Category: Products & Services > Instruments, Software, & Accessories > Imaging & Microscopy Accessories > Microscopy Accessories
The CultureWell cell culture systems provide an integrated set-up for preparing cultured cells for staining and imaging. Each system uses medical-grade silicone gaskets preassembled with standard optical-quality coverslips in cell culture plates. The entire system is provided sterile and ready to use.
Catalog: C-24760
Size: 1 set
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores
The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (K d ) of fluorescent Ca 2+ indicators using a fluorometer or quantitative imaging system. The Kd of an indicator can be calculated from a plot generated by scanning the excitation or emission of the indicator in the presence of eleven different Ca 2+ concentrations. Calcium Calibration Kit #1 contains 50 mL of 10 mM K 2 EGTA and 50 mL of 10 mM CaEGTA. Both solutions contain 100 mM KCl and 30 mM MOPS, pH 7.2 and are prepared in deionized water. These stock solutions can be blended to make buffers havin...
Catalog: C-3008MP
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > BioConjugates > Protein Conjugates
Molecular Probes® cholera toxin conjugates are made from a recombinant version of the B subunit only. This allows us to provide a very high-purity product that is completely free of the toxic A subunit. Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1 , making it a powerful tool for retrograde labeling of neurons. This tracer has been used in a variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall. When used in neuronal tracing applications, CT-B is typically introduc...
Catalog: C-34778
Size: 100 µg
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores
Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pK a of 4.8, this dye can be used as an acidic pH sensor.
Catalog: C-369
Size: 100 mg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Amine-Reactive Fluorophores, Biotins, Quantum Dots, & Other Labels
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: C-6164
Size: 10 mg
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides
Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis. ChromaTide® Labeled Nucleotides Specifications: • Ex/Em of dye: Texas Red®-12-dUTP (595/615 nm) • Length...
Catalog: C-7631
Size: 25 µl
Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains
The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.
Catalog: C10046
Size: 100 µl
Category: Products & Services > Competent Cells & Strains > Other Competent Cells & Strains
The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA str...
Catalog: C1010-10
Size: 10 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Counting & Flow Cytometer Accessories
Countess® Cell Counting Chamber Slides are designed for use with the Countess® Automated Cell Counter. Countess® slides are disposable. Each slide contains two cell counting chambers. This package comes with 1 box containing 50 slides and 2 x 1 mL vials of trypan blue (0.4%). Ready to Use For Your Convenience Each Countess® Cell Counting Chamber Slide comes presterilized and ready to use in an individual package. The Countess® slides are plastic and disposable. Cell counting occurs in a central location of the counting chamber. The volume counted is 0.4 µL, the same as counting four 1-mm 2 squ...
Catalog: C10228
Size: 1 box
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Apoptosis Kits
The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent® Caspase-3/7 Green Detection Reagent, as well as SYTOX® AADvanced™ Dead Cell Stain. • Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7 • Easy identification—clearly identify live, dead, and apoptotic cell populations • Quick analysis—washing and fixation is not required • Multicolor compatibility—combine with other dyes excitable by the 488 n...
Catalog: C10427
Size: 100 assays
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits
The Click-iT® HPG Alexa Fluor® 488 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 488 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 488 azide leads to a chemoselective ligation or "click" reaction between the...
Catalog: C10428
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits
The Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 594 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 594 azide leads to a chemoselective ligation or “click" reaction between the...
Catalog: C10429
Size: 1 kit
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Viability Kits
The CellROX® Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX® Deep Red Reagent, as well as SYTOX® Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS). The CellROX® Deep Red Flow Cytometry Assay Kit features: • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines,...
Catalog: C10491
Size: 100 assays
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Viability Kits
The CellROX® Green Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX® Green Reagent, as well as SYTOX® Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS). The CellROX® Green Flow Cytometry Assay Kit features: • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing ...
Catalog: C10492
Size: 100 assays
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Viability Kits
The CellROX® Orange Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX® Orange Reagent, as well as SYTOX® Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS). The CellROX® Orange Flow Cytometry Assay Kit features: • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowi...
Catalog: C10493
Size: 100 assays
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD8, clone 3B5, Pacific Green™ dye conjugate is a monoclonal mouse antibody that recognizes the CD8 antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD8, Mouse IgG2a monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity: Human ...
Catalog: C11205
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD45R (B220), clone RA3-B62, Pacific Green™ dye conjugate is a monoclonal rat antibody that recognizes the expressed product of the PTPRC gene. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD45R (B220), Rat IgG2a monoclonal Label: Pacific Green™ dye Validated Application:...
Catalog: C11206
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD4, clone RM4-5, Pacific Green™ dye conjugate is a monoclonal rat antibody that recognizes the mouse CD4 antigen and the expressed product of the CD4 gene. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD4, Rat IgG2a monoclonal Label: Pacific Green™ dye Validated Applicat...
Catalog: C11207
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD45, clone HI30, Pacific Green™ dye conjugate is a monoclonal mouse antibody that is reactive to the human CD45 antigen and recognizes the expressed product of the PTPRC gene. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD45, Mouse IgG1 monoclonal Label: Pacific Green™ ...
Catalog: C11208
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD45RA, clone MEM-56, Pacific Green™ conjugate is a monoclonal mouse antibody that recognizes the CD45RA antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD45RA, Mouse IgG2b monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity...
Catalog: C11209
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD19, clone SJ25-C1, Pacific Green™ dye conjugate is a monoclonal mouse antibody that recognizes the CD19 antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD19, Mouse IgG1 monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity: ...
Catalog: C11210
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD14, clone TüK4, Pacific Green™ dye conjugate is a monoclonal mouse antibody that recognizes the CD14 antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD14, Mouse IgG2a monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity: Hu...
Catalog: C11211
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD3, clone UCHT1, Pacific Green™ dye conjugate is a monoclonal antibody that is reactive to the human CD3 antigen and recognizes the expressed product of the CD3D gene. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD3, Mouse IgG1 monoclonal Label: Pacific Green™ dye Valid...
Catalog: C11213
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD8A, clone 5H10, Pacific Green™ dye conjugate is a monoclonal mouse antibody that is reactive to the mouse CD8α (Ly-2) antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD8α, Rat IgG2b monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species...
Catalog: C11214
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD4, clone S3.5, Pacific Green™ conjugate is a monoclonal mouse antibody that recognizes the CD4 antigen also known as T4 and L3T4. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD4, Mouse IgG2a monoclonal Label: Pacific Green™ Validated Application: Flow Cytometry Species...
Catalog: C11215
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD3, clone S4.1, Pacific Green™ dye conjugate is a monoclonal antibody that is reactive to the human CD3 antigen.The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD3, Mouse IgG2a monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity: Hu...
Catalog: C11216
Size: 125 µl
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2110
Size: 5 mg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2925
Size: 1 mg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2927
Size: 1 mg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C34551
Size: 20 × 50 µg