg
Size: 20 µg
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1). PCR SuperMix contains recombinant Taq DNA polymerase for routine PCR of fragments up to 5kb.
Catalog: 10572-014
Size: 100 reactions
Size: 100 reactions
The pCR®8/GW/TOPO® TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway® recombination cloning vector platform. Our pCR®8 TOPO® TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector. With our patented att sites, the pCR®8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time. The pCR®8/GW/TOPO® TA Cloning Kit offers: att sites for rapid recombination into a variety of Gateway...
Catalog: K250020
Size: 20 reactions
Size: 20 reactions
PCR8/GW/TOPO
Catalog: K252020SP
Size: 20 reactions
Size: 20 reactions
PCR8⁄GW⁄TOPO TA KIT
Catalog: 450642
Size: 1 each
Size: 1 each
PCRII TOPO TA Cloning Kit
Catalog: 10351021
Size: 20 reactions
Size: 20 reactions
Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR product is directionally cloned with efficiencies typically >99%. The pDONR™/Zeo vector haa a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
Catalog: 12535-035
Size: 6 µg
Size: 6 µg
Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR product is directionally cloned with efficiencies typically >99%. The pDONR™221 vector haa a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
Catalog: 12536-017
Size: 6 µg
Size: 6 µg
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V921-20
Size: 20 µg
Size: 20 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: V6020-20
Size: 6 µg
Size: 6 µg
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V962-20
Size: 20 µg
Size: 20 µg
A suite of accessories has been designed to enable research on living cells using your ArcturusXT™ LCM Instrument. Researchers may use slides or Petri dishes to perform imaging and microdissection experiments. Following a validated protocol from Arcturus® LCM Systems from Applied Biosystems—the leaders in tissue-based microdissection—the live cells may be isolated, remaining viable for subsequent re-culture or direct processing for use in downstream applications. Polyethylene naphthalate (PEN) Membrane Frame Slides for Live Cell Microdissection • Specially optimized for use with live cell expe...
Catalog: LCM0531
Size: 25 each
Size: 25 each
ARCTURUS® laser capture microdissection (LCM) instruments offer a unique combination of infrared (IR) laser capture and ultraviolet (UV) laser cutting, permitting the use of any slide type and any sample preparation for microdissection. While plain glass slides are effectively used for standard IR-based LCM, polyethylene naphthalate (PEN) membrane slides facilitate this Arcturus®-exclusive dual laser function and allow you to determine the appropriate sample preparation based on your application need. Plain Glass Slides The ARCTURUS® instruments are the only LCM systems that allow efficient us...
Catalog: LCM0522
Size: 50 each
Size: 50 each
The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibit...
Catalog: 15070-063
Size: 100 ml
Size: 100 ml
PENTR/D-TOPO CLONING KIT
Catalog: K240020SP
Size: 20 reactions
Size: 20 reactions
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryoti...
Catalog: A10462
Size: 10 µg
Size: 10 µg
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in...
Catalog: A10463
Size: 10 µg
Size: 10 µg
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in...
Catalog: A10464
Size: 10 µg
Size: 10 µg
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryotic systems • Ribosome bindi...
Catalog: A10465
Size: 10 µg
Size: 10 µg
The pENTR™/SD/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally into the pENTR™/SD/D-TOPO® entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes. The kit comes with everything necessary to clone and select your PCR amplified gene of interest. Gateway® System Ready - Rapidly shuttle cloned genes between multiple ve...
Catalog: K2420-20
Size: 20 reactions
Size: 20 reactions
The pFastBac™ Dual vector features two promoters in a single vector for expression of two proteins simultaneously in insect cells when using the Bac-to-Bac® Baculovirus Expression System. The vector has two strong promoters, the polyhedrin promoter and the p10 promoter, for high-level expression. An expression control is included that expresses both CAT and β-glucuronidase (gus).
Catalog: 10712-024
Size: 10 µg
Size: 10 µg
Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Catalog: 23600042
Size: 1 x 10L
Size: 1 x 10L
Ambion® Acid Phenol:Chloroform:IAA (25:24:1) is premixed and supplied at pH 6.6 (pH 7.9 ± 0.2), with included buffer. Acid Phenol:Chloroform:IAA is provided in one bottle of 100 mL. In RNA and DNA extraction procedures, Acid Phenol:Chloroform:IAA (pH 7.9) helps to stabilize the interface and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more conv...
Catalog: AM9730
Size: 100 ml
Size: 100 ml
pHrodo™ Red Avidin is a conjugate of avidin and pHrodo™ Red dye, a novel, fluorogenic dye that dramatically increases in fluorescence as the pH of its surroundings become more acidic. pHrodo™ Red dye conjugates are non-fluorescent outside the cell, but fluoresce bright red in phagosomes, making them ideal tools for studies ranging from phagocytosis of bioparticles to receptor internalization. Original pHrodo™ and pHrodo™ Red dye are the same molecule. With pHrodo™ Red Avidin Fluorogenic pH Sensor You Get: • Specific detection of phagocytosis and endocytosis • Faster staining • More accurate re...
Catalog: P35362
Size: 1 mg
Size: 1 mg
PI4KB and PI4KA are the two human type III PI4 kinases, which share sequence similarly with PI3 kinases and phosphorylate the 4 position of phosphotidyl inositol.
