Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD4, clone RM4-5, Pacific Green™ dye conjugate is a monoclonal rat antibody that recognizes the mouse CD4 antigen and the expressed product of the CD4 gene. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD4, Rat IgG2a monoclonal Label: Pacific Green™ dye Validated Applicat...
Catalog: C11207
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD45, clone HI30, Pacific Green™ dye conjugate is a monoclonal mouse antibody that is reactive to the human CD45 antigen and recognizes the expressed product of the PTPRC gene. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD45, Mouse IgG1 monoclonal Label: Pacific Green™ ...
Catalog: C11208
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD45RA, clone MEM-56, Pacific Green™ conjugate is a monoclonal mouse antibody that recognizes the CD45RA antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD45RA, Mouse IgG2b monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity...
Catalog: C11209
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD19, clone SJ25-C1, Pacific Green™ dye conjugate is a monoclonal mouse antibody that recognizes the CD19 antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD19, Mouse IgG1 monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity: ...
Catalog: C11210
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD14, clone TüK4, Pacific Green™ dye conjugate is a monoclonal mouse antibody that recognizes the CD14 antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD14, Mouse IgG2a monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity: Hu...
Catalog: C11211
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD3, clone UCHT1, Pacific Green™ dye conjugate is a monoclonal antibody that is reactive to the human CD3 antigen and recognizes the expressed product of the CD3D gene. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD3, Mouse IgG1 monoclonal Label: Pacific Green™ dye Valid...
Catalog: C11213
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD8A, clone 5H10, Pacific Green™ dye conjugate is a monoclonal mouse antibody that is reactive to the mouse CD8α (Ly-2) antigen. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD8α, Rat IgG2b monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species...
Catalog: C11214
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD4, clone S3.5, Pacific Green™ conjugate is a monoclonal mouse antibody that recognizes the CD4 antigen also known as T4 and L3T4. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD4, Mouse IgG2a monoclonal Label: Pacific Green™ Validated Application: Flow Cytometry Species...
Catalog: C11215
Size: 125 µl
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD3, clone S4.1, Pacific Green™ dye conjugate is a monoclonal antibody that is reactive to the human CD3 antigen.The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: CD3, Mouse IgG2a monoclonal Label: Pacific Green™ dye Validated Application: Flow Cytometry Species Reactivity: Hu...
Catalog: C11216
Size: 125 µl
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2110
Size: 5 mg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2925
Size: 1 mg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2927
Size: 1 mg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C34551
Size: 20 × 50 µg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C34552
Size: 20 × 50 µg
Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells
One Shot® TOP10 E. coli are convenient to use chemically competent cells quality controlled for a transformation efficiency of 1 x 10 9 cfu/ µg plasmid DNA. One Shot® TOP10 Chemically Competent cells are the same competent cells that come with many of our cloning kits and are ideal for high-efficiency cloning and plasmid propagation. • Maximizes cloning efficiency in single tube format • Enhanced genomic DNA cloning capabilities Easy-to-use, Single Tube Format One Shot® TOP10 E. coli are provided at a transformation efficiency of 1 x 10 9 cfu/ µg supercoiled DNA and allow stable replication of...
Catalog: C4040-06
Size: 40 reactions
Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells
TOP10 E. coli are provided at a transformation efficiency of 1 x 10e 9 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids. The genotype of TOP10 cells is similar to the DH10B™strain, and offers the following features: -hsd R for efficient transformation of unmethylated DNA from PCR amplifications -mcrA for efficient transformation of methylated DNA from genomic preparations -lacZΔM15 for blue/white color screening of recombinant clones -endA1 for cleaner preparations of DNA and better results in d...
Catalog: C4040-10
Size: 10 reactions
Category: Products & Services > Competent Cells & Strains > Expression Competent Cells
BL21(DE3) E. coli are ideal for use with bacteriophage T7 promoter-based expression systems ( e.g. , pRSET, pCR¤T7, and pET). BL21(DE3) carry the lambda DE3 lysogen. Recombinant proteins that are non-toxic to E. coli are generally expressed at higher levels in BL21(DE3) cells than in BL21(DE3)pLysS or BL21(DE3)pLysE. However, the basal expression levels of heterologous genes are significantly higher in BL21(DE3) than in BL21(DE3)pLysS or BL21(DE3)pLysE.
Catalog: C6000-03
Size: 20 × 50 µl
Category: Products & Services > Competent Cells & Strains > Expression Competent Cells
One Shot® BL21 Star™ (DE3) E. coli are chemically competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., Champion™ pET vectors) . One Shot® BL21 Star™ (DE3) Chemically Competent cells are controlled for a transformation efficiency of 1 x 10 8 cfu/ µg plasmid DNA. • A genotype that promotes high mRNA stability and protein yield • Ideal for use with low copy number, T7 promoter-based plasmids • The single tube, single use format allows all transformation steps to take place i...
