Products from Purelink® Pcr Purification Kit to Captureselect™ Igselect Leakage Elisa Kit
PureLink® PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products (Figure 1). The simple PureLink® bind/wash/elute procedure: provides two Binding Buffer options for fragments >100 bp or >300 bp* can be performed in either single column or 96-well plate format takes Two proprietary buffers are supplied to meet the needs of different purification requirements. Use the Binding Buffer for routine purifications of double-stranded DNA fragments from 100 bp to 12 kb. Use Binding Buffer HC for removal of primer...
Catalog: K3100-01
Size: 50 reactions
Size: 50 reactions
The PureLink® HiPure Plasmid Purification Systems are designed to isolate transfection-grade plasmid DNA from E. coli in less than 2 hours. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 ml bacterial culture, at a purity that is comparable to that achieved with two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include: • Labor- and time-saving —No need for extra steps to remove endotoxin and other contaminants • High purity, low endotoxin product —pure enough for mammalian cell transfection • Versatility —p...
Catalog: K2100-07
Size: 25 preps
Size: 25 preps
Fast, simple protocols - Purify your plasmid DNA in less than 2 hours Labor-saving - No need for extra steps to remove endotoxin and other contaminants Megapreps and Gigapreps save time by utilizing vacuum-assisted cartridges PureLink® HiPure Plasmid Purification Systems are designed to isolate plasmid DNA of the highest purity (1) with a wide scale range (Table 1). Simple protocols an anion-exchange resin allow you to purify plasmid DNA (Figure 1) at a quality equivalent to two passes through cesium chloride gradients - the most rigorous method for DNA purification. In less than 2 hours, DNA ...
Catalog: K2100-08
Size: 4 preps
Size: 4 preps
This Ambion® kit is for the purification of bacterial mRNA by removing rRNA from total RNA. Sufficient reagents for 20 mRNA purifications, each from 10 µg of total RNA are provided. Features of the MICROB Express ™ Kit: • Dramatically increase the sensitivity of array analyses and other procedures • Removes >95% of 16S and 23S rRNA from bacterial RNA • Simple procedure takes less than 2 hours • Works with many Gram positive and Gram negative bacteria Rapid Purification of Bacterial mRNA is Now Possible Isolating mRNA from bacteria has been virtually impossible, until now. The MICROB Express ™ ...
Catalog: AM1905
Size: 20 reactions
Size: 20 reactions
This Applied Biosystems® product is designed for the purification of RNA from whole blood for real-time RT-PCR, microarrays, or other high-quality RNA experiments. 12 samples can be processed in about 45 minutes. For use with the ABI PRISM® 6100 Nucleic Acid PrepStation, sufficient reagents are included for 24 purifications. • Extract pure, intact RNA • Isolate more than 6 µg of RNA from 3 mL blood • Simple workflow—less time and fewer steps than other kits (see Figure) • Highly stable RNA, free of contaminating gDNA, and inhibitors; no DNase treatment required • Purified RNA used for the gene...
Catalog: 4378672
Size: 24 preps
Size: 24 preps
The ARCTURUS® Paradise® PLUS FFPE RNA Isolation Kit permits unprecedented gene expression analysis of millions of previously inaccessible samples by unlocking RNA from formalin-fixed, paraffin-embedded (FFPE) tissues. Amplify as little as 5 ng of the isolated fixed RNA using reagents and methods that have been specially optimized to overcome the challenges of cross-linked FFPE templates (Figure 1). Secure Downstream Success with the Paradise® QC Kit Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and th...
Catalog: KIT0312-I
Size: 12 samples
Size: 12 samples
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. • Rapid, Non-Hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and Convenient Column Purification Procedures The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and t...
Catalog: 12183018A
Size: 50 preps
Size: 50 preps
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis. • Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit • Simple magnetic bead workflow—no spin columns • Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors Maximize Total ...
Catalog: 4451894
Size: 96 preps
Size: 96 preps
Use the iPrep™ Trizol® Plus RNA Kit for the highest purity, even from difficult samples such as fatty tissue. This kit provides reproducible RNA sample preparation from up to 50 mg of tissue, 10e7 cultured cells, 10 ml blood, and plant samples. The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.
Catalog: IS-10007
Size: 1 kit
Size: 1 kit
The Ambion® RNAqueous® Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 10 7 -10 8 cells using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications. • Convenient syringe-based filtration • Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through th...
Catalog: AM1912
Size: 50 purifications
Size: 50 purifications
This Ambion® kit is for the rapid, high-throughput purification of RNA from mammalian cells and tissue in 96-well plates. The kit combines an organic extraction with magnetic bead–based purification yielding extremely high-quality RNA suitable for the most stringent applications, such as microarray analysis. The kit includes sufficient reagents for 96 reactions. • Improve gene expression studies with consistent results from experiment to experiment • Combines TRI Reagent® with Ambion's superior magnetic bead-based technology • Streamlined RNA purification, without sacrificing RNA quality or qu...
