Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin
Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing This Trypsin is modified as follows: With • EDTA • Phenol Red
Catalog: 25300-120
Size: 20 x 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum > Specialty Fetal Bovine Serum
Sera Category: Specialty Origin: United States. Endotoxin level: 50 EU/ml (Specification is checked and recorded; levels routinely Hemoglobin level: 25 mg/dl.
Catalog: 26400-036
Size: 100 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin
Gibco® Trypsin 1:250 powder is an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging, and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing Quality Testing Gibco® Trypsin 1:250 is tested for endotoxin, mycoplasma, bacterial, fungal and viral contamination. Other quality tests include multiple activity assays, ash analysis and mois...
Catalog: 27250-018
Size: 100 g
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This monoclonal antibody reacts with the extracellular domain of epidermal growth factor receptor (170 kDa). Clone 31G7 is useful in detecting the majority of squamous cell carcinomas that arise from both squamous epithelium and metaplastic squamous epithelium. Positive tissue: Breast carcinoma. Clone/PAD: 31G7 Immunogen: EGFr derived from A-431 cells Specificity: EGFr. No cross-reactivity observed with c-erbB-2. Reactivity: Human Applications: Immunohistochemistry (FFPE) Pretreatment: Epitope Retrieval: Not required , Enzyme Digestion: Required (Digest-All™ 3/Pepsin) Expected Staining Pattern...
Catalog: 28-0005
Size: 2 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Enoyl-[acyl-carrier-protein] Reductase NADH-dependent (FABI), a recombinant bacterial protein, is supplied in frozen format and is quite suitable for use in protein structure analyses. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the E.coli FABI (NP_287908.1) (Gly 2-Lys 262) that is fused to a polyhistidine tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 0.5 M NaCl, 10% glycerol, pH 7....
Catalog: 30001-E07E-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Enoyl-[acyl-carrier-protein] Reductase NADH-dependent (FABI), a recombinant bacterial protein, is supplied in frozen format and is quite suitable for use in protein structure analyses. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the E.coli FABI (NP_287908.1) (Gly 2-Lys 262) that is fused to a polyhistidine tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 0.5 M NaCl, 10% glycerol, pH 7....
Catalog: 30001-E07E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
Tfi DNA Polymerase is a new non- Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees. Like Taq , Tfi has 5'→3' exonuclease and 5'→3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness (Figure 1, A and B). Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4 kb in length. In addition, Tfi is free of licensing restrictions that accompany Taq polymerase. Tfi can be considered for use as a co...
Catalog: 30342-052
Size: 5000 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sequencing & Genetic Analyzer Instruments
The 310 Genetic Analyzer is an automated single-capillary genetic analyzer designed for a wide range of sequencing and fragment analysis applications. • Perform comparative sequencing, linkage analysis, STR analysis, SNP detection, discovery and validation, mutation detection, and many other applications. • Eliminate gel preparation. • Set up runs in minutes. • Automate your workflow to minimize hands-on time and costs. • Detect five dyes simultaneously to increase throughput. Versatile, Affordable, Easy-to-Use, and Almost Hands-Free The 310 Genetic Analyzer – the ideal alternative to gel-base...
Catalog: 310-00-100-120WR
Size: 1 instrument
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 31053-028
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > 2-Mercaptoethanol
Gibco® 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. Mercaptoethanol is not stable in solution so most protocols require daily supplementation. Gibco® 2-Mercaptoethanol is made at 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). Product Use For Research Use Only. Not for use in diagnostic procedures. cGMP Manufacturing and Quality System Gibco® 2-Mercaptoethanol is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the...
Catalog: 31350-010
Size: 20 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 31985-070
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 31985-088
Size: 10 × 500 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This purified Mouse anti-GluR2 Monoclonal Antibody is specific to human, rat, monkey, and mouse GluR2. In addition to its recognized role as a calcium-independent cell adhesion molecule, mouse anti-GluR2 monoclonal antibody recognizes the ~96kDa GluR2 protein. Validated applications for mouse anti-GluR2 monoclonal antibody are immunostaining, ELISA and Western blotting. Antibody Attributes: • Applications: Validated applications for this mouse anti-GluR2 monoclonal antibody are immunostaining, ELISA and Western blotting. • Host Species and Isotype: The host species and isotype of this GluR2 mo...
Catalog: 32-0300
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Mouse anti-Claudin-15; Unconjugated Monoclonal antibody specific to Human, Mouse, Rat Claudin-15 . This antibody is validated for use in Western Blot, Immunohistochemistry, Immunoassay (ELISA), Immunocytochemistry/ Immunofluorescence. Anti-Claudin-15 recognizes the expressed product of the CLDN15 gene.
Catalog: 32-9800
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This purified E-Cadherin Monoclonal Antibody is specific to human E-Cadherin. The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells. Mouse anti- E-Cadherin monoclonal antibody can be used in applications such as western blotting, immunohistochemistry, immunocytochemistry/immunofluorescence, and immunoprecipitation. This anti- E-Cadherin monoclonal antibody recognizes the product of the CDH1 gene. Antibody Specifications: • Applications: Validated applications for this E-Cadherin antibody are western blotting, immunohistochemistry, and immunoprec...
Catalog: 33-4000
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Mouse anti-Maltose Binding Protein; Unconjugated Monoclonal antibody specific to Multiple Maltose Binding Protein. This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-Maltose Binding Protein recognizes the expressed product of the MBP gene.
