Products from Xba I to Soybean Trypsin Inhibitor, Powder
5´-T↓CTAGA-3´ 3´-AGATC↑T-5´ Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns. Concentration 8-20 U/ µL Contents Xba I 10X Buffer M (1mL) 10X Loading Buffer (1mL) Reaction Temperature 37°C Reaction Mixture Xba I 1 µL 10X Buffer M 2 µL 0.10% BSA 2 µL Substrate DNA Heat inactivation Enzyme inactivated by heating at 65°C for 20 minutes....
Catalog: 15226-012
Size: 2,000 units
Size: 2,000 units
5´-CCC↓GGG-3´ 3´-GGG↑CCC-5´ Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns. Concentration 4-12 U/ µL Contents Sma I 10X Buffer T (1mL) 10X Loading Buffer (1mL) Reaction Temperature 30°C Reaction Mixture Sma I 1 µL 10X Buffer T 2 µL 0.10% BSA 2 µL Substrate DNA Heat inactivation Enzyme inactivated by heating at 65°C for 20 minutes....
Catalog: 15228-018
Size: 1,000 units
Size: 1,000 units
Gibco® Distilled Water is high quality water for use as a solvent in the preparation of cell culture media and laboratory reagents. Available in many sizes to meet your needs, Gibco® Distilled Water features: • Cell culture quality • Proven performance • Use in cell culture and molecular biology applications Cell culture quality Gibco® Distilled Water is prepared by distillation and filtration (0.1 µm), with no added substances. Life Technologies monitors the water quality on a routine basis. Conductivity, total organic carbon, endotoxin and bioburden are tested daily; metals are tested monthl...
Catalog: 15230-071
Size: 100 ml
Size: 100 ml
7.5 g/100 ml Prepared with Bovine Serum Albumin Fraction V in phosphate-buffered saline.
Catalog: 15260-037
Size: 100 ml
Size: 100 ml
Fungizone® antimycotic is a formulation of Amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension. Amphotericin B is used to prevent the growth of fungi by causing an increase in fungal plasma membrane permeability. Gibco® Fungizone® antimycotic is used for the prevention of yeast and multicellular fungi contamination of cell cultures. Gibco® Fungizone contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per ml in distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/ml. Life Technologies offers a variety of antibi...
Catalog: 15290-018
Size: 20 ml
Size: 20 ml
Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B™ E. coli , also has 14 non-selectable unique restriction endonuclease recognition sites. Unique cleavage sites for Hin d III and Cla I are found in the promoter of the tetracycline resistance gene. Insertion of DNA at either of these two sites usually results in loss of tetracycline resistance. Performance and Quality Testing: Purity, DNA structure, and selected restriction endonuclease fragmentation...
Catalog: 15367-014
Size: 25 µg
Size: 25 µg
Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing This Trypsin is modified as follows: With Without • EDTA • Phenol Red
Catalog: 15400-054
Size: 100 ml
Size: 100 ml
Yeast tRNA is suitable for use as a carrier in nucleic acid purification and precipitation procedures. It is supplied in lyophilized form.
Catalog: 15401-011
Size: 25 mg
Size: 25 mg
UltraPure™ Phenol is used in the purification of nucleic acids. In a mixture of phenol and buffered aqueous solution, proteins are denatured and collect at the interphase while most nucleic acids remain in the aqueous phase. UltraPure™ Phenol contains no preservatives. It is redistilled and packaged under a dry inert gas in shatter-resistant, plastic-coated amber bottles. Performance and Quality Testing: Color of water-saturated samples is evaluated after incubation at 2°C to 8°C
Catalog: 15509-037
Size: 500 g
Size: 500 g
IPTG (isopropylthio-β-galactoside) is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures.
Catalog: 15529-019
Size: 1 g
Size: 1 g
UltraPure™ Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations. It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas (1).
Catalog: 15535-016
Size: 500 g
Size: 500 g
UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and Quality Testing: No DNase, RNase, or protease activity detected
Catalog: 15567-027
Size: 1 L
Size: 1 L
UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and Quality Testing: No DNase, RNase, or protease activity detected
Catalog: 15568-025
Size: 1 L
Size: 1 L
UltraPure™ Ethidium Bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and Caesium chloride (CsCl) gradients. It is available as an aqueous solution containing 10 mg/ml UltraPure™ Ethidium Bromide and high-purity water. • Sensitive: Detect as little as little as 1 ng of nucleic acids in agarose gels • Versatile: Detect DNA or RNA • Easy to Use: Can be used as-is or diluted to the desired concentration UltraPure™ Ethidium Bromide is an aromatic compound with a phenanthridine core (see structure below). Ethidium bromide has excitation maxima at 300 nm and 520 nm ...
Catalog: 15585-011
Size: 10 ml
Size: 10 ml
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl. When mixtures are extracted with Phenol:Chloroform:Isoamyl Alcohol, proteins are denatured and collected in the organic phase or at the interphase, while nucleic acids remain in the aqueous phase. UltraPure™ Phenol:Chloroform:lsoamyl Alcohol contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated amber bottles. Performance and Quality Te...
