Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits
The Qubit® (previously known as Quant-iT™) dsDNA BR Assay Kit is designed specifically for use with the Qubit® 2.0 Fluorometer . The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer. Common contaminants, such as salts...
Catalog: Q32850
Size: 100 assays
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need—including your choice of antibiotics and optional X-gal/IPTG—to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a...
Catalog: Q601-20
Size: 20 each
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need—including your choice of antibiotics and optional X-gal/IPTG—to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a...
Catalog: Q611-20
Size: 20 each
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Cryptosporidium parvum (QCRYP) Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI™ VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits) • 1 external positive control of the same nature as ...
Catalog: QCRYP50
Size: 50 tests
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The rabbit anti—goat IgG, horseradish peroxidase (HRP) conjugate has been adsorbed against human and rat serum proteins prior to labeling to minimize crossreactivity.
Catalog: R-21459
Size: 1 mg
Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics
Gibco® Zeocin™ selective reagent is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus . Gibco® Zeocin™ selective reagent acts by binding to and cleaving DNA, leading to cell death. Gibco® Zeocin™ selective reagent is used for selection of yeast, bacterial and mammalian cell cultures. This product is supplied at 100 mg/ml in sterile water and is used at a working concentration ranging between 50 - 400 µg/ml depending on the cell type. Life Technologies offers a variety of antibiotics and antimycotics for cell culture applications. Product Use For Res...
Catalog: R250-05
Size: 50 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits
The RediPlate™ 96 RiboGreen® RNA Quantitation Kit is a rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assay. The microplate has been pre-loaded with RiboGreen® Reagent, which shows bright green fluorescence upon binding to RNA or DNA (1). You simply add buffer and samples to the microplate wells, wait 10 minutes, and then read the fluorescence in any standard fluorescence-based microplate reader. The versatile RediPlate™ 96 design is ideal for either high-throughput quantitation or the measurement of a small number of samples. The RediPlate™ 96 RiboGreen® RNA Quantitat...
Catalog: R32700
Size: 1 kit
Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Phosphatase Assay Kits
The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensur...
Catalog: R33700
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
NucRed™ Live 647 ReadyProbes™ Reagent is a cell-permeant nuclear stain that emits bright, far-red fluorescence when bound to DNA. Like other ReadyProbes™ reagents, NucRed™ Live 647 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. NucRed™ Live 647 ReadyProbes™ Reagent provides: • Exceptionally bright far-red fluorescence well separated from blue, green, and red fluorophores • Rapid staining of live or fixed cells without wash steps • Ready-to-use liquid formulation in convenient dropper ...
Catalog: R37106
Size: 1 kit
Category: Products & Services > Buffers & Chemicals > Microscopy Reagents
Image-iT® FX Signal Enhancer ReadyProbes™ Reagent dramatically reduces background fluorescence resulting from non-specific staining during secondary detection using conjugates of streptavidin or secondary antibodies. This reagent is applied directly to slides or coverslips containing fixed and permeabilized cell or tissue samples prior to staining with fluorescent probes. Features of Image-iT® FX Signal Enhancer ReadyProbes™ Reagent include: • Easy protocol—apply ready-made solution using a dropper bottle • Compatible with the other steps in your immunostaining protocol • Effective with most f...
Catalog: R37107
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
Propidium iodide is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Propidium iodide is also used as a counterstain in multicolor fluorescent techniques. With Propidium Iodide ReadyProbes™ Reagent, we provide a room temperature-stable solution of classic DNA stain that comes in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of Propidium Iodide ReadyProbes™ Reagent: • Ready-to-use liquid propidium iodide formulation • Rapid staining of dead cells without wash steps • Ready-to-use liquid formulation in convenien...
