Category: Products & Services > Buffers & Chemicals > General Chemicals > Oligo Synthesis Reagents
Contains 50 mg TET™ Dye Phosphoramidite. TET™ Dye Phosphoramidite is used for the 5’ attachment of TET™ dye during oligonucleotide synthesis. The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesis • Life Technologies offers a range of specialty phosphoramites and linkers...
Catalog: 403171
Size: 50 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
• Reactivity -Human • Applications -Flow Cytometry, Functional Studies, Immunohistochemistry (Frozen)*, Immunoprecipitation*, Inhibition* • Host, Isotype -Mouse, IgG2a-κ • Clonality, Clone -mAb, 7G2 *Application tested by outside source. Ms anti-CD73; Unconjugated Monoclonal antibody specific to Human CD73 . This antibody is validated for use in Flow Cytometry, Functional Studies, Inhibition of E-cadherin-dependent cell-to-cell contact, Immunohistochemistry, Immunoprecipitation. Anti-CD73 recognizes the expressed product of the NT5E gene.
Catalog: 41-0200
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Rb anti-KLF4; Unconjugated Polyclonal antibody specific to Human, (Mouse, Rat, Sw, Chicken, Bovine, Cp) KLF4 . This antibody is validated for use in Western Blot. Anti-KLF4 recognizes the expressed product of the KLF4 gene, also known as Klf4, Klf4.
Catalog: 42-4100
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
Reactivity: FITC-SA will react with biotin.
Catalog: 43-4311
Size: 1 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
Reactivity: CY3-SA reacts with biotin.
Catalog: 43-4315
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin
Reactivity: CY5-SA reacts with biotin.
Catalog: 43-4316
Size: 1 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Avidin
Reactivity: FITC-Avidin reacts with biotin.
Catalog: 43-4411
Size: 5 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Avidin
Reactivity: HRP-Avidin reacts with biotin. Applications: Immunohistochemistry, ELISA
Catalog: 43-4423
Size: 5 mg
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > dNTPs
GeneAmp® dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. GeneAmp® dNTPs with dUTP can be used to generate dU-containing PCR products in a carry-over prevention strategy in conjunction with AmpErase ® Uracil N-glycosylase (UNG). dNTPs have high purity, making them ideal for use in PCR reactions or other applications. dNTPs with dUTP are optimized for use in carry-over prevention. Guaranteed for PCR performance when used with GeneAmp® PCR core reagents and GeneAmp® instrument systems. Premixed dNTP blends are available in various conce...
Catalog: 4303443
Size: 12 x 1 ml
Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits
This Applied Biosystems® kit provides the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, and oligo (dT) 16 , reverse primers are included for cDNA synthesis flexibility. Supplied as 2000 reactions in 10 tubes (200 reactions/tube). • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions TaqMan® Reverse Transcription Reagents provide all...
Catalog: 4304134
Size: 2000 reactions
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits
Applied Biosystems® TaqMan® Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® real-time PCR instru...
Catalog: 4304437
Size: 200 reactions
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits
This kit contains components needed to perform up to 1000 assays using TaqMan® probes. The kit employs AmpliTaq Gold® DNA polymerase for enhanced performance, 10X TaqMan Buffer A, and magnesium chloride. The components are provided in bulk and enable flexibility in assay setup. Separate Components Provide Flexibility in Assay Set-Up AmpliTaq Gold® DNA polymerase increses yield and gives a more robust 5' nuclease assay than AmpliTaq® DNA polymerase. Passive Reference I in the 10X TaqMan® Buffer A normalizes signal in all TaqMan® Assay reactions and corrects inter-well signal variation caused by...
Catalog: 4304441
Size: 1000 reactions
Category: Products & Services > Instruments, Software, & Accessories
Catalog: 4305936
Size: 500 tubes
Category: Products & Services > Buffers & Chemicals > Molecular Biology Enzyme Buffers > PCR Enzyme Buffers
Applied Biosystems® GeneAmp®10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold® DNA Polymerase, resulting in highly specific, robust PCR amplification. When diluted 1:10, provides the optimal pH and ionic strength for PCR activation and amplification with AmpliTaq Gold® DNA Polymerase, ensuring maximum performance Available in several convenient sizes GeneAmp® 10X PCR Buffer II is both magnesium ion free and gelatin free. A separate MgCl 2 solution is provided. When used together, GeneAmp® 10X PCR Buffer II and MgCl 2 solution offer more versatility in...
Catalog: 4306898
Size: 6 × 1.5 ml
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® TaqMan® Rodent GAPDH Control Reagents provide the components for using rodent GAPDH as a normalization control in applications with rat, mouse, and Chinese hamster cells. • Pre-designed primers and TaqMan® probe eliminate assay design • Rapid assay development guidelines can minimize optimization time • Separate components provide flexibility in assay set-up Comprehensive Guidelines Included Applied Biosystems® has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems® Real-Time PCR reagents and instrumentation. These guidelines are...
Catalog: 4308313
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
Applied Biosystems® TaqMan® Ribosomal RNA Control Reagents are designed to detect the 18S ribosomal RNA (rRNA) gene. The TaqMan® probe and primer sequences were designed following Rapid Assay Development Guidelines. The TaqMan® probe and primer sequences are conserved among a diverse group of eukaryotes including human, rat, mouse, Xenopus, Saccharomyces, Giardia, and Arabidopsis. Ribosomal RNA levels provide an endogenous control for PCR quantitation studies. • Pre-designed primers and TaqMan® probe eliminate assay design • Rapid Assay Development Guidelines can minimize optimization time • S...