Catalog: PV5277
Size: 20 µg
Size: 20 µg
The pIB/V5-His-DEST vector is designed for rapid lambda phage site-specific recombination with a Gateway® entry clone and subsequent expression in Sf9, Sf21, or High Five™ insect cells. As part of the InsectSelect™ System, pIB/V5-His-DEST features: The OpIE 2 promoter for constitutive expression Blasticidin resistance gene driven by the weak GP64 promoter for selection of stable cell lines that have integrated the plasmid into a transcriptionally active site C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and easy purification with a ni...
Catalog: 12550-018
Size: 6 µg
Size: 6 µg
The PichiaPink™ Secreted Protein Expression Kit is a new recombinant protein expression system based on the yeast Pichia pastoris . The PichiaPink™ Secreted Protein Expression Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant protein. The PichiaPink™ Secreted Protein Expression Kit comes with our new Pichia strains, the pPINK-HC and pPINK-LC vectors, and secretion signal sequences for production of your protein. • Get started with secreted protein production in Pichia pastoris . • Screen your Pichia transformants rapidly by ...
Catalog: A11151
Size: 1 kit
Size: 1 kit
The PichiaPink™ Secreted Vector Kit contains the pPINKα-HC vector for use with the PichiaPink™ Yeast Expression System. The pPINKα-HC vector is built around the ADE2 gene for complementing the complementing the ade2 deletion in the PichiaPink™ strains. The pPINKα-HC vector has the α-mating factor secretion signal sequence so that all proteins are secreted. You can order the PichiaPink™ Secreted Vector Kit to refill your PichiaPink™ Yeast Expression System Kit. The PichiaPink™ Secreted Vector Kit comes with: • pPINKα-HC vector (high-copy number) (20 µg) • 5’α-factor sequencing primer (20 µg) • ...
Catalog: A11153
Size: 1 kit
Size: 1 kit
The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene. The PichiaPink™ Secretion Signal Set is designed for use with the pPINK-HC and pPINK-LC vectors. Optimize Secretion of Your Expressed Protein By adding one of the 8 secretion signal sequences to your gene, you can secrete your protein into the media to make it easier to harvest and purify. Pichia pastoris secretes few native proteins, so the bulk of the protein in the media will be yours. Each DNA sequ...
Catalog: A11155
Size: 8 each
Size: 8 each
The ARCTURUS® PicoPure® RNA Isolation Kit is designed to recover high-quality total RNA consistently from fewer than ten cells, even from a single cell. This kit is designed to provide consistent, efficient RNA recovery without sacrificing quality. Small volume elution allows you to maximize your recovery of RNA from small numbers of cells for use with your gene expression analysis. Key Product Features: • Greater sensitivty—isolate RNA from even a single cell • Superior efficiency—maximize RNA recovery from samples • Streamlined concentration—elute with small volumes • Flexible application—co...
Catalog: KIT0204
Size: 40 samples
Size: 40 samples
The Pippin Prep™ Kit 2010 contains 10 disposable pre-cast 3% agarose gel cassettes and a reagent pack for use isolating DNA fragments in the 50 - 300 bp range. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in three different formats. Streamlined Pippin Prep™ System DNA Size Selection Process The workflow for Pippin Prep™ DNA size selection consists of just a few simple steps:...
Catalog: 4472172
Size: 10 reactions
Size: 10 reactions
The Pippin Prep™ System is a small tabletop instrument that automates the size selection and collection of DNA for PGM™ sequencer libraries. Simply enter the desired base-pair target range via the software interface and after a 1.5 hr run the system will yield the programmed DNA fractions with virtually no low molecular weight contamination. The tight fragment size distributions obtained provide a more uniform template for amplification. And the processing of samples is simplified through the use of disposable pre-cast agarose gel cassettes. Benefits of the Pippin Prep™ System • Time savings c...
Catalog: 4471271
Size: 1 each
Size: 1 each
The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of your gene of interest. This vector has the following features: The OpIE 2 promoter for constitutive expression The Zeocin™ resistance gene for rapid selection of stably transfected cell lines C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid...
Catalog: V8010-01
Size: 1 kit
Size: 1 kit
PKA REG 11BETA [pS114) PAb; Unconjugated Polyclonal antibody specific to Mouse, (Human, Rat) PKA IIβ [pS114] (regulatory domain). This antibody is validated for use in Western Blot. Anti-PKA IIβ [pS114] (regulatory domain) recognizes the expressed product of the PRKAR2B gene. Catalog number 44998G is same antibody as former catalog number 44998. 44998G contains 50% glycerol, which improves the stability and longevity of this antibody.
Catalog: 44-998G
Size: 10 blots
Size: 10 blots
PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can also be activated by phospholipids.
Catalog: PV3790
Size: 10 µg
Size: 10 µg
Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B™ E. coli , also has 14 non-selectable unique restriction endonuclease recognition sites. Unique cleavage sites for Hin d III and Cla I are found in the promoter of the tetracycline resistance gene. Insertion of DNA at either of these two sites usually results in loss of tetracycline resistance. Performance and Quality Testing: Purity, DNA structure, and selected restriction endonuclease fragmentation...
Catalog: 15367-014
Size: 25 µg
Size: 25 µg
Replacement Parts for Electrophoresis Apparatus
Catalog: EI9022
Size: each
Size: each
Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA Polymerase from Thermococcus species KOD with proofreading (3´a5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic hot-start method that improves PCR specificity (2,3) (Figure 1). PCR x Enhancer Solution is included for higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of Platinum® Pfx DNA Polymera...
Catalog: 11708-013
Size: 100 reactions
Size: 100 reactions
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