Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > DNA & RNA Storage Reagents
RNAlater®-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater®-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA. • Process previously frozen tissues like freshly harvested samples • Thawing tissues in RNAlater®-ICE protects RNA from degradation • No more tissue pulverizing with mortar and pestle and awkward transf...
Catalog: 4427575
Size: 10 bottles
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits
TaqMan® Universal Master Mix II is a convenient 2X mix for use in all real-time application including microRNA, gene expression and genotyping. TaqMan® Universal Master Mix II is a convenient 2X mix that includes: -AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure), a highly purified thermostable DNA polymerase, that is hot start-enabled for convenient reaction setup at room temperature -Available in two formats: with and without uracil-DNA glycosylase (UDG) and dUTP⁄dTTP mix to minimize carryover PCR contamination -Optimized salt, dNTP, and buffer concentrations for reliable performance -Passive ...
Catalog: 4440043
Size: 40 reactions
Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins
This is the User Guide Manual for the AmpFLSTR® Identifiler® Plus PCR Amplification Kit. Note: For Forensic or Paternity Use Only.
Catalog: 4440211
Size: 1 manual
Category: Products & Services > Buffers & Chemicals > Denaturing Agents
The highly deionized (Hi-Di) Formamide is used to resuspend samples before electrokinetic injection in capillary electrophoresis systems. The smaller configuration (5 ml) minimizes the number of freeze⁄thaw cycles compared to the larger 25ml bottle and may be a better fit when running a lower number of samples. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4440753
Size: 4 × 5 ml
Category: Products & Services > Oligos, Primers, Probes & Nucleotides
Custom Plus TaqMan® RNA Assay, MED
Catalog: 4441117
Size: 750 µl
Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > MicroRNA Arrays
The TaqMan® Array Human MicroRNA B Card v3.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 377 human microRNAs in as little as 5 hours. Three TaqMan MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan® MicroRNA Assay not related to human is included as a negative control. Details on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Human Pool B v3.0 is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Human Pool B v3.0 for situatio...
Catalog: 4444910
Size: 4 pack
Category: Products & Services > Kits & Assays > Impurity & Contamination Detection Kits > Mycoplasma Detection Kits
The Discriminatory Positive⁄Extraction Control is a large plasmid containing a Mycoplasma DNA sequence. This control was designed to behave like Mycoplasma DNA in both the sample preparation and detection portions of the assay. Additionally, the DNA sequence has been modified so that the amplicon generated from this control has a melting temperature (Tm) of approximately 84°C, which is outside the range of amplicons generated from Mycoplasma with this assay. Thus, the Tm can be used to discriminate between a positive test result from Mycoplasma and the control DNA. This novel control enables r...
Catalog: 4445000
Size: 1 kit
Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
The Protein Quant Sample Lysis Kit contains the reagents required for the preparation of target and control cell lysates for use with TaqMan® Protein Assay experiments. It contains a one-step lysis solution for preparing crude cell lysates from cultured cells, and the lysates are compatible with cell counting as well as total protein quant measurements such as a microBCA assay. The Protein Quant Sample Lysis Kit contains a buffered nonionic detergent solution for the gentle lysis of adherent and non-adherent cultured cells under non-denaturing conditions
Catalog: 4448536
Size: 25 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis. • Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit • Simple magnetic bead workflow—no spin columns • Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors Maximize Total ...
Catalog: 4451894
Size: 96 preps
Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Genetic & Chromosome Abnormality Detection Kits
The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...
Catalog: 4452136
Size: 1 kit
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Real-Time PCR Instruments
The ViiA™ 7 Real-Time PCR System combines all of the qPCR features you want in a single high performance instrument, so that you can optimize your research productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, the ViiA™ 7 System offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or fast-cycling 384- or 96-well plates and TaqMan® Array Micro Fluidic Cards and Reagents makes high-productivity real-time PCR accessible to any size l...
Catalog: 4453535
Size: 1 instrument
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Real-Time PCR Instruments
The ViiA™ 7 Real-Time PCR System combines all of the qPCR features you want in a single high performance instrument, so that you can optimize your research productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, the ViiA™ 7 System offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or fast-cycling 384- or 96-well plates and TaqMan® Array Micro Fluidic Cards and Reagents makes high-productivity real-time PCR accessible to any size l...
Catalog: 4453536
Size: 1 instrument
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Real-Time PCR Instruments
The ViiA™ 7 Real-Time PCR System combines all of the qPCR features you want in a single high performance instrument, so that you can optimize your research productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, the ViiA™ 7 System offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or fast-cycling 384- or 96-well plates and TaqMan® Array Micro Fluidic Cards and Reagents makes high-productivity real-time PCR accessible to any size l...
Catalog: 4453537
Size: 1 instrument
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Real-Time PCR Instrument Accessories
This kit allows the ViiA™ 7 Real-Time PCR System to operate using a Fast 96-well block. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4453544
Size: 1 upgrade
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Real-Time PCR Instrument Accessories
This kit allows the ViiA™ 7 Real-Time PCR System to operate using a TaqMan® Array Block. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4453546
Size: 1 upgrade
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Real-Time PCR Instrument Accessories
This kit contains the necessary accessories to process 10 TaqMan OpenArray® Real-Time PCR plates. TaqMan® OpenArray® Real-Time PCR Plates deliver world-class TaqMan® Assays in a more flexible, high-throughput format. Five customizable plate formats allow you to select from over 61,000 inventoried TaqMan® Gene Expression Assays, which are pre-loaded onto the OpenArray® Plates. View the available formats (PDF). The OpenArray® Real-Time PCR System enables over 8,000 real-time PCR assays to be run simultaneously to generate 32,000+ data points per day. As part of our greener product alternatives w...