Catalog: 33-5100
Size: 100 µg
Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards
AB-Human Serum (heat-inactivated)
Catalog: 34005-100
Size: 100 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Mouse anti-phospho-Tau (Thr231); Unconjugated Monoclonal antibody specific to Human Tau [pT231]. This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-Tau [pT231] recognizes the expressed product of the MAPT gene.
Catalog: 35-5200
Size: 50 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This purified mouse anti-NY-ESO-1 monoclonal antibody recognizes human NY-ESO-1. NY-ESO-1 is a member of the cancer/testis (CT) family of antigens, which are expressed in a range of malignant neoplasmas such as melanoma and carcinomas of the breast, ovary and lung, but not in normal tissue except in testis. NY-ESO-1 is a classic CT antigen discovered during a SEREX analysis of esophageal cancer. It is a 22–23 kDa hydrophobic protein. Validated applications for anti-NY-ESO-1 are western blotting and immunohistochemistry (paraffin). Antibody Specifications: • Applications: Validated applications...
Catalog: 35-6200
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Epitope Tag & Reporter Specific Antibodies
This mouse anti-Luciferase monoclonal antibody is specific to mouse Luciferase protein. Luciferase is a 61 kD enzyme that catalyzes the reaction of luciferin to generate light after its activation by ATP and oxidative decarboxylation Validated applications for Luciferase monoclonal antibody are Western blotting, immunofluorescence, and immunohistochemistry (frozen tissue). Applications: validated Western blotting, immunofluorescence, and immunohistochemistry (frozen tissue) Host species and isotype: mouse IgG1 Clone ID of monoclonal antibody (mAb): CS 17 Reactivity: purified Luciferase in mous...
Catalog: 35-6700
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Rabbit anti-M-Cadherin; Unconjugated Polyclonal antibody specific to Mouse M-Cadherin. This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-M-Cadherin recognizes the expressed product of the Cdh15 gene.
Catalog: 36-2100
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Mouse anti-RKIP (C-term); Unconjugated Monoclonal antibody specific to Human, Mouse RKIP (C-term) . This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-RKIP (C-term) recognizes the expressed product of the PEBP1 gene, also known as Pebp1.
Catalog: 37-2100
Size: 100 µg
Category: Products & Services > Beads & Microspheres > Dynabeads® Surface-Activated
An excellent tool for highly predictable and consistent extraction and isolation of nucleic acids from biological samples, following a simple magnetic separation protocol. Please note that this is a stand-alone product containing only Dynabeads® in a storage buffer. Our products Dynabeads® SILANE genomic DNA (Cat.no. 370-12D) or Dynabeads® SILANE viral NA (Cat.no. 370-11D) include the appropriate buffers and protocols you need to extract genomic DNA or viral nucleic acids respectively. Magnetic particles from alternative suppliers often have a random size range distribution, surface area and b...
Catalog: 37002D
Size: 5 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Ms anti-Nix; Unconjugated Monoclonal antibody specific to Human, Mouse, (Rat) Nix. This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-Nix recognizes the expressed product of the BNIP3L gene, also known as Bnip3l, Bnip3l.
Catalog: 39-3300
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This rabbit anti-Cdc4 polyclonal antibody is specific to human Cdc4 protein and expected to react with mouse (not tested). Cdc4 polyclonal antibody recognizes the expressed product of the Fbxw7 gene, also known as FBXW7. Cdc4 is an inhibitor of Notch signaling that targets Notch for ubiquitin-mediated degradation after a nuclear phosphorylation event. Validated applications for Cdc4 polyclonal antibody are Western blotting and immunoprecipitation. Applications: validated for Western blot and immunoprecipitation Host species and isotype: rabbit IgG Polyclonal Antibody Designation: ZMD.432 React...
Catalog: 40-1500
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Viral & Microbial Proteins
Hemagglutinin (HA), recombinant viral protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 539) of the influenza hemagglutinin (A/Duck/Hong Kong/p46/97 (H5N1)) (HA1+HA2, uncleaved), cleavage site mutated (RRRKKR-TETR) fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , 887 mM NaCl, 2...
Catalog: 40001-V08H-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Viral & Microbial Proteins
Hemagglutinin (HA), recombinant viral protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 539) of the influenza hemagglutinin (A/Duck/Hong Kong/p46/97 (H5N1)) (HA1+HA2, uncleaved), cleavage site mutated (RRRKKR-TETR) fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , 887 mM NaCl, 2...
Catalog: 40001-V08H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Viral & Microbial Proteins
Hemagglutinin (HA), recombinant viral protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 539) of the influenza hemagglutinin (A/Duck/Hong Kong/p46/97 (H5N1)) (HA1+HA2, uncleaved), cleavage site mutated (RRRKKR-TETR) fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , 887 mM NaCl, 2...
Catalog: 40001-V08H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Viral & Microbial Proteins
Hemagglutinin (HA), recombinant viral protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 539) of the influenza hemagglutinin (A/Duck/Hong Kong/p46/97 (H5N1)) (HA1+HA2, uncleaved), cleavage site mutated (RRRKKR-TETR) fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , 887 mM NaCl, 2...
Catalog: 40001-V08H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Viral & Microbial Proteins
Hemagglutinin subunit 1 (HA1), recombinant viral protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the N-terminal segment (Met 1-Glu 348) of the influenza hemagglutinin (A/Duck/Hong Kong/p46/97 (H5N1)), termed as HA1, fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5%...
Catalog: 40001-V08H1-U25
Size: 5 × 5 µg