Products from Avidin, Neutravidin®, Fluorescein Conjugate to Pichiapink™ Expression Strain Set
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: A-2662
Size: 1 mg
Size: 1 mg
Our horseradish peroxidase (HRP) conjugate of NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2664
Size: 1 mg
Size: 1 mg
Our NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2666
Size: 5 mg
Size: 5 mg
Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
Catalog: A-30005
Size: 1 mg
Size: 1 mg
The Alexa Fluor 405 goat anti-rabbit IgG is labeled with our bright, photostable, blue-fluorescent Alexa Fluor 405 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit.
Catalog: A-31556
Size: 0.5 ml
Size: 0.5 ml
The Violet Ratiometric Membrane Asymmetry Probe⁄Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry. The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis. The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 ...
Catalog: A-35137
Size: 1 kit
Size: 1 kit
Our 4-acetamido-4'-maleimidylstilbene-2,2'- disulfonic acid is a thiol-reactive reagent that is water soluble, with high polarity and membrane impermeability. This polysulfonated dye is useful for determining whether thiol-containing proteins and polypeptide chains are exposed at the extracellular or cytoplasmic membrane surface.
Catalog: A-485
Size: 25 mg
Size: 25 mg
In contrast to the other anti-fluorophore antibodies, which usually quench the fluorescence of the dye to which they bind, this anti-dansyl rabbit IgG antibody typically enhances the fluorescence of dansyl amides by greater than 10-fold. Binding of the anti-dansyl antibody also blue shifts the emission spectrum of the fluorophore in water from ~520 nm to ~450 nm. These properties, combined with the unusually high Stokes shift of the dansyl dye, make this antibody particularly useful for determining the topography of dansyl-labeled probes, including that of dansyl-labeled phospholipids in cell ...
Catalog: A-6398
Size: 0.5 ml
Size: 0.5 ml
The Attofluor cell chamber is a durable and practical coverslip holder designed for viewing live cell specimens on upright or inverted microscopes. Because the chamber is constructed from surgical stainless steel and is autoclavable, cells can be grown directly on coverslips within the unit. The O-ring seal design prevents samples from being contaminated by oil or leakage of media from the coverslips. The chamber accepts 25 mm round coverslips and mounts in a standard 35 mm diameter stage holder, and the 0.5 mm base dimension allows clearance for the objective when focusing. Spare O-rings for ...
Catalog: A-7816
Size: 3 each
Size: 3 each
The phycobiliprotein, allophycocyanin (APC) can be conjugated to proteins such as antibodies and other molecules to produce conjugates with excitation/emission maxima ~650/680 for use in flow cytometry applications. The Protein-Protein Crosslinking Kit (P6305) is optimized to crosslink APC to IgG antibodies.
Catalog: A-803
Size: 0.5 ml
Size: 0.5 ml
The Alexa Fluor 350 donkey anti - mouse IgG is labeled with our bright, photostable, blue-fluorescent Alexa Fluor 350 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. The donkey anti - mouse IgG antibodies show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins.
Catalog: A10035
Size: 0.5 ml
Size: 0.5 ml
One Shot® ccd B Survival™ 2 T1 Phage-Resistant (T1 R ) chemically competent E. coli are suitable for the propagation of plasmids containing the ccd B gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and super-coiled entry vectors. Transformation efficiency is >1 × 10 9 transformants/ µg DNA. The ccd B Survival™ 2 T1 R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1 R E. coli strain offers the following Features: • Resistance to the ccd B gene product • Resistance to T1 and T5 phage (the ton A/ fh...
Catalog: A10460
Size: 10 transformations
Size: 10 transformations
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryoti...
Catalog: A10462
Size: 10 µg
Size: 10 µg
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in...
Catalog: A10463
Size: 10 µg
Size: 10 µg
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors! The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in...
Catalog: A10464
Size: 10 µg
Size: 10 µg
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryotic systems • Ribosome bindi...
Catalog: A10465
Size: 10 µg
Size: 10 µg
Gibco® ACK (Ammonium-Chloride-Potassium) Lysing Buffer is suitable for use in the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library. cGMP Manufacturing and Quality System Gibco® ACK Lysing Buffer is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
Catalog: A10492-01
Size: 100 ml
Size: 100 ml
Affinity purified whole antibody goat anti-mouse IgG conjugated to 4% beaded, crosslinked agarose (Sepharose® CL-4B). To minimize cross-reactivity, this antibody has been absorbed against human IgG and human serum.
Catalog: A10516
Size: 2 ml
Size: 2 ml
Affinity purified whole antibody goat anti-mouse IgG conjugated to Cy5®. To minimize cross-reactivity, this antibody has been absorbed against human IgG and human serum.
