Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Yeast Expression Vectors
The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene. The PichiaPink™ Secretion Signal Set is designed for use with the pPINK-HC and pPINK-LC vectors. Optimize Secretion of Your Expressed Protein By adding one of the 8 secretion signal sequences to your gene, you can secrete your protein into the media to make it easier to harvest and purify. Pichia pastoris secretes few native proteins, so the bulk of the protein in the media will be yours. Each DNA sequ...
Catalog: A11155
Size: 8 each
Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits
The NCode™ VILO™ miRNA cDNA Synthesis Kit is part of a novel quantitative assay that provides flexibility, sensitivity, and specificity when quantitating miRNA expression. The system combines a carefully optimized polyadenylation reaction with the market-leading reverse transcriptase, SuperScript® III RT, in a universal first-strand cDNA synthesis reaction. Tried and tested SuperScript® III Reverse Transcriptase is formulated in an enhanced buffer system to provide you with the most reliable firststrand synthesis and higher cDNA yields, with sensitivity and accurate quantitation from 10pg to 1...
Catalog: A11193-050
Size: 50 reactions
Category: Products & Services > Kits & Assays > Genotyping Kits > HLA Typing Kits
Made to order SeCore GSSP's, composition and price to be determined via EvoQuest team and Genetic Systems Technical Support teams. The SeCore® HLA Sequencing System is intended for the identification and definition of Class I and II Human Leukocyte Antigens (HLA) and is for use in HLA typing. The SeCore® HLA Sequencing System provides human histocompatibility information of HLA Class I (A, B, and C) and Class II (DPB1, DQB1, and DR) loci using genomic DNA isolated from whole blood specimens. HLA typing using the SeCore® Sequencing Kits must be performed in the presence of an HLA Lab Director, ...
Catalog: A11256
Size: 1 kit
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Culture Matrices > Extracellular Matrix Coated Plates
Collagen I, Rat Tail is the cell culture workhorse of basic coating and gel preparations for growing attachment cells. Gibco® Collagen I, Rat Tail is now available in pre-coated tissue culture plates to help save time at the bench. Designed to meet a number of different applications, our high quality, consistent Collagen I Coated Plates come in 3 formats – 96 well, 24 well and 6 well plates . • Ready to use, room temperature storage • Quality tested to help ensure consistency • Available in 3 different plate formats and in custom formats for a broad range of applications Easy to use, easy to s...
Catalog: A11428-02
Size: 5 plates
Category: Products & Services > Labeling & Detection Products > BioConjugates > Protein Conjugates
Actin is an important tool for investigating cytoskeleton dynamics in-vivo . This highly purified actin from rabbit muscle is subjected to depolymerization and subsequent polymerization to ensure that the actin is able to assemble properly. The actin that polymerizes is then separated from remaining monomeric actin by centrifugation, depolymerized and packaged in monomeric form.
Catalog: A12375
Size: 1 mg
Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents
Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Selectively stains F-actin • Outstanding fluorescence performance • Excitation/Emission: 495/518 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples Get Superior Results in Your Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Flu...
Catalog: A12379
Size: 300 units
Category: Products & Services > Cell Culture & Transfection Reagents > Cryopreservation Media
Synth-a-Freeze® is a defined, liquid cryopreservation medium containing 10% dimethylsulfoxide (DMSO) without antibiotics, antimycotics, hormones, growthfactors, serum or protein and buffered with HEPES and sodium bicarbonate. Synth-a-Freeze is intended for freezing and storing a variety of mammalian cell types including human keratinocytes, embryonic stem cells, neural stem cells and mesenchymal stem cells. It should not be used for melanocytes.
Catalog: A12542-01
Size: 50 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Neuronal Cell Media
GIBCO® Astrocyte Medium has been specially formulated to support the growth of primary human and rat astrocytes while retaining their phenotypes. The medium has been internally validated and optimized to provide an optimal balance between cell growth and the maintenance of cell viability, normal phenotype, and function. If more rapid proliferation is desired, addition of an appropriate amount of epidermal growth factor (EGF) is recommended, although cells may exhibit morphological and phenotypic changes. Astrocytes are a type of glial cells of the nervous system with a typical star shape. It h...
Catalog: A12613-01
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents
Lipofectamine® LTX Reagent offers a new, advanced solution for gene expression studies in hard-to-transfect cells. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, difficult-to-transfect, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in experiments or cell lines. A certificate of origin may be obtained by contacting Technical Support. ...
Catalog: A12621
Size: 0.1 ml
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
Acridine Orange is a cell-permeant nucleic acid binding dye that emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This unique characteristic makes acridine orange useful for cell-cycle studies. Acridine orange has also been used as a lysosomal dye. This dye is available in solution (A-3568).