Catalog: 15593-031
Size: 100 ml
Size: 100 ml
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl. When mixtures are extracted with Phenol:Chloroform:Isoamyl Alcohol, proteins are denatured and collected in the organic phase or at the interphase, while nucleic acids remain in the aqueous phase. UltraPure™ Phenol:Chloroform:lsoamyl Alcohol contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated amber bottles. Performance and Quality Te...
Catalog: 15593-049
Size: 400 ml
Size: 400 ml
TRIzol® Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour. Key Features of TRIzol® Reagent: • Isolate RNA, DNA, and protein from the same sample • Superior lysis capability, even with difficult sample types • Optimized formulations and pro...
Catalog: 15596-026
Size: 100 ml
Size: 100 ml
0.1-2 Kb RNA Ladder is suitable for sizing single-stranded RNA from 0.1 to 2 kb in glyoxal or formaldehyde agarose gels. The ladder consists of ten bands in even increments (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.8, 1.0, 1.5, and 2.0 kb) to simplify size estimation. The 0.3 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-100
Size: 75 µg
Size: 75 µg
0.5-10 Kb RNA Ladder is suitable for sizing single-stranded RNA ranging from 0.5 kb to 10 kb in glyoxal or formaldehyde agarose gels. The ladder consists of nine bands in even increments (0.5, 1, 1.5, 2, 3, 4, 6, 8, and 10 kb) to simplify size estimation. The 1.5 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-200
Size: 75 µg
Size: 75 µg
Gibco® HEPES (N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10 – 25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO 2 incubator. HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance. Product Use For in vitro diagnostic use. Dual-site cGMP...
Catalog: 15630-130
Size: 20 × 100 ml
Size: 20 × 100 ml
Enrich epithelial tumor cells directly from whole blood, bone marrow, or PBMC suspensions. Enriched tumor cells are lysed for mRNA isolation and RT-PCR amplification. The sensitivity of combined enrichment and RT-PCR detection can be as high as 1 tumor cell in 1 ml blood. Depletion of epithelial tumor cells from whole blood/bone marrow or PBMC. Positive isolation of epithelial tumor cells for downstream molecular analysis. No columns required Starting samples: Whole blood, bone marrow, PBMC, buffy coat. Number of cells processed: 200 ml whole blood or ~4 × 10 9 PBMC.
Catalog: 16102
Size: 5 ml
Size: 5 ml
Sera Category: Secure Origin: New Zealand. Collected from cattle typically 12-24 months of age.
Catalog: 16170-078
Size: 500 ml
Size: 500 ml
Sera Category: Specialty Origin: New Zealand.
Catalog: 16210-064
Size: 100 ml
Size: 100 ml
The FreeStyle™ MAX System is a breakthrough technology for rapid and high-yield mammalian protein production. This new mammalian expression technology is available in two convenient formats. FreeStyle™ MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle™ cells with high efficiency. The FreeStyle™ MAX Expression System combines GIBCO® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293 cells. The only equipment required to use the FreeStyle™ MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinne...
Catalog: 16447-500
Size: 15 ml
Size: 15 ml
UltraPure™ Agarose is a polysaccharide (see structure below) used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to > 20 kb. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved Packaging The new environmentally ...
Catalog: 16500100
Size: 100 g
Size: 100 g
UltraPure™ Agarose is a polysaccharide (see structure below) used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to > 20 kb. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved Packaging The new environmentally ...
Catalog: 16500500
Size: 500 g
Size: 500 g
UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Low Melting Point Agarose is ideal for resolving DNA and RNA fragments larger than 1,000 bp and for the recovery of nucleic acid fragments after electrophoresis, as it melts at 65.5°C (below the melting point of most nucleic acids). The solution remains fluid at 37°C and will set rapidly at temperatures below 25°C. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handlin...
Catalog: 16520100
Size: 100 g
Size: 100 g
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. This McCoy's 5A is modified as follows: With Without • High Glucose • Sodium Pyruvate • L-glutamine • HEPES • Bacto-peptone • Phenol Red
Catalog: 16600-082
Size: 500 ml
Size: 500 ml
with L-Glutamine, EGF and BPE. Shelf life after supplementation: 2 weeks
Catalog: 17005-075
Size: 1 kit
Size: 1 kit
Soybean Trypsin Inhibitor is one of several protease inhibitors found in soybeans. Trypsin is inhibited at a molar ratio of 1:1, while chymotrypsin and plasmin are inhibited to a lesser extent. Soybean trypsin inhibitor will also inhibit other serine proteases. Gibco® Soybean Trypsin Inhibitor, powder has an activity greater than 7,000 BAEE units per mg. A 0.5 mg/mL Soybean Trypsin Inhibitor stock solution should be made by dissolving the non-sterile powder in 0.01M Borate Buffer or Dulbecco's Phosphate Buffered Saline (DPBS), without calcium and magnesium. Filter sterilize the stock solution ...
Catalog: 17075-029
Size: 1 g
Size: 1 g
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