Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Fumarate Hydratase (FH), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of human FH (P07954-1) (Ala 45-Lys 510) fused to a polyhistidine tag at the C-terminus and an initial Met at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 10 mM Tris, 5 mM EDTA, 1 mM DTT, pH 7.5, 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% T...
Catalog: 12115-H08E-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Fat mass and Obesity associated (FTO), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human FTO (Q9C0B1-1) (Lys 2-Pro 505) fused to a polyhistide tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, pH 8.0, 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile doubl...
Catalog: 12125-H07E-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Fat mass and Obesity associated (FTO), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human FTO (Q9C0B1-1) (Lys 2-Pro 505) fused to a polyhistide tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, pH 8.0, 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile doubl...
Catalog: 12125-H07E-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Fat mass and Obesity associated (FTO), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human FTO (Q9C0B1-1) (Lys 2-Pro 505) fused to a polyhistide tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, pH 8.0, 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile doubl...
Catalog: 12125-H07E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Other Enzymes
Fat mass and Obesity associated (FTO), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human FTO (Q9C0B1-1) (Lys 2-Pro 505) fused to a polyhistide tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, pH 8.0, 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile doubl...
Catalog: 12125-H07E-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA Polymerase from Thermococcus species KOD with proofreading (3´a5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic hot-start method that improves PCR specificity (2,3) (Figure 1). PCR x Enhancer Solution is included for higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of Platinum® Pfx DNA Polymera...
Catalog: 11708-013
Size: 100 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
This high-purity Ambion® Taq Polymerase is isolated from an E. coli strain that over-expresses this gene. Its heat stability allows DNA polymerization in the 5' to 3' direction during repeated heat denaturation and reannealing steps in the presence of dNTPs, a DNA template, and complementary primers. It is supplied in one tube containing 250 U at a concentration of 5 U/ µL. It can be used in PCR and RT-PCR reactions. Unit Definition: One unit incorporates 10 nmol of deoxynucleotides into acid insoluble material in 30 min at 74°C. SuperTaq ™ is a trademark of, and is manufactured by Enzyme Tech...
Catalog: AM2052
Size: 250 units
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1). PCR SuperMix contains recombinant Taq DNA polymerase for routine PCR of fragments up to 5kb.
Catalog: 10572-014
Size: 100 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase that requires thermal activation. Upon thermal activation of the inactive enzyme, the modifier is permanently released and the active enzyme is regenerated. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques. • Specificity —Chemical hot-start enzyme for increased specificity, sensitivity, and yield • Sensitivity —Time-release thermal activation improves sensitivity in low copy number amplifications Hot-Start Kinetics for High Sen...
Catalog: N8080241
Size: 250 units
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
Platinum® Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments where high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´a5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase (Table 1). PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. With Platinum® Taq High Fidelity you'll get: Greater than six times higher fidelity than Taq DNA poly-merase Amplification of fragments up to 15 kb (Figure 1) Room temperature reaction...
Catalog: 11304-102
Size: 5000 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
Tfi DNA Polymerase is a new non- Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees. Like Taq , Tfi has 5'→3' exonuclease and 5'→3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness (Figure 1, A and B). Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4 kb in length. In addition, Tfi is free of licensing restrictions that accompany Taq polymerase. Tfi can be considered for use as a co...
Catalog: 30342-052
Size: 5000 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
AccuPrime™ Taq DNA Polymerase High Fidelity amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and proofreading enzyme, and AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime™ Taq DNA Polymerase High Fidelity you'll get: The highest specificity and yield for the most robust PCR amplification 9-fold higher fidelity than Taq DNA polymerase alone Mimimal optimization steps, even with non-optimized primer sets Efficient amplification of targets over a broad size range up...
Catalog: 12346-094
Size: 1000 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
Applied Biosystems® AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus ( Taq ) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy Its thermal activity profile makes it good ...
Catalog: N8080160
Size: 250 units
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > PCR Enzymes & Kits
AccuPrime™ Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accesory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1). AccuPrime™ Pfx SuperMix is ideal for high-fidelity, high-specificity amplification of DNA fragments for applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. Thermostable AccuPrime™ proteins enhance primer-templa...
Catalog: 12344-040
Size: 200 reactions
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Protein Tyrosine Phosphatase Type IVa 2 (PTP4A2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PTP4A2 isoform 1 (Q12974-1) (Asn 2-Gln 167) fused to the GST tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in 50mM Tris, 150mM NaCl, 1mM GSH, pH 7.3, 5% Manni...