Catalog: R37108
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
NucGreen™ Dead 488 ReadyProbes™ Reagent is a cell-impermeant stain that emits exceptionally bright, green fluorescence when bound to DNA. Cells that have lost plasma membrane integrity are stained within minutes, making this an extremely useful stain to estimate live/dead cell ratios and to measure cytotoxicity in kinetic live-cell assays. It is also suitable for staining nuclei in fixed cell preparations and tissue sections. Like other ReadyProbes™ reagents, NucGreen™ Dead 488 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and dr...
Catalog: R37109
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents
ActinGreen™ 488 ReadyProbes™ Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. Like other ReadyProbes™ reagents, the ActinGreen™ 488 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. ActinGreen™ 488 ReadyProbes™ Reagent provides: • High-affinity staining of F-actin with superior specificity compared to antibody methods • Outstanding fluorescence performance (Ex/Em: 495/518 nm) • Ready-to-use liquid formulati...
Catalog: R37110
Size: 1 kit
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Apoptosis Kits
CellEvent® Caspase-3/7 Green ReadyProbes™ Reagent is a fluorogenic, no-wash indicator of activated caspase-3/7 for live- and fixed-cell applications. Activation of caspase-3 is an early indicator of apoptosis and CellEvent® Caspase-3/7 Green reagent allows rapid and sensitive detection of cells destined for cell death. Like other ReadyProbes™ reagents, CellEvent™ Caspase-3/7 Green reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent provides...
Catalog: R37111
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents
ActinRed™ 555 ReadyProbes™ Reagent is a selective, high-affinity F-actin probe conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). Like other ReadyProbes™ reagents, ActinRed™ 555 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. ActinRed™ 555 ReadyProbes™ Reagent provides: • High-affinity staining of F-actin with superior specificity compared to antibody methods • Outstanding fluorescence performance (Ex/Em: 540/565 nm) • Ready-to-use liquid formulation in convenie...
Catalog: R37112
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
NucRed™ Dead 647 ReadyProbes™ Reagent is a cell impermeant stain that emits bright far-red fluorescence when bound to DNA. Cells that have lost plasma membrane integrity are stained within minutes, making this an extremely useful stain to estimate live/dead cell ratios and to measure cytotoxicity in kinetic live cell assays. NucRed™ Dead 647 reagent is also suitable for staining nuclei in fixed cell preparations and tissue sections. Like other ReadyProbes™ reagents, NucRed™ Dead 647 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip a...
Catalog: R37113
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue® Live ReadyProbes™ Reagent we have formulated this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Live ReadyProbes™ Reagent: Ready-to-use liquid formulation of high-purity Hoechst 33342 No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or cell cult...
Catalog: R37605
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes™ Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Fixed Cell ReadyProbes™ Reagent: Ready-to-use liquid DAPI formulation No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or...
Catalog: R37606
Size: 1 kit
Category: Products & Services > Columns, Resins & Plates > Spin Filters, Columns, & Purification Plates
The microcentrifuge spin columns included with the FastTrack® 2.0 and Micro-FastTrack™ 2.0 Kits are available separately. The columns are made from polypropylene and are designed to enhance the speed and efficiency of polyA + RNA isolation. The columns are sterilized and RNase-free.
Catalog: R500-20
Size: 200 columns
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers
Magnetic Stand is part of the I-SAGE kit
Catalog: R670-01
Size: each
Category: Products & Services > Eukaryotic Cells > Cell Lines > Insect Cell Lines
Gibco® Drosophila S2 cells are used for heterologous protein expression in the DrosophilaM Expression System (DES®). The S2 cell line was derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos. Many features of the S2 cell line suggest that it is derived from a macrophage-like lineage. S2 cells grow at room temperature in ambient CO 2 as a loose, semi-adherent monolayer in tissue culture flasks and in suspension in spinners and shake flasks. Drosophila S2 cells (frozen in Gibco® Schneider's Medium) feature: • Expression of protein transiently or through ...