Catalog: 4308329
Size: 1000 reactions
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits
Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial. • Premixed components stored at 2–8°C significantly reduce assay setup time • SYBR® Green I dye detects double-stranded DNA, so specific probes are not required • AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance • dUTP significant...
Catalog: 4309155
Size: 200 reactions
Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > PCR Plates
The Applied Biosystems® MicroAmp® Optical 384-Well Reaction Plate is designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a 384-well format, is designed to work with Applied Biosystems® 384-well Real-Time PCR Systems and Thermal Cyclers. • Frosted to minimize interfering fluorescence from wells of cycling block • Screened to eliminate auto-fluorescent plates • Unique design provides a barrier to ambient air to help insure well-to-well temperature uniformity • Each reaction ...
Catalog: 4309849
Size: 50 plates
Category: Products & Services > Instruments, Software, & Accessories > Instruments > PCR Machines (Thermal Cyclers)
The Applied Biosystems® GeneAmp® PCR System 9700 is specifically designed for reliable PCR. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The 0.5 mL GeneAmp® PCR System 9700 is designed for use with 0.5 mL reaction tubes for all of your large volume PCR applications. • Request a Demo Key Highlights: • Heated lid for oil-free operation in a 60-well, 0.5 mL format • Interchangeable sample blocks for increased flexibility • Graphical user interface for easy, intuitive programming • Designed for fast thermal response and exceptional un...
Catalog: 4310899
Size: 1 instrument
Category: Products & Services > Instruments, Software, & Accessories > Software > Sequencing & Genetic Analysis Software
This product can only be used to upgrade Sequencing Analysis Software v5.3.1 installed on standalone computers, computers separate to the instrument data collection system. Please talk with your representative to upgrade the data collection system and secondary analysis software running on the data collection computer. Sequencing Analysis Software enables you to basecall, trim, display, edit, and print data from our entire line of capillary DNA sequencing instruments for data analysis and quality control. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4310991
Size: 1 CD
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Reverse Transcriptase
Applied Biosystems® MultiScribe™ Reverse Transcriptase is a recombinant Moloney murine leukemia virus (rMoMuLV) reverse transcriptase. MultiScribe™ Reverse Transcriptase is similar to MuLV reverse transcriptase but differs in its recommended usage. MultiScribe™ Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. 5000U supplied in 100 µl (50U/ µl). • Compatible with one-step and two-step RT-PCR reactions, which provides flexibility in assay set-up • Individual...
Catalog: 4311235
Size: 100 µl
Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Adhesive Film & Other Plate-Related Accessories
Applied Biosystems® MicroAmp® Optical Film Compression Pads are designed to support a proper thermal seal between the thermal cycler and reaction plates when using adhesive films. Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4312639
Size: 5 each
Category: Products & Services > Oligos, Primers, Probes & Nucleotides
TaqMan® MGB Probe
Catalog: 4316033
Size: 20,000 pmol
Category: Products & Services > Oligos, Primers, Probes & Nucleotides
TaqMan® MGB Probe
Catalog: 4316034
Size: 6,000 pmol
Category: Products & Services > Instruments, Software, & Accessories > Capillaries & Polymers > Polymers
POP-6™ is a DNA sequencing and fragment analysis reagent and consumable. It is a separation matrix for performing fragment analysis and sequencing on the 3100 Genetic Analyzer and 3100-Avant™ Genetic Analyzer. The POP-6™ Polymer is ideally suited for standard and rapid sequencing. Many Choices For the ABI PRISM® 310 and 3100 Genetic Analyzers, POP-4® polymers are designed for microsatellite and other genotyping applications, while POP-6™ polymers are the choice for standard and rapid sequencing. For the ABI PRISM® 3700 DNA Analyzer, POP-5™ polymer is the separation matrix of choice for high-th...
Catalog: 4316357
Size: 7000 µl
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The RNase P gene is a single-copy gene that encodes the RNA moiety for the RNase P enzyme. Applied Biosystems® TaqMan® RNase P Control Reagents were designed with limiting primer concentrations to be used as the endogenous reference in multiplex reactions. Multiplex PCR is the use of more than one probe and primer pair in the same tube. This kit is designed to perform a 5' nuclease assay with TaqMan® Universal PCR Master Mix using the provided human genomic DNA as template. • Pre-designed primers and TaqMan® probe eliminate assay design • Easy-to-use primer and TaqMan® probe mix minimizes assa...
Catalog: 4316844
Size: 1000 reactions
Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins
This is the Site Prep and Safety Guide for the 7900HT Sequence Detection System. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4317595
Size: 1 unit
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Catalog: 4318930
Size: 1 kit
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Eukaryotic 18S rRNA Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan Gene Expression Assays, Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: 18S RefSeq: X03205.1 Probe Exo...
Catalog: 4319413E
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > Fragment Analysis Size Standards
The GeneScan™ 500 LIZ® Size Standard is a fifth dye-labeled size standard for the reproducible sizing of fragment analysis data. GeneScan™ 500 LIZ® Size Standard is designed for sizing DNA fragments in the 35-500 nucleotides range and provides 16 single-stranded labeled fragment of: 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490 and 500 nucleotides. The sizing curve generated from these fragments make the GeneScan™ 500 LIZ® Size Standard ideal for a variety of fragment analysis applications such as Microsatellites, Fragment Length Polymorphisms and Relative Fluorescent ...
Catalog: 4322682
Size: 800 reactions