Category: Products & Services > Labeling & Detection Products > BioConjugates > Annexin V
In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including this FITC annexin V conjugate. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.
Catalog: A13199
Size: 500 µl
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Seamless Cloning Vectors
The GeneArt® Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt® Seamless Cloning and Assembly Kit (cat # A13288). The vector is manufactured by digesting supercoiled pUC19 plasmid with the restriction enzymes PstI and KpnI, and tested for cloning of up to 4 DNA fragments. Some of the key features and elements of the pUC19L vector include: • lacZα gene allows easy blue⁄white colony screening for selection of recombinants • pUC origin of replicatio...
Catalog: A13289
Size: 20 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers
Our Magnetic Particle Concentrators (MPC®) enable optimal binding of Dynabeads® magnetic beads with their targets (e.g., cells, proteins, and nucleic acids) in a variety of starting samples. The MPC®-S was designed specifically for microbiology applications; it has been validated with select Dynabeads® for enrichment of E. coli O157:H7, Salmonella, non-O157 STEC, Cryptosporidium, and Giardia. The MPC®-S can be used with both food enrichment samples types as well as water samples. It holds up to six microcentrifuge tubes and has an optimal working volume range of 20 µl–2 ml. Note that this magn...
Catalog: A13346
Size: 1 unit
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media
Custom Media: 2-MERCAPTOETHANOL
Catalog: A13558SA
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Other Instrument Accessories
The Luminex® Power Cord, USA (Catalog No. A13717) is provided by the Luminex Corporation (Catalog No. CN-P001-01). This power cord provides electrical power to a Luminex® instrument when it is connected to the appropriate electrical outlet. Please refer to a Luminex® instrument user manual for proper installation and use of this power cord with the appropriate Luminex® instrument. For research use only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: A13717
Size: 1 each
Category: Products & Services > Buffers & Chemicals > Immunoassay Buffers & Reagents > Luminex® Buffers & Reagents
xMAP® Sheath Fluid (20X Concentrate) gives you the power to store more in less space and enhance your inventory control. xMAP® Sheath Fluid (20X Concentrate) lets you make 20 L of 1X Luminex® Sheath Fluid for use as the delivery medium of the sample to the optics component of the Luminex® 100/200™ system and FLEXMAP 3D® system: xMAP® Sheath Fluid (20X Concentrate) contains: Sheath Concentrate (1 L) Preservative Concentrate This material is also available from Luminex (Cat. No. 40-75680). For research use only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: A13724
Size: 1 L
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media
Custom Media: MAGNESIUM CHLORIDE ANHYDROUS
Catalog: A13735IN
Size: 1 each
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Yeast Media
Gibco® YPD Broth is a ready-to-use liquid growth media used for yeast culture. Our filter-sterilized Gibco® YPD Broth can be used for nearly all research applications that require a liquid yeast culturing step such as protein expression, cloning, and expansion. • Gibco® microbiological media • Convenient ready-to-use liquid format • 1 L size available YPD Broth Recipe YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris ( P. pastoris ) and Saccharomyces cerevisae ( S. cerevisae ) yeast strains. Formulation per One Liter: 10 g Yeast extract 20 g Bac...
Catalog: A13745-01
Size: 1 L
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
GPX1 rabbit monoclonal antibody recognizes human glutathione peroxidase 1 (GPX1) in western blot and immunoprecipitation applications. Antibody Specifications Applications: Western blot, immunoprecipitation Species Reactivity: Human Clone ID: C8C4 Host Species and Isotype: Rabbit IgG
Catalog: A14011
Size: 100 µl
Category: Products & Services > Eukaryotic Cells > Stem Cells > Hematopoietic Stem Cells
StemPro® CD34+ cells are pooled Human Hematopoietic Progenitor cells (HPCs) derived from the umbilical cord blood of mixed donors. Along with 0.5×10 6 pooled donor cord blood-derived CD34+ cells, this kit includes StemPro®-34 SFM basal liquid medium and frozen StemPro®-34 Nutrient Supplement to facilitate the immediate culturing of the StemPro® CD34+ cells upon arrival or when recovering from cryopreservation storage. With the StemPro® CD34+ Cell Kit You Get: • ≥90% purity of the desired CD34+ cell population • Each vial of cells contains 0.5×10 6 CD34+ cells with full traceability • StemPro®-...