Catalog: A10524
Size: 0.5 ml
Size: 0.5 ml
Affinity purified F(ab')2 fragment of goat anti-mouse IgG conjugated to R-phycoerythrin. To minimize cross-reactivity, this antibody has been absorbed against human IgG and serum.
Catalog: A10543
Size: 250 µl
Size: 250 µl
Affinity purified F(ab')2 gragment of goat anti-rat IgG conjugated to horseradish peroxidase. To minimize cross-reactivity, this antibody has been absorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Catalog: A10548
Size: 0.5 mg
Size: 0.5 mg
The Tali® Cell Cycle Kit includes a ready-to-use reagent that, when used with the Tali® Image-Based Cytometer, provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. This single-addition reagent is composed of propidium iodide (PI), RNase A, and Triton X-100. The Tali® Cell Cycle Kit offers a simple workflow: • The pre-mixed reagent can be stored at room temperature and used when your cells are ready • Simply fix your cells, add the reagent, incubate, and visualize • Cell cycle data can be analyzed on the Tali® instrument or exported as a ....
Catalog: A10798
Size: 50 assays
Size: 50 assays
The new Gibco® bottle has its own bottle weight for keeping order in the water bath! The bottle weight fits the new 500 mL and 1 L Gibco® bottles, keeping them upright in the water to further reduce your risk of contamination. Product Use For Research Use Only. Not for use in diagnostic procedures.
Catalog: A10988-01
Size: 1 each
Size: 1 each
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Catalog: A11105-01
Size: 100 ml
Size: 100 ml
Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger . Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the "pac" gene. Puromycin is widely used in cell biology as a selective agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2 – 5.0 µg/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/ml. Gibco® Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer. Life Technologies offer...
Catalog: A11138-02
Size: 20 ml
Size: 20 ml
The ViraPower™ HiPerform™ T-REx™ Gateway® Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄ TO⁄ V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscr...
Catalog: A11141
Size: 20 reactions
Size: 20 reactions
The ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit contains the MultiSite Gateway®-adapted ViraPower™ HiPerform™ promoterless lentiviral expression vector, pLenti6.4⁄R4R2⁄V5-DEST™ for easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any promoter of choice in dividing and non-dividing mammalian cells. The pLenti6.4⁄R4R2⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integras...
Catalog: A11146
Size: 20 reactions
Size: 20 reactions
The PichiaPink™ Secreted Protein Expression Kit is a new recombinant protein expression system based on the yeast Pichia pastoris . The PichiaPink™ Secreted Protein Expression Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant protein. The PichiaPink™ Secreted Protein Expression Kit comes with our new Pichia strains, the pPINK-HC and pPINK-LC vectors, and secretion signal sequences for production of your protein. • Get started with secreted protein production in Pichia pastoris . • Screen your Pichia transformants rapidly by ...
Catalog: A11151
Size: 1 kit
Size: 1 kit
The PichiaPink™ Secreted Vector Kit contains the pPINKα-HC vector for use with the PichiaPink™ Yeast Expression System. The pPINKα-HC vector is built around the ADE2 gene for complementing the complementing the ade2 deletion in the PichiaPink™ strains. The pPINKα-HC vector has the α-mating factor secretion signal sequence so that all proteins are secreted. You can order the PichiaPink™ Secreted Vector Kit to refill your PichiaPink™ Yeast Expression System Kit. The PichiaPink™ Secreted Vector Kit comes with: • pPINKα-HC vector (high-copy number) (20 µg) • 5’α-factor sequencing primer (20 µg) • ...
Catalog: A11153
Size: 1 kit
Size: 1 kit
The PichiaPink™ Expression Strain Set contains the 4 Pichia pastoris strains for use with the PichiaPink™ Expression System Kits. Each strain comes as a frozen stock in YPD media with 15% glycerol. All PichiaPink™ strains have the ade2 deletion. 3 strains have deletions of two protease genes, PEP4 and PRB1 . You can use the PichiaPink™ Expression Strain Set to refill your PichiaPink™ Yeast Expression System Kits. The PichiaPink™ Expression Strain Set includes the following Pichia strains: • PichiaPink™ Strain 1: ade2 • PichiaPink™ Strain 2: ade2 , pep4 • PichiaPink™ Strain 3: ade2 , prb1 • Pic...
Catalog: A11154
Size: 4 × 1ml
Size: 4 × 1ml
Products from Avidin, Neutravidin®, Fluorescein Conjugate to Pichiapink™ Expression Strain Set
View topsellers by:
Title |
Brand |
Price |
Popularity |
Favourites
Popular items by:
Popularity |
Our Choices |
All-Round Favourites |
Title