Catalog: R690-07
Size: 1 ml
Category: Products & Services > Eukaryotic Cells > Cell Lines > Mammalian Cell Lines
The 293FT Cell line is ideal for generating high-titer lentivirus using the ViraPower™ Lentiviral Expression System. The 293FT Cell Line is a fast-growing, highly transfectable clonal isolate derived from human embryonal kidney cells transformed with the SV40 large T antigen. When a ViraPower™ expression vector and the ViraPower™ Lentiviral Packaging Mix are co-transfected into 293FT cells, high levels of the viral RNA and the gag⁄pol and rev proteins required for packaging are produced. Advantages • Fast growing • Highly transfectable • Produces high viral titers • Allows very high levels of ...
Catalog: R700-07
Size: 3 × 10^6 cells
Category: Products & Services > Eukaryotic Cells > Cell Lines > Mammalian Cell Lines
T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄ lac Z. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).
Catalog: R710-07
Size: 3 × 10^6 cells
Category: Products & Services > Eukaryotic Cells > Cell Lines > Mammalian Cell Lines
Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. The Flp-In™ T-REx™-293 Cell Line contains pFRT⁄lacZeo and pcDNA™6⁄TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase ...
Catalog: R780-07
Size: 1 ml
Category: Products & Services > Eukaryotic Cells > Cell Lines > Mammalian Cell Lines
The GripTite™ 293 MSR Cell Line is a genetically engineered Human Embryonic Kidney (HEK 293) cell line that expresses the human macrophage scavenger receptor and strongly adheres to standard tissue culture plates for dependable results. Developed from a 293-H subclone, GripTite™ 293 MSR cells show the same fast cell growth, high transfection efficiency, and high-level expression characteristics of the parental 293-H cells. Unlike most 293 cells, GripTite® 293 MSR cells dont wash away during the repeated manipulations of routine tissue culture or plate washing protocols (Figure 1). Their superi...
Catalog: R795-07
Size: 1 kit
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Epitope Tag & Reporter Specific Antibodies
This purified Mouse Anti-Xpress Monoclonal Antibody is designed to specifically interact with the Xpress™ leader peptide. The monoclonal antibody recognizes the amino acid sequence -Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys- . This Mouse Anti-Xpress™ Antibody has been tested for use in applications such as western blots, ELISA, and immunoprecipitation. Antibody Specifications: • Applications: Validated applications for Anti-Xpress™ Monoclonal Antibody are western blotting, immunoprecipitation, immunofluporescence, and ELISA. • Host Species and Isotype: The host species of the Xpress monoclonal antibody ...
Catalog: R910-25
Size: 50 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Epitope Tag & Reporter Specific Antibodies
This purified Mouse Histidine (C-term) Tag Monoclonal Antibody recognizes a polyhistidine amino acid sequence at the carboxy-terminus of proteins. The free carboxy-terminus of the terminal histidine residue is a key element of the epitope recognition site of the anti-His (C-term) Antibody. The Anti-His(C-term) Monoclonal Antibody recognizes the sequence: -His-His-His-His-His-His-COOH . Validated applications for Histidine (C-term) Tag monoclonal antibody are western blotting and ELISA. Antibody Specifications: • Applications: Validated applications for Histidine (C-term) Tag Monoclonal Antibod...
Catalog: R931-25
Size: 50 µl
Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards
Internal reference antigen for use with Ag capture ELISA. For veterinary use only. Regulatory requirements vary by country; products may not be available in your geographic area. Biological hazard safety WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as ...
Catalog: RBDG
Size: 50 tests
Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards
Internal reference serum for use with blocking ELISA. For veterinary use only. Regulatory requirements vary by country; products may not be available in your geographic area. Biological hazard safety WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as glov...
Catalog: RBVD
Size: 100 tests
Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards
Internal reference serum for use with indirect ELISA. For veterinary use only. Regulatory requirements vary by country; products may not be available in your geographic area. Biological hazard safety WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as glov...
Catalog: RCHL
Size: 100 tests