Category: Products & Services > Eukaryotic Cells > Stem Cells > Embryonic Stem Cells
Mouse Embryonic stem cells (ESCs) were isolated from mouse (Strain C57BL⁄6) embryos at Day E3.5. Before cryopreservation, the cells were tested for viability (>70% post-thaw viability), their ability to remain undifferentiated when cultured on mouse feeder cells as assayed by marker expression (Oct4, SSEA-1, Nanog) and their ability to differentiate into embryoid bodies and express markers indicative of three germ layers (Ectoderm: (+) for β3-tubulin and nestin, Mesoderm: (+) for smooth muscle actin, Endoderm: (+) for AFP). Cells were cryopreserved at passage 20 (P20). For optimal growth perfo...
Catalog: S1503-100
Size: 1 ml
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores
To permit simultaneous long-term tracing of mixed-cell populations using different fluorescent colors, we have developed a probe whose applications should be similar to those of CFDA SE (commonly called CFSE). Cells labeled with our SNARF-1 carboxylic acid, acetate, succinimidyl ester have orange-red fluorescence that can be easily distinguished from that of cells loaded with green-fluorescent tracers such as CFDA SE.
Catalog: S22801
Size: 10 × 50 µg
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. • Impermeant to live cells • Excitation/Emission: 504/523 nm • Use with 488 Argon-ion laser • Works with mammalian cells and both Gram-positive and Gram-negative bacteria Simple Viability Determination SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, ...
Catalog: S7020
Size: 250 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
Streptavidin-Horseradish Peroxidase (HRP) conjugate
Catalog: SA100-01
Size: 50 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
The pcDNA™6 BioEase™ Gateway® Biotinylation System provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pcDNA™6/BioEase™-DEST vector (Figure 1) incorporates a 72 amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. This K. pneumoniae tag is recognized and efficiently biotinylated in vivo by a native mammalian system similar to the enzyme Biotin Protein Ligase (BPL) in E. coli (1). Proteins produced from the pcDNA™6/BioEase™-DEST vector are expressed as fusion proteins to this sequen...
Catalog: SA100-02
Size: 50 µl
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Salmonella enterica spp. Real-Time PCR Kit TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for R...
Catalog: SALMSPP
Size: 100 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Salmonella enterica spp. Real-Time PCR Kit (SALMSPP/50) TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzym...
Catalog: SALMSPP50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Screening Pack - Ruminant Abortion - Real-Time PCR Kit Supplied as 8 mixes distributed in strip tubes. TaqMan® probes - DNA Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master...
Catalog: SARP
Size: 96 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Schmallenberg Virus - S Gene - Real-Time PCR Kit (SBVS/50) TaqMan® probes - RNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT en...
Catalog: SBVS50
Size: 50 tests
Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards
Internal reference serum for use with indirect ELISA. For veterinary use only. Regulatory requirements vary by country; products may not be available in your geographic area. Biological hazard safety WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as glov...
Catalog: SCIV
Size: 100 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Two-well Indirect ELISA kits enable sensitive and specific detection of pathogen-specific antibodies in animal samples, allowing for reliable and rapid screening and detection of seropositive animals. How the kit works: 1. Samples are added to the specific antigen-coated microplate. If present in the sample, specific anti-pathogen antibodies bind to the antigen and form antigen-antibody complexes. 2. After washing, an anti-species peroxidase-labeled conjugate is added, which binds to any antibodies attached to the plate. 3. After a second washing, a chromogenic substrate is added. Color develo...
Catalog: SPIV2
Size: 96 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI™ VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits) • 1 external positive control of the same nature as the target (inactivated bacteria or virus). Us...