Catalog: C6010-03
Size: 20 reactions
Category: Products & Services > Competent Cells & Strains > Expression Competent Cells
One Shot® BL21-AI™ E. coli are chemically competent cells designed for applications that require tight regulation and strong expression of toxic proteins from any T7 promoter-based expression systems. One Shot® BL21-AI™ Chemically Competent cells have a transformation efficiency of >1×10 8 cfu/ µg plasmid DNA. • Ideal for inducing expression of toxic protein • Gene expression is regulated by the addition of L-arabinose to culture • Suitable for use with any T7 promoter-based plasmids • Supplied in a convenient One Shot® format Excellent Expression of Toxic Recombinant Proteins One Shot® BL21-A...
Catalog: C6070-03
Size: 20 × 50 µl
Category: Products & Services > Competent Cells & Strains > Expression Competent Cells
BL21(DE3)pLysE E. coli are ideal for use with T7 promoter-based expression vectors. Like BL21(DE3)pLysS E. coli , BL21(DE3)pLysE carry the lambda DE3 lysogen. In addition, BL21(DE3)pLysE cells contain the pLysE plasmid, which constitutively expresses T7 lysozyme. T7 lysozyme reduces the basal expression of target genes by inhibiting T7 RNA polymerase. The pLysE plasmid in BL21(DE3)pLysE expresses higher levels of T7 lysozyme than the pLysS plasmid in BL21(DE3)pLysS. The BL21(DE3)pLysE strain provides tighter control of T7 RNA polymerase, which is necessary when the recombinant protein to be ex...
Catalog: C6565-03
Size: 20 × 50 µl
Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells
MC1061/P3 competent E. coli are used for highly efficient transformation of vectors that require the P3 episome for selection and maintenance (i.e., pCDM8, pcDNA™1.1, or any other sup F-containing vector).
Catalog: C663-03
Size: 1.5 ml
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Reactive Oxygen & Nitrogen Species
CM-H 2 DCFDA is a chloromethyl derivative of H 2 DCFDA, useful as an indicator for reactive oxygen species (ROS) in cells. This indicator exhibits much better retention in live cells than H 2 DCFDA. CM-H 2 DCFDA passively diffuses into cells, where its acetate groups are cleaved by intracellular esterases and its thiol-reactive chloromethyl group reacts with intracellular glutathione and other thiols. Subsequent oxidation yields a fluorescent adduct that is trapped inside the cell, thus facilitating long-term studies. ROS Indicator Specifications: • Ex/Em: ~492–495/517–527 nm • Product is air ...
Catalog: C6827
Size: 20 × 50 µg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C7025
Size: 20 × 50 µg
Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells
The One Shot® Stbl3™ E. coli strain is designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli , these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower™ Lentiviral Expression Vector and other retroviral vectors. The DNA yield from Stbl3™ cells is often >10-fold higher than alternative strains such as TOP10 (Table 1). One Shot® Stbl3™ Chemically Competent Cells deliver: • Optimal performance for the propagation of lentiviral vectors • Enhanced genomic cloning capacity ( mcr B, mrr ) • Ex...
Catalog: C7373-03
Size: 20 × 50 µl
Category: Products & Services > Competent Cells & Strains > Cloning Competent Cells
The OmniMAX™ 2 T1 Phage-Resistant (T1 R ) E. coli strain is an improved chemically competent cell line, perfect for use in all cloning applications, including the Gateway® Technology. OmniMAX™ 2 -T1 R cells offer the highest transformation efficiency of any chemically competent cells in a One Shot® format with >5 x 10 9 transformants/µg pUC19. They also provide efficient transformation of highly methylated DNA, since OmniMAX™ 2 -T1 R cells lack the E. coli K12 restriction systems (mc rA Δ (mrr hs dRMS- mc rBC)). In addition, the strain carries the to nA genotype, which confers resistance to T1...
Catalog: C8540-03
Size: 20 transformations
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Campylobacter spp. Real-Time PCR Kit (CAMPP/50) TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only ...
Catalog: CAMPP50
Size: 50 tests
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
CD32, Mouse Anti-Human, (FITC); FITC conjugated Monoclonal antibody specific to Human CD32. This antibody is validated for use in Flow Cytometry. Anti-CD32 recognizes the expressed product of the FCGR2A gene.
Catalog: CD3201
Size: 0.5 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
CD32, Mouse Anti-Human, (R-PE); R-PE conjugated Monoclonal antibody specific to Human CD32. This antibody is validated for use in Flow Cytometry. Anti-CD32 recognizes the expressed product of the FCGR2A gene.
Catalog: CD3204
Size: 0.5 ml
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Same as TaqVet Campylobacter fetus Real-Time PCR Kit (CFP/50) TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only f...
Catalog: CFP50
Size: 50 tests
Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits
Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI™ VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits) • 1 external positive control of the same nature as the target (inactivated bacteria or virus). Us...
Catalog: CHLAB50
Size: 50 tests