Products from Edac, 1-Ethyl-3-(3-Dimethylaminopropyl)Carbodiimide, Hydrochloride to Zenon® Pacific Green™ Mouse Igg1 Labeling Kit
EDAC is a water-soluble carbodiimide that can be used to crosslink biological substances that contain carboxylate acids and primary amines. Protocols for crosslinking molecules with this reagent can be found in Bioconjugate Techniques , by G.T. Hermanson (B-7884).
Catalog: E-2247
Size: 100 mg
Size: 100 mg
Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, first to fluorescein monogalactoside (FMG) and then to highly fluorescent fluorescein. Enzyme-mediated hydorlysis of FDG can be followed by the increase in either absorbance or fluorescence.
Catalog: F-1179
Size: 5 mg
Size: 5 mg
This concentrated Nitro-Block-II™ Enhancer can be used with CSPD® or CDP-Star® chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II™ membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF membranes. • Chemically optimized formulations provide enhanced signal intensity in blotting applications with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates) on nitrocellulose or PVDF membranes. • Enhancer increases signal intensity, allowing decreased signal acquisition time. Increa...
Catalog: T2184
Size: 20 ml
Size: 20 ml
TMB (Tetramethyl Benzidine) chromogen solution may be used as a substrate for horseradish peroxidase (HRP) in ELISAs.
Catalog: 00-2023
Size: 500 ml
Size: 500 ml
Stable DAB is a stable, pre-diluted chromogen system designed to clearly localize tissue, DNA, mRNA, and antigens in in situ hybridization and immunoperoxidase procedures. Stable DAB is a ready-to-use reagent containing the correct amounts of diaminobenzidine and hydrogen peroxide. Stable DAB does not fade, creating a permanent record of results under a standard coverslip. It is color coded red for easy user identification.
Catalog: 750118
Size: 250 ml
Size: 250 ml
TMB (Tetramethyl Benzidine) chromogen solution may be used with horseradish peroxidase (HRP)-based immunodetection procedures.
Catalog: 00-2019
Size: 500 ml
Size: 500 ml
Matched and validated lipid and peptide substrates are available to complement the Adapta® Universal Kinase Assay. The assays developed with these reagents allow for primary or secondary screening of kinase inhibitors.
Catalog: PV5707
Size: 1 ml
Size: 1 ml
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR. This dye has been qualified for use on the ABI PRISM® 7700 with Platinum® Quantitative PCR SuperMix-UDG® , Platinum® Quantitative RT-PCR ThermoScript™ One-Step System, and SuperScript® III Platinum® One-Step qRT-PCR Kit. ROX Reference Dye: Normalizes non-PCR-related fluctuations in fluorescence Provides a stable baseline for multiplex quantitative PCR and RT-PCR Uses existing ROX spectral settings, eliminating the need to re-calibrate the thermal cycler
Catalog: 12223-012
Size: 500 µl
Size: 500 µl
InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you'll: Stain proteins fused to an oligohistidine sequence Eliminate western blotting-detect His-tagged proteins directly in the gel Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner Visualize results in under 3 hours Convenienty provided as one ready-to-use solution, InVision™ His-tag ...
Catalog: LC6030
Size: 500 ml
Size: 500 ml
Pro-Q Diamond phosphoprotein gel stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or phosphoprotein specific antibodies and Western blot analysis. Preparation of the Pro-Q Diamond phosphoprotein gel destaining solution is described in the gel staining protocol, but we also provide the detaining solution premixed for your convenience.
Catalog: P-33310
Size: 1 L
Size: 1 L
Molecular Probes' proprietary Pro-Q Emerald 300 and Pro-Q Emerald 488 Glycoprotein Stain Kits provide the most advanced technology available for detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. In less than three hours, it is possible to detect as little as 300 pg of glycoprotein per band, depending on the degree of glycosylation, making these stains at least 50-fold more sensitive than standard fuchsin staining.
Catalog: P-20495
Size: 1 kit
Size: 1 kit
UltraPure™ Ethidium Bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and Caesium chloride (CsCl) gradients. It is available as an aqueous solution containing 10 mg/ml UltraPure™ Ethidium Bromide and high-purity water. • Sensitive: Detect as little as little as 1 ng of nucleic acids in agarose gels • Versatile: Detect DNA or RNA • Easy to Use: Can be used as-is or diluted to the desired concentration UltraPure™ Ethidium Bromide is an aromatic compound with a phenanthridine core (see structure below). Ethidium bromide has excitation maxima at 300 nm and 520 nm ...