Catalog: SRPR
Size: 25 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Two-well Indirect ELISA kits enable sensitive and specific detection of pathogen-specific antibodies in animal samples, allowing for reliable and rapid screening and detection of seropositive animals. How the kit works: 1. Samples are added to the specific antigen-coated microplate. If present in the sample, specific anti-pathogen antibodies bind to the antigen and form antigen-antibody complexes. 2. After washing, an anti-species peroxidase-labeled conjugate is added, which binds to any antibodies attached to the plate. 3. After a second washing, a chromogenic substrate is added. Color develo...
Catalog: SRSV2
Size: 96 tests
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The Texas Red-X goat anti—rabbit IgG is labeled with our bright, photostable, red-fluorescent dye (which contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and the antibody). The antibody is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize crossreactivity, it has been adsorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Catalog: T-6391
Size: 0.5 ml
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Other Reactive Probes
The red-fluorescent tetramethylrhodamine (TAMRA) alkyne can be reacted with azides via a copper-catalyzed click reaction. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the ...
Catalog: T10183
Size: 0.5 mg
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
Trypan blue stain is a well-accepted method for staining dead cells. It can enter into cells with compromised membranes and is the method used with the Countess® automated cell counting platform.
Catalog: T10282
Size: 2 × 1 ml
Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers
The RNase-free TE buffer concentrate, 20X TE buffer , which is essential to the success of the RiboGreen® RNA quantitation assays (R11491, R37302) is available separately from the kits to extend the number of low-concentration assays.
Catalog: T11493
Size: 100 ml
Category: Products & Services > Buffers & Chemicals > Immunoassay Buffers & Reagents > ELISA Buffers & Reagents
This Casein-based blocking reagent can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems. For Research Use Only. Not for use in diagnostics procedures.
Catalog: T2015
Size: 30 g
Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chemiluminescent Substrates
This concentrated Nitro-Block-II™ Enhancer can be used with CSPD® or CDP-Star® chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II™ membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF membranes. • Chemically optimized formulations provide enhanced signal intensity in blotting applications with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates) on nitrocellulose or PVDF membranes. • Enhancer increases signal intensity, allowing decreased signal acquisition time. Increa...
Catalog: T2184
Size: 20 ml
Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents
Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 591/608 nm Superior to antibody staining Optimal for fixed and permeabilized samples Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually i...
Catalog: T7471
Size: 300 units
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Triplex BTV - All genotypes & BTV1 - Real-Time PCR Kit TaqMan® probes - RNA - triplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzym...
Catalog: TBTV1G
Size: 100 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Triplex BTV - All genotypes & BTV1 - Real-Time PCR Kit TaqMan® probes - RNA - triplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzym...
Catalog: TBTV1G50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Triplex BTV - All genotypes & BTV8 - Real-Time PCR Kit TaqMan® probes - RNA - triplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzym...
Catalog: TBTV8G
Size: 100 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Triplex BTV - All genotypes & BTV8 - Real-Time PCR Kit TaqMan® probes - RNA - triplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzym...
Catalog: TBTV8G50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Triplex Coxiella burnetii & Chlamydophila spp. Real-Time PCR Kit (TFQQCHP-CY5) TaqMan® probes - DNA - triplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR ...
Catalog: TFQQCHP
Size: 100 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Triplex Coxiella burnetii & Chlamydophila spp. Real-Time PCR Kit (TFQQCHP-CY5/50) Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI™ VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits) •...
Catalog: TFQQCHP50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Triplex Pasteurella multocida & Mannheimia haemolytica Real-Time PCR Kit (TPMMHP/50) TaqMan® probes - DNA - triplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-tim...
Catalog: TPMMHP50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI™ VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits) • 1 external positive control of the same nature as the target (inactivated bacteria or virus). Us...
Catalog: TQCRGIA50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Triplex bRSV & PI3 Real-Time PCR Kit TaqMan® probes - RNA - triplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA ki...
Catalog: TRSVPI350
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Mycobacterium tuberculosis Complex Real-Time PCR Kit TaqMan® probes - DNA - simplex - Two well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme ...
Catalog: TUB2W100
Size: 100 tests