Catalog: AM1839
Size: 96 purifications
Size: 96 purifications
The Ambion® MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. MEGAclear™ efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Each kit includes sufficient reagents for 20 reactions. • Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components • High recovery from 1 ng to 500 µg of input • Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections • Quick protocol—purify RNA in le...
Catalog: AM1908M
Size: 20 rxns w⁄ Manual
Size: 20 rxns w⁄ Manual
The ARCTURUS® PicoPure® RNA Isolation Kit is designed to recover high-quality total RNA consistently from fewer than ten cells, even from a single cell. This kit is designed to provide consistent, efficient RNA recovery without sacrificing quality. Small volume elution allows you to maximize your recovery of RNA from small numbers of cells for use with your gene expression analysis. Key Product Features: • Greater sensitivty—isolate RNA from even a single cell • Superior efficiency—maximize RNA recovery from samples • Streamlined concentration—elute with small volumes • Flexible application—co...
Catalog: KIT0204
Size: 40 samples
Size: 40 samples
The Ion AmpliSeq™ Dynabeads® Cleanup Module is a magnetic bead-based purification kit used in the construction of RNA libraries for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ sequencer. This module is included in the Ion AmpliSeq™ RNA Library Kit, but may be purchased separately if additional modules are needed. For RNA library construction using the Ion AmpliSeq™ RNA Library Kit, the module is used for cleanup after the cDNA synthesis and PCR amplification steps. The module employs Dynabeads® magnetic beads for DNA adsorption and separation, rather than traditional f...
Catalog: 4482437
Size: 96 reactions
Size: 96 reactions
The Ambion® RecoverAll™ Total Nucleic Acid Isolation Kit is for the extraction of total nucleic acid from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up to four 20 µm sections, or up to 35 mg of unsectioned core samples each. Features of the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE: • Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue • No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon • Typical yields are...
Catalog: AM1975
Size: 40 purifications
Size: 40 purifications
The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Catalog: 12280-050
Size: 50 preps
Size: 50 preps
Our Quant-iT™ OliGreen® ssDNA reagent is an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. This reagent is also available in a kit complete with buffer and oligonucleotide standard ([PRODUCT O11492]).
Catalog: O7582
Size: 1 ml
Size: 1 ml
Using the PicoGreen® dsDNA quantitation assay, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides (1). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. PicoGreen® dsDNA Quantitation Reagents are: Orders of magnitude more sensitive than UV absorbance readings, saving on precious sample (Figure 1) Specific for dsDNA in the presence of equimolar amounts of RNA (Figur...
Catalog: P7581
Size: 1 ml
Size: 1 ml
The RediPlate™ 96 RiboGreen® RNA Quantitation Kit is a rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assay. The microplate has been pre-loaded with RiboGreen® Reagent, which shows bright green fluorescence upon binding to RNA or DNA (1). You simply add buffer and samples to the microplate wells, wait 10 minutes, and then read the fluorescence in any standard fluorescence-based microplate reader. The versatile RediPlate™ 96 design is ideal for either high-throughput quantitation or the measurement of a small number of samples. The RediPlate™ 96 RiboGreen® RNA Quantitat...
Catalog: R32700
Size: 1 kit
Size: 1 kit
The Qubit® (previously known as Quant-iT™) dsDNA BR Assay Kit is designed specifically for use with the Qubit® 2.0 Fluorometer . The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer. Common contaminants, such as salts...
Catalog: Q32850
Size: 100 assays
Size: 100 assays
The CaptureSelect™ AVB Sepharose HP Leakage ELISA Kit detects AVB Sepharose HP affinity ligand contamination of adeno-associated viruses (AAV) purified using AVB Sepharose HP affinity resin (GE Healthcare). Even when covalently attached, small amounts of the AVB Sepharose HP affinity ligand can leach off of the chromatography support and co-elute with the virus particles. This ELISA provides sensitivity to detect AVB Sepharose HP affinity ligand contamination of less than 0.5 ng/mL. This assay is designed to minimize interference and to provide accurate quantitation in the presence of adeno-as...
Catalog: 810280801
Size: 1 assay
Size: 1 assay
The CaptureSelect™ AVB Sepharose HP Leakage ELISA Kit detects AVB Sepharose HP affinity ligand contamination of adeno-associated viruses (AAV) purified using AVB Sepharose HP affinity resin (GE Healthcare). Even when covalently attached, small amounts of the AVB Sepharose HP affinity ligand can leach off of the chromatography support and co-elute with the virus particles. This ELISA provides sensitivity to detect AVB Sepharose HP affinity ligand contamination of less than 0.5 ng/mL. This assay is designed to minimize interference and to provide accurate quantitation in the presence of adeno-as...