Catalog: A14059
Size: 0.5 x 10
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers
The Magnetic 96-Well Separator is designed for fast and simple removal of supernatant from samples bound to magnetic particles and may be used manually or as an integrated part of an automated workstation. The Magnetic 96-Well Separator is an ideal tool for use in singleplex and multiplex biological assays based on the Luminex® xMAP® Technology. These assays make use of Luminex® MagPlex™, or magnetic microspheres, and the MAGPIX®, Luminex® 200™ or FLEXMAP 3D® instruments. Note: It is recommended that the Magnetic 96-Well Separator be used with a standard 96-well flat-bottom plate such as Grein...
Catalog: A14179
Size: 1 each
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Modifying Enzymes
DNA End Repair Mix offers an easy, one step process for creating blunt ends in sheared, sonicated, or restriction-cut DNA and PCR fragments for efficient ligation into blunt end cloning vectors. DNA End Repair Mix allows you to: Generate blunt ends in DNA and PCR fragments (A-tail) Repair DNA with 5′ or 3′ overhangs in 30 minutes at 37°C Heat inactivate DNA End Repair Mix in 10 minutes at 75°C A Simple Way to Make Blunt Ends With DNA End Repair Mix, DNA with 5′ or 3′ overhangs is converted to 5′ phosphorylated blunt end DNA in 30 minutes at 37°C. Once DNA has been repaired, the enzymes in DNA ...
Catalog: A14321
Size: 20 reactions
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > 293 Media
Expi293™ Expression Medium is a chemically-defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density cultures of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression. • Supports growth of suspension 293 cultures to densities of over 1.6 x 10 7 cells/mL, with >95% cell viability • Transfection-compatible serum-free suspension culture medium allowing transfection efficiencies of greater than 70% using ExpiFectamine...
Catalog: A1435101
Size: 1000 ml
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
Alkaline Phosphatase Live Stain is a stem cell imaging product that allows users to differentially stain pluripotent stem cells (PSCs). The dye is a cell-permeable fluorescent substrate for alkaline phosphatase (AP) that is non-toxic to cells, diffusing out over the course of two hours. The Alkaline Phosphatase Live Stain consists of 50 µL of a 500X fluorescein-based dye in DMSO, sufficient for staining two 24-well plates, four 6 cm dishes or two 10 cm dishes. This Alkaline Phosphatase Live Stain allows you to: • Identify pluripotent stem cells with a strong signal above background • Stain PSC...
Catalog: A14353
Size: 50 µl
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Stem Cell Media
Essential 8™ Medium (published as E8) is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson (published as "E8") and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and is proven to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and has been shown to support iPSC growth for >50 passages without any signs of karyotypic a...
Catalog: A14666SA
Size: 500 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD90/Thy-1 (clone G7) R-PE (phycoerythrin) conjugate is a monoclonal rat antibody that recognizes the Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A). R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thereby affording exceptional quantum yields and molar extinction coefficients. The use of PE in antibody conjugates provides probes with greatly enhanced detectability. Antibody Specifications Description: CD90/Thy-1, Rat IgG 2c monoclonal Label: R-PE (phycoer...
Catalog: A14726
Size: 25 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD45, clone HI30, PerCP-Cy ® 5.5 conjugate is a mouse monoclonal antibody (mAb) that recognizes human CD45 molecule. The PerCP-Cy ® 5.5 tandem conjugate combines the peridinin-chlorophyll proteins (PerCP) complexes, found in nature, with the Cy ® 5.5 cyanine dye; thereby permitting simultaneous multicolor labeling and detection of multiple targets by utilizing a single excitation source - the 488 nm line of an argon-ion laser. Antibody Specifications Description: CD45, Mouse IgG 1 monoclonal Label: PerCP-Cy ® 5.5 tandem dye Validated Application: Flow Cytometry Species Reactivity: Human Ex...
Catalog: A14796
Size: 25 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > TR-FRET Primary Antibodies
LanthaScreen® terbium-labeled anti-GST Rabbit antibodies are used to indirectly label proteins tagged with GST. This can be advantageous when working with partially purified protein, or in cases in which direct labeling of the protein using amine-reactive Tb chelates negatively impacts protein activity.
Catalog: A15112
Size: 25 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > TR-FRET Primary Antibodies
LanthaScreen® terbium-labeled anti-GST Rabbit antibodies are used to indirectly label proteins tagged with GST. This can be advantageous when working with partially purified protein, or in cases in which direct labeling of the protein using amine-reactive Tb chelates negatively impacts protein activity.
Catalog: A15113
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > TR-FRET Primary Antibodies
LanthaScreen® terbium-labeled anti-GST Rabbit antibodies are used to indirectly label proteins tagged with GST. This can be advantageous when working with partially purified protein, or in cases in which direct labeling of the protein using amine-reactive Tb chelates negatively impacts protein activity.
Catalog: A15114
Size: 1 mg