Catalog: 13097-H09E-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Protein Tyrosine Phosphatase Type IVa 2 (PTP4A2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PTP4A2 isoform 1 (Q12974-1) (Asn 2-Gln 167) fused to the GST tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in 50mM Tris, 150mM NaCl, 1mM GSH, pH 7.3, 5% Manni...
Catalog: 13097-H09E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Protein Tyrosine Phosphatase, Receptor Type, J (PTPRJ,), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PTPRJ (Q12913) cytoplasmic domain (Arg 997-Ala 1337) fused to a polyhistidinetag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol,...
Catalog: 13165-H07E-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Protein Tyrosine Phosphatase, Receptor Type, J (PTPRJ,), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PTPRJ (Q12913) cytoplasmic domain (Arg 997-Ala 1337) fused to a polyhistidinetag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol,...
Catalog: 13165-H07E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Phosphohistidine Phosphatase 1 (PHPT1), a recombinant human protein, is supplied in frozen format and is quite suitable for use in protein structure analyses. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding human PHPT1 (Q9NRX4-1) (Ala 2-Tyr 125) that is fused to a polyhistidine tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 200 mM NaCl, 20% glycerol, 2 mM DTT, pH 8.0. Reconstitution: No ...
Catalog: 12473-H07E-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Phosphohistidine Phosphatase 1 (PHPT1), a recombinant human protein, is supplied in frozen format and is quite suitable for use in protein structure analyses. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding human PHPT1 (Q9NRX4-1) (Ala 2-Tyr 125) that is fused to a polyhistidine tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 200 mM NaCl, 20% glycerol, 2 mM DTT, pH 8.0. Reconstitution: No ...
Catalog: 12473-H07E-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Acid Phosphatase, prostate (ACPP), recombinant mouse protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse ACPP isoform 1 (Q8CE08-1) (Met1-Arg 381) fused to a C-terminal polyhistidine tag. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in ster...
Catalog: 51018-M08H-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Acid Phosphatase, prostate (ACPP), recombinant mouse protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse ACPP isoform 1 (Q8CE08-1) (Met1-Arg 381) fused to a C-terminal polyhistidine tag. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in ster...
Catalog: 51018-M08H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Acid Phosphatase, prostate (ACPP), recombinant mouse protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse ACPP isoform 1 (Q8CE08-1) (Met1-Arg 381) fused to a C-terminal polyhistidine tag. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in ster...
Catalog: 51018-M08H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Acid Phosphatase, prostate (ACPP), recombinant mouse protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse ACPP isoform 1 (Q8CE08-1) (Met1-Arg 381) fused to a C-terminal polyhistidine tag. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in ster...
Catalog: 51018-M08H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Alkaline Phosphatase, liver/bone/kidney (ALPL), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human tissue-nonspecific alkaline phosphatase (NP_000469.3) (Met 1-Ser 502) fused to a C-terminal polyhistidine tag. Activity: This recombinant protein has not been tested. Formulation: 25 mM Tris, 0.15 M NaCl, pH 7.5, 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% Tween8...
Catalog: 10440-H08H-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Alkaline Phosphatase, liver/bone/kidney (ALPL), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human tissue-nonspecific alkaline phosphatase (NP_000469.3) (Met 1-Ser 502) fused to a C-terminal polyhistidine tag. Activity: This recombinant protein has not been tested. Formulation: 25 mM Tris, 0.15 M NaCl, pH 7.5, 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% Tween8...
Catalog: 10440-H08H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Alkaline Phosphatase, liver/bone/kidney (ALPL), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human tissue-nonspecific alkaline phosphatase (NP_000469.3) (Met 1-Ser 502) fused to a C-terminal polyhistidine tag. Activity: This recombinant protein has not been tested. Formulation: 25 mM Tris, 0.15 M NaCl, pH 7.5, 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% Tween8...
Catalog: 10440-H08H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Phosphatases
Alkaline Phosphatase, liver/bone/kidney (ALPL), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human tissue-nonspecific alkaline phosphatase (NP_000469.3) (Met 1-Ser 502) fused to a C-terminal polyhistidine tag. Activity: This recombinant protein has not been tested. Formulation: 25 mM Tris, 0.15 M NaCl, pH 7.5, 5% Mannitol, 5% Trehalose, 0.02% Tween80, 5% Mannitol, 5% Trehalose, 0.02% Tween8...
Catalog: 10440-H08H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Proteases & Peptidases
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g. the presence of SDS or urea) • In the presence of metal chelating agents (e.g. EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization. Performance and Quality Testing: End...
Catalog: 4333793
Size: 5 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Proteases & Peptidases
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g. the presence of SDS or urea) • In the presence of metal chelating agents (e.g. EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization. Performance and Quality Testing: End...
Catalog: AM2546
Size: 1.25 ml