Catalog: 810280810
Size: 10 assays
Size: 10 assays
The CaptureSelect™ Alpha-1 AntitrypsinSelect Leakage ELISA Kit detects Alpha-1 AntitrypsinSelect affinity ligand contamination of human Alpha-1 antitrypsin (AAT) purified using Alpha-1 Antitrypsin Select affinity resin (GE Healthcare). Even when covalently attached, small amounts of the Alpha-1 Antitrypsin Select affinity ligand can leach off of the chromatography support and co-elute with the purified product. This ELISA provides sensitivity to detect Alpha-1 Antitrypsin Select affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide ac...
Catalog: 810287001
Size: 1 assay
Size: 1 assay
The CaptureSelect™ Alpha-1 AntitrypsinSelect Leakage ELISA Kit detects Alpha-1 AntitrypsinSelect affinity ligand contamination of human Alpha-1 antitrypsin (AAT) purified using Alpha-1 Antitrypsin Select affinity resin (GE Healthcare). Even when covalently attached, small amounts of the Alpha-1 Antitrypsin Select affinity ligand can leach off of the chromatography support and co-elute with the purified product. This ELISA provides sensitivity to detect Alpha-1 Antitrypsin Select affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide ac...
Catalog: 810287010
Size: 10 assays
Size: 10 assays
The CaptureSelect™ GCSFSelect Leakage ELISA Kit detects IgSelect affinity ligand contamination of human GCSF purified using GCSFSelect affinity resin (GE Healthcare). Even when covalently attached, small amounts of the IgSelect affinity ligand can leach off of the chromatography support and co-elute with the antibody. This ELISA provides sensitivity to detect GCSFSelect affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation in the presence of human GCSF. The CaptureSelect™ GCSFSelect Leakage ELISA Kit can be used ...
Catalog: 810313001
Size: 1 assay
Size: 1 assay
The CaptureSelect™ GCSFSelect Leakage ELISA Kit detects IgSelect affinity ligand contamination of human GCSF purified using GCSFSelect affinity resin (GE Healthcare). Even when covalently attached, small amounts of the IgSelect affinity ligand can leach off of the chromatography support and co-elute with the antibody. This ELISA provides sensitivity to detect GCSFSelect affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation in the presence of human GCSF. The CaptureSelect™ GCSFSelect Leakage ELISA Kit can be used ...
Catalog: 810313010
Size: 10 assays
Size: 10 assays
The CaptureSelect™ IXSelect Leakage ELISA Kit detects IXSelect affinity ligand contamination of human Factor IX, purified using IXSelect affinity resin (GE Healthcare). Even when covalently attached, small amounts of the IXSelect affinity ligand can leach off of the chromatography support and co-elute with the purified product. This ELISA provides the sensitivity to detect IXSelect affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation in the presence of Factor IX and other proteins. The CaptureSelect™ IXSelect Le...
Catalog: 810300001
Size: 1 assay
Size: 1 assay
The CaptureSelect™ IXSelect Leakage ELISA Kit detects IXSelect affinity ligand contamination of human Factor IX, purified using IXSelect affinity resin (GE Healthcare). Even when covalently attached, small amounts of the IXSelect affinity ligand can leach off of the chromatography support and co-elute with the purified product. This ELISA provides the sensitivity to detect IXSelect affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation in the presence of Factor IX and other proteins. The CaptureSelect™ IXSelect Le...
Catalog: 810300010
Size: 10 assays
Size: 10 assays
The CaptureSelect™ IgSelect Leakage ELISA Kit detects IgSelect affinity ligand contamination of human IgG purified using IgSelect affinity resin (GE Healthcare). Even when covalently attached, small amounts of the IgSelect affinity ligand can leach off of the chromatography support and co-elute with the antibody. This ELISA provides sensitivity to detect IgSelect affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation in the presence of human IgG. The CaptureSelect™ IgSelect Leakage ELISA Kit can be used as a tool ...
Catalog: 810083501
Size: 1 assay
Size: 1 assay
The CaptureSelect™ IgSelect Leakage ELISA Kit detects IgSelect affinity ligand contamination of human IgG purified using IgSelect affinity resin (GE Healthcare). Even when covalently attached, small amounts of the IgSelect affinity ligand can leach off of the chromatography support and co-elute with the antibody. This ELISA provides sensitivity to detect IgSelect affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation in the presence of human IgG. The CaptureSelect™ IgSelect Leakage ELISA Kit can be used as a tool ...
Catalog: 810083510
Size: 10 assays
Size: 10 assays
Products from Purelink® Pcr Purification Kit to Captureselect™ Igselect Leakage Elisa Kit
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