Catalog: 4453993
Size: 1 kit
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII is a single kit for RNA library construction of either whole transcriptome or small RNA (such as miRNA), for use in downstream next generation sequencing analysis. Designed as a complete solution with a common workflow, the kit optimally combines all necessary reagents for discovery of coding RNA, non-coding RNA, alternative splicing, small RNAs and isoforms, while preserving strand orientation. The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII platform is based on the same technology used in ...
Catalog: 4454073
Size: 1 kit
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls
Silencer ® Select MALAT1 Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. 5 nmol is provided. • Premium-quality siRNA, highly purified and ready to use • Functionally tested in several common cell lines • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs Silencer ® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, poten...
Catalog: 4455877
Size: 5 nmol
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
Variation in RNA expression data can be attributed to a variety of factors including the quality of the starting material, the level of cellularity and RNA yield, the platform employed, and the person performing the experiment. To control for these sources of variability, a common set of external RNA controls has been developed by the External RNA Controls Consortium (ERCC), an ad-hoc group of academic, private, and public organizations hosted by the National Institute of Standards and Technology (NIST). The controls consist of a set of unlabeled, polyadenylated transcripts designed to be adde...
Catalog: 4456740
Size: 1 kit
Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Other Enzyme Assay Kits
The NA-Fluor™ Influenza Neuraminidase Assay Kit provides validated reagents and a standardized protocol for conducting neuraminidase enzyme assays, including neuraminidase inhibitor (NI) susceptibility screening using a fluorescent MUNANA substrate. Key product features: • Efficient design—neuraminidase assay in one complete kit • Optimized formulations—assay reagents have been optimized for maximum performance based on NISN protocols • Robust application—functional detection of NI-resistant virus in mixed viral populations • Easy of use & flexible screening—stable fluorescent signal enables b...
Catalog: 4457091
Size: 960 assays
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Negative Controls
Ambion® In Vivo siRNA Controls • Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts • Positive Control siRNAs functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability • For use in human, mouse, and rat cells What are Ambion® In Vivo siRNAs? Ambion® In Vivo siRNAs are designed using the proven Silencer ® Select algorithm and incorpora...
Catalog: 4457287
Size: 5 nmol
Category: Products & Services > Kits & Assays > Impurity & Contamination Detection Kits > Host Cell DNA & Protein Impurity Quantitation Kits
The resDNASEQ® NS0 Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of NS0 cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA. Thi...
Catalog: 4458441
Size: 100 reactions
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls
Ambion® In Vivo siRNA Controls • Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts • Positive Control siRNAs functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability • For use in human, mouse, and rat cells What are Ambion® In Vivo siRNAs? Ambion® In Vivo siRNAs are designed using the proven Silencer ® Select algorithm and incorpora...
Catalog: 4459407
Size: 50 nmol
Category: Products & Services > Kits & Assays > Impurity & Contamination Detection Kits > Host Cell DNA & Protein Impurity Quantitation Kits
The resDNASEQ® NS0 Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of NS0 cell DNA, typically in less than 4 hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high m...
Catalog: 4460364
Size: 100 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sequencing & Genetic Analyzer Instruments
The 3500 Dx Genetic Analyzer CS2 is the latest addition to our family of Applied Biosystems® genetic analyzers based on capillary electrophoresis. The 3500 Dx Genetic Analyzer CS2 is an IVD-labeled instrument, making it the first Sanger sequencing platform cleared for sequencing analysis of DNA. Confidence in Your Results • The 3500 Dx Genetic Analyzer CS2 is an IVD-labeled platform for Sequencing-Based Typing using SeCore® HLA typing kits. Sanger sequencing is known as the “gold-standard” for its accuracy, reliability, and ease of use. It is also used for confirmatory sequencing by clinical l...
Catalog: 4461447
Size: 1 system
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sequencing & Genetic Analyzer Instruments
The 3500 Dx Genetic Analyzer CS2 is the latest addition to our family of Applied Biosystems® genetic analyzers based on capillary electrophoresis. The 3500 Dx Genetic Analyzer CS2 is an IVD-labeled instrument, making it the first Sanger sequencing platform cleared for sequencing analysis of DNA. Confidence in Your Results • The 3500 Dx Genetic Analyzer CS2 is an IVD-labeled platform for Sequencing-Based Typing using SeCore® HLA typing kits. Sanger sequencing is known as the “gold-standard” for its accuracy, reliability, and ease of use. It is also used for confirmatory sequencing by clinical l...
Catalog: 4461450
Size: 1 system
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories
This is a pump cleaning kit for the 3500 Series platform. It is highly recommended that a field service engineer perform any necessary pump cleaning.
Catalog: 4461875
Size: 1 each
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards
This kit includes all of the consumables and accessories needed to perform an instrument qualification—install, operational, or/and performance. This kit is specific for the 3500 Dx CS2 instrument and comes with a 50-cm 8-capillary array, POP-6™ polymer, and Big Dye® Terminator v1.1 sequencing standard.
Catalog: 4462042
Size: 1 kit
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards
This kit includes all of the consumables and accessories needed to perform an instrument qualification—install, operational, or/and performance. This kit is specific for the 3500xL Dx CS2 instrument and comes with a 50-cm 24-capillary array, POP-6™ polymer, and Big Dye® Terminator v1.1 sequencing standard.
Catalog: 4462043
Size: 1 kit
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards
This BigDye® Terminator v1.1 sequencing standard consists of four tubes, each of which contains DNA of a known 1,200 base pair sequence prepared with BigDye® Terminator v1.1. Sufficient DNA is included to perform one spectral calibration⁄performance check and⁄or one control sequencing run on an Applied Biosystems® 3500 Dx Series genetic analyzer. The DNA preparation has been lyophilized to maximize stability.
Catalog: 4462113
Size: 4 tubes