Catalog: 15585-011
Size: 10 ml
Size: 10 ml
The Alexa Fluor 488 hydrazide is a low molecular weight, cell membrane—impermeant, aldehyde-fixable molecule that can be used as a cell tracer. This green-fluorescent polar dye can be loaded into cells by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: A-10436
Size: 1 mg
Size: 1 mg
The amine-reactive Lissamine™ rhodamine B sulfonyl chloride can be used to create red-fluorescent bioconjugates with excitation/emission maxima ~568/583 nm.
Catalog: L-20
Size: 1 g
Size: 1 g
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: C-6164
Size: 10 mg
Size: 10 mg
Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
Catalog: A-30005
Size: 1 mg
Size: 1 mg
The amine-reactive PyMPO succinimidyl ester can be used to create bioconjugates with this environment-sensitive fluorophore.
Catalog: S-6110
Size: 5 mg
Size: 5 mg
The amine-reactive Alexa Fluor 430 carboxylic acid, succinimidyl ester and its conjugates exhibit the rare combination of absorption between 400 nm and 450 nm and fluorescence emission beyond 500 nm (excitation/emission maxima ~434/539 nm).
Catalog: A-10169
Size: 5 mg
Size: 5 mg
This single 5-isomer preparation of our proprietary, amine-reactive dye, Oregon Green 488 carboxylic acid, succinimidyl ester and its conjugates have green fluorescence similar to that of fluorescein but are more photostable. Additionally, Oregon Green 488 dye has a lower pK a than fluorescein (pK a = 4.7 versus 6.4 for fluorescein), making this bright dye less pH sensitive in the physiological range.
Catalog: O-6147
Size: 5 mg
Size: 5 mg
Pacific Green™ Succinimidyl Ester is a violet-excitable (411 nm) dye with an emission maximum at ~500 nm, allowing it to be used with other violet-excitable fluorophores (such as Pacific Blue™ or Pacific Orange™ dye) for multiparameter analysis using a single excitation source. As a class of compounds, succinimidyl esters offer an efficient and easy-to-use means for selectively linking a dye to accessible primary amine groups on proteins, modified nucleic acids, or other biomolecules. The covalent bonds formed are as stable as the peptide bonds used to link the amino acids within proteins. Pac...
Catalog: P11202
Size: 250 µg
Size: 250 µg
Create a perfectly labeled antibody with the SiteClick™ Qdot® 525 Antibody Labeling Kit. This kit replaces the conventional Qdot® 525 Antibody Conjugation Kit (Q22041MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10449
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 565 Antibody Labeling Kit. This kit replaces the conventional Qdot® 565 Antibody Conjugation Kit (Q22031MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10450
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 585 Antibody Labeling Kit. This kit replaces the conventional Qdot® 585 Antibody Conjugation Kit (Q22011MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10451
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 605 Antibody Labeling Kit. This kit replaces the conventional Qdot® 605 Antibody Conjugation Kit (Q22001MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction s...
Catalog: S10469
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ R-PE Antibody Labeling Kit. Unlike conventional antibody labeling kits, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. Depending upon the label, the resulting SiteClick™ labeled antibody can be used in flow cytometry or any other application. Importan...
Catalog: S10467
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 625 Antibody Labeling Kit. This kit replaces the conventional Qdot® 625 Antibody Conjugation Kit (A10197). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction st...
Catalog: S10452
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 655 Antibody Labeling Kit. This kit replaces the conventional Qdot® 655 Antibody Conjugation Kit (Q22021MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction ...
Catalog: S10453
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 705 Antibody Labeling Kit. This kit replaces the conventional Qdot® 705 Antibody Conjugation Kit (Q22061MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction ...
Catalog: S10454
Size: 1 kit
Size: 1 kit
Create a perfectly labeled antibody with the SiteClick™ Qdot® 800 Antibody Labeling Kit. This kit replaces the conventional Qdot® 800 Antibody Conjugation Kit (Q22071MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction ...
Catalog: S10455
Size: 1 kit
Size: 1 kit
Zenon® labeling technology provides a fast, versatile, and reliable method for adding a fluorescent label to an antibody. You need only a small amount of starting material,and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Antibody conjugates formed using Zenon® technology may be used in protocols where a directly labeled primary antibody is suitable, including flow cytometry, imaging, and high-throughput applications. This exclusive Molecular Probes® Zenon® labeling technology greatly simplifies the use of multiple mouse-derived...
Catalog: Z11203
Size: 1 kit
Size: 1 kit
Products from Edac, 1-Ethyl-3-(3-Dimethylaminopropyl)Carbodiimide, Hydrochloride to Zenon® Pacific Green™ Mouse Igg1 Labeling Kit
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